(Circular) (Six-base) MAPSORT of: pFN21AE1915.seq Check: 6481 from: 1 to: 6061 pFN21AE1915 6061 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2724 3871 278 Size: 53 83 186 936 1188 1147 2468 Fragments arranged by size: 2468 1188 1147 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2474 3404 1937 Size: 537 930 4594 Fragments arranged by size: 4594 930 537 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2363 2510 4124 5508 5776 1632 Size: 731 147 1614 1384 268 1917 Fragments arranged by size: 1917 1614 1384 731 268 147 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 3285 4892 3285 Size: 1607 4454 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2793 1184 Size: 1609 4452 AlwNI CAG_nnn'CTG Cuts at: 2483 3804 5376 2483 Size: 1321 1572 3168 Fragments arranged by size: 3168 1572 1321 ApaLI G'TGCA_C Cuts at: 2627 4118 5274 2627 Size: 1491 1156 3414 Fragments arranged by size: 3414 1491 1156 ApoI r'AATT_y Cuts at: 1493 2549 2654 2773 3278 3370 3596 3748 5857 Size: 1056 105 119 505 92 226 152 2109 Cuts at: 5857 1493 Size: 1697 Fragments arranged by size: 2109 1697 1056 505 226 152 119 105 92 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 3386 1870 Size: 73 1443 4545 Fragments arranged by size: 4545 1443 73 BamHI G'GATC_C Cuts at: 3020 3391 3758 3020 Size: 371 367 5323 Fragments arranged by size: 5323 371 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2846 3382 4837 618 Size: 325 217 627 224 835 536 1455 1842 Fragments arranged by size: 1842 1455 835 627 536 325 224 217 BanII G_rGCy'C Cuts at: 1391 2223 3380 1391 Size: 832 1157 4072 Fragments arranged by size: 4072 1157 832 BbeI G_GCGC'C Cuts at: 2015 2850 2015 Size: 835 5226 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5090 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2811 2845 3155 3189 4252 4286 1903 Size: 34 874 34 310 34 1063 34 3678 Fragments arranged by size: 3678 1063 874 310 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BfrBI ATG'CAT Cuts at: 2101 2101 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4672 136 Size: 107 122 71 631 605 337 2663 1525 Fragments arranged by size: 2663 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 2631 4122 5278 1790 Size: 841 1491 1156 2573 Fragments arranged by size: 2573 1491 1156 841 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3792 4750 442 Size: 416 53 83 399 24 2375 958 1753 Fragments arranged by size: 2375 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2147 1056 Size: 1091 4970 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2366 2658 2685 2861 4721 1239 Size: 968 159 292 27 176 1860 2579 Fragments arranged by size: 2579 1860 968 292 176 159 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2437 3162 4186 5051 5349 2437 Size: 725 1024 865 298 3149 Fragments arranged by size: 3149 1024 865 725 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2321 4724 882 Size: 617 822 2403 2219 Fragments arranged by size: 2403 2219 822 617 BsaAI yAC'GTr Cuts at: 493 1204 3211 3852 493 Size: 711 2007 641 2702 Fragments arranged by size: 2702 2007 711 641 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2721 2847 3868 Size: 53 83 186 936 479 709 126 1021 Cuts at: 3868 4250 4885 275 Size: 382 635 1451 Fragments arranged by size: 1451 1021 936 709 635 479 382 186 126 83 53 BsaWI w'CCGG_w Cuts at: 2431 3285 4494 4892 5166 5313 5724 2431 Size: 854 1209 398 274 147 411 2768 Fragments arranged by size: 2768 1209 854 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2339 2369 2658 2688 1178 Size: 30 165 30 936 30 289 30 4551 Fragments arranged by size: 4551 936 289 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2816 3050 1186 Size: 1069 561 234 4197 Fragments arranged by size: 4197 1069 561 234 BseYI C'CCAG_C Cuts at: 5264 5760 5264 Size: 496 5565 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2730 2775 1163 Size: 573 994 45 4449 Fragments arranged by size: 4449 994 573 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 2631 4122 4207 5278 2223 Size: 408 1491 85 1071 3006 Fragments arranged by size: 3006 1491 1071 408 85 BsmI GAATG_Cn' Cuts at: 3572 3665 3572 Size: 93 5968 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2631 3380 4122 4207 5278 1391 Size: 399 433 408 749 742 85 1071 2174 Fragments arranged by size: 2174 1071 749 742 433 408 399 85 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2797 3416 844 Size: 1953 619 3489 Fragments arranged by size: 3489 1953 619 BsrBI CCG'CTC Cuts at: 1394 1823 3193 3948 1394 Size: 429 1370 755 3507 Fragments arranged by size: 3507 1370 755 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2629 4556 4730 65 Size: 1183 1381 1927 174 1396 Fragments arranged by size: 1927 1396 1381 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3047 3285 4705 4892 1700 Size: 110 1237 238 1420 187 2869 Fragments arranged by size: 2869 1420 1237 238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6061 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 2598 4121 5133 2598 Size: 1523 1012 3526 Fragments arranged by size: 3526 1523 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3321 794 Size: 2527 3534 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 3002 3002 Size: 6061 BstYI r'GATC_y Cuts at: 1972 3020 3391 3758 4143 4160 5612 5698 5710 Size: 1048 371 367 385 17 1452 86 12 Cuts at: 5710 6056 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1048 385 371 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2965 513 Size: 552 939 961 3609 Fragments arranged by size: 3609 961 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 3577 4371 4391 3577 Size: 794 20 5247 Fragments arranged by size: 5247 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DraIII CAC_nnn'GTG Cuts at: 3321 3321 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 3213 3508 Size: 54 1425 50 275 18 228 1155 295 Cuts at: 3508 4397 6026 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1155 889 295 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2705 3991 1353 Size: 1352 1286 3423 Fragments arranged by size: 3423 1352 1286 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2456 4648 5022 5168 387 Size: 1064 138 867 2192 374 146 1280 Fragments arranged by size: 2192 1280 1064 867 374 146 138 EcoICRI GAG'CTC Cuts at: 2221 2221 Size: 6061 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2363 2366 2510 2658 2685 2861 Size: 393 575 156 3 144 148 27 176 Cuts at: 2861 4124 4721 5508 5776 1239 Size: 1263 597 787 268 1524 Fragments arranged by size: 1524 1263 787 597 575 393 268 176 156 148 144 27 3 EcoO109I rG'GnC_Cy Cuts at: 2288 2288 Size: 6061 EcoRI G'AATT_C Cuts at: 3278 3370 3748 3278 Size: 92 378 5591 Fragments arranged by size: 5591 378 92 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 2015 2850 5208 1678 Size: 337 835 2358 2531 Fragments arranged by size: 2531 2358 835 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4702 4734 4776 4808 1373 Size: 32 135 32 3130 32 42 32 2626 Fragments arranged by size: 3130 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2475 2992 3405 3651 669 Size: 988 281 537 517 413 246 3079 Fragments arranged by size: 3079 988 537 517 413 281 246 HindIII A'AGCT_T Cuts at: 748 2295 2705 748 Size: 1547 410 4104 Fragments arranged by size: 4104 1547 410 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KasI G'GCGC_C Cuts at: 2011 2846 2011 Size: 835 5226 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5175 5359 1305 Size: 3870 184 2007 Fragments arranged by size: 3870 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4239 Fragments arranged by size: 4239 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4019 4378 4537 518 Size: 648 2853 359 159 2042 Fragments arranged by size: 2853 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2155 2572 3477 4154 5302 5547 1606 Size: 457 92 417 905 677 1148 245 2120 Fragments arranged by size: 2120 1148 905 677 457 417 245 92 NarI GG'CG_CC Cuts at: 2012 2847 2012 Size: 835 5226 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4570 Fragments arranged by size: 4570 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NheI G'CTAG_C Cuts at: 1052 2143 1052 Size: 1091 4970 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NsiI A_TGCA'T Cuts at: 2103 2103 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 2101 2387 3223 3419 4964 1132 Size: 97 872 286 836 196 1545 2229 Fragments arranged by size: 2229 1545 872 836 286 196 97 PasI CC'CwG_GG Cuts at: 1348 3026 1348 Size: 1678 4383 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 1108 Size: 1149 4912 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PmlI CAC'GTG Cuts at: 3211 3211 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2288 2288 Size: 6061 PshAI GACnn'nnGTC Cuts at: 1613 2418 1613 Size: 805 5256 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PstI C_TGCA'G Cuts at: 830 2772 3314 3413 830 Size: 1942 542 99 3478 Fragments arranged by size: 3478 1942 542 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 2155 2572 2155 Size: 417 5644 SacI G_AGCT'C Cuts at: 2223 2223 Size: 6061 SalI G'TCGA_C Cuts at: 1936 2473 3403 1936 Size: 537 930 4594 Fragments arranged by size: 4594 930 537 SanDI GG'GwC_CC Cuts at: 2288 2288 Size: 6061 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 2686 4309 6021 2686 Size: 1623 1712 2726 Fragments arranged by size: 2726 1712 1623 SfcI C'TryA_G Cuts at: 826 1045 2768 2927 3310 3409 4544 5225 5416 Size: 219 1723 159 383 99 1135 681 191 Cuts at: 5416 826 Size: 1471 Fragments arranged by size: 1723 1471 1135 681 383 219 191 159 99 SfoI GGC'GCC Cuts at: 2013 2848 2013 Size: 835 5226 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 3285 1700 Size: 1585 4476 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2416 3177 4165 5066 5328 820 Size: 197 926 473 761 988 901 262 1553 Fragments arranged by size: 1553 988 926 901 761 473 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 2101 3419 1132 Size: 969 1318 3774 Fragments arranged by size: 3774 1318 969 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 2960 1517 Size: 1443 4618 StyI C'CwwG_G Cuts at: 513 1065 2004 2205 3500 513 Size: 552 939 201 1295 3074 Fragments arranged by size: 3074 1295 939 552 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3018 4088 4105 4258 4443 4888 325 Size: 1255 1438 1070 17 153 185 445 1498 Fragments arranged by size: 1498 1438 1255 1070 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2386 2684 4307 Size: 274 80 33 51 1443 409 298 1623 Cuts at: 4307 6019 96 Size: 1712 138 Fragments arranged by size: 1712 1623 1443 409 298 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2658 2690 3005 3037 6021 6053 2658 Size: 32 315 32 2984 32 2666 Fragments arranged by size: 2984 2666 315 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2857 2963 2857 Size: 106 5955 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 2181 4190 2181 Size: 2009 4052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2722 3869 276 Size: 53 83 186 936 1188 1147 2468 Fragments arranged by size: 2468 1188 1147 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI