################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AclI AcsI AflII AhdI AocI Aor51HI ApoI AscI AseI AsnI AspEI AvrII BamHI BbrPI BbvCI BclI BfrI BglII BlnI BsaBI BsiEI BsiWI BsmI BspDI BspHI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SalI SapI SbfI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp509I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 515 GGTACC G^GTACC AgeI 512 ACCGGT A^CCGGT Alw44I 1533 GTGCAC G^TGCAC ApaLI 1533 GTGCAC G^TGCAC Asp700I 912 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 515 GGTACC G^GTACC AviII 1249 TGCGCA TGC^GCA BalI 1943 TGGCCA TGG^CCA BcgI 158 GCACCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 765 GGATAC GTATCC(6/5) BfaI 451 CTAG C^TAG BlpI 1677 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1677 GCTCAGC GC^T[ACGT]AGC BsaAI 1006 TACGTG [CT]AC^GT[GA] BsmBI 1243 CGTCTC CGTCTC(1/5) Bsp1407I 14 TGTACA T^GTACA BsrGI 14 TGTACA T^GTACA BstAPI 300 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1677 GCTCAGC GC^T[ACGT]AGC CpoI 213 CGGACCG CG^G[AT]CCG EarI 250 GAAGAG CTCTTC(1/4) EcoNI 882 CCTTTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 1249 TGCGCA TGC^GCA KpnI 515 GGTACC GGTAC^C Ksp632I 250 GAAGAG CTCTTC(1/4) KspI 2056 CCGCGG CCGC^GG MaeI 451 CTAG C^TAG MluNI 1943 TGGCCA TGG^CCA MscI 1943 TGGCCA TGG^CCA MslI 1851 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 2009 GCCGGC GCC^GGC NcoI 761 CCATGG C^CATGG NgoMIV 2009 GCCGGC G^CCGGC PinAI 512 ACCGGT A^CCGGT PpuMI 1221 GGGTCCC [GA]G^G[AT]CC[CT] RsrII 213 CGGACCG CG^G[AT]CCG SacI 85 GAGCTC GAGCT^C SacII 2056 CCGCGG CCGC^GG ScaI 974 AGTACT AGT^ACT SfiI 1518 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 14 TGTACA T^GTACA Tsp45I 382 GTCAC ^GT[GC]AC XmnI 912 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 34 GTCTAC GT^[AC][GT]AC 454 GTCTAC AccIII 71 TCCGGA T^CCGGA 267 TCCGGA AlwNI 634 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 2044 CAGGCCCTG ApaI 674 GGGCCC GGGCC^C 829 GGGCCC AspI 28 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC 399 GACCGAGTC AvaI 286 CCCGAG C^[CT]CG[GA]G 737 CCCGAG BbeI 1143 GGCGCC GGCGC^C 1448 GGCGCC BseAI 71 TCCGGA T^CCGGA 267 TCCGGA BsoBI 286 CCCGAG C^[CT]CG[GA]G 737 CCCGAG BspEI 71 TCCGGA T^CCGGA 267 TCCGGA BspLU11I 11 ACATGT A^CATGT 983 ACATGT BsrBI 651 CCGCTC CCGCTC(-3/-3) 424 GAGCGG BstYI 1463 AGATCC [GA]^GATC[CT] 1964 AGATCC DdeI 1040 CTGAG C^T[ACGT]AG 1678 CTCAG HincII 1105 GTCAAC GT[CT]^[GA]AC 2080 GTCAAC HindII 1105 GTCAAC GT[CT]^[GA]AC 2080 GTCAAC KasI 1143 GGCGCC G^GCGCC 1448 GGCGCC MflI 1463 AGATCC [GA]^GATC[CT] 1964 AGATCC MroI 71 TCCGGA T^CCGGA 267 TCCGGA MseI 541 TTAA T^TAA 1051 TTAA NarI 1143 GGCGCC GG^CGCC 1448 GGCGCC PflFI 28 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC 399 GACCGAGTC PflMI 633 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 756 CCACGCCATGG PstI 684 CTGCAG CTGCA^G 840 CTGCAG SfcI 684 CTGCAG C^T[GA][CT]AG 840 CTGCAG SfoI 1143 GGCGCC GGC^GCC 1448 GGCGCC SmlI 409 CTCAAG C^T[CT][GA]AG 1621 CTTGAG Tru9I 541 TTAA T^TAA 1051 TTAA TspRI 1207 CAGTG CA[GC]TG[ACGT][ACGT]^ 1237 CAGTG Tth111I 28 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC 399 GACCGAGTC Van91I 633 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 756 CCACGCCATGG XcmI 101 CCAATGGGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 803 CCATCTCGGCGCTGG XhoII 1463 AGATCC [GA]^GATC[CT] 1964 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AspHI AvaII BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI TthHB8I