################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfrI BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 1494 GACGTC GACGT^C AocI 1441 CCTCAGG CC^T[ACGT]AGG ApaI 84 GGGCCC GGGCC^C AspHI 855 GAGCTC G[AT]GC[AT]^C BalI 290 TGGCCA TGG^CCA BfaI 1240 CTAG C^TAG BglI 323 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 662 AGATCT A^GATCT BlpI 634 GCTGAGC GC^T[ACGT]AGC Bpu1102I 634 GCTGAGC GC^T[ACGT]AGC BsgI 472 CTGCAC GTGCAG(16/14) BsiEI 41 CGGCCG CG[GA][CT]^CG BsiHKAI 855 GAGCTC G[AT]GC[AT]^C BsrDI 603 CATTGC GCAATG(2/0) BstAPI 1378 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1441 CCTCAGG CC^T[ACGT]AGG CelII 634 GCTGAGC GC^T[ACGT]AGC EagI 41 CGGCCG C^GGCCG EarI 6 GAAGAG CTCTTC(1/4) EclXI 41 CGGCCG C^GGCCG Eco52I 41 CGGCCG C^GGCCG Eco81I 1441 CCTCAGG CC^T[ACGT]AGG EcoNI 75 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 91 GACGC GACGC(5/10) Ksp632I 6 GAAGAG CTCTTC(1/4) KspI 38 CCGCGG CCGC^GG MaeI 1240 CTAG C^TAG MluNI 290 TGGCCA TGG^CCA MscI 290 TGGCCA TGG^CCA NcoI 1361 CCATGG C^CATGG NdeI 625 CATATG CA^TATG PflMI 1044 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 855 GAGCTC GAGCT^C SacII 38 CCGCGG CCGC^GG SapI 6 GAAGAGC GCTCTTC(1/4) SexAI 1191 ACCTGGT A^CC[AT]GGT SgrAI 758 CGCCGGTG C[GA]^CCGG[CT]G TfiI 309 GATTC G^A[AT]TC TspRI 1138 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 1044 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1169 CCAAGGCTATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 425 ACATGT A^C[GA][CT]GT 878 ACATGT AlwI 711 GATCC GGATC(4/5) 729 GATCC AlwNI 364 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 1187 CAGAACCTG BbeI 196 GGCGCC GGCGC^C 597 GGCGCC BseRI 802 CTCCTC GAGGAG(10/8) 574 GAGGAG BspLU11I 425 ACATGT A^CATGT 878 ACATGT BspMI 470 ACCTGC ACCTGC(4/8) 677 ACCTGC BstYI 662 AGATCT [GA]^GATC[CT] 728 AGATCC EcoT22I 622 ATGCAT ATGCA^T 1385 ATGCAT KasI 196 GGCGCC G^GCGCC 597 GGCGCC MaeII 455 ACGT A^CGT 1495 ACGT MflI 662 AGATCT [GA]^GATC[CT] 728 AGATCC MslI 621 CATGCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1362 CATGGTGGTG NarI 196 GGCGCC GG^CGCC 597 GGCGCC NsiI 622 ATGCAT ATGCA^T 1385 ATGCAT PpuMI 437 GGGACCC [GA]G^G[AT]CC[CT] 530 AGGACCT PstI 1135 CTGCAG CTGCA^G 1376 CTGCAG SfoI 196 GGCGCC GGC^GCC 597 GGCGCC StuI 551 AGGCCT AGG^CCT 1031 AGGCCT Tsp45I 467 GTGAC ^GT[GC]AC 1066 GTGAC XhoII 662 AGATCT [GA]^GATC[CT] 728 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI AvaII BanI BanII BbvCI BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TseI TthHB8I XmaCI XmaI