Restriction map for
pFN21AE1951
(Linear) (Six-base) MAPSORT of: KIEE1951.seq Check: 9086 from: 1 to: 1524
KIEE1951.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AatII G_ACGT'C
Cuts at: 0 1498 1524
Size: 1498 26
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 952 1524
Size: 568 384 572
Fragments arranged by size:
572 568 384
AflIII A'CryG_T
Cuts at: 0 425 878 1524
Size: 425 453 646
Fragments arranged by size:
646 453 425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 647 1524
Size: 647 877
AlwNI CAG_nnn'CTG
Cuts at: 0 369 1192 1524
Size: 369 823 332
Fragments arranged by size:
823 369 332
ApaI G_GGCC'C
Cuts at: 0 88 1524
Size: 88 1436
AvaI C'yCGr_G
Cuts at: 0 65 121 509 571 1124 1524
Size: 65 56 388 62 553 400
Fragments arranged by size:
553 400 388 65 62 56
BanI G'GyrC_C
Cuts at: 0 196 320 597 650 1165 1524
Size: 196 124 277 53 515 359
Fragments arranged by size:
515 359 277 196 124 53
BanII G_rGCy'C
Cuts at: 0 88 190 859 949 1524
Size: 88 102 669 90 575
Fragments arranged by size:
669 575 102 90 88
BbeI G_GCGC'C
Cuts at: 0 200 601 1524
Size: 200 401 923
Fragments arranged by size:
923 401 200
BbvCI CC'TCA_GC
Cuts at: 0 235 778 1009 1524
Size: 235 543 231 515
Fragments arranged by size:
543 515 235 231
BfrBI ATG'CAT
Cuts at: 0 624 1387 1524
Size: 624 763 137
Fragments arranged by size:
763 624 137
BglI GCCn_nnn'nGGC
Cuts at: 0 329 1524
Size: 329 1195
BglII A'GATC_T
Cuts at: 0 662 1524
Size: 662 862
BlpI GC'TnA_GC
Cuts at: 0 635 1524
Size: 635 889
Bme1580I G_kGCm'C
Cuts at: 0 88 1409 1524
Size: 88 1321 115
Fragments arranged by size:
1321 115 88
BmgBI CAC'GTC
Cuts at: 0 456 1524
Size: 456 1068
BmrI ACTGGGnnnn_n'
Cuts at: 0 285 1524
Size: 285 1239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 247 724 873 1354 1524
Size: 232 15 477 149 481 170
Fragments arranged by size:
481 477 232 170 149 15
Bpu10I CC'TnA_GC
Cuts at: 0 235 778 1009 1524
Size: 235 543 231 515
Fragments arranged by size:
543 515 235 231
BsaI GGTCTCn'nnnn_
Cuts at: 0 979 1114 1467 1524
Size: 979 135 353 57
Fragments arranged by size:
979 353 135 57
BsaHI Gr'CG_yC
Cuts at: 0 92 197 598 1495 1524
Size: 92 105 401 897 29
Fragments arranged by size:
897 401 105 92 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 238 268 368 398 1462 1492 1524
Size: 238 30 100 30 1064 30 32
Fragments arranged by size:
1064 238 100 32 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 589 793 1524
Size: 589 204 731
Fragments arranged by size:
731 589 204
BseYI C'CCAG_C
Cuts at: 0 942 1344 1524
Size: 942 402 180
Fragments arranged by size:
942 402 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1524
Size: 457 1067
BsiEI CG_ry'CG
Cuts at: 0 44 1524
Size: 44 1480
BsiHKAI G_wGCw'C
Cuts at: 0 859 1524
Size: 859 665
Bsp1286I G_dGCh'C
Cuts at: 0 88 190 859 949 1409 1524
Size: 88 102 669 90 460 115
Fragments arranged by size:
669 460 115 102 90 88
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 479 686 1524
Size: 479 207 838
Fragments arranged by size:
838 479 207
BsrBI CCG'CTC
Cuts at: 0 801 1145 1478 1524
Size: 801 344 333 46
Fragments arranged by size:
801 344 333 46
BsrDI GCAATG_nn'
Cuts at: 0 602 1524
Size: 602 922
BsrFI r'CCGG_y
Cuts at: 0 476 566 759 1524
Size: 476 90 193 765
Fragments arranged by size:
765 476 193 90
BssHII G'CGCG_C
Cuts at: 0 30 32 206 1524
Size: 30 2 174 1318
Fragments arranged by size:
1318 174 30 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1384 1524
Size: 1384 140
BstXI CCAn_nnnn'nTGG
Cuts at: 0 258 450 1368 1524
Size: 258 192 918 156
Fragments arranged by size:
918 258 192 156
BstYI r'GATC_y
Cuts at: 0 662 728 1524
Size: 662 66 796
Fragments arranged by size:
796 662 66
Bsu36I CC'TnA_GG
Cuts at: 0 1442 1524
Size: 1442 82
BtgI C'CryG_G
Cuts at: 0 38 519 1361 1524
Size: 38 481 842 163
Fragments arranged by size:
842 481 163 38
BtsI GCAGTG_nn'
Cuts at: 0 1144 1524
Size: 1144 380
EaeI y'GGCC_r
Cuts at: 0 41 290 522 1370 1524
Size: 41 249 232 848 154
Fragments arranged by size:
848 249 232 154 41
EagI C'GGCC_G
Cuts at: 0 41 1524
Size: 41 1483
EarI CTCTTCn'nnn_
Cuts at: 0 1 1524
Size: 1 1523
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1219 1524
Size: 1219 305
EcoICRI GAG'CTC
Cuts at: 0 857 1524
Size: 857 667
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 247 568 724 873 952 1354 1524
Size: 232 15 321 156 149 79 402 170
Fragments arranged by size:
402 321 232 170 156 149 79 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 1524
Size: 79 1445
EcoO109I rG'GnC_Cy
Cuts at: 0 84 85 438 531 1524
Size: 84 1 353 93 993
Fragments arranged by size:
993 353 93 84 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 192 924 1194 1524
Size: 192 732 270 330
Fragments arranged by size:
732 330 270 192
HaeII r_GCGC'y
Cuts at: 0 159 200 601 1077 1113 1524
Size: 159 41 401 476 36 411
Fragments arranged by size:
476 411 401 159 41 36
KasI G'GCGC_C
Cuts at: 0 196 597 1524
Size: 196 401 927
Fragments arranged by size:
927 401 196
MscI TGG'CCA
Cuts at: 0 292 1524
Size: 292 1232
MslI CAynn'nnrTG
Cuts at: 0 625 1366 1524
Size: 625 741 158
Fragments arranged by size:
741 625 158
MspA1I CmG'CkG
Cuts at: 0 40 610 717 1104 1305 1332 1375 1524
Size: 40 570 107 387 201 27 43 149
Fragments arranged by size:
570 387 201 149 107 43 40 27
NarI GG'CG_CC
Cuts at: 0 197 598 1524
Size: 197 401 926
Fragments arranged by size:
926 401 197
NcoI C'CATG_G
Cuts at: 0 1361 1524
Size: 1361 163
NdeI CA'TA_TG
Cuts at: 0 626 1524
Size: 626 898
NsiI A_TGCA'T
Cuts at: 0 626 1389 1524
Size: 626 763 135
Fragments arranged by size:
763 626 135
NspI r_CATG'y
Cuts at: 0 267 429 882 1391 1405 1524
Size: 267 162 453 509 14 119
Fragments arranged by size:
509 453 267 162 119 14
PasI CC'CwG_GG
Cuts at: 0 81 433 1524
Size: 81 352 1091
Fragments arranged by size:
1091 352 81
PciI A'CATG_T
Cuts at: 0 425 878 1524
Size: 425 453 646
Fragments arranged by size:
646 453 425
PflMI CCAn_nnn'nTGG
Cuts at: 0 1050 1524
Size: 1050 474
PfoI T'CCnGG_A
Cuts at: 0 1043 1524
Size: 1043 481
PpuMI rG'GwC_Cy
Cuts at: 0 438 531 1524
Size: 438 93 993
Fragments arranged by size:
993 438 93
PspOMI G'GGCC_C
Cuts at: 0 84 1524
Size: 84 1440
PstI C_TGCA'G
Cuts at: 0 1139 1380 1524
Size: 1139 241 144
Fragments arranged by size:
1139 241 144
PvuII CAG'CTG
Cuts at: 0 717 1104 1332 1524
Size: 717 387 228 192
Fragments arranged by size:
717 387 228 192
SacI G_AGCT'C
Cuts at: 0 859 1524
Size: 859 665
SacII CC_GC'GG
Cuts at: 0 41 1524
Size: 41 1483
SanDI GG'GwC_CC
Cuts at: 0 438 1524
Size: 438 1086
SapI GCTCTTCn'nnn_
Cuts at: 0 1 1524
Size: 1 1523
SexAI A'CCwGG_T
Cuts at: 0 1191 1524
Size: 1191 333
SfcI C'TryA_G
Cuts at: 0 690 1135 1376 1524
Size: 690 445 241 148
Fragments arranged by size:
690 445 241 148
SfoI GGC'GCC
Cuts at: 0 198 599 1524
Size: 198 401 925
Fragments arranged by size:
925 401 198
SgrAI Cr'CCGG_yG
Cuts at: 0 759 1524
Size: 759 765
SmaI CCC'GGG
Cuts at: 0 67 511 1126 1524
Size: 67 444 615 398
Fragments arranged by size:
615 444 398 67
StuI AGG'CCT
Cuts at: 0 553 1033 1524
Size: 553 480 491
Fragments arranged by size:
553 491 480
StyI C'CwwG_G
Cuts at: 0 1169 1361 1427 1524
Size: 1169 192 66 97
Fragments arranged by size:
1169 192 97 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1296 1524
Size: 1296 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 238 270 368 400 430 462 1211 1243
Size: 238 32 98 32 30 32 749 32
Cuts at: 1243 1462 1494 1524
Size: 219 32 30
Fragments arranged by size:
749 238 219 98 32 32 32 32
32 30 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1176 1524
Size: 1176 348
XmaI C'CCGG_G
Cuts at: 0 65 509 1124 1524
Size: 65 444 615 400
Fragments arranged by size:
615 444 400 65
ZraI GAC'GTC
Cuts at: 0 1496 1524
Size: 1496 28
Enzymes that do cut:
AatII AcuI AflIII AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbvCI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII KasI MscI MslI MspA1I NarI NcoI
NdeI NsiI NspI PasI PciI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SacII SanDI SapI SexAI
SfcI SfoI SgrAI SmaI StuI StyI TaqII TstI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BmtI BplI
BpuEI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI NaeI NgoMIV NheI NotI NruI PacI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SbfI ScaI SfiI SgfI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmnI