################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII AccII AccIII AciI AclI AcsI AcyI AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI Asp700I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstUI CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 984 GGTACC G^GTACC AccI 463 GTATAC GT^[AC][GT]AC AflII 669 CTTAAG C^TTAAG AflIII 347 ACATGT A^C[GA][CT]GT AlwI 194 GGATC GGATC(4/5) AocI 938 CCTCAGG CC^T[ACGT]AGG AseI 289 ATTAAT AT^TAAT AsnI 289 ATTAAT AT^TAAT Asp718I 984 GGTACC G^GTACC AspHI 1185 GAGCAC G[AT]GC[AT]^C AvaII 425 GGTCC G^G[AT]CC BalI 172 TGGCCA TGG^CCA BanI 984 GGTACC G^G[CT][GA]CC BfrI 669 CTTAAG C^TTAAG BglII 837 AGATCT A^GATCT BmyI 1185 GAGCAC G[AGT]GC[ACT]^C BsaJI 107 CCCAGG C^C[ACGT][ACGT]GG BseRI 130 GAGGAG GAGGAG(10/8) BsiHKAI 1185 GAGCAC G[AT]GC[AT]^C BsiYI 89 CCAAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 89 CCAAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 399 GCATTC GAATGC(1/-1) Bsp1286I 1185 GAGCAC G[AGT]GC[ACT]^C BspHI 375 TCATGA T^CATGA BspLU11I 347 ACATGT A^CATGT Bst1107I 463 GTATAC GTA^TAC BstEII 135 GGTCACC G^GT[ACGT]ACC BstPI 135 GGTCACC G^GT[ACGT]ACC BstXI 917 CCATTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 837 AGATCT [GA]^GATC[CT] BstZ17I 463 GTATAC GTA^TAC Bsu36I 938 CCTCAGG CC^T[ACGT]AGG Cac8I 649 GCTTGC GC[ACGT]^[ACGT]GC Cfr13I 425 GGTCC G^G[ACGT]CC EaeI 172 TGGCCA [CT]^GGCC[GA] Eco81I 938 CCTCAGG CC^T[ACGT]AGG EcoO65I 135 GGTCACC G^GT[ACGT]ACC EcoRV 664 GATATC GAT^ATC HincII 522 GTTGAC GT[CT]^[GA]AC HindII 522 GTTGAC GT[CT]^[GA]AC KpnI 984 GGTACC GGTAC^C MaeII 635 ACGT A^CGT MflI 837 AGATCT [GA]^GATC[CT] MluNI 172 TGGCCA TGG^CCA MscI 172 TGGCCA TGG^CCA NspI 347 ACATGT [GA]CATG^[CT] PshAI 1139 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 289 ATTAAT AT^TAAT RcaI 375 TCATGA T^CATGA SapI 1182 GAAGAGC GCTCTTC(1/4) Sau96I 425 GGTCC G^G[ACGT]CC ScaI 1009 AGTACT AGT^ACT SpeI 1012 ACTAGT A^CTAGT XbaI 435 TCTAGA T^CTAGA XhoII 837 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BbsI 1029 GAAGAC GAAGAC(2/6) 1054 GAAGAC BlpI 28 GCTAAGC GC^T[ACGT]AGC 826 GCTCAGC BpmI 252 CTCCAG CTGGAG(16/14) 1099 CTGGAG Bpu1102I 28 GCTAAGC GC^T[ACGT]AGC 826 GCTCAGC BpuAI 1029 GAAGAC GAAGAC(2/6) 1054 GAAGAC BsmFI 953 GGGAC GGGAC(10/14) 1137 GGGAC BsrDI 582 CATTGC GCAATG(2/0) 1157 GCAATG CelII 28 GCTAAGC GC^T[ACGT]AGC 826 GCTCAGC DpnII 195 GATC ^GATC 838 GATC DraI 274 TTTAAA TTT^AAA 699 TTTAAA HaeIII 87 GGCC GG^CC 173 GGCC MboI 195 GATC ^GATC 838 GATC MwoI 36 GCAGCCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1157 GCAATGGAGGC NdeII 195 GATC ^GATC 838 GATC NlaIII 348 CATG CATG^ 376 CATG NlaIV 411 GGAACC GG[ACGT]^[ACGT]CC 984 GGTACC Sau3AI 195 GATC ^GATC 838 GATC SfaNI 760 GATGC GCATC(5/9) 772 GATGC SmlI 595 CTTGAG C^T[CT][GA]AG 669 CTTAAG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AfaI AluI BbvI BfaI BsrI BssKI BstF5I BstNI DdeI EarI EcoRII Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeI MaeIII MboII MnlI MseI MslI MvaI RsaI ScrFI TfiI Tru9I TseI Tsp45I Tsp509I TspRI