(Linear) (Six-base) MAPSORT of: KIBB6601.seq Check: 9555 from: 1 to: 1221 KIBB6601.orf With 182 enzymes: SgfI * July 15, 2010 18:30 .. AccI GT'mk_AC Cuts at: 0 674 1221 Size: 674 547 Acc65I G'GTAC_C Cuts at: 0 244 1221 Size: 244 977 AfeI AGC'GCT Cuts at: 0 388 1221 Size: 388 833 AgeI A'CCGG_T Cuts at: 0 241 1221 Size: 241 980 AlwNI CAG_nnn'CTG Cuts at: 0 741 906 1221 Size: 741 165 315 Fragments arranged by size: 741 315 165 ApaI G_GGCC'C Cuts at: 0 29 1221 Size: 29 1192 ApoI r'AATT_y Cuts at: 0 1147 1221 Size: 1147 74 AvaI C'yCGr_G Cuts at: 0 534 707 1053 1085 1221 Size: 534 173 346 32 136 Fragments arranged by size: 534 346 173 136 32 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 236 269 381 414 1221 Size: 236 33 112 33 807 Fragments arranged by size: 807 236 112 33 33 BanI G'GyrC_C Cuts at: 0 244 562 1221 Size: 244 318 659 Fragments arranged by size: 659 318 244 BanII G_rGCy'C Cuts at: 0 29 832 1221 Size: 29 803 389 Fragments arranged by size: 803 389 29 BbeI G_GCGC'C Cuts at: 0 566 1221 Size: 566 655 BbsI GAAGACnn'nnnn_ Cuts at: 0 780 1221 Size: 780 441 BbvCI CC'TCA_GC Cuts at: 0 967 1077 1221 Size: 967 110 144 Fragments arranged by size: 967 144 110 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1054 1088 1221 Size: 1054 34 133 Fragments arranged by size: 1054 133 34 BglI GCCn_nnn'nGGC Cuts at: 0 376 481 1221 Size: 376 105 740 Fragments arranged by size: 740 376 105 BlpI GC'TnA_GC Cuts at: 0 734 1221 Size: 734 487 Bme1580I G_kGCm'C Cuts at: 0 29 1221 Size: 29 1192 BmgBI CAC'GTC Cuts at: 0 579 672 1221 Size: 579 93 549 Fragments arranged by size: 579 549 93 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1010 1221 Size: 1010 211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 940 1030 1221 Size: 76 864 90 191 Fragments arranged by size: 864 191 90 76 Bpu10I CC'TnA_GC Cuts at: 0 136 967 1077 1221 Size: 136 831 110 144 Fragments arranged by size: 831 144 136 110 BsaHI Gr'CG_yC Cuts at: 0 326 563 951 1221 Size: 326 237 388 270 Fragments arranged by size: 388 326 270 237 BsaWI w'CCGG_w Cuts at: 0 241 1221 Size: 241 980 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 163 193 1221 Size: 163 30 1028 Fragments arranged by size: 1028 163 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 73 171 1221 Size: 73 98 1050 Fragments arranged by size: 1050 98 73 BseYI C'CCAG_C Cuts at: 0 260 363 486 504 558 606 695 1003 Size: 260 103 123 18 54 48 89 308 Cuts at: 1003 1093 1162 1221 Size: 90 69 59 Fragments arranged by size: 308 260 123 103 90 89 69 59 54 48 18 BsiEI CG_ry'CG Cuts at: 0 428 476 623 1221 Size: 428 48 147 598 Fragments arranged by size: 598 428 147 48 BsiHKAI G_wGCw'C Cuts at: 0 947 1221 Size: 947 274 BsmBI CGTCTCn'nnnn_ Cuts at: 0 57 313 958 1221 Size: 57 256 645 263 Fragments arranged by size: 645 263 256 57 Bsp1286I G_dGCh'C Cuts at: 0 29 832 947 1221 Size: 29 803 115 274 Fragments arranged by size: 803 274 115 29 BspHI T'CATG_A Cuts at: 0 332 1221 Size: 332 889 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 134 1221 Size: 134 1087 BsrFI r'CCGG_y Cuts at: 0 241 1221 Size: 241 980 BssHII G'CGCG_C Cuts at: 0 152 1221 Size: 152 1069 BssSI C'ACGA_G Cuts at: 0 1070 1221 Size: 1070 151 BstEII G'GTnAC_C Cuts at: 0 237 1221 Size: 237 984 BstXI CCAn_nnnn'nTGG Cuts at: 0 129 1221 Size: 129 1092 BstYI r'GATC_y Cuts at: 0 902 1221 Size: 902 319 BtgI C'CryG_G Cuts at: 0 290 374 455 678 854 1221 Size: 290 84 81 223 176 367 Fragments arranged by size: 367 290 223 176 84 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 777 1221 Size: 777 444 BtsI GCAGTG_nn' Cuts at: 0 1035 1113 1221 Size: 1035 78 108 Fragments arranged by size: 1035 108 78 DraIII CAC_nnn'GTG Cuts at: 0 291 665 998 1221 Size: 291 374 333 223 Fragments arranged by size: 374 333 291 223 EaeI y'GGCC_r Cuts at: 0 473 656 758 887 1221 Size: 473 183 102 129 334 Fragments arranged by size: 473 334 183 129 102 EagI C'GGCC_G Cuts at: 0 473 1221 Size: 473 748 EarI CTCTTCn'nnn_ Cuts at: 0 280 601 1221 Size: 280 321 620 Fragments arranged by size: 620 321 280 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 922 1036 1221 Size: 922 114 185 Fragments arranged by size: 922 185 114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 940 1030 1221 Size: 76 864 90 191 Fragments arranged by size: 864 191 90 76 EcoNI CCTnn'n_nnAGG Cuts at: 0 1193 1221 Size: 1193 28 EcoO109I rG'GnC_Cy Cuts at: 0 25 26 539 1221 Size: 25 1 513 682 Fragments arranged by size: 682 513 25 1 HaeII r_GCGC'y Cuts at: 0 390 441 487 566 1221 Size: 390 51 46 79 655 Fragments arranged by size: 655 390 79 51 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 56 88 717 749 974 1006 1017 1049 Size: 56 32 629 32 225 32 11 32 Cuts at: 1049 1221 Size: 172 Fragments arranged by size: 629 225 172 56 32 32 32 32 11 KasI G'GCGC_C Cuts at: 0 562 1221 Size: 562 659 KpnI G_GTAC'C Cuts at: 0 248 1221 Size: 248 973 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 146 1216 1221 Size: 146 1070 5 Fragments arranged by size: 1070 146 5 MscI TGG'CCA Cuts at: 0 658 760 889 1221 Size: 658 102 129 332 Fragments arranged by size: 658 332 129 102 MslI CAynn'nnrTG Cuts at: 0 253 754 1221 Size: 253 501 467 Fragments arranged by size: 501 467 253 MspA1I CmG'CkG Cuts at: 0 357 843 1093 1221 Size: 357 486 250 128 Fragments arranged by size: 486 357 250 128 NarI GG'CG_CC Cuts at: 0 563 1221 Size: 563 658 NcoI C'CATG_G Cuts at: 0 374 1221 Size: 374 847 NotI GC'GGCC_GC Cuts at: 0 473 1221 Size: 473 748 NspI r_CATG'y Cuts at: 0 1134 1221 Size: 1134 87 PasI CC'CwG_GG Cuts at: 0 21 914 1221 Size: 21 893 307 Fragments arranged by size: 893 307 21 PflMI CCAn_nnn'nTGG Cuts at: 0 906 1221 Size: 906 315 PfoI T'CCnGG_A Cuts at: 0 905 1221 Size: 905 316 PpuMI rG'GwC_Cy Cuts at: 0 539 1221 Size: 539 682 PspOMI G'GGCC_C Cuts at: 0 25 1221 Size: 25 1196 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 913 945 1221 Size: 913 32 276 Fragments arranged by size: 913 276 32 PvuII CAG'CTG Cuts at: 0 843 1093 1221 Size: 843 250 128 Fragments arranged by size: 843 250 128 SapI GCTCTTCn'nnn_ Cuts at: 0 280 601 1221 Size: 280 321 620 Fragments arranged by size: 620 321 280 SfiI GGCCn_nnn'nGGCC Cuts at: 0 376 1221 Size: 376 845 SfoI GGC'GCC Cuts at: 0 564 1221 Size: 564 657 SmaI CCC'GGG Cuts at: 0 536 709 1055 1221 Size: 536 173 346 166 Fragments arranged by size: 536 346 173 166 SphI G_CATG'C Cuts at: 0 1134 1221 Size: 1134 87 StyI C'CwwG_G Cuts at: 0 374 1221 Size: 374 847 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 609 906 1221 Size: 609 297 315 Fragments arranged by size: 609 315 297 TatI w'GTAC_w Cuts at: 0 1013 1221 Size: 1013 208 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 163 195 1221 Size: 163 32 1026 Fragments arranged by size: 1026 163 32 Tth111I GACn'n_nGTC Cuts at: 0 986 1221 Size: 986 235 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 459 1221 Size: 459 762 XmaI C'CCGG_G Cuts at: 0 534 707 1053 1221 Size: 534 173 346 168 Fragments arranged by size: 534 346 173 168 Enzymes that do cut: AccI Acc65I AfeI AgeI AlwNI ApaI ApoI AvaI BaeI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BssHII BssSI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NotI NspI PasI PflMI PfoI PpuMI PspOMI PsrI PvuII SapI SfiI SfoI SmaI SphI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AclI AcuI AflII AflIII AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BamHI BciVI BclI BfrBI BglII BmrI BmtI BpuEI BsaI BsaAI BsaBI BsgI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI