(Linear) (Six-base) MAPSORT of: KIEE2153.seq Check: 5365 from: 1 to: 1839 KIEE2153.orf With 182 enzymes: SgfI * July 15, 2010 18:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 291 1053 1839 Size: 291 762 786 Fragments arranged by size: 786 762 291 AccI GT'mk_AC Cuts at: 0 1709 1839 Size: 1709 130 Acc65I G'GTAC_C Cuts at: 0 755 1670 1798 1839 Size: 755 915 128 41 Fragments arranged by size: 915 755 128 41 AflIII A'CryG_T Cuts at: 0 1296 1839 Size: 1296 543 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 541 570 1839 Size: 541 29 1269 Fragments arranged by size: 1269 541 29 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 238 270 1839 Size: 238 32 1569 Fragments arranged by size: 1569 238 32 AlwNI CAG_nnn'CTG Cuts at: 0 220 543 900 1643 1699 1839 Size: 220 323 357 743 56 140 Fragments arranged by size: 743 357 323 220 140 56 ApaI G_GGCC'C Cuts at: 0 81 107 169 485 852 1064 1114 1571 Size: 81 26 62 316 367 212 50 457 Cuts at: 1571 1607 1839 Size: 36 232 Fragments arranged by size: 457 367 316 232 212 81 62 50 36 26 AvaI C'yCGr_G Cuts at: 0 80 97 205 278 485 619 1606 1839 Size: 80 17 108 73 207 134 987 233 Fragments arranged by size: 987 233 207 134 108 80 73 17 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1751 1784 1839 Size: 1751 33 55 Fragments arranged by size: 1751 55 33 BamHI G'GATC_C Cuts at: 0 831 1839 Size: 831 1008 BanI G'GyrC_C Cuts at: 0 4 624 755 775 796 1041 1053 1204 Size: 4 620 131 20 21 245 12 151 Cuts at: 1204 1670 1783 1798 1839 Size: 466 113 15 41 Fragments arranged by size: 620 466 245 151 131 113 41 21 20 15 12 4 BanII G_rGCy'C Cuts at: 0 81 107 169 485 696 852 962 1064 Size: 81 26 62 316 211 156 110 102 Cuts at: 1064 1114 1466 1571 1607 1839 Size: 50 352 105 36 232 Fragments arranged by size: 352 316 232 211 156 110 105 102 81 62 50 36 26 BbeI G_GCGC'C Cuts at: 0 8 1839 Size: 8 1831 BbsI GAAGACnn'nnnn_ Cuts at: 0 261 1839 Size: 261 1578 BciVI GTATCCnnnnn_n' Cuts at: 0 240 1839 Size: 240 1599 BfrBI ATG'CAT Cuts at: 0 1722 1839 Size: 1722 117 BglI GCCn_nnn'nGGC Cuts at: 0 685 877 1839 Size: 685 192 962 Fragments arranged by size: 962 685 192 BlpI GC'TnA_GC Cuts at: 0 704 1465 1839 Size: 704 761 374 Fragments arranged by size: 761 704 374 Bme1580I G_kGCm'C Cuts at: 0 81 107 169 485 620 780 852 993 Size: 81 26 62 316 135 160 72 141 Cuts at: 993 1056 1064 1114 1207 1275 1571 1607 1635 Size: 63 8 50 93 68 296 36 28 Cuts at: 1635 1689 1839 Size: 54 150 Fragments arranged by size: 316 296 160 150 141 135 93 81 72 68 63 62 54 50 36 28 26 8 BmrI ACTGGGnnnn_n' Cuts at: 0 235 246 559 1839 Size: 235 11 313 1280 Fragments arranged by size: 1280 313 235 11 BmtI G_CTAG'C Cuts at: 0 1342 1839 Size: 1342 497 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1389 1485 1839 Size: 1389 96 354 Fragments arranged by size: 1389 354 96 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 710 1342 1556 1839 Size: 442 268 632 214 283 Fragments arranged by size: 632 442 283 268 214 Bpu10I CC'TnA_GC Cuts at: 0 1692 1839 Size: 1692 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 319 1839 Size: 319 1520 BsaAI yAC'GTr Cuts at: 0 885 1839 Size: 885 954 BsaHI Gr'CG_yC Cuts at: 0 5 1839 Size: 5 1834 BsaWI w'CCGG_w Cuts at: 0 665 1839 Size: 665 1174 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 373 403 1337 1367 1532 1562 1839 Size: 373 30 934 30 165 30 277 Fragments arranged by size: 934 373 277 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1553 1839 Size: 1553 286 BseYI C'CCAG_C Cuts at: 0 74 173 845 999 1079 1481 1521 1636 Size: 74 99 672 154 80 402 40 115 Cuts