Restriction map for
pFN21AE1157
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BcnI BfrI
BglI BlnI BpmI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PstI PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
Tsp45I Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AclI 1160 AACGTT AA^CGTT
AflIII 1142 ACATGT A^C[GA][CT]GT
AseI 1435 ATTAAT AT^TAAT
AsnI 1435 ATTAAT AT^TAAT
Asp700I 883 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1280 GAGCAC G[AT]GC[AT]^C
BanII 711 GGGCTC G[GA]GC[CT]^C
BciVI 1482 GGATAC GTATCC(6/5)
BclI 786 TGATCA T^GATCA
BglII 327 AGATCT A^GATCT
BlpI 1177 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1177 GCTCAGC GC^T[ACGT]AGC
BsaI 1578 GAGACC GGTCTC(1/5)
BsaJI 590 CCCAGG C^C[ACGT][ACGT]GG
BseRI 502 GAGGAG GAGGAG(10/8)
BsiHKAI 1280 GAGCAC G[AT]GC[AT]^C
BsmFI 800 GGGAC GGGAC(10/14)
BsmI 1132 GCATTC GAATGC(1/-1)
BspLU11I 1142 ACATGT A^CATGT
BspMI 1581 ACCTGC ACCTGC(4/8)
BsrDI 1607 GCAATG GCAATG(2/0)
CelII 1177 GCTCAGC GC^T[ACGT]AGC
DraII 800 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 800 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 848 ATGCAT ATGCA^T
FbaI 786 TGATCA T^GATCA
HaeIII 1062 GGCC GG^CC
NdeI 1534 CATATG CA^TATG
NsiI 848 ATGCAT ATGCA^T
PpuMI 800 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 1435 ATTAAT AT^TAAT
Psp1406I 1160 AACGTT AA^CGTT
PvuII 233 CAGCTG CAG^CTG
SexAI 1386 ACCAGGT A^CC[AT]GGT
SfcI 483 CTGTAG C^T[GA][CT]AG
SmlI 581 CTCAAG C^T[CT][GA]AG
StuI 1061 AGGCCT AGG^CCT
XcmI 679 CCATCAGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 883 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 389 GTAC GT^AC
810 GTAC
BmyI 711 GGGCTC G[AGT]GC[ACT]^C
1280 GAGCAC
BsiYI 90 CCAGTGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1444 CCAACAGGTGG
BslI 90 CCAGTGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1444 CCAACAGGTGG
BsmAI 1578 GAGAC GTCTC(1/5)
1654 GTCTC
Bsp1286I 711 GGGCTC G[AGT]GC[ACT]^C
1280 GAGCAC
BspHI 249 TCATGA T^CATGA
357 TCATGA
BstYI 327 AGATCT [GA]^GATC[CT]
898 GGATCT
MflI 327 AGATCT [GA]^GATC[CT]
898 GGATCT
PflMI 90 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1444 CCAACAGGTGG
RcaI 249 TCATGA T^CATGA
357 TCATGA
RsaI 389 GTAC GT^AC
810 GTAC
ScaI 388 AGTACT AGT^ACT
809 AGTACT
SspI 620 AATATT AAT^ATT
1395 AATATT
Van91I 90 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1444 CCAACAGGTGG
XbaI 853 TCTAGA T^CTAGA
1248 TCTAGA
XhoII 327 AGATCT [GA]^GATC[CT]
898 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AvaII BbsI BbvI BfaI
BpuAI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I