################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvrII BalI BamHI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsgI BsiWI BsmI BspDI BspHI BsrBI BsrDI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoRI FbaI FseI KpnI MaeI MamI MfeI MluI MluNI MscI MunI NheI NotI NruI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 73 enzymes) # ################################### AccIII 5 TCCGGA T^CCGGA AcsI 429 AAATTT [GA]^AATT[CT] AocI 1171 CCTCAGG CC^T[ACGT]AGG ApoI 429 AAATTT [GA]^AATT[CT] Asp700I 1243 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1362 GAGCAC G[AT]GC[AT]^C AspI 1032 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 595 CTCGAG C^[CT]CG[GA]G AviII 698 TGCGCA TGC^GCA BbeI 196 GGCGCC GGCGC^C BbsI 1282 GTCTTC GAAGAC(2/6) BbvCI 608 CCTCAGC CCTCAGC(-5/-2) BglI 647 GCCTCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 108 GCTCAGC GC^T[ACGT]AGC Bpu1102I 108 GCTCAGC GC^T[ACGT]AGC BpuAI 1282 GTCTTC GAAGAC(2/6) BsaI 184 GAGACC GGTCTC(1/5) BsaWI 5 TCCGGA [AT]^CCGG[AT] BseAI 5 TCCGGA T^CCGGA BsiEI 57 CGGCCG CG[GA][CT]^CG BsiHKAI 1362 GAGCAC G[AT]GC[AT]^C BsoBI 595 CTCGAG C^[CT]CG[GA]G Bsp1407I 794 TGTACA T^GTACA BspEI 5 TCCGGA T^CCGGA BspLU11I 791 ACATGT A^CATGT BsrGI 794 TGTACA T^GTACA BstAPI 1364 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1422 GGTGACC G^GT[ACGT]ACC BstPI 1422 GGTGACC G^GT[ACGT]ACC BstXI 299 CCAAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1120 GGATCT [GA]^GATC[CT] Bsu36I 1171 CCTCAGG CC^T[ACGT]AGG CelII 108 GCTCAGC GC^T[ACGT]AGC DrdI 9 GACCCCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 57 CGGCCG C^GGCCG EclXI 57 CGGCCG C^GGCCG Eco52I 57 CGGCCG C^GGCCG Eco81I 1171 CCTCAGG CC^T[ACGT]AGG EcoO65I 1422 GGTGACC G^GT[ACGT]ACC EcoRV 991 GATATC GAT^ATC EcoT22I 881 ATGCAT ATGCA^T FspI 698 TGCGCA TGC^GCA HgaI 129 GCGTC GACGC(5/10) HincII 418 GTTAAC GT[CT]^[GA]AC HindII 418 GTTAAC GT[CT]^[GA]AC HindIII 819 AAGCTT A^AGCTT HinfI 1224 GAGTC G^A[ACGT]TC HpaI 418 GTTAAC GTT^AAC KasI 196 GGCGCC G^GCGCC KspI 54 CCGCGG CCGC^GG MflI 1120 GGATCT [GA]^GATC[CT] MroI 5 TCCGGA T^CCGGA MseI 419 TTAA T^TAA NaeI 113 GCCGGC GCC^GGC NarI 196 GGCGCC GG^CGCC NdeI 1349 CATATG CA^TATG NgoMIV 113 GCCGGC G^CCGGC NsiI 881 ATGCAT ATGCA^T PaeR7I 595 CTCGAG C^TCGAG PflFI 1032 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1224 GAGTC GAGTC(4/5) SacII 54 CCGCGG CCGC^GG SapI 1243 GAAGAGC GCTCTTC(1/4) SfoI 196 GGCGCC GGC^GCC SmlI 595 CTCGAG C^T[CT][GA]AG SspBI 794 TGTACA T^GTACA TaqI 596 TCGA T^CGA Tru9I 419 TTAA T^TAA Tth111I 1032 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 596 TCGA T^CGA XhoI 595 CTCGAG C^TCGAG XhoII 1120 GGATCT [GA]^GATC[CT] XmnI 1243 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 128 GGCGTC G[GA]^CG[CT]C 196 GGCGCC AfaI 752 GTAC GT^AC 795 GTAC AflIII 791 ACATGT A^C[GA][CT]GT 1073 ACGTGT AlwI 3 GATCC GGATC(4/5) 1120 GGATC BcnI 193 CCGGG CC^[GC]GG 247 CCGGG BsaHI 128 GGCGTC G[GA]^CG[CT]C 196 GGCGCC BsmBI 504 CGTCTC CGTCTC(1/5) 467 GAGACG BsrFI 113 GCCGGC [GA]^CCGG[CT] 1163 GCCGGT BssHII 104 GCGCGC G^CGCGC 323 GCGCGC Cfr10I 113 GCCGGC [GA]^CCGG[CT] 1163 GCCGGT DraIII 1381 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG 1521 CACCCAGTG EarI 235 CTCTTC CTCTTC(1/4) 1243 GAAGAG EcoNI 372 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1206 CCTGATAGAGG HaeII 196 GGCGCC [GA]GCGC^[CT] 259 AGCGCC Hin1I 128 GGCGTC G[GA]^CG[CT]C 196 GGCGCC Ksp632I 235 CTCTTC CTCTTC(1/4) 1243 GAAGAG MaeII 803 ACGT A^CGT 1073 ACGT NciI 193 CCGGG CC^[GC]GG 247 CCGGG PvuII 611 CAGCTG CAG^CTG 947 CAGCTG RsaI 752 GTAC GT^AC 795 GTAC StuI 276 AGGCCT AGG^CCT 1488 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccII AciI AluI AlwNI AvaII BanI BanII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NspI PpuMI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I Tsp509I TspRI