Restriction map for
pFN21AE1879
(Linear) (Six-base) MAPSORT of: KIEE1879.seq Check: 9230 from: 1 to: 1212
KIEE1879.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AccI GT'mk_AC
Cuts at: 0 464 1212
Size: 464 748
Acc65I G'GTAC_C
Cuts at: 0 984 1212
Size: 984 228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1110 1212
Size: 1048 62 102
Fragments arranged by size:
1048 102 62
AflII C'TTAA_G
Cuts at: 0 669 1212
Size: 669 543
AflIII A'CryG_T
Cuts at: 0 347 1212
Size: 347 865
AleI CACnn'nnGTG
Cuts at: 0 1152 1212
Size: 1152 60
AseI AT'TA_AT
Cuts at: 0 290 1212
Size: 290 922
BanI G'GyrC_C
Cuts at: 0 984 1212
Size: 984 228
BbsI GAAGACnn'nnnn_
Cuts at: 0 1036 1061 1212
Size: 1036 25 151
Fragments arranged by size:
1036 151 25
BglII A'GATC_T
Cuts at: 0 837 1212
Size: 837 375
BlpI GC'TnA_GC
Cuts at: 0 29 827 1212
Size: 29 798 385
Fragments arranged by size:
798 385 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 1120 1212
Size: 237 883 92
Fragments arranged by size:
883 237 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1212
Size: 616 596
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 1212
Size: 145 1067
BseYI C'CCAG_C
Cuts at: 0 41 46 317 1212
Size: 41 5 271 895
Fragments arranged by size:
895 271 41 5
BsiHKAI G_wGCw'C
Cuts at: 0 1189 1212
Size: 1189 23
BsmI GAATG_Cn'
Cuts at: 0 399 1212
Size: 399 813
Bsp1286I G_dGCh'C
Cuts at: 0 1189 1212
Size: 1189 23
BspHI T'CATG_A
Cuts at: 0 375 1212
Size: 375 837
BsrDI GCAATG_nn'
Cuts at: 0 581 1164 1212
Size: 581 583 48
Fragments arranged by size:
583 581 48
BstEII G'GTnAC_C
Cuts at: 0 135 1212
Size: 135 1077
BstXI CCAn_nnnn'nTGG
Cuts at: 0 924 1212
Size: 924 288
BstYI r'GATC_y
Cuts at: 0 837 1212
Size: 837 375
BstZ17I GTA'TAC
Cuts at: 0 465 1212
Size: 465 747
Bsu36I CC'TnA_GG
Cuts at: 0 939 1212
Size: 939 273
BtsI GCAGTG_nn'
Cuts at: 0 660 1212
Size: 660 552
DraI TTT'AAA
Cuts at: 0 276 701 1212
Size: 276 425 511
Fragments arranged by size:
511 425 276
EaeI y'GGCC_r
Cuts at: 0 172 1212
Size: 172 1040
EarI CTCTTCn'nnn_
Cuts at: 0 88 120 653 1177 1212
Size: 88 32 533 524 35
Fragments arranged by size:
533 524 88 35 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 1048 1110 1120 1212
Size: 237 811 62 10 92
Fragments arranged by size:
811 237 92 62 10
EcoRV GAT'ATC
Cuts at: 0 666 1212
Size: 666 546
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 119 151 1052 1084 1212
Size: 119 32 901 32 128
Fragments arranged by size:
901 128 119 32 32
HincII GTy'rAC
Cuts at: 0 524 1212
Size: 524 688
KpnI G_GTAC'C
Cuts at: 0 988 1212
Size: 988 224
MscI TGG'CCA
Cuts at: 0 174 1212
Size: 174 1038
MslI CAynn'nnrTG
Cuts at: 0 152 922 1152 1212
Size: 152 770 230 60
Fragments arranged by size:
770 230 152 60
NspI r_CATG'y
Cuts at: 0 351 1212
Size: 351 861
PciI A'CATG_T
Cuts at: 0 347 1212
Size: 347 865
PshAI GACnn'nnGTC
Cuts at: 0 1143 1212
Size: 1143 69
SapI GCTCTTCn'nnn_
Cuts at: 0 1177 1212
Size: 1177 35
ScaI AGT'ACT
Cuts at: 0 1011 1212
Size: 1011 201
SmlI C'TyrA_G
Cuts at: 0 595 669 1212
Size: 595 74 543
Fragments arranged by size:
595 543 74
SpeI A'CTAG_T
Cuts at: 0 1012 1212
Size: 1012 200
TatI w'GTAC_w
Cuts at: 0 875 1009 1212
Size: 875 134 203
Fragments arranged by size:
875 203 134
XbaI T'CTAG_A
Cuts at: 0 435 1212
Size: 435 777
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AleI AseI BanI
BbsI BglII BlpI BpmI BpuEI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BstEII BstXI BstYI BstZ17I
Bsu36I BtsI DraI EaeI EarI Eco57MI EcoRV Hin4I
HincII KpnI MscI MslI NspI PciI PshAI SapI
ScaI SmlI SpeI TatI XbaI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AscI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BtgI BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII HindIII HpaI KasI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XcmI XhoI XmaI XmnI ZraI