(Circular) (Six-base) MAPSORT of: pFN21AE1957.seq Check: 4234 from: 1 to: 6241 pFN21AE1957 6241 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2759 2759 Size: 6241 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4051 278 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3584 1937 Size: 1647 4594 Acc65I G'GTAC_C Cuts at: 3562 3562 Size: 6241 AclI AA'CG_TT Cuts at: 1563 4368 4741 1563 Size: 2805 373 3063 Fragments arranged by size: 3063 2805 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 2640 3032 3274 4304 5688 5956 1632 Size: 767 241 392 242 1030 1384 268 1917 Fragments arranged by size: 1917 1384 1030 767 392 268 242 241 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6044 AflIII A'CryG_T Cuts at: 1203 2796 3078 5140 6117 1203 Size: 1593 282 2062 977 1327 Fragments arranged by size: 2062 1593 1327 977 282 AgeI A'CCGG_T Cuts at: 5072 5072 Size: 6241 AhdI GACnn_n'nnGTC Cuts at: 4970 4970 Size: 6241 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6241 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6241 AlwNI CAG_nnn'CTG Cuts at: 2338 2389 3010 3184 3984 5556 2338 Size: 51 621 174 800 1572 3023 Fragments arranged by size: 3023 1572 800 621 174 51 ApaLI G'TGCA_C Cuts at: 4298 5454 4298 Size: 1156 5085 ApoI r'AATT_y Cuts at: 1493 2434 3550 3776 3928 6037 1493 Size: 941 1116 226 152 2109 1697 Fragments arranged by size: 2109 1697 1116 941 226 152 AseI AT'TA_AT Cuts at: 160 1032 4795 160 Size: 872 3763 1606 Fragments arranged by size: 3763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 AvaI C'yCGr_G Cuts at: 1870 1943 2600 3566 1870 Size: 73 657 966 4545 Fragments arranged by size: 4545 966 657 73 BamHI G'GATC_C Cuts at: 3571 3938 3571 Size: 367 5874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2201 2297 2486 3562 5017 Size: 325 217 627 414 96 189 1076 1455 Cuts at: 5017 618 Size: 1842 Fragments arranged by size: 1842 1455 1076 627 414 325 217 189 96 BanII G_rGCy'C Cuts at: 1391 2215 2231 3335 3560 1391 Size: 824 16 1104 225 4072 Fragments arranged by size: 4072 1104 824 225 16 BbeI G_GCGC'C Cuts at: 2205 2205 Size: 6241 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3280 928 Size: 971 1381 3889 Fragments arranged by size: 3889 1381 971 BbvCI CC'TCA_GC Cuts at: 2614 2614 Size: 6241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4432 4466 1903 Size: 34 2495 34 3678 Fragments arranged by size: 3678 2495 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4133 5343 4133 Size: 1210 5031 BclI T'GATC_A Cuts at: 1548 1548 Size: 6241 BfrBI ATG'CAT Cuts at: 2888 2888 Size: 6241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2658 4852 136 Size: 107 122 71 631 605 986 2194 1525 Fragments arranged by size: 2194 1525 986 631 605 122 107 71 BglII A'GATC_T Cuts at: 6236 6236 Size: 6241 BlpI GC'TnA_GC Cuts at: 2114 3658 2114 Size: 1544 4697 Bme1580I G_kGCm'C Cuts at: 1790 2491 4302 5458 1790 Size: 701 1811 1156 2573 Fragments arranged by size: 2573 1811 1156 701 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2485 3200 3523 Size: 416 53 83 399 24 1068 715 323 Cuts at: 3523 3972 4930 442 Size: 449 958 1753 Fragments arranged by size: 1753 1068 958 715 449 416 399 323 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6241 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2495 2495 Size: 6241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2573 3206 3259 3443 3478 4901 1239 Size: 1334 633 53 184 35 1423 2579 Fragments arranged by size: 2579 1423 1334 633 184 53 35 Bpu10I CC'TnA_GC Cuts at: 2614 2614 Size: 6241 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4366 5231 5529 4366 Size: 865 298 5078 Fragments arranged by size: 5078 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2183 4904 882 Size: 617 684 2721 2219 Fragments arranged by size: 2721 2219 684 617 BsaAI yAC'GTr Cuts at: 493 1204 4032 493 Size: 711 2828 2702 Fragments arranged by size: 2828 2702 711 BsaBI GATnn'nnATC Cuts at: 6235 6235 Size: 6241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2134 2202 4048 4430 Size: 53 83 186 936 601 68 1846 382 Cuts at: 4430 5065 275 Size: 635 1451 Fragments arranged by size: 1846 1451 936 635 601 382 186 83 68 53 BsaWI w'CCGG_w Cuts at: 2010 4674 5072 5346 5493 5904 2010 Size: 2664 398 274 147 411 2347 Fragments arranged by size: 2664 2347 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3179 3209 3332 3362 1178 Size: 30 165 30 1776 30 123 30 4057 Fragments arranged by size: 4057 1776 165 123 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2861 3233 3329 1186 Size: 850 825 372 96 4098 Fragments arranged by size: 4098 850 825 372 96 BseYI C'CCAG_C Cuts at: 2224 2234 2261 2719 5444 5940 2224 Size: 10 27 458 2725 496 2525 Fragments arranged by size: 2725 2525 496 458 27 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5668 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2065 3691 4452 5480 1540 Size: 275 186 64 1626 761 1028 2301 Fragments arranged by size: 2301 1626 1028 761 275 186 64 BsiHKAI G_wGCw'C Cuts at: 3371 4302 4387 5458 3371 Size: 931 85 1071 4154 Fragments arranged by size: 4154 1071 931 85 BsmI GAATG_Cn' Cuts at: 3752 3845 3752 Size: 93 6148 BsmBI CGTCTCn'nnnn_ Cuts at: 2466 2515 2466 Size: 49 6192 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2215 2231 2491 3335 3371 3560 4302 Size: 399 425 16 260 844 36 189 742 Cuts at: 4302 4387 5458 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 844 742 425 399 260 189 85 36 16 BspEI T'CCGG_A Cuts at: 2010 5904 2010 Size: 3894 2347 BspHI T'CATG_A Cuts at: 4130 5860 4130 Size: 1730 4511 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2385 2436 2759 3075 3285 3596 844 Size: 1541 51 323 316 210 311 3489 Fragments arranged by size: 3489 1541 323 316 311 210 51 BsrBI CCG'CTC Cuts at: 1394 1823 4128 1394 Size: 429 2305 3507 Fragments arranged by size: 3507 2305 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4736 4910 65 Size: 1183 3488 174 1396 Fragments arranged by size: 3488 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2118 3168 4885 5072 1700 Size: 110 308 1050 1717 187 2869 Fragments arranged by size: 2869 1717 1050 308 187 110 BsrGI T'GTAC_A Cuts at: 96 2799 96 Size: 2703 3538 BssHII G'CGCG_C Cuts at: 1925 2109 2328 1925 Size: 184 219 5838 Fragments arranged by size: 5838 219 184 BssSI C'ACGA_G Cuts at: 2357 2480 3389 4301 5313 2357 Size: 123 909 912 1012 3285 Fragments arranged by size: 3285 1012 912 909 123 BstAPI GCAn_nnn'nTGC Cuts at: 794 3375 794 Size: 2581 3660 BstBI TT'CG_AA Cuts at: 5045 5045 Size: 6241 BstEII G'GTnAC_C Cuts at: 1184 3427 1184 Size: 2243 3998 BstXI CCAn_nnnn'nTGG Cuts at: 2311 2311 Size: 6241 BstYI r'GATC_y Cuts at: 1972 3125 3571 3938 4323 4340 5792 5878 5890 Size: 1153 446 367 385 17 1452 86 12 Cuts at: 5890 6236 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1153 446 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3177 3177 Size: 6241 BtgI C'CryG_G Cuts at: 513 1065 2059 2088 2443 2762 3045 3336 513 Size: 552 994 29 355 319 283 291 3418 Fragments arranged by size: 3418 994 552 355 319 291 283 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2021 487 Size: 683 56 795 4707 Fragments arranged by size: 4707 795 683 56 BtsI GCAGTG_nn' Cuts at: 3065 3757 4551 4571 3065 Size: 692 794 20 4735 Fragments arranged by size: 4735 794 692 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6206 DraI TTT'AAA Cuts at: 3545 3545 Size: 6241 DraIII CAC_nnn'GTG Cuts at: 3391 3531 3391 Size: 140 6101 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2020 5248 809 Size: 507 704 3228 1802 Fragments arranged by size: 3228 1802 704 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 2365 3166 Size: 54 1425 50 275 18 232 303 801 Cuts at: 3166 3688 4577 6206 8 Size: 522 889 1629 43 Fragments arranged by size: 1629 1425 889 801 522 303 275 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2062 3688 1537 Size: 275 250 1626 4090 Fragments arranged by size: 4090 1626 275 250 EarI CTCTTCn'nnn_ Cuts at: 1353 2246 3243 4171 1353 Size: 893 997 928 3423 Fragments arranged by size: 3423 997 928 893 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4828 5202 5348 387 Size: 1064 138 3239 374 146 1280 Fragments arranged by size: 3239 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 2573 2640 3032 3206 3259 3274 Size: 393 767 174 67 392 174 53 15 Cuts at: 3274 3443 3478 4304 4901 5688 5956 1239 Size: 169 35 826 597 787 268 1524 Fragments arranged by size: 1524 826 787 767 597 393 392 268 174 174 169 67 53 35 15 EcoNI CCTnn'n_nnAGG Cuts at: 2381 3215 2381 Size: 834 5407 EcoO109I rG'GnC_Cy Cuts at: 2335 2386 2911 3451 3462 2335 Size: 51 525 540 11 5114 Fragments arranged by size: 5114 540 525 51 11 EcoRI G'AATT_C Cuts at: 3550 3928 3550 Size: 378 5863 EcoRV GAT'ATC Cuts at: 2998 2998 Size: 6241 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3281 3281 Size: 6241 FspI TGC'GCA Cuts at: 2705 4747 2705 Size: 2042 4199 HaeII r_GCGC'y Cuts at: 1678 2205 2268 5388 1678 Size: 527 63 3120 2531 Fragments arranged by size: 3120 2531 527 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3179 3211 4882 4914 4956 Size: 32 135 32 1607 32 1671 32 42 Cuts at: 4956 4988 1373 Size: 32 2626 Fragments arranged by size: 2626 1671 1607 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2425 3585 3831 669 Size: 988 281 487 1160 246 3079 Fragments arranged by size: 3079 1160 988 487 281 246 HindIII A'AGCT_T Cuts at: 748 2824 748 Size: 2076 4165 HpaI GTT'AAC Cuts at: 2425 3831 2425 Size: 1406 4835 KasI G'GCGC_C Cuts at: 2201 2201 Size: 6241 KpnI G_GTAC'C Cuts at: 3566 3566 Size: 6241 MfeI C'AATT_G Cuts at: 3840 3840 Size: 6241 MluI A'CGCG_T Cuts at: 6117 6117 Size: 6241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5355 5539 1305 Size: 4050 184 2007 Fragments arranged by size: 4050 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4419 Fragments arranged by size: 4419 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2660 3029 3050 4199 4558 4717 518 Size: 648 1494 369 21 1149 359 159 2042 Fragments arranged by size: 2042 1494 1149 648 369 359 159 21 MspA1I CmG'CkG Cuts at: 1606 2061 2618 2814 2954 3657 4334 5482 5727 Size: 455 557 196 140 703 677 1148 245 Cuts at: 5727 1606 Size: 2120 Fragments arranged by size: 2120 1148 703 677 557 455 245 196 140 NaeI GCC'GGC Cuts at: 2120 2120 Size: 6241 NarI GG'CG_CC Cuts at: 2202 2202 Size: 6241 NcoI C'CATG_G Cuts at: 513 1065 2443 2762 3045 513 Size: 552 1378 319 283 3709 Fragments arranged by size: 3709 1378 552 319 283 NdeI CA'TA_TG Cuts at: 387 3355 387 Size: 2968 3273 NgoMIV G'CCGG_C Cuts at: 2118 2118 Size: 6241 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6241 NotI GC'GGCC_GC Cuts at: 3688 3688 Size: 6241 NsiI A_TGCA'T Cuts at: 2890 2890 Size: 6241 NspI r_CATG'y Cuts at: 1132 1229 2083 2581 2800 3599 5144 1132 Size: 97 854 498 219 799 1545 2229 Fragments arranged by size: 2229 1545 854 799 498 219 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6241 PciI A'CATG_T Cuts at: 2796 5140 2796 Size: 2344 3897 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6241 PfoI T'CCnGG_A Cuts at: 2913 2913 Size: 6241 PmeI GTTT'AAAC Cuts at: 3545 3545 Size: 6241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4310 4342 5823 5855 4310 Size: 32 1481 32 4696 Fragments arranged by size: 4696 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2386 2911 3451 3462 2386 Size: 525 540 11 5165 Fragments arranged by size: 5165 540 525 11 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6241 PsiI TTA'TAA Cuts at: 3811 3811 Size: 6241 PspXI vC'TCGA_Gb Cuts at: 2600 2600 Size: 6241 PstI C_TGCA'G Cuts at: 830 2756 3006 3060 3593 830 Size: 1926 250 54 533 3478 Fragments arranged by size: 3478 1926 533 250 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6241 PvuII CAG'CTG Cuts at: 2618 2954 2618 Size: 336 5905 SacII CC_GC'GG Cuts at: 2062 2062 Size: 6241 SalI G'TCGA_C Cuts at: 1936 3583 1936 Size: 1647 4594 SanDI GG'GwC_CC Cuts at: 3451 3451 Size: 6241 SapI GCTCTTCn'nnn_ Cuts at: 3243 3243 Size: 6241 SbfI CC_TGCA'GG Cuts at: 3593 3593 Size: 6241 ScaI AGT'ACT Cuts at: 4489 6201 4489 Size: 1712 4529 SfcI C'TryA_G Cuts at: 826 1045 2752 3002 3056 3589 4724 5405 5596 Size: 219 1707 250 54 533 1135 681 191 Cuts at: 5596 826 Size: 1471 Fragments arranged by size: 1707 1471 1135 681 533 250 219 191 54 SfoI GGC'GCC Cuts at: 2203 2203 Size: 6241 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6241 SmaI CCC'GGG Cuts at: 1872 3568 1872 Size: 1696 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2600 4345 5246 5508 820 Size: 197 926 657 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 657 262 197 SnaBI TAC'GTA Cuts at: 493 4032 493 Size: 3539 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6241 SphI G_CATG'C Cuts at: 1132 3599 1132 Size: 2467 3774 SspI AAT'ATT Cuts at: 5 52 4165 5 Size: 47 4113 2081 Fragments arranged by size: 4113 2081 47 StuI AGG'CCT Cuts at: 1517 2283 3495 1517 Size: 766 1212 4263 Fragments arranged by size: 4263 1212 766 StyI C'CwwG_G Cuts at: 513 1065 2443 2762 3045 3680 513 Size: 552 1378 319 283 635 3074 Fragments arranged by size: 3074 1378 635 552 319 283 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2474 3542 4268 4285 4438 4623 5068 Size: 1255 894 1068 726 17 153 185 445 Cuts at: 5068 325 Size: 1498 Fragments arranged by size: 1498 1255 1068 894 726 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2756 2799 4487 Size: 274 80 33 51 1443 779 43 1688 Cuts at: 4487 6199 96 Size: 1712 138 Fragments arranged by size: 1712 1688 1443 779 274 138 80 51 43 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6201 6233 6201 Size: 32 6209 Tth111I GACn'n_nGTC Cuts at: 1316 3040 1316 Size: 1724 4517 XbaI T'CTAG_A Cuts at: 3577 3577 Size: 6241 XhoI C'TCGA_G Cuts at: 1943 2600 1943 Size: 657 5584 XmaI C'CCGG_G Cuts at: 1870 3566 1870 Size: 1696 4545 XmnI GAAnn'nnTTC Cuts at: 3252 4370 3252 Size: 1118 5123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4049 276 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I EcoICRI FseI FspAI NruI PacI PmlI PspOMI PsrI RsrII SacI SexAI SfiI SrfI SwaI XcmI