(Linear) (Six-base) MAPSORT of: KIEE1915.seq Check: 9393 from: 1 to: 1356 KIEE1915.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AatII G_ACGT'C Cuts at: 0 719 1356 Size: 719 637 AccI GT'mk_AC Cuts at: 0 469 1356 Size: 469 887 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 505 1356 Size: 358 147 851 Fragments arranged by size: 851 358 147 AgeI A'CCGG_T Cuts at: 0 1280 1356 Size: 1280 76 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 788 1356 Size: 788 568 AlwNI CAG_nnn'CTG Cuts at: 0 478 1356 Size: 478 878 ApaLI G'TGCA_C Cuts at: 0 622 1356 Size: 622 734 ApoI r'AATT_y Cuts at: 0 544 649 768 1273 1356 Size: 544 105 119 505 83 Fragments arranged by size: 544 505 119 105 83 BamHI G'GATC_C Cuts at: 0 1015 1356 Size: 1015 341 BanI G'GyrC_C Cuts at: 0 6 841 1356 Size: 6 835 515 Fragments arranged by size: 835 515 6 BanII G_rGCy'C Cuts at: 0 218 1356 Size: 218 1138 BbeI G_GCGC'C Cuts at: 0 10 845 1356 Size: 10 835 511 Fragments arranged by size: 835 511 10 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 806 840 1150 1184 1356 Size: 806 34 310 34 172 Fragments arranged by size: 806 310 172 34 34 BfrBI ATG'CAT Cuts at: 0 96 1356 Size: 96 1260 Bme1580I G_kGCm'C Cuts at: 0 626 1356 Size: 626 730 BmtI G_CTAG'C Cuts at: 0 142 1356 Size: 142 1214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 361 653 680 856 1356 Size: 202 159 292 27 176 500 Fragments arranged by size: 500 292 202 176 159 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 432 1157 1356 Size: 432 725 199 Fragments arranged by size: 725 432 199 BsaI GGTCTCn'nnnn_ Cuts at: 0 316 1356 Size: 316 1040 BsaAI yAC'GTr Cuts at: 0 1206 1356 Size: 1206 150 BsaHI Gr'CG_yC Cuts at: 0 7 716 842 1356 Size: 7 709 126 514 Fragments arranged by size: 709 514 126 7 BsaWI w'CCGG_w Cuts at: 0 426 1280 1356 Size: 426 854 76 Fragments arranged by size: 854 426 76 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 334 364 653 683 1356 Size: 334 30 289 30 673 Fragments arranged by size: 673 334 289 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 250 811 1045 1356 Size: 250 561 234 311 Fragments arranged by size: 561 311 250 234 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 725 770 1356 Size: 725 45 586 Fragments arranged by size: 725 586 45 BsiHKAI G_wGCw'C Cuts at: 0 218 626 1356 Size: 218 408 730 Fragments arranged by size: 730 408 218 Bsp1286I G_dGCh'C Cuts at: 0 218 626 1356 Size: 218 408 730 Fragments arranged by size: 730 408 218 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 792 1356 Size: 792 564 BsrBI CCG'CTC Cuts at: 0 1188 1356 Size: 1188 168 BsrDI GCAATG_nn' Cuts at: 0 624 1356 Size: 624 732 BsrFI r'CCGG_y Cuts at: 0 1042 1280 1356 Size: 1042 238 76 Fragments arranged by size: 1042 238 76 BssSI C'ACGA_G Cuts at: 0 593 1356 Size: 593 763 BstAPI GCAn_nnn'nTGC Cuts at: 0 1316 1356 Size: 1316 40 BstXI CCAn_nnnn'nTGG Cuts at: 0 997 1356 Size: 997 359 BstYI r'GATC_y Cuts at: 0 1015 1356 Size: 1015 341 BtgI C'CryG_G Cuts at: 0 960 1356 Size: 960 396 DraIII CAC_nnn'GTG Cuts at: 0 1316 1356 Size: 1316 40 EaeI y'GGCC_r Cuts at: 0 53 1208 1356 Size: 53 1155 148 Fragments arranged by size: 1155 148 53 EarI CTCTTCn'nnn_ Cuts at: 0 700 1356 Size: 700 656 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 451 1356 Size: 451 905 EcoICRI GAG'CTC Cuts at: 0 216 1356 Size: 216 1140 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 358 361 505 653 680 856 1356 Size: 202 156 3 144 148 27 176 500 Fragments arranged by size: 500 202 176 156 148 144 27 3 EcoO109I rG'GnC_Cy Cuts at: 0 283 1356 Size: 283 1073 EcoRI G'AATT_C Cuts at: 0 1273 1356 Size: 1273 83 HaeII r_GCGC'y Cuts at: 0 10 845 1356 Size: 10 835 511 Fragments arranged by size: 835 511 10 HincII GTy'rAC Cuts at: 0 470 987 1356 Size: 470 517 369 Fragments arranged by size: 517 470 369 HindIII A'AGCT_T Cuts at: 0 290 700 1356 Size: 290 410 656 Fragments arranged by size: 656 410 290 KasI G'GCGC_C Cuts at: 0 6 841 1356 Size: 6 835 515 Fragments arranged by size: 835 515 6 MspA1I CmG'CkG Cuts at: 0 58 150 567 1356 Size: 58 92 417 789 Fragments arranged by size: 789 417 92 58 NarI GG'CG_CC Cuts at: 0 7 842 1356 Size: 7 835 514 Fragments arranged by size: 835 514 7 NheI G'CTAG_C Cuts at: 0 138 1356 Size: 138 1218 NsiI A_TGCA'T Cuts at: 0 98 1356 Size: 98 1258 NspI r_CATG'y Cuts at: 0 96 382 1218 1356 Size: 96 286 836 138 Fragments arranged by size: 836 286 138 96 PasI CC'CwG_GG Cuts at: 0 1021 1356 Size: 1021 335 PflMI CCAn_nnn'nTGG Cuts at: 0 252 1356 Size: 252 1104 PmlI CAC'GTG Cuts at: 0 1206 1356 Size: 1206 150 PpuMI rG'GwC_Cy Cuts at: 0 283 1356 Size: 283 1073 PshAI GACnn'nnGTC Cuts at: 0 413 1356 Size: 413 943 PstI C_TGCA'G Cuts at: 0 767 1309 1356 Size: 767 542 47 Fragments arranged by size: 767 542 47 PvuII CAG'CTG Cuts at: 0 150 567 1356 Size: 150 417 789 Fragments arranged by size: 789 417 150 SacI G_AGCT'C Cuts at: 0 218 1356 Size: 218 1138 SalI G'TCGA_C Cuts at: 0 468 1356 Size: 468 888 SanDI GG'GwC_CC Cuts at: 0 283 1356 Size: 283 1073 ScaI AGT'ACT Cuts at: 0 681 1356 Size: 681 675 SfcI C'TryA_G Cuts at: 0 763 922 1305 1356 Size: 763 159 383 51 Fragments arranged by size: 763 383 159 51 SfoI GGC'GCC Cuts at: 0 8 843 1356 Size: 8 835 513 Fragments arranged by size: 835 513 8 SgrAI Cr'CCGG_yG Cuts at: 0 1280 1356 Size: 1280 76 SmlI C'TyrA_G Cuts at: 0 411 1172 1356 Size: 411 761 184 Fragments arranged by size: 761 411 184 SphI G_CATG'C Cuts at: 0 96 1356 Size: 96 1260 StuI AGG'CCT Cuts at: 0 955 1356 Size: 955 401 StyI C'CwwG_G Cuts at: 0 200 1356 Size: 200 1156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1013 1356 Size: 1013 343 TatI w'GTAC_w Cuts at: 0 381 679 1356 Size: 381 298 677 Fragments arranged by size: 677 381 298 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 653 685 1000 1032 1356 Size: 653 32 315 32 324 Fragments arranged by size: 653 324 315 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 852 958 1356 Size: 852 106 398 Fragments arranged by size: 852 398 106 XmnI GAAnn'nnTTC Cuts at: 0 176 1356 Size: 176 1180 ZraI GAC'GTC Cuts at: 0 717 1356 Size: 717 639 Enzymes that do cut: AatII AccI AcuI AgeI AlfI AlwNI ApaLI ApoI BamHI BanI BanII BbeI BcgI BfrBI Bme1580I BmtI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII HincII HindIII KasI MspA1I NarI NheI NsiI NspI PasI PflMI PmlI PpuMI PshAI PstI PvuII SacI SalI SanDI ScaI SfcI SfoI SgrAI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BbsI BbvCI BciVI BclI BglI BglII BlpI BmgBI BmrI BplI Bpu10I BsaBI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI EcoNI EcoRV FalI FseI FspI FspAI Hin4I HpaI KpnI MfeI MluI MmeI MscI MslI NaeI NcoI NdeI NgoMIV NotI NruI PacI PciI PfoI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SapI SbfI SexAI SfiI SgfI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI