(Circular) (Six-base) MAPSORT of: pFN21AE1183.seq Check: 284 from: 1 to: 6814 pFN21AE1183 6814 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4084 4084 Size: 6814 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4624 278 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2040 2460 4157 1937 Size: 103 420 1697 4594 Fragments arranged by size: 4594 1697 420 103 Acc65I G'GTAC_C Cuts at: 2520 4135 2520 Size: 1615 5199 AclI AA'CG_TT Cuts at: 1563 4941 5314 1563 Size: 3378 373 3063 Fragments arranged by size: 3378 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3060 3602 4877 6261 6529 1632 Size: 1428 542 1275 1384 268 1917 Fragments arranged by size: 1917 1428 1384 1275 542 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6617 AflIII A'CryG_T Cuts at: 1203 2016 2988 3936 5713 6690 1203 Size: 813 972 948 1777 977 1327 Fragments arranged by size: 1777 1327 977 972 948 813 AgeI A'CCGG_T Cuts at: 2517 5645 2517 Size: 3128 3686 AhdI GACnn_n'nnGTC Cuts at: 5543 5543 Size: 6814 AleI CACnn'nnGTG Cuts at: 1166 3860 1166 Size: 2694 4120 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6814 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3923 3955 3923 Size: 32 6782 AlwNI CAG_nnn'CTG Cuts at: 2644 4054 4557 6129 2644 Size: 1410 503 1572 3329 Fragments arranged by size: 3329 1572 1410 503 ApaI G_GGCC'C Cuts at: 2683 2838 2683 Size: 155 6659 ApaLI G'TGCA_C Cuts at: 3538 4871 6027 3538 Size: 1333 1156 4325 Fragments arranged by size: 4325 1333 1156 ApoI r'AATT_y Cuts at: 1493 4123 4349 4501 6610 1493 Size: 2630 226 152 2109 1697 Fragments arranged by size: 2630 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5368 160 Size: 872 4336 1606 Fragments arranged by size: 4336 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 AvaI C'yCGr_G Cuts at: 1870 1943 2291 2742 4139 1870 Size: 73 348 451 1397 4545 Fragments arranged by size: 4545 1397 451 348 73 BamHI G'GATC_C Cuts at: 4144 4511 4144 Size: 367 6447 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2520 2666 2821 3148 3453 Size: 325 217 627 733 146 155 327 305 Cuts at: 3453 3495 4135 5590 618 Size: 42 640 1455 1842 Fragments arranged by size: 1842 1455 733 640 627 327 325 305 217 155 146 42 BanII G_rGCy'C Cuts at: 1391 2094 2683 2838 3164 3386 3572 3884 4098 Size: 703 589 155 326 222 186 312 214 Cuts at: 4098 4133 1391 Size: 35 4072 Fragments arranged by size: 4072 703 589 326 312 222 214 186 155 35 BbeI G_GCGC'C Cuts at: 3152 3457 3152 Size: 305 6509 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2483 3002 3407 928 Size: 971 584 519 405 4335 Fragments arranged by size: 4335 971 584 519 405 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2152 2186 5005 5039 1903 Size: 34 215 34 2819 34 3678 Fragments arranged by size: 3678 2819 215 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2764 4706 5916 2764 Size: 1942 1210 3662 Fragments arranged by size: 3662 1942 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6814 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2813 3530 3707 Size: 107 122 71 631 605 1141 717 177 Cuts at: 3707 4063 5425 136 Size: 356 1362 1525 Fragments arranged by size: 1525 1362 1141 717 631 605 356 177 122 107 71 BglII A'GATC_T Cuts at: 6809 6809 Size: 6814 BlpI GC'TnA_GC Cuts at: 3683 4231 3683 Size: 548 6266 Bme1580I G_kGCm'C Cuts at: 1790 2669 2683 2838 3542 4875 6031 1790 Size: 879 14 155 704 1333 1156 2573 Fragments arranged by size: 2573 1333 1156 879 704 155 14 BmgBI CAC'GTC Cuts at: 1318 3937 1318 Size: 2619 4195 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3887 4545 5503 Size: 416 53 83 399 24 2470 658 958 Cuts at: 5503 442 Size: 1753 Fragments arranged by size: 2470 1753 958 658 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6814 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2071 2071 Size: 6814 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2041 2143 2366 2447 5474 1239 Size: 802 102 223 81 3027 2579 Fragments arranged by size: 3027 2579 802 223 102 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2399 3647 4939 5804 6102 2399 Size: 1248 1292 865 298 3111 Fragments arranged by size: 3111 1292 1248 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2396 2565 2582 3623 3953 5477 882 Size: 617 897 169 17 1041 330 1524 2219 Fragments arranged by size: 2219 1524 1041 897 617 330 169 17 BsaAI yAC'GTr Cuts at: 493 1204 3013 4605 493 Size: 711 1809 1592 2702 Fragments arranged by size: 2702 1809 1592 711 BsaBI GATnn'nnATC Cuts at: 6808 6808 Size: 6814 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3149 3454 3695 3924 Size: 53 83 186 936 1616 305 241 229 Cuts at: 3924 4621 5003 5638 275 Size: 697 382 635 1451 Fragments arranged by size: 1616 1451 936 697 635 382 305 241 229 186 83 53 BsaWI w'CCGG_w Cuts at: 2076 2142 2272 2517 5247 5645 5919 6066 6477 Size: 66 130 245 2730 398 274 147 411 Cuts at: 6477 2076 Size: 2413 Fragments arranged by size: 2730 2413 411 398 274 245 147 130 66 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2022 2052 2783 2813 2852 Size: 30 165 30 619 30 731 30 39 Cuts at: 2852 2882 3713 3743 1178 Size: 30 831 30 4249 Fragments arranged by size: 4249 831 731 619 165 39 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2044 2102 2573 2876 3971 1186 Size: 858 58 471 303 1095 4029 Fragments arranged by size: 4029 1095 858 471 303 58 BseYI C'CCAG_C Cuts at: 2711 3364 6017 6513 2711 Size: 653 2653 496 3012 Fragments arranged by size: 3012 2653 653 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2401 2702 3035 3407 1163 Size: 573 665 301 333 372 4570 Fragments arranged by size: 4570 665 573 372 333 301 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4264 5025 6053 1540 Size: 275 186 2263 761 1028 2301 Fragments arranged by size: 2301 2263 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2094 2844 3542 3684 4875 4960 6031 2094 Size: 750 698 142 1191 85 1071 2877 Fragments arranged by size: 2877 1191 1071 750 698 142 85 BsmI GAATG_Cn' Cuts at: 4325 4418 4325 Size: 93 6721 BsmBI CGTCTCn'nnnn_ Cuts at: 3254 3254 Size: 6814 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2669 2683 2838 2844 3164 3386 Size: 399 304 575 14 155 6 320 222 Cuts at: 3386 3542 3572 3684 3884 4098 4133 4875 4960 Size: 156 30 112 200 214 35 742 85 Cuts at: 4960 6031 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 575 399 320 304 222 214 200 156 155 112 85 35 30 14 6 BspEI T'CCGG_A Cuts at: 2076 2272 6477 2076 Size: 196 4205 2413 Fragments arranged by size: 4205 2413 196 BspHI T'CATG_A Cuts at: 4703 6433 4703 Size: 1730 5084 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2295 3513 4084 4169 844 Size: 1451 1218 571 85 3489 Fragments arranged by size: 3489 1451 1218 571 85 BsrBI CCG'CTC Cuts at: 1394 1823 2431 2658 4701 1394 Size: 429 608 227 2043 3507 Fragments arranged by size: 3507 2043 608 429 227 BsrDI GCAATG_nn' Cuts at: 65 1248 5309 5483 65 Size: 1183 4061 174 1396 Fragments arranged by size: 4061 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2517 3125 4014 5458 5645 1700 Size: 110 707 608 889 1444 187 2869 Fragments arranged by size: 2869 1444 889 707 608 187 110 BsrGI T'GTAC_A Cuts at: 96 2019 96 Size: 1923 4891 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6814 BssSI C'ACGA_G Cuts at: 4874 5886 4874 Size: 1012 5802 BstAPI GCAn_nnn'nTGC Cuts at: 794 2311 794 Size: 1517 5297 BstBI TT'CG_AA Cuts at: 5618 5618 Size: 6814 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6814 BstXI CCAn_nnnn'nTGG Cuts at: 3164 3742 3811 3164 Size: 578 69 6167 Fragments arranged by size: 6167 578 69 BstYI r'GATC_y Cuts at: 1972 3468 3969 4144 4511 4896 4913 6365 6451 Size: 1496 501 175 367 385 17 1452 86 Cuts at: 6451 6463 6809 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1496 1452 501 385 367 346 175 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2766 2951 3355 3479 3793 4061 Size: 552 939 762 185 404 124 314 268 Cuts at: 4061 513 Size: 3266 Fragments arranged by size: 3266 939 762 552 404 314 268 185 124 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2155 4015 487 Size: 683 56 929 1860 3286 Fragments arranged by size: 3286 1860 929 683 56 BtsI GCAGTG_nn' Cuts at: 3248 4330 5124 5144 3248 Size: 1082 794 20 4918 Fragments arranged by size: 4918 1082 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6814 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6779 DraI TTT'AAA Cuts at: 4118 4118 Size: 6814 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5821 809 Size: 507 4505 1802 Fragments arranged by size: 4505 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2103 2178 Size: 54 1425 50 275 18 177 96 75 Cuts at: 2178 2566 3172 3522 3948 4064 4261 5150 6779 Size: 388 606 350 426 116 197 889 1629 Cuts at: 6779 8 Size: 43 Fragments arranged by size: 1629 1425 889 606 426 388 350 275 197 177 116 96 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4261 1537 Size: 275 2449 4090 Fragments arranged by size: 4090 2449 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 4744 1353 Size: 897 2494 3423 Fragments arranged by size: 3423 2494 897 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2243 3922 5401 5775 5921 387 Size: 1064 138 654 1679 1479 374 146 1280 Fragments arranged by size: 1679 1479 1280 1064 654 374 146 138 EcoICRI GAG'CTC Cuts at: 2092 2092 Size: 6814 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 2143 2366 2447 3060 3602 4877 Size: 393 409 102 223 81 613 542 1275 Cuts at: 4877 5474 6261 6529 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1275 787 613 597 542 409 393 268 223 102 81 EcoNI CCTnn'n_nnAGG Cuts at: 2891 2891 Size: 6814 EcoO109I rG'GnC_Cy Cuts at: 2680 3227 4051 2680 Size: 547 824 5443 Fragments arranged by size: 5443 824 547 EcoRI G'AATT_C Cuts at: 4123 4501 4123 Size: 378 6436 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3632 3632 Size: 6814 FspI TGC'GCA Cuts at: 3256 5320 3256 Size: 2064 4750 HaeII r_GCGC'y Cuts at: 1678 2730 2819 3152 3457 5961 1678 Size: 1052 89 333 305 2504 2531 Fragments arranged by size: 2531 2504 1052 333 305 89 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2393 2425 2852 Size: 32 135 32 450 32 339 32 427 Cuts at: 2852 2884 5455 5487 5529 5561 1373 Size: 32 2571 32 42 32 2626 Fragments arranged by size: 2626 2571 450 427 339 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3112 4087 4158 4404 669 Size: 988 281 1174 975 71 246 3079 Fragments arranged by size: 3079 1174 988 975 281 246 71 HindIII A'AGCT_T Cuts at: 748 748 Size: 6814 HpaI GTT'AAC Cuts at: 4404 4404 Size: 6814 KasI G'GCGC_C Cuts at: 3148 3453 3148 Size: 305 6509 KpnI G_GTAC'C Cuts at: 2524 4139 2524 Size: 1615 5199 MfeI C'AATT_G Cuts at: 4413 4413 Size: 6814 MluI A'CGCG_T Cuts at: 6690 6690 Size: 6814 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2008 2565 2728 3245 5928 6112 1305 Size: 703 557 163 517 2683 184 2007 Fragments arranged by size: 2683 2007 703 557 517 184 163 MscI TGG'CCA Cuts at: 10 64 1489 1832 3950 10 Size: 54 1425 343 2118 2874 Fragments arranged by size: 2874 2118 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3860 4772 5131 5290 518 Size: 648 2694 912 359 159 2042 Fragments arranged by size: 2694 2042 912 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2311 2344 2735 3038 3378 3574 3604 4063 Size: 705 33 391 303 340 196 30 459 Cuts at: 4063 4230 4907 6055 6300 1606 Size: 167 677 1148 245 2120 Fragments arranged by size: 2120 1148 705 677 459 391 340 303 245 196 167 33 30 NaeI GCC'GGC Cuts at: 4016 4016 Size: 6814 NarI GG'CG_CC Cuts at: 3149 3454 3149 Size: 305 6509 NcoI C'CATG_G Cuts at: 513 1065 2004 2766 513 Size: 552 939 762 4561 Fragments arranged by size: 4561 939 762 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6814 NgoMIV G'CCGG_C Cuts at: 4014 4014 Size: 6814 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6814 NotI GC'GGCC_GC Cuts at: 4261 4261 Size: 6814 NspI r_CATG'y Cuts at: 1132 1229 2020 2992 3241 4172 5717 1132 Size: 97 791 972 249 931 1545 2229 Fragments arranged by size: 2229 1545 972 931 791 249 97 PasI CC'CwG_GG Cuts at: 1348 3164 1348 Size: 1816 4998 PciI A'CATG_T Cuts at: 2016 2988 5713 2016 Size: 972 2725 3117 Fragments arranged by size: 3117 2725 972 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 2767 1108 Size: 1536 123 5155 Fragments arranged by size: 5155 1536 123 PmeI GTTT'AAAC Cuts at: 4118 4118 Size: 6814 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4883 4915 6396 6428 4883 Size: 32 1481 32 5269 Fragments arranged by size: 5269 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3227 3227 Size: 6814 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6814 PsiI TTA'TAA Cuts at: 4384 4384 Size: 6814 PspOMI G'GGCC_C Cuts at: 2679 2834 2679 Size: 155 6659 PstI C_TGCA'G Cuts at: 830 2693 2849 4166 830 Size: 1863 156 1317 3478 Fragments arranged by size: 3478 1863 1317 156 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6814 PvuII CAG'CTG Cuts at: 2311 2344 2735 3038 2311 Size: 33 391 303 6087 Fragments arranged by size: 6087 391 303 33 RsrII CG'GwC_CG Cuts at: 2219 2219 Size: 6814 SacI G_AGCT'C Cuts at: 2094 2094 Size: 6814 SacII CC_GC'GG Cuts at: 4064 4064 Size: 6814 SalI G'TCGA_C Cuts at: 1936 4156 1936 Size: 2220 4594 SanDI GG'GwC_CC Cuts at: 3227 3227 Size: 6814 SbfI CC_TGCA'GG Cuts at: 4166 4166 Size: 6814 ScaI AGT'ACT Cuts at: 2981 5062 6774 2981 Size: 2081 1712 3021 Fragments arranged by size: 3021 2081 1712 SfcI C'TryA_G Cuts at: 826 1045 2689 2845 4162 5297 5978 6169 826 Size: 219 1644 156 1317 1135 681 191 1471 Fragments arranged by size: 1644 1471 1317 1135 681 219 191 156 SfiI GGCCn_nnn'nGGCC Cuts at: 3530 3530 Size: 6814 SfoI GGC'GCC Cuts at: 3150 3455 3150 Size: 305 6509 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6814 SmaI CCC'GGG Cuts at: 1872 4141 1872 Size: 2269 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2414 3626 4918 5819 6081 820 Size: 197 926 471 1212 1292 901 262 1553 Fragments arranged by size: 1553 1292 1212 926 901 471 262 197 SnaBI TAC'GTA Cuts at: 493 4605 493 Size: 4112 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6814 SphI G_CATG'C Cuts at: 1132 4172 1132 Size: 3040 3774 SspI AAT'ATT Cuts at: 5 52 4738 5 Size: 47 4686 2081 Fragments arranged by size: 4686 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6814 StyI C'CwwG_G Cuts at: 513 1065 2004 2766 2802 3702 4253 513 Size: 552 939 762 36 900 551 3074 Fragments arranged by size: 3074 939 900 762 552 551 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2420 4841 4858 5011 5196 5641 325 Size: 1255 840 2421 17 153 185 445 1498 Fragments arranged by size: 2421 1498 1255 840 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2019 2979 5060 Size: 274 80 33 51 1443 42 960 2081 Cuts at: 5060 6772 96 Size: 1712 138 Fragments arranged by size: 2081 1712 1443 960 274 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6774 6806 6774 Size: 32 6782 Tth111I GACn'n_nGTC Cuts at: 1316 2036 2407 1316 Size: 720 371 5723 Fragments arranged by size: 5723 720 371 XbaI T'CTAG_A Cuts at: 4150 4150 Size: 6814 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2113 2815 2113 Size: 702 6112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6814 XmaI C'CCGG_G Cuts at: 1870 4139 1870 Size: 2269 4545 XmnI GAAnn'nnTTC Cuts at: 2921 4943 2921 Size: 2022 4792 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4622 276 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI SapI SexAI SrfI SwaI