Restriction map for
pFN21AE1964
(Circular) (Six-base) MAPSORT of: pFN21AE1964.seq Check: 5823 from: 1 to: 6289
pFN21AE1964 6289 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2092 2450 2460 2092
Size: 358 10 5921
Fragments arranged by size:
5921 358 10
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4099 278
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3632 1937
Size: 1695 4594
Acc65I G'GTAC_C
Cuts at: 3610 3610
Size: 6289
AclI AA'CG_TT
Cuts at: 1563 4416 4789 1563
Size: 2853 373 3063
Fragments arranged by size:
3063 2853 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2216 2975 3401 3484 4352 5736 6004
Size: 461 123 759 426 83 868 1384 268
Cuts at: 6004 1632
Size: 1917
Fragments arranged by size:
1917 1384 868 759 461 426 268 123
83
AfeI AGC'GCT
Cuts at: 2782 2999 2782
Size: 217 6072
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6092
AflIII A'CryG_T
Cuts at: 1203 5188 6165 1203
Size: 3985 977 1327
Fragments arranged by size:
3985 1327 977
AgeI A'CCGG_T
Cuts at: 5120 5120
Size: 6289
AhdI GACnn_n'nnGTC
Cuts at: 5018 5018
Size: 6289
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6289
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2870 2902 2870
Size: 32 6257
AlwNI CAG_nnn'CTG
Cuts at: 2374 2551 4032 5604 2374
Size: 177 1481 1572 3059
Fragments arranged by size:
3059 1572 1481 177
ApaI G_GGCC'C
Cuts at: 2119 3432 2119
Size: 1313 4976
ApaLI G'TGCA_C
Cuts at: 4346 5502 4346
Size: 1156 5133
ApoI r'AATT_y
Cuts at: 1493 3598 3824 3976 6085 1493
Size: 2105 226 152 2109 1697
Fragments arranged by size:
2109 2105 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2092 2092
Size: 6289
AseI AT'TA_AT
Cuts at: 160 1032 4843 160
Size: 872 3811 1606
Fragments arranged by size:
3811 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
AvaI C'yCGr_G
Cuts at: 1870 1943 2613 3152 3431 3614 1870
Size: 73 670 539 279 183 4545
Fragments arranged by size:
4545 670 539 279 183 73
BamHI G'GATC_C
Cuts at: 3619 3986 3619
Size: 367 5922
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2483 2836 3610 5065 618
Size: 325 217 627 696 353 774 1455 1842
Fragments arranged by size:
1842 1455 774 696 627 353 325 217
BanII G_rGCy'C
Cuts at: 1391 2119 2193 3189 3257 3317 3432 3440 3608
Size: 728 74 996 68 60 115 8 168
Cuts at: 3608 1391
Size: 4072
Fragments arranged by size:
4072 996 728 168 115 74 68 60
8
BbeI G_GCGC'C
Cuts at: 2487 2487
Size: 6289
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5318
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2729 2763 4480 4514 1903
Size: 34 792 34 1717 34 3678
Fragments arranged by size:
3678 1717 792 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4181 5391 4181
Size: 1210 5079
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6289
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2615 3107 3177
Size: 107 122 71 631 605 943 492 70
Cuts at: 3177 4900 136
Size: 1723 1525
Fragments arranged by size:
1723 1525 943 631 605 492 122 107
71 70
BglII A'GATC_T
Cuts at: 6284 6284
Size: 6289
BlpI GC'TnA_GC
Cuts at: 3311 3450 3706 3311
Size: 139 256 5894
Fragments arranged by size:
5894 256 139
Bme1580I G_kGCm'C
Cuts at: 1790 2119 2841 3432 4350 5506 1790
Size: 329 722 591 918 1156 2573
Fragments arranged by size:
2573 1156 918 722 591 329
BmgBI CAC'GTC
Cuts at: 1318 2716 1318
Size: 1398 4891
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2211 2836 3254
Size: 416 53 83 399 24 794 625 418
Cuts at: 3254 4020 4978 442
Size: 766 958 1753
Fragments arranged by size:
1753 958 794 766 625 418 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2252 2396 2582 2969 3007 4949 1239
Size: 800 213 144 186 387 38 1942 2579
Fragments arranged by size:
2579 1942 800 387 213 186 144 38
