(Linear) (Six-base) MAPSORT of: KIEE1964.seq Check: 8887 from: 1 to: 1584 KIEE1964.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 87 445 455 1584 Size: 87 358 10 1129 Fragments arranged by size: 1129 358 87 10 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 211 970 1396 1479 1584 Size: 88 123 759 426 83 105 Fragments arranged by size: 759 426 123 105 88 83 AfeI AGC'GCT Cuts at: 0 777 994 1584 Size: 777 217 590 Fragments arranged by size: 777 590 217 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 865 897 1584 Size: 865 32 687 Fragments arranged by size: 865 687 32 AlwNI CAG_nnn'CTG Cuts at: 0 369 546 1584 Size: 369 177 1038 Fragments arranged by size: 1038 369 177 ApaI G_GGCC'C Cuts at: 0 114 1427 1584 Size: 114 1313 157 Fragments arranged by size: 1313 157 114 AscI GG'CGCG_CC Cuts at: 0 87 1584 Size: 87 1497 AvaI C'yCGr_G Cuts at: 0 608 1147 1426 1584 Size: 608 539 279 158 Fragments arranged by size: 608 539 279 158 BanI G'GyrC_C Cuts at: 0 478 831 1584 Size: 478 353 753 Fragments arranged by size: 753 478 353 BanII G_rGCy'C Cuts at: 0 114 188 1184 1252 1312 1427 1435 1584 Size: 114 74 996 68 60 115 8 149 Fragments arranged by size: 996 149 115 114 74 68 60 8 BbeI G_GCGC'C Cuts at: 0 482 1584 Size: 482 1102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 724 758 1584 Size: 724 34 826 Fragments arranged by size: 826 724 34 BglI GCCn_nnn'nGGC Cuts at: 0 610 1102 1172 1584 Size: 610 492 70 412 Fragments arranged by size: 610 492 412 70 BlpI GC'TnA_GC Cuts at: 0 1306 1445 1584 Size: 1306 139 139 Fragments arranged by size: 1306 139 139 Bme1580I G_kGCm'C Cuts at: 0 114 836 1427 1584 Size: 114 722 591 157 Fragments arranged by size: 722 591 157 114 BmgBI CAC'GTC Cuts at: 0 711 1584 Size: 711 873 BmrI ACTGGGnnnn_n' Cuts at: 0 206 831 1249 1584 Size: 206 625 418 335 Fragments arranged by size: 625 418 335 206 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 247 391 577 964 1002 1584 Size: 34 213 144 186 387 38 582 Fragments arranged by size: 582 387 213 186 144 38 34 Bpu10I CC'TnA_GC Cuts at: 0 772 779 1183 1584 Size: 772 7 404 401 Fragments arranged by size: 772 404 401 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1507 1584 Size: 1507 77 BsaHI Gr'CG_yC Cuts at: 0 415 479 898 965 1584 Size: 415 64 419 67 619 Fragments arranged by size: 619 419 415 67 64 BsaWI w'CCGG_w Cuts at: 0 644 1286 1584 Size: 644 642 298 Fragments arranged by size: 644 642 298 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 937 1216 1575 1584 Size: 937 279 359 9 Fragments arranged by size: 937 359 279 9 BseYI C'CCAG_C Cuts at: 0 92 376 794 1064 1478 1584 Size: 92 284 418 270 414 106 Fragments arranged by size: 418 414 284 270 106 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 379 433 691 790 1584 Size: 307 72 54 258 99 794 Fragments arranged by size: 794 307 258 99 72 54 BsiEI CG_ry'CG Cuts at: 0 576 1584 Size: 576 1008 BsiHKAI G_wGCw'C Cuts at: 0 11 55 1184 1584 Size: 11 44 1129 400 Fragments arranged by size: 1129 400 44 11 BsmBI CGTCTCn'nnnn_ Cuts at: 0 889 1584 Size: 889 695 Bsp1286I G_dGCh'C Cuts at: 0 11 55 114 188 836 1184 1252 1312 Size: 11 44 59 74 648 348 68 60 Cuts at: 1312 1427 1435 1584 Size: 115 8 149 Fragments arranged by size: 648 348 149 115 74 68 60 59 44 11 8 BspEI T'CCGG_A Cuts at: 0 644 1584 Size: 644 940 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 87 186 445 455 1584 Size: 87 99 259 10 1129 Fragments arranged by size: 1129 259 99 87 10 BsrBI CCG'CTC Cuts at: 0 63 1584 Size: 63 1521 BsrFI r'CCGG_y Cuts at: 0 650 1024 1584 Size: 650 374 560 Fragments arranged by size: 650 560 374 BssHII G'CGCG_C Cuts at: 0 87 975 1584 Size: 87 888 609 Fragments arranged by size: 888 609 87 BssSI C'ACGA_G Cuts at: 0 850 1584 Size: 850 734 BstEII G'GTnAC_C Cuts at: 0 1470 1584 Size: 1470 114 BstXI CCAn_nnnn'nTGG Cuts at: 0 271 489 942 1584 Size: 271 218 453 642 Fragments arranged by size: 642 453 271 218 Bsu36I CC'TnA_GG Cuts at: 0 1418 1584 Size: 1418 166 BtgI C'CryG_G Cuts at: 0 35 566 576 690 812 1032 1553 1584 Size: 35 531 10 114 122 220 521 31 Fragments arranged by size: 531 521 220 122 114 35 31 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 453 1584 Size: 453 1131 DraIII CAC_nnn'GTG Cuts at: 0 456 1584 Size: 456 1128 DrdI GACnn_nn'nnGTC Cuts at: 0 1291 1584 Size: 1291 293 EaeI y'GGCC_r Cuts at: 0 2 296 431 441 569 1236 1584 Size: 2 294 135 10 128 667 348 Fragments arranged by size: 667 348 294 135 128 10 2 EarI CTCTTCn'nnn_ Cuts at: 0 208 1584 Size: 208 1376 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 250 1584 Size: 250 1334 EcoICRI GAG'CTC Cuts at: 0 1182 1584 Size: 1182 402 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 88 211 247 391 577 964 970 Size: 34 54 123 36 144 186 387 6 Cuts at: 970 1002 1396 1479 1584 Size: 32 394 83 105 Fragments arranged by size: 394 387 186 144 123 105 83 54 36 34 32 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 193 226 1584 Size: 193 33 1358 Fragments arranged by size: 1358 193 33 EcoO109I rG'GnC_Cy Cuts at: 0 145 198 1423 1509 1584 Size: 145 53 1225 86 75 Fragments arranged by size: 1225 145 86 75 53 FspI TGC'GCA Cuts at: 0 628 1584 Size: 628 956 HaeII r_GCGC'y Cuts at: 0 482 779 996 1025 1383 1584 Size: 482 297 217 29 358 201 Fragments arranged by size: 482 358 297 217 201 29 HincII GTy'rAC Cuts at: 0 498 1584 Size: 498 1086 KasI G'GCGC_C Cuts at: 0 478 1584 Size: 478 1106 MscI TGG'CCA Cuts at: 0 298 1584 Size: 298 1286 MspA1I CmG'CkG Cuts at: 0 235 804 814 1034 1305 1444 1584 Size: 235 569 10 220 271 139 140 Fragments arranged by size: 569 271 235 220 140 139 10 NaeI GCC'GGC Cuts at: 0 652 1026 1584 Size: 652 374 558 Fragments arranged by size: 652 558 374 NarI GG'CG_CC Cuts at: 0 479 1584 Size: 479 1105 NcoI C'CATG_G Cuts at: 0 35 566 1584 Size: 35 531 1018 Fragments arranged by size: 1018 531 35 NgoMIV G'CCGG_C Cuts at: 0 650 1024 1584 Size: 650 374 560 Fragments arranged by size: 650 560 374 NspI r_CATG'y Cuts at: 0 76 427 516 633 1584 Size: 76 351 89 117 951 Fragments arranged by size: 951 351 117 89 76 PasI CC'CwG_GG Cuts at: 0 132 1584 Size: 132 1452 PflMI CCAn_nnn'nTGG Cuts at: 0 1112 1338 1365 1584 Size: 1112 226 27 219 Fragments arranged by size: 1112 226 219 27 PfoI T'CCnGG_A Cuts at: 0 368 1584 Size: 368 1216 PpuMI rG'GwC_Cy Cuts at: 0 145 198 1509 1584 Size: 145 53 1311 75 Fragments arranged by size: 1311 145 75 53 PspOMI G'GGCC_C Cuts at: 0 110 1423 1584 Size: 110 1313 161 Fragments arranged by size: 1313 161 110 PstI C_TGCA'G Cuts at: 0 659 1406 1584 Size: 659 747 178 Fragments arranged by size: 747 659 178 PvuII CAG'CTG Cuts at: 0 1305 1444 1584 Size: 1305 139 140 Fragments arranged by size: 1305 140 139 SacI G_AGCT'C Cuts at: 0 1184 1584 Size: 1184 400 SacII CC_GC'GG Cuts at: 0 815 1035 1584 Size: 815 220 549 Fragments arranged by size: 815 549 220 SanDI GG'GwC_CC Cuts at: 0 1509 1584 Size: 1509 75 SexAI A'CCwGG_T Cuts at: 0 452 1584 Size: 452 1132 SfcI C'TryA_G Cuts at: 0 655 1402 1584 Size: 655 747 182 Fragments arranged by size: 747 655 182 SfiI GGCCn_nnn'nGGCC Cuts at: 0 610 1584 Size: 610 974 SfoI GGC'GCC Cuts at: 0 480 1584 Size: 480 1104 SmaI CCC'GGG Cuts at: 0 610 1584 Size: 610 974 SmlI C'TyrA_G Cuts at: 0 1522 1584 Size: 1522 62 SphI G_CATG'C Cuts at: 0 76 427 633 1584 Size: 76 351 206 951 Fragments arranged by size: 951 351 206 76 SrfI GCCC'GGGC Cuts at: 0 610 1584 Size: 610 974 StyI C'CwwG_G Cuts at: 0 35 180 270 566 1584 Size: 35 145 90 296 1018 Fragments arranged by size: 1018 296 145 90 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 601 1584 Size: 601 983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1486 1584 Size: 1486 98 XmaI C'CCGG_G Cuts at: 0 608 1584 Size: 608 976 Enzymes that do cut: AarI AcuI AfeI AloI AlwNI ApaI AscI AvaI BanI BanII BbeI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BssSI BstEII BstXI Bsu36I BtgI BtgZI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII HincII KasI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SexAI SfcI SfiI SfoI SmaI SmlI SphI SrfI StyI TaqII XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BmtI BplI BsaI BsaAI BsaBI BsaXI BsiWI BsmI BspHI BsrDI BsrGI BstAPI BstBI BstYI BstZ17I BtsI ClaI CspCI DraI EagI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI MslI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SgfI SgrAI SnaBI SpeI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI