Restriction map for
pFN21AE2153
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BbvCI
BcgI BclI BfrI BglII BlnI
BsaBI BsaI BseAI BsiWI BsmBI
BsmI BspDI BspEI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV FbaI
FseI FspI HindIII HpaI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NdeI NotI NruI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I RsrII SalI SapI
ScaI SexAI SgrAI SnaBI SpeI
SspI SwaI Tru9I Tsp509I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccI 1708 GTCTAC GT^[AC][GT]AC
AcyI 4 GGCGCC G[GA]^CG[CT]C
AflIII 1296 ACATGT A^C[GA][CT]GT
AocI 9 CCTCAGG CC^T[ACGT]AGG
AspI 897 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 831 GGATCC G^GATCC
BbeI 4 GGCGCC GGCGC^C
BbrPI 883 CACGTG CAC^GTG
BbsI 268 GTCTTC GAAGAC(2/6)
BciVI 246 GGATAC GTATCC(6/5)
BpuAI 268 GTCTTC GAAGAC(2/6)
BsaAI 883 CACGTG [CT]AC^GT[GA]
BsaHI 4 GGCGCC G[GA]^CG[CT]C
BsaWI 665 TCCGGT [AT]^CCGG[AT]
BseRI 1538 GAGGAG GAGGAG(10/8)
Bsp1407I 1293 TGTACA T^GTACA
BspHI 1817 TCATGA T^CATGA
BspLU11I 1296 ACATGT A^CATGT
BsrBI 1689 CCGCTC CCGCTC(-3/-3)
BsrGI 1293 TGTACA T^GTACA
BstAPI 1264 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1829 TTCGAA TT^CGAA
BstXI 905 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 831 GGATCC [GA]^GATC[CT]
Bsu36I 9 CCTCAGG CC^T[ACGT]AGG
EagI 1065 CGGCCG C^GGCCG
EarI 1821 GAAGAG CTCTTC(1/4)
EclXI 1065 CGGCCG C^GGCCG
Eco52I 1065 CGGCCG C^GGCCG
Eco81I 9 CCTCAGG CC^T[ACGT]AGG
EcoNI 1534 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1720 ATGCAT ATGCA^T
Hin1I 4 GGCGCC G[GA]^CG[CT]C
HincII 639 GTCAAC GT[CT]^[GA]AC
HindII 639 GTCAAC GT[CT]^[GA]AC
KasI 4 GGCGCC G^GCGCC
Ksp632I 1821 GAAGAG CTCTTC(1/4)
KspI 1359 CCGCGG CCGC^GG
MflI 831 GGATCC [GA]^GATC[CT]
NaeI 364 GCCGGC GCC^GGC
NarI 4 GGCGCC GG^CGCC
NcoI 1139 CCATGG C^CATGG
NgoMIV 364 GCCGGC G^CCGGC
NheI 1338 GCTAGC G^CTAGC
NsiI 1720 ATGCAT ATGCA^T
NspV 1829 TTCGAA TT^CGAA
PflFI 897 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1526 GAGTC GAGTC(4/5)
PmaCI 883 CACGTG CAC^GTG
PmlI 883 CACGTG CAC^GTG
PpuMI 1246 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 750 CGATCG CGAT^CG
RcaI 1817 TCATGA T^CATGA
SacII 1359 CCGCGG CCGC^GG
SbfI 348 CCTGCAGG CCTGCA^GG
SfiI 870 GGCCGTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4 GGCGCC GGC^GCC
SfuI 1829 TTCGAA TT^CGAA
SmaI 80 CCCGGG CCC^GGG
SmlI 334 CTCAAG C^T[CT][GA]AG
Sse8387I 348 CCTGCAGG CCTGCA^GG
SspBI 1293 TGTACA T^GTACA
StuI 1189 AGGCCT AGG^CCT
Tth111I 897 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 831 GGATCC [GA]^GATC[CT]
XmaCI 80 CCCGGG C^CCGGG
XmaI 80 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AccII 115 CGCG CG^CG
1360 CGCG
BglI 679 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
871 GCCGTCCTGGC
BlpI 703 GCTGAGC GC^T[ACGT]AGC
1464 GCTCAGC
Bpu1102I 703 GCTGAGC GC^T[ACGT]AGC
1464 GCTCAGC
BsiEI 750 CGATCG CG[GA][CT]^CG
1065 CGGCCG
BspMI 1044 ACCTGC ACCTGC(4/8)
300 GCAGGT
BstUI 115 CGCG CG^CG
1360 CGCG
CelII 703 GCTGAGC GC^T[ACGT]AGC
1464 GCTCAGC
HgaI 790 GACGC GACGC(5/10)
897 GACGC
MaeII 884 ACGT A^CGT
939 ACGT
MvnI 115 CGCG CG^CG
1360 CGCG
SacI 692 GAGCTC GAGCT^C
1462 GAGCTC
SphI 1653 GCATGC GCATG^C
1722 GCATGC
TfiI 662 GAATC G^A[AT]TC
825 GATTC
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
AlwNI ApaI Asp718I AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI KpnI MaeI MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI