(Circular) (Six-base) MAPSORT of: pFN21AB5889.seq Check: 9067 from: 1 to: 6184 pFN21AB5889 6184 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3488 3488 Size: 6184 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2877 3994 278 Size: 53 83 186 936 1341 1117 2468 Fragments arranged by size: 2468 1341 1117 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2877 3527 1937 Size: 940 650 4594 Fragments arranged by size: 4594 940 650 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2405 4247 5631 5899 1632 Size: 773 1842 1384 268 1917 Fragments arranged by size: 1917 1842 1384 773 268 AfeI AGC'GCT Cuts at: 2948 2948 Size: 6184 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 3189 5083 6060 1203 Size: 1986 1894 977 1327 Fragments arranged by size: 1986 1894 1327 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 3192 1166 Size: 2026 4158 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6184 AlwNI CAG_nnn'CTG Cuts at: 3927 5499 3927 Size: 1572 4612 ApaI G_GGCC'C Cuts at: 2571 2571 Size: 6184 ApaLI G'TGCA_C Cuts at: 3002 3080 3239 3380 4241 5397 3002 Size: 78 159 141 861 1156 3789 Fragments arranged by size: 3789 1156 861 159 141 78 ApoI r'AATT_y Cuts at: 1493 3493 3719 3871 5980 1493 Size: 2000 226 152 2109 1697 Fragments arranged by size: 2109 2000 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 2067 2668 2815 3451 3509 1870 Size: 73 124 601 147 636 58 4545 Fragments arranged by size: 4545 636 601 147 124 73 58 BamHI G'GATC_C Cuts at: 3514 3881 3514 Size: 367 5817 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2206 2398 2428 2695 2759 Size: 325 217 627 419 192 30 267 64 Cuts at: 2759 3505 4960 618 Size: 746 1455 1842 Fragments arranged by size: 1842 1455 746 627 419 325 267 217 192 64 30 BanII G_rGCy'C Cuts at: 1391 2148 2244 2571 3212 3273 3336 3503 1391 Size: 757 96 327 641 61 63 167 4072 Fragments arranged by size: 4072 757 641 327 167 96 63 61 BbeI G_GCGC'C Cuts at: 2210 2402 2432 2210 Size: 192 30 5962 Fragments arranged by size: 5962 192 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2558 928 Size: 971 659 4554 Fragments arranged by size: 4554 971 659 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2677 2711 4375 4409 1903 Size: 34 740 34 1664 34 3678 Fragments arranged by size: 3678 1664 740 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4076 5286 4076 Size: 1210 4974 BclI T'GATC_A Cuts at: 1548 1548 Size: 6184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2204 2234 2480 Size: 107 122 71 631 605 532 30 246 Cuts at: 2480 2783 3347 3404 4795 136 Size: 303 564 57 1391 1525 Fragments arranged by size: 1525 1391 631 605 564 532 303 246 122 107 71 57 30 BglII A'GATC_T Cuts at: 6179 6179 Size: 6184 BlpI GC'TnA_GC Cuts at: 2142 2726 3206 3601 2142 Size: 584 480 395 4725 Fragments arranged by size: 4725 584 480 395 Bme1580I G_kGCm'C Cuts at: 1790 2571 2700 2721 2933 3006 3084 3243 3384 Size: 781 129 21 212 73 78 159 141 Cuts at: 3384 4245 5401 1790 Size: 861 1156 2573 Fragments arranged by size: 2573 1156 861 781 212 159 141 129 78 73 21 BmgBI CAC'GTC Cuts at: 1318 3070 1318 Size: 1752 4432 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3915 4873 442 Size: 416 53 83 399 24 2498 958 1753 Fragments arranged by size: 2498 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2231 3320 4844 1239 Size: 893 99 1089 1524 2579 Fragments arranged by size: 2579 1524 1089 893 99 Bpu10I CC'TnA_GC Cuts at: 2702 2711 2702 Size: 9 6175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4309 5174 5472 4309 Size: 865 298 5021 Fragments arranged by size: 5021 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4847 882 Size: 617 3348 2219 Fragments arranged by size: 3348 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2980 3190 3975 493 Size: 711 1776 210 785 2702 Fragments arranged by size: 2702 1776 785 711 210 BsaBI GATnn'nnATC Cuts at: 6178 6178 Size: 6184 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2207 2399 2429 2874 Size: 53 83 186 936 674 192 30 445 Cuts at: 2874 3459 3991 4373 5008 275 Size: 585 532 382 635 1451 Fragments arranged by size: 1451 936 674 635 585 532 445 382 192 186 83 53 30 BsaWI w'CCGG_w Cuts at: 2619 2829 4617 5015 5289 5436 5847 2619 Size: 210 1788 398 274 147 411 2956 Fragments arranged by size: 2956 1788 411 398 274 210 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3005 3035 1178 Size: 30 165 30 1602 30 4327 Fragments arranged by size: 4327 1602 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3185 1186 Size: 1999 4185 BseYI C'CCAG_C Cuts at: 2053 2721 3399 3465 5387 5883 2053 Size: 668 678 66 1922 496 2354 Fragments arranged by size: 2354 1922 678 668 496 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3394 1163 Size: 573 1658 3953 Fragments arranged by size: 3953 1658 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2238 2307 2439 2517 2532 2659 Size: 275 186 237 69 132 78 15 127 Cuts at: 2659 2811 3050 3634 4395 5423 1540 Size: 152 239 584 761 1028 2301 Fragments arranged by size: 2301 1028 761 584 275 239 237 186 152 132 127 78 69 15 BsiHKAI G_wGCw'C Cuts at: 2148 2271 3006 3084 3212 3243 3273 3384 4245 Size: 123 735 78 128 31 30 111 861 Cuts at: 4245 4330 5401 2148 Size: 85 1071 2931 Fragments arranged by size: 2931 1071 861 735 128 123 111 85 78 31 30 BsmI GAATG_Cn' Cuts at: 3695 3788 3695 Size: 93 6091 BsmBI CGTCTCn'nnnn_ Cuts at: 3450 3450 Size: 6184 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2244 2271 2571 2700 2721 2933 Size: 399 358 96 27 300 129 21 212 Cuts at: 2933 3006 3084 3212 3243 3273 3336 3384 3503 Size: 73 78 128 31 30 63 48 119 Cuts at: 3503 4245 4330 5401 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 358 300 212 129 128 119 96 85 78 73 63 48 31 30 27 21 BspEI T'CCGG_A Cuts at: 2619 2829 5847 2619 Size: 210 3018 2956 Fragments arranged by size: 3018 2956 210 BspHI T'CATG_A Cuts at: 2085 4073 5803 2085 Size: 1988 1730 2466 Fragments arranged by size: 2466 1988 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2122 3312 3488 3539 844 Size: 1278 1190 176 51 3489 Fragments arranged by size: 3489 1278 1190 176 51 BsrBI CCG'CTC Cuts at: 1394 1823 2730 4071 1394 Size: 429 907 1341 3507 Fragments arranged by size: 3507 1341 907 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4679 4853 65 Size: 1183 3431 174 1396 Fragments arranged by size: 3431 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2203 2654 2735 2762 2789 3407 4828 Size: 110 393 451 81 27 27 618 1421 Cuts at: 4828 5015 1700 Size: 187 2869 Fragments arranged by size: 2869 1421 618 451 393 187 110 81 27 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 3167 4244 5256 3167 Size: 1077 1012 4095 Fragments arranged by size: 4095 1077 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3363 3372 794 Size: 2569 9 3606 Fragments arranged by size: 3606 2569 9 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 2800 1184 Size: 1616 4568 BstYI r'GATC_y Cuts at: 1972 3514 3881 4266 4283 5735 5821 5833 6179 Size: 1542 367 385 17 1452 86 12 346 Cuts at: 6179 1972 Size: 1977 Fragments arranged by size: 1977 1542 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2106 2106 Size: 6184 BtgI C'CryG_G Cuts at: 513 1065 2292 2433 2475 2520 2691 3291 3328 Size: 552 1227 141 42 45 171 600 37 Cuts at: 3328 3344 513 Size: 16 3353 Fragments arranged by size: 3353 1227 600 552 171 141 45 42 37 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5445 Fragments arranged by size: 5445 683 56 BtsI GCAGTG_nn' Cuts at: 2787 3382 3700 4494 4514 2787 Size: 595 318 794 20 4457 Fragments arranged by size: 4457 794 595 318 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6149 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DraIII CAC_nnn'GTG Cuts at: 3319 3319 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5191 809 Size: 507 3875 1802 Fragments arranged by size: 3875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2235 2304 2436 Size: 54 1425 50 275 18 405 69 132 Cuts at: 2436 2484 2656 2784 3047 3405 3409 3631 4520 Size: 48 172 128 263 358 4 222 889 Cuts at: 4520 6149 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 405 358 275 263 222 172 132 128 69 54 50 48 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2235 2304 2436 2656 3047 3631 1537 Size: 275 423 69 132 220 391 584 4090 Fragments arranged by size: 4090 584 423 391 275 220 132 69 EarI CTCTTCn'nnn_ Cuts at: 1353 4114 1353 Size: 2761 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2278 2397 2449 4771 5145 5291 Size: 1064 138 689 119 52 2322 374 146 Cuts at: 5291 387 Size: 1280 Fragments arranged by size: 2322 1280 1064 689 374 146 138 119 52 EcoICRI GAG'CTC Cuts at: 2146 3210 3271 2146 Size: 1064 61 5059 Fragments arranged by size: 5059 1064 61 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2231 2405 3320 4247 4844 5631 Size: 393 500 99 174 915 927 597 787 Cuts at: 5631 5899 1239 Size: 268 1524 Fragments arranged by size: 1524 927 915 787 597 500 393 268 174 99 EcoO109I rG'GnC_Cy Cuts at: 2227 2567 2568 2662 3251 3340 2227 Size: 340 1 94 589 89 5071 Fragments arranged by size: 5071 589 340 94 89 1 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FseI GG_CCGG'CC Cuts at: 2658 3411 2658 Size: 753 5431 FspI TGC'GCA Cuts at: 3308 4690 3308 Size: 1382 4802 HaeII r_GCGC'y Cuts at: 1678 2210 2402 2432 2775 2950 3000 5331 1678 Size: 532 192 30 343 175 50 2331 2531 Fragments arranged by size: 2531 2331 532 343 192 175 50 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 4825 4857 4899 Size: 32 135 32 563 32 2658 32 42 Cuts at: 4899 4931 1373 Size: 32 2626 Fragments arranged by size: 2658 2626 563 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3528 3774 669 Size: 988 281 1590 246 3079 Fragments arranged by size: 3079 1590 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KasI G'GCGC_C Cuts at: 2206 2398 2428 2206 Size: 192 30 5962 Fragments arranged by size: 5962 192 30 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5298 5482 1305 Size: 3993 184 2007 Fragments arranged by size: 3993 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4362 Fragments arranged by size: 4362 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3192 3236 3264 4142 4501 4660 518 Size: 648 2026 44 28 878 359 159 2042 Fragments arranged by size: 2042 2026 878 648 359 159 44 28 MspA1I CmG'CkG Cuts at: 1606 2294 2309 2318 2378 2435 2477 2725 3346 Size: 688 15 9 60 57 42 248 621 Cuts at: 3346 3403 3600 4277 5425 5670 1606 Size: 57 197 677 1148 245 2120 Fragments arranged by size: 2120 1148 688 677 621 248 245 197 60 57 57 42 15 9 NaeI GCC'GGC Cuts at: 2205 2656 2737 2764 3409 2205 Size: 451 81 27 645 4980 Fragments arranged by size: 4980 645 451 81 27 NarI GG'CG_CC Cuts at: 2207 2399 2429 2207 Size: 192 30 5962 Fragments arranged by size: 5962 192 30 NcoI C'CATG_G Cuts at: 513 1065 2691 513 Size: 552 1626 4006 Fragments arranged by size: 4006 1626 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NgoMIV G'CCGG_C Cuts at: 2203 2654 2735 2762 3407 2203 Size: 451 81 27 645 4980 Fragments arranged by size: 4980 645 451 81 27 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 2304 3631 2304 Size: 1327 4857 NspI r_CATG'y Cuts at: 1132 1229 3418 3542 5087 1132 Size: 97 2189 124 1545 2229 Fragments arranged by size: 2229 2189 1545 124 97 PasI CC'CwG_GG Cuts at: 1348 2170 1348 Size: 822 5362 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PmlI CAC'GTG Cuts at: 2980 3190 2980 Size: 210 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2662 3251 2662 Size: 589 5595 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6184 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PspOMI G'GGCC_C Cuts at: 2567 2567 Size: 6184 PstI C_TGCA'G Cuts at: 830 2133 3536 830 Size: 1303 1403 3478 Fragments arranged by size: 3478 1403 1303 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 PvuII CAG'CTG Cuts at: 2725 3403 2725 Size: 678 5506 SacI G_AGCT'C Cuts at: 2148 3212 3273 2148 Size: 1064 61 5059 Fragments arranged by size: 5059 1064 61 SacII CC_GC'GG Cuts at: 2295 2436 2478 3347 2295 Size: 141 42 869 5132 Fragments arranged by size: 5132 869 141 42 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SanDI GG'GwC_CC Cuts at: 2662 2662 Size: 6184 SbfI CC_TGCA'GG Cuts at: 2133 3536 2133 Size: 1403 4781 ScaI AGT'ACT Cuts at: 4432 6144 4432 Size: 1712 4472 SfcI C'TryA_G Cuts at: 826 1045 2129 3532 4667 5348 5539 826 Size: 219 1084 1403 1135 681 191 1471 Fragments arranged by size: 1471 1403 1135 1084 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2234 2234 Size: 6184 SfoI GGC'GCC Cuts at: 2208 2400 2430 2208 Size: 192 30 5962 Fragments arranged by size: 5962 192 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 2203 2789 1700 Size: 503 586 5095 Fragments arranged by size: 5095 586 503 SmaI CCC'GGG Cuts at: 1872 2069 3453 3511 1872 Size: 197 1384 58 4545 Fragments arranged by size: 4545 1384 197 58 SmlI C'TyrA_G Cuts at: 820 1017 1943 4288 5189 5451 820 Size: 197 926 2345 901 262 1553 Fragments arranged by size: 2345 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 4108 5 Size: 47 4056 2081 Fragments arranged by size: 4056 2081 47 StuI AGG'CCT Cuts at: 1517 2864 1517 Size: 1347 4837 StyI C'CwwG_G Cuts at: 513 1065 2541 2592 2649 2691 3623 513 Size: 552 1476 51 57 42 932 3074 Fragments arranged by size: 3074 1476 932 552 57 51 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4211 4228 4381 4566 5011 325 Size: 1255 2631 17 153 185 445 1498 Fragments arranged by size: 2631 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4430 6142 96 Size: 274 80 33 51 1443 2453 1712 138 Fragments arranged by size: 2453 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6144 6176 6144 Size: 32 6152 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6184 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 2067 3451 3509 1870 Size: 197 1384 58 4545 Fragments arranged by size: 4545 1384 197 58 XmnI GAAnn'nnTTC Cuts at: 2093 4313 2093 Size: 2220 3964 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2875 3992 276 Size: 53 83 186 936 1341 1117 2468 Fragments arranged by size: 2468 1341 1117 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SapI SexAI SrfI SwaI XcmI