Restriction map for
pFN21AE0164
(Circular) (Six-base) MAPSORT of: pFN21AE0164.seq Check: 6927 from: 1 to: 5119
pFN21AE0164 5119 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2140 2140
Size: 5119
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2929 278
Size: 53 83 186 936 1393 2468
Fragments arranged by size:
2468 1393 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2462 1937
Size: 525 4594
Acc65I G'GTAC_C
Cuts at: 2440 2440
Size: 5119
AclI AA'CG_TT
Cuts at: 1563 3246 3619 1563
Size: 1683 373 3063
Fragments arranged by size:
3063 1683 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2291 3182 4566 4834 1632
Size: 659 891 1384 268 1917
Fragments arranged by size:
1917 1384 891 659 268
AfeI AGC'GCT
Cuts at: 2171 2171
Size: 5119
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4922
AflIII A'CryG_T
Cuts at: 1203 4018 4995 1203
Size: 2815 977 1327
Fragments arranged by size:
2815 1327 977
AgeI A'CCGG_T
Cuts at: 3950 3950
Size: 5119
AhdI GACnn_n'nnGTC
Cuts at: 3848 3848
Size: 5119
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5119
AlwNI CAG_nnn'CTG
Cuts at: 2323 2862 4434 2323
Size: 539 1572 3008
Fragments arranged by size:
3008 1572 539
ApaLI G'TGCA_C
Cuts at: 2153 2248 3176 4332 2153
Size: 95 928 1156 2940
Fragments arranged by size:
2940 1156 928 95
ApoI r'AATT_y
Cuts at: 1493 2428 2654 2806 4915 1493
Size: 935 226 152 2109 1697
Fragments arranged by size:
2109 1697 935 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3673 160
Size: 872 2641 1606
Fragments arranged by size:
2641 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5119
AvaI C'yCGr_G
Cuts at: 1870 1943 2444 1870
Size: 73 501 4545
Fragments arranged by size:
4545 501 73
BamHI G'GATC_C
Cuts at: 2449 2816 2449
Size: 367 4752
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2440 3895 618
Size: 325 217 627 653 1455 1842
Fragments arranged by size:
1842 1455 653 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2438 1391
Size: 1047 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3310 3344 1903
Size: 34 1373 34 3678
Fragments arranged by size:
3678 1373 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3011 4221 3011
Size: 1210 3909
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5119
BfrBI ATG'CAT
Cuts at: 2257 2257
Size: 5119
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3730 136
Size: 107 122 71 631 605 2058 1525
Fragments arranged by size:
2058 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5114 5114
Size: 5119
BlpI GC'TnA_GC
Cuts at: 2010 2536 2010
Size: 526 4593
Bme1580I G_kGCm'C
Cuts at: 1790 2157 2252 3180 4336 1790
Size: 367 95 928 1156 2573
Fragments arranged by size:
2573 1156 928 367 95
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5119
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2850 3808 442
Size: 416 53 83 399 24 1433 958 1753
Fragments arranged by size:
1753 1433 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 3779 1239
Size: 1001 1539 2579
Fragments arranged by size:
2579 1539 1001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2175 3244 4109 4407 2175
Size: 1069 865 298 2887
Fragments arranged by size:
2887 1069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2099 3782 882
Size: 617 600 1683 2219
Fragments arranged by size:
2219 1683 617 600
BsaAI yAC'GTr
Cuts at: 493 1204 2910 493
Size: 711 1706 2702
Fragments arranged by size:
2702 1706 711
BsaBI GATnn'nnATC
Cuts at: 5113 5113
Size: 5119
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2926 3308 3943 275
Size: 53 83 186 936 1393 382 635 1451
Fragments arranged by size:
1451 1393 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2079 2103 3552 3950 4224 4371 4782 2079
Size: 24 1449 398 274 147 411 2416
Fragments arranged by size:
2416 1449 411 398 274 147 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4894
Fragments arranged by size:
4894 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2071 1186
Size: 885 4234
BseYI C'CCAG_C
Cuts at: 2291 4322 4818 2291
Size: 2031 496 2592
Fragments arranged by size:
2592 2031 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 2128 1163
Size: 573 315 77 4154
Fragments arranged by size:
4154 573 315 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2569 3330 4358 1540
Size: 275 186 568 761 1028 2301
Fragments arranged by size:
2301 1028 761 568 275 186
BsiHKAI G_wGCw'C
Cuts at: 2157 2252 3180 3265 4336 2157
Size: 95 928 85 1071 2940
Fragments arranged by size:
2940 1071 928 95 85
BsmI GAATG_Cn'
Cuts at: 2630 2723 2630
Size: 93 5026
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2252 2438 3180 3265 4336 1391
Size: 399 367 95 186 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 367 186 95 85
BspEI T'CCGG_A
Cuts at: 2079 4782 2079
Size: 2703 2416
BspHI T'CATG_A
Cuts at: 3008 4738 3008
Size: 1730 3389
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2140 2474 844
Size: 1296 334 3489
Fragments arranged by size:
3489 1296 334
BsrBI CCG'CTC
Cuts at: 1394 1823 3006 1394
Size: 429 1183 3507
Fragments arranged by size:
3507 1183 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2257 2269 3614 3788 65
Size: 1183 1009 12 1345 174 1396
Fragments arranged by size:
1396 1345 1183 1009 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 3763 3950 1700
Size: 110 1953 187 2869
Fragments arranged by size:
2869 1953 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5119
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5119
BssSI C'ACGA_G
Cuts at: 3179 4191 3179
Size: 1012 4107
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5119
BstBI TT'CG_AA
Cuts at: 3923 3923
Size: 5119
BstEII G'GTnAC_C
Cuts at: 1184 2206 1184
Size: 1022 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 2218 2347 2218
Size: 129 4990
BstYI r'GATC_y
Cuts at: 1972 2449 2816 3201 3218 4670 4756 4768 5114
Size: 477 367 385 17 1452 86 12 346
Cuts at: 5114 1972
Size: 1977
Fragments arranged by size:
1977 1452 477 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2049 513
Size: 552 939 45 3583
Fragments arranged by size:
3583 939 552 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4380
Fragments arranged by size:
4380 683 56
BtsI GCAGTG_nn'
Cuts at: 2635 3429 3449 2635
Size: 794 20 4305
Fragments arranged by size:
4305 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5119
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5084
DraI TTT'AAA
Cuts at: 2423 2423
Size: 5119
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4126 809
Size: 507 2810 1802
Fragments arranged by size:
2810 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2566 3455 5084
Size: 54 1425 50 275 18 736 889 1629
Cuts at: 5084 8
Size: 43
Fragments arranged by size:
1629 1425 889 736 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2566 1537
Size: 275 754 4090
Fragments arranged by size:
4090 754 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3049 1353
Size: 1696 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3706 4080 4226 387
Size: 1064 138 2117 374 146 1280
Fragments arranged by size:
2117 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2291 3182 3779 4566 4834 1239
Size: 393 608 51 891 597 787 268 1524
Fragments arranged by size:
1524 891 787 608 597 393 268 51
EcoRI G'AATT_C
Cuts at: 2428 2806 2428
Size: 378 4741
FspI TGC'GCA
Cuts at: 3625 3625
Size: 5119
HaeII r_GCGC'y
Cuts at: 1678 2115 2173 4266 1678
Size: 437 58 2093 2531
Fragments arranged by size:
2531 2093 437 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2077 2093 2109 3760
Size: 32 135 32 489 16 16 16 1651
Cuts at: 3760 3792 3834 3866 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1651 489 135 42 32 32 32
32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2463 2709 669
Size: 988 281 525 246 3079
Fragments arranged by size:
3079 988 525 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5119
HpaI GTT'AAC
Cuts at: 2709 2709
Size: 5119
KpnI G_GTAC'C
Cuts at: 2444 2444
Size: 5119
MfeI C'AATT_G
Cuts at: 2409 2718 2409
Size: 309 4810
MluI A'CGCG_T
Cuts at: 4995 4995
Size: 5119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4233 4417 1305
Size: 2928 184 2007
Fragments arranged by size:
2928 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3297
Fragments arranged by size:
3297 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2262 3077 3436 3595 518
Size: 648 1096 815 359 159 2042
Fragments arranged by size:
2042 1096 815 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2323 2535 3212 4360 4605 1606
Size: 512 205 212 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 512 245 212 205
NcoI C'CATG_G
Cuts at: 513 1065 2004 2049 513
Size: 552 939 45 3583
Fragments arranged by size:
3583 939 552 45
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5119
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5119
NotI GC'GGCC_GC
Cuts at: 2566 2566
Size: 5119
NsiI A_TGCA'T
Cuts at: 2259 2259
Size: 5119
NspI r_CATG'y
Cuts at: 1132 1229 2362 2477 4022 1132
Size: 97 1133 115 1545 2229
Fragments arranged by size:
2229 1545 1133 115 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5119
PciI A'CATG_T
Cuts at: 4018 4018
Size: 5119
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2203 1108
Size: 1095 4024
PfoI T'CCnGG_A
Cuts at: 2229 2229
Size: 5119
PmeI GTTT'AAAC
Cuts at: 2423 2423
Size: 5119
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 4701 4733 3188
Size: 32 1481 32 3574
Fragments arranged by size:
3574 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5119
PsiI TTA'TAA
Cuts at: 2689 2689
Size: 5119
PstI C_TGCA'G
Cuts at: 830 2019 2471 830
Size: 1189 452 3478
Fragments arranged by size:
3478 1189 452
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5119
PvuII CAG'CTG
Cuts at: 2118 2323 2118
Size: 205 4914
SalI G'TCGA_C
Cuts at: 1936 2461 1936
Size: 525 4594
SbfI CC_TGCA'GG
Cuts at: 2471 2471
Size: 5119
ScaI AGT'ACT
Cuts at: 3367 5079 3367
Size: 1712 3407
SfcI C'TryA_G
Cuts at: 826 1045 2015 2467 3602 4283 4474 826
Size: 219 970 452 1135 681 191 1471
Fragments arranged by size:
1471 1135 970 681 452 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5119
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5119
SmaI CCC'GGG
Cuts at: 1872 2446 1872
Size: 574 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2190 3223 4124 4386 820
Size: 197 926 247 1033 901 262 1553
Fragments arranged by size:
1553 1033 926 901 262 247 197
SnaBI TAC'GTA
Cuts at: 493 2910 493
Size: 2417 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5119
SphI G_CATG'C
Cuts at: 1132 2477 1132
Size: 1345 3774
SspI AAT'ATT
Cuts at: 5 52 3043 5
Size: 47 2991 2081
Fragments arranged by size:
2991 2081 47
StuI AGG'CCT
Cuts at: 1517 2138 1517
Size: 621 4498
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2558 513
Size: 552 939 45 509 3074
Fragments arranged by size:
3074 939 552 509 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3146 3163 3316 3501 3946 325
Size: 1255 1566 17 153 185 445 1498
Fragments arranged by size:
1566 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3365 5077 96
Size: 274 80 33 51 1443 1388 1712 138
Fragments arranged by size:
1712 1443 1388 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5079 5111 5079
Size: 32 5087
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5119
XbaI T'CTAG_A
Cuts at: 2455 2455
Size: 5119
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5119
XmaI C'CCGG_G
Cuts at: 1870 2444 1870
Size: 574 4545
XmnI GAAnn'nnTTC
Cuts at: 3248 3248
Size: 5119
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2927 276
Size: 53 83 186 936 1393 2468
Fragments arranged by size:
2468 1393 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI