################################## # NO CUT ENZYMES ( 214 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AfaI 130 GTAC GT^AC Asp700I 239 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 379 GAAGAC GAAGAC(2/6) BbvI 88 GCTGC GCAGC(8/12) BfaI 331 CTAG C^TAG BpmI 270 CTCCAG CTGGAG(16/14) BpuAI 379 GAAGAC GAAGAC(2/6) BsaAI 401 TACGTG [CT]AC^GT[GA] BsaJI 353 CCAAGG C^C[ACGT][ACGT]GG BseRI 139 GAGGAG GAGGAG(10/8) BssKI 123 CCTGG ^CC[ACGT]GG BstNI 123 CCTGG CC^[AT]GG DdeI 52 CTGAG C^T[ACGT]AG EarI 239 GAAGAG CTCTTC(1/4) EcoRII 123 CCTGG ^CC[AT]GG EcoT14I 353 CCAAGG C^C[AT][AT]GG Fnu4HI 88 GCTGC GC^[ACGT]GC HinfI 7 GAGTC G^A[ACGT]TC HphI 157 TCACC GGTGA(8/7) ItaI 88 GCTGC GC^[ACGT]GC Ksp632I 239 GAAGAG CTCTTC(1/4) MaeI 331 CTAG C^TAG MaeII 402 ACGT A^CGT MaeIII 399 GTTAC ^GT[ACGT]AC MseI 170 TTAA T^TAA MslI 229 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 341 CAGCTG C[AC]G^C[GT]G MvaI 123 CCTGG CC^[AT]GG NlaIII 229 CATG CATG^ PleI 7 GAGTC GAGTC(4/5) PvuII 341 CAGCTG CAG^CTG RsaI 130 GTAC GT^AC ScrFI 123 CCTGG CC^[ACGT]GG SmlI 145 CTTGAG C^T[CT][GA]AG StyI 353 CCAAGG C^C[AT][AT]GG Tru9I 170 TTAA T^TAA TseI 88 GCTGC G^C[AT]GC XmnI 239 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 3 enzymes) # ################################### BsrI 132 ACTGG ACTGG(1/-1) 272 CCAGT BstF5I 135 GGATG GGATG(2/0) 283 GGATG FokI 135 GGATG GGATG(9/13) 283 GGATG ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### AluI MboII MnlI