################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstBI BstYI BstZ17I ClaI CpoI DpnII DraI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI HindIII HinfI HpaI KpnI MamI MboI MfeI MflI MluI MseI MslI MunI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI Sau3AI SbfI ScaI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 644 TCCGGA T^CCGGA AfaI 1080 GTAC GT^AC AocI 1417 CCTGAGG CC^T[ACGT]AGG AscI 86 GGCGCGCC GG^CGCGCC AviII 626 TGCGCA TGC^GCA BalI 296 TGGCCA TGG^CCA BbeI 478 GGCGCC GGCGC^C BcgI 735 CGAGGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1282 CTAG C^TAG BseAI 644 TCCGGA T^CCGGA BsiEI 573 CGACCG CG[GA][CT]^CG BsmBI 895 GAGACG CGTCTC(1/5) BspEI 644 TCCGGA T^CCGGA BsrBI 61 GAGCGG CCGCTC(-3/-3) BssSI 850 CTCGTG CACGAG(-5/-1) BstEII 1470 GGTGACC G^GT[ACGT]ACC BstPI 1470 GGTGACC G^GT[ACGT]ACC Bsu36I 1417 CCTGAGG CC^T[ACGT]AGG DraIII 451 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1285 GACCGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 202 CTCTTC CTCTTC(1/4) Eco81I 1417 CCTGAGG CC^T[ACGT]AGG EcoO65I 1470 GGTGACC G^GT[ACGT]ACC FspI 626 TGCGCA TGC^GCA HincII 496 GTCAAC GT[CT]^[GA]AC HindII 496 GTCAAC GT[CT]^[GA]AC KasI 478 GGCGCC G^GCGCC Ksp632I 202 CTCTTC CTCTTC(1/4) MaeI 1282 CTAG C^TAG MaeII 710 ACGT A^CGT MluNI 296 TGGCCA TGG^CCA MroI 644 TCCGGA T^CCGGA MscI 296 TGGCCA TGG^CCA NarI 478 GGCGCC GG^CGCC RsaI 1080 GTAC GT^AC SacI 1180 GAGCTC GAGCT^C SexAI 452 ACCTGGT A^CC[AT]GGT SfiI 603 GGCCGCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 478 GGCGCC GGC^GCC SmaI 608 CCCGGG CCC^GGG SmlI 1522 CTCAAG C^T[CT][GA]AG TaqI 599 TCGA T^CGA TthHB8I 599 TCGA T^CGA XcmI 1479 CCAGCCTGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 608 CCCGGG C^CCGGG XmaI 608 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwNI 364 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 541 CAGACGCTG Aor51HI 775 AGCGCT AGC^GCT 992 AGCGCT ApaI 110 GGGCCC GGGCC^C 1423 GGGCCC BanI 478 GGCGCC G^G[CT][GA]CC 831 GGTGCC BlpI 1305 GCTGAGC GC^T[ACGT]AGC 1444 GCTGAGC Bpu1102I 1305 GCTGAGC GC^T[ACGT]AGC 1444 GCTGAGC BsaWI 644 TCCGGA [AT]^CCGG[AT] 1286 ACCGGA BsrFI 650 GCCGGC [GA]^CCGG[CT] 1024 GCCGGC BssHII 87 GCGCGC G^CGCGC 975 GCGCGC BstF5I 642 CATCC GGATG(2/0) 47 GGATG CelII 1305 GCTGAGC GC^T[ACGT]AGC 1444 GCTGAGC Cfr10I 650 GCCGGC [GA]^CCGG[CT] 1024 GCCGGC Eco47III 775 AGCGCT AGC^GCT 992 AGCGCT EcoNI 189 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 222 CCTTCTGGAGG FokI 642 CATCC GGATG(9/13) 47 GGATG KspI 812 CCGCGG CCGC^GG 1032 CCGCGG NaeI 650 GCCGGC GCC^GGC 1024 GCCGGC NcoI 35 CCATGG C^CATGG 566 CCATGG NgoMIV 650 GCCGGC G^CCGGC 1024 GCCGGC PstI 655 CTGCAG CTGCA^G 1402 CTGCAG PvuII 1303 CAGCTG CAG^CTG 1442 CAGCTG SacII 812 CCGCGG CCGC^GG 1032 CCGCGG SfcI 655 CTGCAG C^T[GA][CT]AG 1402 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV NspI PflMI PpuMI Sau96I ScrFI SfaNI SphI StyI TseI Tsp45I TspRI Van91I