at: 1636 1839 Size: 203 Fragments arranged by size: 672 402 203 154 115 99 80 74 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 206 319 325 792 913 1669 1839 Size: 206 113 6 467 121 756 170 Fragments arranged by size: 756 467 206 170 121 113 6 BsiEI CG_ry'CG Cuts at: 0 753 1068 1839 Size: 753 315 771 Fragments arranged by size: 771 753 315 BsiHKAI G_wGCw'C Cuts at: 0 335 696 1466 1839 Size: 335 361 770 373 Fragments arranged by size: 770 373 361 335 Bsp1286I G_dGCh'C Cuts at: 0 81 107 169 335 485 620 696 780 Size: 81 26 62 166 150 135 76 84 Cuts at: 780 852 962 993 1056 1064 1114 1207 1275 Size: 72 110 31 63 8 50 93 68 Cuts at: 1275 1466 1571 1607 1635 1689 1839 Size: 191 105 36 28 54 150 Fragments arranged by size: 191 166 150 150 135 110 105 93 84 81 76 72 68 63 62 54 50 36 31 28 26 8 BspHI T'CATG_A Cuts at: 0 1817 1839 Size: 1817 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 291 1053 1839 Size: 291 762 786 Fragments arranged by size: 786 762 291 BsrBI CCG'CTC Cuts at: 0 1691 1839 Size: 1691 148 BsrDI GCAATG_nn' Cuts at: 0 153 984 1133 1839 Size: 153 831 149 706 Fragments arranged by size: 831 706 153 149 BsrFI r'CCGG_y Cuts at: 0 154 364 1166 1839 Size: 154 210 802 673 Fragments arranged by size: 802 673 210 154 BsrGI T'GTAC_A Cuts at: 0 1293 1839 Size: 1293 546 BstAPI GCAn_nnn'nTGC Cuts at: 0 1270 1839 Size: 1270 569 BstBI TT'CG_AA Cuts at: 0 1830 1839 Size: 1830 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 912 1839 Size: 912 927 BstYI r'GATC_y Cuts at: 0 831 1839 Size: 831 1008 Bsu36I CC'TnA_GG Cuts at: 0 10 1839 Size: 10 1829 BtgI C'CryG_G Cuts at: 0 326 680 1139 1359 1839 Size: 326 354 459 220 480 Fragments arranged by size: 480 459 354 326 220 BtsI GCAGTG_nn' Cuts at: 0 602 804 809 923 1689 1839 Size: 602 202 5 114 766 150 Fragments arranged by size: 766 602 202 150 114 5 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1776 1811 1839 Size: 1776 35 28 Fragments arranged by size: 1776 35 28 EaeI y'GGCC_r Cuts at: 0 677 1065 1424 1839 Size: 677 388 359 415 Fragments arranged by size: 677 415 388 359 EagI C'GGCC_G Cuts at: 0 1065 1839 Size: 1065 774 EarI CTCTTCn'nnn_ Cuts at: 0 1816 1839 Size: 1816 23 EcoICRI GAG'CTC Cuts at: 0 694 1464 1839 Size: 694 770 375 Fragments arranged by size: 770 694 375 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 710 1342 1556 1839 Size: 442 268 632 214 283 Fragments arranged by size: 632 442 283 268 214 EcoNI CCTnn'n_nnAGG Cuts at: 0 1538 1839 Size: 1538 301 EcoO109I rG'GnC_Cy Cuts at: 0 103 104 165 166 210 481 482 498 Size: 103 1 61 1 44 271 1 16 Cuts at: 498 928 1060 1111 1247 1543 1567 1568 1603 Size: 430 132 51 136 296 24 1 35 Cuts at: 1603 1696 1839 Size: 93 143 Fragments arranged by size: 430 296 271 143 136 132 103 93 61 51 44 35 24 16 1 1 1 1 HaeII r_GCGC'y Cuts at: 0 8 312 1643 1839 Size: 8 304 1331 196 Fragments arranged by size: 1331 304 196 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 816 848 1532 1564 1839 Size: 816 32 684 32 275 Fragments arranged by size: 816 684 275 32 32 HincII GTy'rAC Cuts at: 0 641 1839 Size: 641 1198 KasI G'GCGC_C Cuts at: 0 4 1839 Size: 4 1835 KpnI G_GTAC'C Cuts at: 0 759 1674 1802 1839 Size: 759 915 128 37 Fragments arranged by size: 915 759 128 37 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 922 1839 Size: 922 917 MslI CAynn'nnrTG Cuts at: 0 523 887 1138 1393 1839 Size: 523 364 251 255 446 Fragments arranged by size: 523 446 364 255 251 MspA1I CmG'CkG Cuts at: 0 42 130 422 817 845 912 1083 1214 Size: 42 88 292 395 28 67 171 131 Cuts at: 1214 1361 1433 1485 1667 1839 Size: 147 72 52 182 172 Fragments arranged by size: 395 292 182 172 171 147 131 88 72 67 52 42 28 NaeI GCC'GGC Cuts at: 0 366 1839 Size: 366 1473 NarI GG'CG_CC Cuts at: 0 5 1839 Size: 5 1834 NcoI C'CATG_G Cuts at: 0 1139 1839 Size: 1139 700 NgoMIV G'CCGG_C Cuts at: 0 364 1839 Size: 364 1475 NheI G'CTAG_C Cuts at: 0 1338 1839 Size: 1338 501 NsiI A_TGCA'T Cuts at: 0 1724 1839 Size: 1724 115 NspI r_CATG'y Cuts at: 0 1300 1657 1722 1726 1839 Size: 1300 357 65 4 113 Fragments arranged by size: 1300 357 113 65 4 PasI CC'CwG_GG Cuts at: 0 478 952 1839 Size: 478 474 887 Fragments arranged by size: 887 478 474 PciI A'CATG_T Cuts at: 0 1296 1839 Size: 1296 543 PflMI CCAn_nnn'nTGG Cuts at: 0 1436 1488 1643 1839 Size: 1436 52 155 196 Fragments arranged by size: 1436 196 155 52 PmlI CAC'GTG Cuts at: 0 885 1839 Size: 885 954 PpuMI rG'GwC_Cy Cuts at: 0 1247 1839 Size: 1247 592 PspOMI G'GGCC_C Cuts at: 0 77 103 165 481 848 1060 1110 1567 Size: 77 26 62 316 367 212 50 457 Cuts at: 1567 1603 1839 Size: 36 236 Fragments arranged by size: 457 367 316 236 212 77 62 50 36 26 PstI C_TGCA'G Cuts at: 0 353 650 720 1839 Size: 353 297 70 1119 Fragments arranged by size: 1119 353 297 70 PvuI CG_AT'CG Cuts at: 0 753 1839 Size: 753 1086 PvuII CAG'CTG Cuts at: 0 130 845 912 1083 1433 1485 1667 1839 Size: 130 715 67 171 350 52 182 172 Fragments arranged by size: 715 350 182 172 171 130 67 52 SacI G_AGCT'C Cuts at: 0 696 1466 1839 Size: 696 770 373 Fragments arranged by size: 770 696 373 SacII CC_GC'GG Cuts at: 0 1362 1839 Size: 1362 477 SanDI GG'GwC_CC Cuts at: 0 1247 1839 Size: 1247 592 SbfI CC_TGCA'GG Cuts at: 0 353 1839 Size: 353 1486 SfcI C'TryA_G Cuts at: 0 349 417 646 716 1025 1662 1839 Size: 349 68 229 70 309 637 177 Fragments arranged by size: 637 349 309 229 177 70 68 SfiI GGCCn_nnn'nGGCC Cuts at: 0 877 1839 Size: 877 962 SfoI GGC'GCC Cuts at: 0 6 1839 Size: 6 1833 SmaI CCC'GGG Cuts at: 0 82 1839 Size: 82 1757 SmlI C'TyrA_G Cuts at: 0 334 1839 Size: 334 1505 SphI G_CATG'C Cuts at: 0 1657 1726 1839 Size: 1657 69 113 Fragments arranged by size: 1657 113 69 StuI AGG'CCT Cuts at: 0 1191 1839 Size: 1191 648 StyI C'CwwG_G Cuts at: 0 242 962 1139 1794 1839 Size: 242 720 177 655 45 Fragments arranged by size: 720 655 242 177 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 763 1428 1480 1635 1839 Size: 763 665 52 155 204 Fragments arranged by size: 763 665 204 155 52 TatI w'GTAC_w Cuts at: 0 1293 1839 Size: 1293 546 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 373 405 958 990 1337 1369 1839 Size: 373 32 553 32 347 32 470 Fragments arranged by size: 553 470 373 347 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 900 1839 Size: 900 939 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1146 1437 1489 1839 Size: 1146 291 52 350 Fragments arranged by size: 1146 350 291 52 XmaI C'CCGG_G Cuts at: 0 80 1839 Size: 80 1759 Enzymes that do cut: AarI AccI Acc65I AflIII AlfI AloI AlwNI ApaI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BfrBI BglI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI KpnI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI PmlI PpuMI PspOMI PstI PvuI PvuII SacI SacII SanDI SbfI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AclI AcuI AfeI AflII AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII BbvCI BcgI BclI BglII BmgBI BsaI BsaBI BsiWI BsmI BsmBI BspEI BssHII BssSI BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI MluI MscI NdeI NotI NruI PacI PfoI PmeI PpiI PshAI PsiI PspXI PsrI RsrII SalI SapI ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmnI ZraI