Bpu10I CC'TnA_GC
Cuts at: 2777 2784 3188 2777
Size: 7 404 5878
Fragments arranged by size:
5878 404 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3512 4414 5279 5577 3512
Size: 902 865 298 4224
Fragments arranged by size:
4224 902 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4952 882
Size: 617 3453 2219
Fragments arranged by size:
3453 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4080 493
Size: 711 2876 2702
Fragments arranged by size:
2876 2702 711
BsaBI GATnn'nnATC
Cuts at: 6283 6283
Size: 6289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2420 2484 2903 2970
Size: 53 83 186 936 887 64 419 67
Cuts at: 2970 4096 4478 5113 275
Size: 1126 382 635 1451
Fragments arranged by size:
1451 1126 936 887 635 419 382 186
83 67 64 53
BsaWI w'CCGG_w
Cuts at: 2649 3291 4722 5120 5394 5541 5952 2649
Size: 642 1431 398 274 147 411 2986
Fragments arranged by size:
2986 1431 642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6064
Fragments arranged by size:
6064 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2942 3221 3580 1186
Size: 1756 279 359 3895
Fragments arranged by size:
3895 1756 359 279
BseYI C'CCAG_C
Cuts at: 2097 2381 2799 3069 3483 5492 5988 2097
Size: 284 418 270 414 2009 496 2398
Fragments arranged by size:
2398 2009 496 418 414 284 270
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2312 2384 2438 2696 2795 1163
Size: 573 576 72 54 258 99 4657
Fragments arranged by size:
4657 576 573 258 99 72 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2581 3739 4500 5528 1540
Size: 275 186 580 1158 761 1028 2301
Fragments arranged by size:
2301 1158 1028 761 580 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 2060 3189 4350 4435 5506 2016
Size: 44 1129 1161 85 1071 2799
Fragments arranged by size:
2799 1161 1129 1071 85 44
BsmI GAATG_Cn'
Cuts at: 3800 3893 3800
Size: 93 6196
BsmBI CGTCTCn'nnnn_
Cuts at: 2894 2894
Size: 6289
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2060 2119 2193 2841 3189 3257
Size: 399 226 44 59 74 648 348 68
Cuts at: 3257 3317 3432 3440 3608 4350 4435 5506 1391
Size: 60 115 8 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 648 399 348 226 168
115 85 74 68 60 59 44 8
BspEI T'CCGG_A
Cuts at: 2649 5952 2649
Size: 3303 2986
BspHI T'CATG_A
Cuts at: 4178 5908 4178
Size: 1730 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2092 2191 2450 2460 3644 844
Size: 1248 99 259 10 1184 3489
Fragments arranged by size:
3489 1248 1184 259 99 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 4176 1394
Size: 429 245 2108 3507
Fragments arranged by size:
3507 2108 429 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 4784 4958 65
Size: 1183 3536 174 1396
Fragments arranged by size:
3536 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2655 3029 4933 5120 1700
Size: 110 845 374 1904 187 2869
Fragments arranged by size:
2869 1904 845 374 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6289
BssHII G'CGCG_C
Cuts at: 1925 2092 2980 1925
Size: 167 888 5234
Fragments arranged by size:
5234 888 167
BssSI C'ACGA_G
Cuts at: 2855 4349 5361 2855
Size: 1494 1012 3783
Fragments arranged by size:
3783 1494 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6289
BstBI TT'CG_AA
Cuts at: 5093 5093
Size: 6289
BstEII G'GTnAC_C
Cuts at: 1184 3475 1184
Size: 2291 3998
BstXI CCAn_nnnn'nTGG
Cuts at: 2276 2494 2947 2276
Size: 218 453 5618
Fragments arranged by size:
5618 453 218
BstYI r'GATC_y
Cuts at: 1972 3619 3986 4371 4388 5840 5926 5938 6284
Size: 1647 367 385 17 1452 86 12 346
Cuts at: 6284 1972
Size: 1977
Fragments arranged by size:
1977 1647 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3423 3423
Size: 6289
BtgI C'CryG_G
Cuts at: 513 1065 2004 2040 2571 2581 2695 2817 3037
Size: 552 939 36 531 10 114 122 220
Cuts at: 3037 3558 513
Size: 521 3244
Fragments arranged by size:
3244 939 552 531 521 220 122 114
36 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2458 487
Size: 683 56 1232 4318
Fragments arranged by size:
4318 1232 683 56
BtsI GCAGTG_nn'
Cuts at: 3805 4599 4619 3805
Size: 794 20 5475
Fragments arranged by size:
5475 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6254
DraI TTT'AAA
Cuts at: 3593 3593
Size: 6289
DraIII CAC_nnn'GTG
Cuts at: 2461 2461
Size: 6289
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3296 5296 809
Size: 507 1980 2000 1802
Fragments arranged by size:
2000 1980 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2301 2436
Size: 54 1425 50 275 18 177 294 135
Cuts at: 2436 2446 2574 3241 3736 4625 6254 8
Size: 10 128 667 495 889 1629 43
Fragments arranged by size:
1629 1425 889 667 495 294 275 177
135 128 54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 3736 1537
Size: 275 1924 4090
Fragments arranged by size:
4090 1924 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2213 4219 1353
Size: 860 2006 3423
Fragments arranged by size:
3423 2006 860
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2255 4876 5250 5396 387
Size: 1064 138 666 2621 374 146 1280
Fragments arranged by size:
2621 1280 1064 666 374 146 138
EcoICRI GAG'CTC
Cuts at: 3187 3187
Size: 6289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2093 2216 2252 2396 2582 2969
Size: 393 407 54 123 36 144 186 387
Cuts at: 2969 2975 3007 3401 3484 4352 4949 5736 6004
Size: 6 32 394 83 868 597 787 268
Cuts at: 6004 1239
Size: 1524
Fragments arranged by size:
1524 868 787 597 407 394 393 387
268 186 144 123 83 54 36 32
6
EcoNI CCTnn'n_nnAGG
Cuts at: 2198 2231 2198
Size: 33 6256
EcoO109I rG'GnC_Cy
Cuts at: 2150 2203 3428 3514 2150
Size: 53 1225 86 4925
Fragments arranged by size:
4925 1225 86 53
EcoRI G'AATT_C
Cuts at: 3598 3976 3598
Size: 378 5911
FspI TGC'GCA
Cuts at: 2633 4795 2633
Size: 2162 4127
HaeII r_GCGC'y
Cuts at: 1678 2487 2784 3001 3030 3388 5436 1678
Size: 809 297 217 29 358 2048 2531
Fragments arranged by size:
2531 2048 809 358 297 217 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4930 4962 5004 5036 1373
Size: 32 135 32 3358 32 42 32 2626
Fragments arranged by size:
3358 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2503 3633 3879 669
Size: 988 281 565 1130 246 3079
Fragments arranged by size:
3079 1130 988 565 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6289
HpaI GTT'AAC
Cuts at: 3879 3879
Size: 6289
KasI G'GCGC_C
Cuts at: 2483 2483
Size: 6289
KpnI G_GTAC'C
Cuts at: 3614 3614
Size: 6289
MfeI C'AATT_G
Cuts at: 3888 3888
Size: 6289
MluI A'CGCG_T
Cuts at: 6165 6165
Size: 6289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5403 5587 1305
Size: 4098 184 2007
Fragments arranged by size:
4098 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 10
Size: 54 1425 343 471 3996
Fragments arranged by size:
3996 1425 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4247 4606 4765 518
Size: 648 3081 359 159 2042
Fragments arranged by size:
3081 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2240 2809 2819 3039 3310 3449 3705 4382
Size: 634 569 10 220 271 139 256 677
Cuts at: 4382 5530 5775 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 634 569 271 256 245
220 139 10
NaeI GCC'GGC
Cuts at: 2657 3031 2657
Size: 374 5915
NarI GG'CG_CC
Cuts at: 2484 2484
Size: 6289
NcoI C'CATG_G
Cuts at: 513 1065 2004 2040 2571 513
Size: 552 939 36 531 4231
Fragments arranged by size:
4231 939 552 531 36
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6289
NgoMIV G'CCGG_C
Cuts at: 2655 3029 2655
Size: 374 5915
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6289
NotI GC'GGCC_GC
Cuts at: 3736 3736
Size: 6289
NspI r_CATG'y
Cuts at: 1132 1229 2081 2432 2521 2638 3647 5192 1132
Size: 97 852 351 89 117 1009 1545 2229
Fragments arranged by size:
2229 1545 1009 852 351 117 97 89
PasI CC'CwG_GG
Cuts at: 1348 2137 1348
Size: 789 5500
PciI A'CATG_T
Cuts at: 5188 5188
Size: 6289
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3117 3343 3370 1108
Size: 2009 226 27 4027
Fragments arranged by size:
4027 2009 226 27
PfoI T'CCnGG_A
Cuts at: 2373 2373
Size: 6289
PmeI GTTT'AAAC
Cuts at: 3593 3593
Size: 6289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 5871 5903 4358
Size: 32 1481 32 4744
Fragments arranged by size:
4744 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2150 2203 3514 2150
Size: 53 1311 4925
Fragments arranged by size:
4925 1311 53
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6289
PsiI TTA'TAA
Cuts at: 3859 3859
Size: 6289
PspOMI G'GGCC_C
Cuts at: 2115 3428 2115
Size: 1313 4976
PstI C_TGCA'G
Cuts at: 830 2664 3411 3641 830
Size: 1834 747 230 3478
Fragments arranged by size:
3478 1834 747 230
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6289
PvuII CAG'CTG
Cuts at: 3310 3449 3310
Size: 139 6150
SacI G_AGCT'C
Cuts at: 3189 3189
Size: 6289
SacII CC_GC'GG
Cuts at: 2820 3040 2820
Size: 220 6069
SalI G'TCGA_C
Cuts at: 1936 3631 1936
Size: 1695 4594
SanDI GG'GwC_CC
Cuts at: 3514 3514
Size: 6289
SbfI CC_TGCA'GG
Cuts at: 3641 3641
Size: 6289
ScaI AGT'ACT
Cuts at: 4537 6249 4537
Size: 1712 4577
SexAI A'CCwGG_T
Cuts at: 2457 2457
Size: 6289
SfcI C'TryA_G
Cuts at: 826 1045 2660 3407 3637 4772 5453 5644 826
Size: 219 1615 747 230 1135 681 191 1471
Fragments arranged by size:
1615 1471 1135 747 681 230 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2615 2615
Size: 6289
SfoI GGC'GCC
Cuts at: 2485 2485
Size: 6289
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6289
SmaI CCC'GGG
Cuts at: 1872 2615 3616 1872
Size: 743 1001 4545
Fragments arranged by size:
4545 1001 743
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3527 4393 5294 5556 820
Size: 197 926 1584 866 901 262 1553
Fragments arranged by size:
1584 1553 926 901 866 262 197
SnaBI TAC'GTA
Cuts at: 493 4080 493
Size: 3587 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6289
SphI G_CATG'C
Cuts at: 1132 2081 2432 2638 3647 1132
Size: 949 351 206 1009 3774
Fragments arranged by size:
3774 1009 949 351 206
SrfI GCCC'GGGC
Cuts at: 2615 2615
Size: 6289
SspI AAT'ATT
Cuts at: 5 52 4213 5
Size: 47 4161 2081
Fragments arranged by size:
4161 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6289
StyI C'CwwG_G
Cuts at: 513 1065 2004 2040 2185 2275 2571 3728 513
Size: 552 939 36 145 90 296 1157 3074
Fragments arranged by size:
3074 1157 939 552 296 145 90 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2606 4316 4333 4486 4671 5116 325
Size: 1255 1026 1710 17 153 185 445 1498
Fragments arranged by size:
1710 1498 1255 1026 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4535 6247 96
Size: 274 80 33 51 1443 2558 1712 138
Fragments arranged by size:
2558 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6249 6281 6249
Size: 32 6257
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6289
XbaI T'CTAG_A
Cuts at: 3625 3625
Size: 6289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3491 3491
Size: 6289
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6289
XmaI C'CCGG_G
Cuts at: 1870 2613 3614 1870
Size: 743 1001 4545
Fragments arranged by size:
4545 1001 743
XmnI GAAnn'nnTTC
Cuts at: 4418 4418
Size: 6289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4097 276
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SapI SwaI