################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BbsI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NciI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI SexAI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 715 GACGTC GACGT^C AccI 468 GTCGAC GT^[AC][GT]AC AgeI 1280 ACCGGT A^CCGGT Alw44I 622 GTGCAC G^TGCAC AlwNI 473 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 622 GTGCAC G^TGCAC Asp700I 172 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 1015 GGATCC G^GATCC BanII 214 GAGCTC G[GA]GC[CT]^C BbrPI 1204 CACGTG CAC^GTG BsaAI 1204 CACGTG [CT]AC^GT[GA] BsaI 322 GAGACC GGTCTC(1/5) BspMI 783 ACCTGC ACCTGC(4/8) BsrBI 1186 GAGCGG CCGCTC(-3/-3) BsrDI 617 GCAATG GCAATG(2/0) BssSI 593 CTCGTG CACGAG(-5/-1) BstAPI 1310 GCACTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 990 CCAACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1015 GGATCC [GA]^GATC[CT] DraII 282 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 1311 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 960 CCGTGG C^C[GA][CT]GG EarI 694 CTCTTC CTCTTC(1/4) EcoO109I 282 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 1273 GAATTC G^AATTC EcoT14I 200 CCTTGG C^C[AT][AT]GG EcoT22I 94 ATGCAT ATGCA^T HgaI 68 GACGC GACGC(5/10) Ksp632I 694 CTCTTC CTCTTC(1/4) MflI 1015 GGATCC [GA]^GATC[CT] MseI 1223 TTAA T^TAA NheI 138 GCTAGC G^CTAGC NsiI 94 ATGCAT ATGCA^T PflMI 246 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1280 ACCGGT A^CCGGT PmaCI 1204 CACGTG CAC^GTG PmlI 1204 CACGTG CAC^GTG PpuMI 282 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 409 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 214 GAGCTC GAGCT^C SalI 468 GTCGAC G^TCGAC ScaI 679 AGTACT AGT^ACT SgrAI 1279 CACCGGTG C[GA]^CCGG[CT]G SphI 92 GCATGC GCATG^C StuI 953 AGGCCT AGG^CCT StyI 200 CCTTGG C^C[AT][AT]GG Tru9I 1223 TTAA T^TAA Van91I 246 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1015 GGATCC [GA]^GATC[CT] XmnI 172 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 15 CGCG CG^CG 1079 CGCG AspHI 214 GAGCTC G[AT]GC[AT]^C 622 GTGCAC BanI 6 GGCGCC G^G[CT][GA]CC 841 GGCGCC BbeI 6 GGCGCC GGCGC^C 841 GGCGCC BbvI 1307 GCAGC GCAGC(8/12) 270 GCTGC BcgI 1161 CGAGGCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 817 GCACTCCTCTCG BmyI 214 GAGCTC G[AGT]GC[ACT]^C 622 GTGCAC BsaWI 426 TCCGGT [AT]^CCGG[AT] 1280 ACCGGT BsgI 740 CTGCAC GTGCAG(16/14) 785 CTGCAC BsiHKAI 214 GAGCTC G[AT]GC[AT]^C 622 GTGCAC Bsp1286I 214 GAGCTC G[AGT]GC[ACT]^C 622 GTGCAC BsrFI 1042 GCCGGT [GA]^CCGG[CT] 1280 ACCGGT BstUI 15 CGCG CG^CG 1079 CGCG Cfr10I 1042 GCCGGT [GA]^CCGG[CT] 1280 ACCGGT EaeI 53 TGGCCG [CT]^GGCC[GA] 1208 TGGCCG FauI 16 GCGGG CCCGC(4/6) 1188 GCGGG HaeII 6 GGCGCC [GA]GCGC^[CT] 841 GGCGCC HincII 468 GTCGAC GT[CT]^[GA]AC 985 GTTGAC HindII 468 GTCGAC GT[CT]^[GA]AC 985 GTTGAC HindIII 290 AAGCTT A^AGCTT 700 AAGCTT KasI 6 GGCGCC G^GCGCC 841 GGCGCC MaeII 716 ACGT A^CGT 1205 ACGT MvnI 15 CGCG CG^CG 1079 CGCG NarI 6 GGCGCC GG^CGCC 841 GGCGCC PstI 763 CTGCAG CTGCA^G 1305 CTGCAG PvuII 148 CAGCTG CAG^CTG 565 CAGCTG SfoI 6 GGCGCC GGC^GCC 841 GGCGCC SmlI 411 CTTGAG C^T[CT][GA]AG 1172 CTCAAG TaqI 469 TCGA T^CGA 1160 TCGA TseI 270 GCTGC G^C[AT]GC 1307 GCAGC TthHB8I 469 TCGA T^CGA 1160 TCGA XcmI 845 CCATCTATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 951 CCAGGCCTGCCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI AvaII BfaI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tsp45I Tsp509I TspRI