################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## Acc65I AclI AcsI AflII AgeI AhdI AlwNI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BalI BamHI BbvCI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstXI BstYI BstZ17I ClaI CpoI DraI DrdI Eam1105I EarI EcoNI EcoRI EcoRV EcoT22I FbaI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MflI MluI MluNI MscI MseI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SexAI SfuI SmlI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 868 GACGTC GACGT^C AccI 871 GTCTAC GT^[AC][GT]AC AfaI 1156 GTAC GT^AC AflIII 1184 ACGTGT A^C[GA][CT]GT AocI 100 CCTCAGG CC^T[ACGT]AGG Aor51HI 941 AGCGCT AGC^GCT ApaI 562 GGGCCC GGGCC^C Asp700I 84 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1301 TGCGCA TGC^GCA BbsI 546 GAAGAC GAAGAC(2/6) BcgI 683 CGACCATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 546 GAAGAC GAAGAC(2/6) BseRI 1165 GAGGAG GAGGAG(10/8) BsgI 1368 GTGCAG GTGCAG(16/14) BsmAI 1451 GAGAC GTCTC(1/5) BsmBI 1451 GAGACG CGTCTC(1/5) BspHI 80 TCATGA T^CATGA BsrBI 723 GAGCGG CCGCTC(-3/-3) BssSI 1162 CACGAG CACGAG(-5/-1) BstEII 795 GGTGACC G^GT[ACGT]ACC BstPI 795 GGTGACC G^GT[ACGT]ACC Bsu36I 100 CCTCAGG CC^T[ACGT]AGG DraIII 1309 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 941 AGCGCT AGC^GCT Eco81I 100 CCTCAGG CC^T[ACGT]AGG EcoO65I 795 GGTGACC G^GT[ACGT]ACC FspI 1301 TGCGCA TGC^GCA NcoI 686 CCATGG C^CATGG NotI 298 GCGGCCGC GC^GGCCGC NspI 1409 ACATGC [GA]CATG^[CT] PstI 124 CTGCAG CTGCA^G RcaI 80 TCATGA T^CATGA RsaI 1156 GTAC GT^AC SbfI 123 CCTGCAGG CCTGCA^GG SfcI 124 CTGCAG C^T[GA][CT]AG SfiI 222 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 123 CCTGCAGG CCTGCA^GG StuI 857 AGGCCT AGG^CCT TaqI 505 TCGA T^CGA TthHB8I 505 TCGA T^CGA XmnI 84 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccIII 614 TCCGGA T^CCGGA 824 TCCGGA AlwI 1047 GGATC GGATC(4/5) 1134 GGATC BbrPI 973 CACGTG CAC^GTG 1183 CACGTG BsaAI 973 CACGTG [CT]AC^GT[GA] 1183 CACGTG BsaWI 614 TCCGGA [AT]^CCGG[AT] 824 TCCGGA BseAI 614 TCCGGA T^CCGGA 824 TCCGGA BspEI 614 TCCGGA T^CCGGA 824 TCCGGA BstAPI 1352 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1361 GCAAGCTGTGC DpnII 1048 GATC ^GATC 1135 GATC FseI 648 GGCCGGCC GGCCGG^CC 1401 GGCCGGCC HgaI 1453 GACGC GACGC(5/10) 529 GCGTC MboI 1048 GATC ^GATC 1135 GATC MroI 614 TCCGGA T^CCGGA 824 TCCGGA NdeII 1048 GATC ^GATC 1135 GATC PmaCI 973 CACGTG CAC^GTG 1183 CACGTG PmlI 973 CACGTG CAC^GTG 1183 CACGTG PpuMI 656 GGGTCCC [GA]G^G[AT]CC[CT] 1245 GGGTCCT PvuII 718 CAGCTG CAG^CTG 1396 CAGCTG Sau3AI 1048 GATC ^GATC 1135 GATC SgrAI 197 CGCCGGCG C[GA]^CCGG[CT]G 783 CGCCGGTG SmaI 62 CCCGGG CCC^GGG 1446 CCCGGG XmaCI 62 CCCGGG C^CCGGG 1446 CCCGGG XmaI 62 CCCGGG C^CCGGG 1446 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AcyI AluI Alw44I ApaLI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MaeIII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIII NlaIV PleI SacI SacII Sau96I ScrFI SfaNI SfoI StyI TseI Tsp45I TspRI