(Circular) (Six-base) MAPSORT of: pFN21AE1951.seq Check: 6258 from: 1 to: 6229 pFN21AE1951 6229 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3503 4039 278 Size: 53 83 186 936 1967 536 2468 Fragments arranged by size: 2468 1967 936 536 186 83 53 AccI GT'mk_AC Cuts at: 1937 3572 1937 Size: 1635 4594 Acc65I G'GTAC_C Cuts at: 3550 3550 Size: 6229 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 2957 4292 5676 5944 1632 Size: 941 384 1335 1384 268 1917 Fragments arranged by size: 1917 1384 1335 941 384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 2430 2883 5128 6105 1203 Size: 1227 453 2245 977 1327 Fragments arranged by size: 2245 1327 1227 977 453 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 4958 4958 Size: 6229 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2652 1184 Size: 1468 4761 AlwNI CAG_nnn'CTG Cuts at: 2374 3197 3972 5544 2374 Size: 823 775 1572 3059 Fragments arranged by size: 3059 1572 823 775 ApaI G_GGCC'C Cuts at: 2093 2093 Size: 6229 ApaLI G'TGCA_C Cuts at: 4286 5442 4286 Size: 1156 5073 ApoI r'AATT_y Cuts at: 1493 3538 3764 3916 6025 1493 Size: 2045 226 152 2109 1697 Fragments arranged by size: 2109 2045 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4783 160 Size: 872 3751 1606 Fragments arranged by size: 3751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2126 2514 2576 3129 3554 1870 Size: 73 127 56 388 62 553 425 4545 Fragments arranged by size: 4545 553 425 388 127 73 62 56 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2201 2325 2602 2655 3170 Size: 325 217 627 414 124 277 53 515 Cuts at: 3170 3550 5005 618 Size: 380 1455 1842 Fragments arranged by size: 1842 1455 627 515 414 380 325 277 217 124 53 BanII G_rGCy'C Cuts at: 1391 2093 2195 2864 2954 3548 1391 Size: 702 102 669 90 594 4072 Fragments arranged by size: 4072 702 669 594 102 90 BbeI G_GCGC'C Cuts at: 2205 2606 2205 Size: 401 5828 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5258 BbvCI CC'TCA_GC Cuts at: 2240 2783 3014 2240 Size: 543 231 5455 Fragments arranged by size: 5455 543 231 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4420 4454 1903 Size: 34 2483 34 3678 Fragments arranged by size: 3678 2483 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4121 5331 4121 Size: 1210 5019 BclI T'GATC_A Cuts at: 1548 1548 Size: 6229 BfrBI ATG'CAT Cuts at: 2629 3392 2629 Size: 763 5466 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2334 4840 136 Size: 107 122 71 631 605 662 2506 1525 Fragments arranged by size: 2506 1525 662 631 605 122 107 71 BglII A'GATC_T Cuts at: 2667 6224 2667 Size: 3557 2672 BlpI GC'TnA_GC Cuts at: 2640 3646 2640 Size: 1006 5223 Bme1580I G_kGCm'C Cuts at: 1790 2093 3414 4290 5446 1790 Size: 303 1321 876 1156 2573 Fragments arranged by size: 2573 1321 1156 876 303 BmgBI CAC'GTC Cuts at: 1318 2461 1318 Size: 1143 5086 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2290 3960 4918 Size: 416 53 83 399 24 873 1670 958 Cuts at: 4918 442 Size: 1753 Fragments arranged by size: 1753 1670 958 873 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 2252 2729 2878 3359 4889 1239 Size: 998 15 477 149 481 1530 2579 Fragments arranged by size: 2579 1530 998 481 477 149 15 Bpu10I CC'TnA_GC Cuts at: 2240 2783 3014 2240 Size: 543 231 5455 Fragments arranged by size: 5455 543 231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4354 5219 5517 4354 Size: 865 298 5066 Fragments arranged by size: 5066 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2984 3119 3472 4892 882 Size: 617 1485 135 353 1420 2219 Fragments arranged by size: 2219 1485 1420 617 353 135 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 6223 6223 Size: 6229 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2097 2202 2603 3500 Size: 53 83 186 936 564 105 401 897 Cuts at: 3500 4036 4418 5053 275 Size: 536 382 635 1451 Fragments arranged by size: 1451 936 897 635 564 536 401 382 186 105 83 53 BsaWI w'CCGG_w Cuts at: 4662 5060 5334 5481 5892 4662 Size: 398 274 147 411 4999 Fragments arranged by size: 4999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2243 2273 2373 2403 3467 Size: 30 165 30 840 30 100 30 1064 Cuts at: 3467 3497 1178 Size: 30 3910 Fragments arranged by size: 3910 1064 840 165 100 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2594 2798 1186 Size: 1408 204 4617 Fragments arranged by size: 4617 1408 204 BseYI C'CCAG_C Cuts at: 2947 3349 5432 5928 2947 Size: 402 2083 496 3248 Fragments arranged by size: 3248 2083 496 402 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2462 1163 Size: 573 726 4930 Fragments arranged by size: 4930 726 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 3679 4440 5468 1540 Size: 275 186 48 1630 761 1028 2301 Fragments arranged by size: 2301 1630 1028 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 2864 4290 4375 5446 2864 Size: 1426 85 1071 3647 Fragments arranged by size: 3647 1426 1071 85 BsmI GAATG_Cn' Cuts at: 3740 3833 3740 Size: 93 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2195 2864 2954 3414 3548 4290 Size: 399 303 102 669 90 460 134 742 Cuts at: 4290 4375 5446 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 669 460 399 303 134 102 90 85 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2484 2691 3584 844 Size: 1640 207 893 3489 Fragments arranged by size: 3489 1640 893 207 BsrBI CCG'CTC Cuts at: 1394 1823 2806 3150 3483 4116 1394 Size: 429 983 344 333 633 3507 Fragments arranged by size: 3507 983 633 429 344 333 BsrDI GCAATG_nn' Cuts at: 65 1248 2607 4724 4898 65 Size: 1183 1359 2117 174 1396 Fragments arranged by size: 2117 1396 1359 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2481 2571 2764 4873 5060 1700 Size: 110 671 90 193 2109 187 2869 Fragments arranged by size: 2869 2109 671 193 187 110 90 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6229 BssHII G'CGCG_C Cuts at: 1925 2035 2037 2211 1925 Size: 110 2 174 5943 Fragments arranged by size: 5943 174 110 2 BssSI C'ACGA_G Cuts at: 4289 5301 4289 Size: 1012 5217 BstAPI GCAn_nnn'nTGC Cuts at: 794 3389 794 Size: 2595 3634 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6229 BstXI CCAn_nnnn'nTGG Cuts at: 2263 2455 3373 2263 Size: 192 918 5119 Fragments arranged by size: 5119 918 192 BstYI r'GATC_y Cuts at: 1972 2667 2733 3559 3926 4311 4328 5780 5866 Size: 695 66 826 367 385 17 1452 86 Cuts at: 5866 5878 6224 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 826 695 385 367 346 86 66 17 12 Bsu36I CC'TnA_GG Cuts at: 3447 3447 Size: 6229 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2524 3366 513 Size: 552 939 39 481 842 3376 Fragments arranged by size: 3376 939 842 552 481 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5490 Fragments arranged by size: 5490 683 56 BtsI GCAGTG_nn' Cuts at: 3149 3745 4539 4559 3149 Size: 596 794 20 4819 Fragments arranged by size: 4819 794 596 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 3533 3533 Size: 6229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2295 2527 Size: 54 1425 50 275 18 216 249 232 Cuts at: 2527 3375 3676 4565 6194 8 Size: 848 301 889 1629 43 Fragments arranged by size: 1629 1425 889 848 301 275 249 232 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 3676 1537 Size: 275 234 1630 4090 Fragments arranged by size: 4090 1630 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2006 4159 1353 Size: 653 2153 3423 Fragments arranged by size: 3423 2153 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3224 4816 5190 5336 387 Size: 1064 138 1635 1592 374 146 1280 Fragments arranged by size: 1635 1592 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2862 2862 Size: 6229 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2252 2573 2729 2878 2957 3359 Size: 393 605 15 321 156 149 79 402 Cuts at: 3359 4292 4889 5676 5944 1239 Size: 933 597 787 268 1524 Fragments arranged by size: 1524 933 787 605 597 402 393 321 268 156 149 79 15 EcoNI CCTnn'n_nnAGG Cuts at: 2084 2084 Size: 6229 EcoO109I rG'GnC_Cy Cuts at: 2089 2090 2443 2536 2089 Size: 1 353 93 5782 Fragments arranged by size: 5782 353 93 1 EcoRI G'AATT_C Cuts at: 3538 3916 3538 Size: 378 5851 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2197 2929 3199 2197 Size: 732 270 5227 Fragments arranged by size: 5227 732 270 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 2164 2205 2606 3082 3118 5376 1678 Size: 486 41 401 476 36 2258 2531 Fragments arranged by size: 2531 2258 486 476 401 41 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4870 4902 4944 4976 1373 Size: 32 135 32 3298 32 42 32 2626 Fragments arranged by size: 3298 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3573 3819 669 Size: 988 281 1635 246 3079 Fragments arranged by size: 3079 1635 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KasI G'GCGC_C Cuts at: 2201 2602 2201 Size: 401 5828 KpnI G_GTAC'C Cuts at: 3554 3554 Size: 6229 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5343 5527 1305 Size: 4038 184 2007 Fragments arranged by size: 4038 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 10 Size: 54 1425 343 465 3942 Fragments arranged by size: 3942 1425 465 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2630 3371 4187 4546 4705 518 Size: 648 1464 741 816 359 159 2042 Fragments arranged by size: 2042 1464 816 741 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2045 2615 2722 3109 3310 3337 3380 3645 Size: 439 570 107 387 201 27 43 265 Cuts at: 3645 4322 5470 5715 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 570 439 387 265 245 201 107 43 27 NarI GG'CG_CC Cuts at: 2202 2603 2202 Size: 401 5828 NcoI C'CATG_G Cuts at: 513 1065 2004 3366 513 Size: 552 939 1362 3376 Fragments arranged by size: 3376 1362 939 552 NdeI CA'TA_TG Cuts at: 387 2631 387 Size: 2244 3985 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NsiI A_TGCA'T Cuts at: 2631 3394 2631 Size: 763 5466 NspI r_CATG'y Cuts at: 1132 1229 2272 2434 2887 3396 3410 3587 5132 Size: 97 1043 162 453 509 14 177 1545 Cuts at: 5132 1132 Size: 2229 Fragments arranged by size: 2229 1545 1043 509 453 177 162 97 14 PasI CC'CwG_GG Cuts at: 1348 2086 2438 1348 Size: 738 352 5139 Fragments arranged by size: 5139 738 352 PciI A'CATG_T Cuts at: 2430 2883 5128 2430 Size: 453 2245 3531 Fragments arranged by size: 3531 2245 453 PflMI CCAn_nnn'nTGG Cuts at: 1108 3055 1108 Size: 1947 4282 PfoI T'CCnGG_A Cuts at: 3048 3048 Size: 6229 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5811 5843 4298 Size: 32 1481 32 4684 Fragments arranged by size: 4684 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2443 2536 2443 Size: 93 6136 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PspOMI G'GGCC_C Cuts at: 2089 2089 Size: 6229 PstI C_TGCA'G Cuts at: 830 3144 3385 3581 830 Size: 2314 241 196 3478 Fragments arranged by size: 3478 2314 241 196 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 2722 3109 3337 2722 Size: 387 228 5614 Fragments arranged by size: 5614 387 228 SacI G_AGCT'C Cuts at: 2864 2864 Size: 6229 SacII CC_GC'GG Cuts at: 2046 2046 Size: 6229 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SanDI GG'GwC_CC Cuts at: 2443 2443 Size: 6229 SapI GCTCTTCn'nnn_ Cuts at: 2006 2006 Size: 6229 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 4477 6189 4477 Size: 1712 4517 SexAI A'CCwGG_T Cuts at: 3196 3196 Size: 6229 SfcI C'TryA_G Cuts at: 826 1045 2695 3140 3381 3577 4712 5393 5584 Size: 219 1650 445 241 196 1135 681 191 Cuts at: 5584 826 Size: 1471 Fragments arranged by size: 1650 1471 1135 681 445 241 219 196 191 SfoI GGC'GCC Cuts at: 2203 2604 2203 Size: 401 5828 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 2764 1700 Size: 1064 5165 SmaI CCC'GGG Cuts at: 1872 2072 2516 3131 3556 1872 Size: 200 444 615 425 4545 Fragments arranged by size: 4545 615 444 425 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 4333 5234 5496 820 Size: 197 926 2390 901 262 1553 Fragments arranged by size: 2390 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3587 1132 Size: 2455 3774 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 2558 3038 1517 Size: 1041 480 4708 Fragments arranged by size: 4708 1041 480 StyI C'CwwG_G Cuts at: 513 1065 2004 3174 3366 3432 3668 513 Size: 552 939 1170 192 66 236 3074 Fragments arranged by size: 3074 1170 939 552 236 192 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3301 4256 4273 4426 4611 5056 325 Size: 1255 1721 955 17 153 185 445 1498 Fragments arranged by size: 1721 1498 1255 955 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4475 6187 96 Size: 274 80 33 51 1443 2498 1712 138 Fragments arranged by size: 2498 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2243 2275 2373 2405 2435 2467 3216 3248 3467 Size: 32 98 32 30 32 749 32 219 Cuts at: 3467 3499 6189 6221 2243 Size: 32 2690 32 2251 Fragments arranged by size: 2690 2251 749 219 98 32 32 32 32 32 32 30 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6229 XbaI T'CTAG_A Cuts at: 3565 3565 Size: 6229 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3181 3181 Size: 6229 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 2070 2514 3129 3554 1870 Size: 200 444 615 425 4545 Fragments arranged by size: 4545 615 444 425 200 XmnI GAAnn'nnTTC Cuts at: 4358 4358 Size: 6229 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3501 4037 276 Size: 53 83 186 936 1967 536 2468 Fragments arranged by size: 2468 1967 936 536 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SfiI SrfI SwaI