(Linear) (Six-base) MAPSORT of: KIEE1183.seq Check: 2039 from: 1 to: 2109 KIEE1183.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2079 2109 Size: 2079 30 AccI GT'mk_AC Cuts at: 0 35 455 2109 Size: 35 420 1654 Fragments arranged by size: 1654 420 35 Acc65I G'GTAC_C Cuts at: 0 515 2109 Size: 515 1594 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1055 1597 2109 Size: 1055 542 512 Fragments arranged by size: 1055 542 512 AflIII A'CryG_T Cuts at: 0 11 983 1931 2109 Size: 11 972 948 178 Fragments arranged by size: 972 948 178 11 AgeI A'CCGG_T Cuts at: 0 512 2109 Size: 512 1597 AleI CACnn'nnGTG Cuts at: 0 1855 2109 Size: 1855 254 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1918 1950 2109 Size: 1918 32 159 Fragments arranged by size: 1918 159 32 AlwNI CAG_nnn'CTG Cuts at: 0 639 2049 2109 Size: 639 1410 60 Fragments arranged by size: 1410 639 60 ApaI G_GGCC'C Cuts at: 0 678 833 2109 Size: 678 155 1276 Fragments arranged by size: 1276 678 155 ApaLI G'TGCA_C Cuts at: 0 1533 2109 Size: 1533 576 AvaI C'yCGr_G Cuts at: 0 286 737 2109 Size: 286 451 1372 Fragments arranged by size: 1372 451 286 BanI G'GyrC_C Cuts at: 0 515 661 816 1143 1448 1490 2109 Size: 515 146 155 327 305 42 619 Fragments arranged by size: 619 515 327 305 155 146 42 BanII G_rGCy'C Cuts at: 0 89 678 833 1159 1381 1567 1879 2093 Size: 89 589 155 326 222 186 312 214 Cuts at: 2093 2109 Size: 16 Fragments arranged by size: 589 326 312 222 214 186 155 89 16 BbeI G_GCGC'C Cuts at: 0 1147 1452 2109 Size: 1147 305 657 Fragments arranged by size: 1147 657 305 BbsI GAAGACnn'nnnn_ Cuts at: 0 478 997 1402 2109 Size: 478 519 405 707 Fragments arranged by size: 707 519 478 405 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 147 181 2109 Size: 147 34 1928 Fragments arranged by size: 1928 147 34 BciVI GTATCCnnnnn_n' Cuts at: 0 759 2109 Size: 759 1350 BglI GCCn_nnn'nGGC Cuts at: 0 808 1525 1702 2058 2109 Size: 808 717 177 356 51 Fragments arranged by size: 808 717 356 177 51 BlpI GC'TnA_GC Cuts at: 0 1678 2109 Size: 1678 431 Bme1580I G_kGCm'C Cuts at: 0 664 678 833 1537 2109 Size: 664 14 155 704 572 Fragments arranged by size: 704 664 572 155 14 BmgBI CAC'GTC Cuts at: 0 1932 2109 Size: 1932 177 BmrI ACTGGGnnnn_n' Cuts at: 0 1882 2109 Size: 1882 227 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 66 2109 Size: 66 2043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 138 361 442 2109 Size: 36 102 223 81 1667 Fragments arranged by size: 1667 223 102 81 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 1642 2109 Size: 394 1248 467 Fragments arranged by size: 1248 467 394 BsaI GGTCTCn'nnnn_ Cuts at: 0 391 560 577 1618 1948 2109 Size: 391 169 17 1041 330 161 Fragments arranged by size: 1041 391 330 169 161 17 BsaAI yAC'GTr Cuts at: 0 1008 2109 Size: 1008 1101 BsaHI Gr'CG_yC Cuts at: 0 1144 1449 1690 1919 2109 Size: 1144 305 241 229 190 Fragments arranged by size: 1144 305 241 229 190 BsaWI w'CCGG_w Cuts at: 0 71 137 267 512 2109 Size: 71 66 130 245 1597 Fragments arranged by size: 1597 245 130 71 66 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 17 47 778 808 847 877 1708 1738 Size: 17 30 731 30 39 30 831 30 Cuts at: 1738 2109 Size: 371 Fragments arranged by size: 831 731 371 39 30 30 30 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 39 97 568 871 1966 2109 Size: 39 58 471 303 1095 143 Fragments arranged by size: 1095 471 303 143 58 39 BseYI C'CCAG_C Cuts at: 0 706 1359 2109 Size: 706 653 750 Fragments arranged by size: 750 706 653 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 396 697 1030 1402 2109 Size: 396 301 333 372 707 Fragments arranged by size: 707 396 372 333 301 BsiHKAI G_wGCw'C Cuts at: 0 89 839 1537 1679 2109 Size: 89 750 698 142 430 Fragments arranged by size: 750 698 430 142 89 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1249 2109 Size: 1249 860 Bsp1286I G_dGCh'C Cuts at: 0 89 664 678 833 839 1159 1381 1537 Size: 89 575 14 155 6 320 222 156 Cuts at: 1537 1567 1679 1879 2093 2109 Size: 30 112 200 214 16 Fragments arranged by size: 575 320 222 214 200 156 155 112 89 30 16 14 6 BspEI T'CCGG_A Cuts at: 0 71 267 2109 Size: 71 196 1842 Fragments arranged by size: 1842 196 71 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 290 1508 2079 2109 Size: 290 1218 571 30 Fragments arranged by size: 1218 571 290 30 BsrBI CCG'CTC Cuts at: 0 426 653 2109 Size: 426 227 1456 Fragments arranged by size: 1456 426 227 BsrFI r'CCGG_y Cuts at: 0 512 1120 2009 2109 Size: 512 608 889 100 Fragments arranged by size: 889 608 512 100 BsrGI T'GTAC_A Cuts at: 0 14 2109 Size: 14 2095 BstAPI GCAn_nnn'nTGC Cuts at: 0 306 2109 Size: 306 1803 BstXI CCAn_nnnn'nTGG Cuts at: 0 1159 1737 1806 2109 Size: 1159 578 69 303 Fragments arranged by size: 1159 578 303 69 BstYI r'GATC_y Cuts at: 0 1463 1964 2109 Size: 1463 501 145 Fragments arranged by size: 1463 501 145 BtgI C'CryG_G Cuts at: 0 761 946 1350 1474 1788 2056 2109 Size: 761 185 404 124 314 268 53 Fragments arranged by size: 761 404 314 268 185 124 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 150 2010 2109 Size: 150 1860 99 Fragments arranged by size: 1860 150 99 BtsI GCAGTG_nn' Cuts at: 0 1243 2109 Size: 1243 866 EaeI y'GGCC_r Cuts at: 0 2 98 173 561 1167 1517 1943 2059 Size: 2 96 75 388 606 350 426 116 Cuts at: 2059 2109 Size: 50 Fragments arranged by size: 606 426 388 350 116 96 75 50 2 EarI CTCTTCn'nnn_ Cuts at: 0 245 2109 Size: 245 1864 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 238 1917 2109 Size: 238 1679 192 Fragments arranged by size: 1679 238 192 EcoICRI GAG'CTC Cuts at: 0 87 2109 Size: 87 2022 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 138 361 442 1055 1597 2109 Size: 36 102 223 81 613 542 512 Fragments arranged by size: 613 542 512 223 102 81 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 886 2109 Size: 886 1223 EcoO109I rG'GnC_Cy Cuts at: 0 675 1222 2046 2109 Size: 675 547 824 63 Fragments arranged by size: 824 675 547 63 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1627 2109 Size: 1627 482 FspI TGC'GCA Cuts at: 0 1251 2109 Size: 1251 858 HaeII r_GCGC'y Cuts at: 0 725 814 1147 1452 2109 Size: 725 89 333 305 657 Fragments arranged by size: 725 657 333 305 89 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 17 49 388 420 847 879 2109 Size: 17 32 339 32 427 32 1230 Fragments arranged by size: 1230 427 339 32 32 32 17 HincII GTy'rAC Cuts at: 0 1107 2082 2109 Size: 1107 975 27 Fragments arranged by size: 1107 975 27 KasI G'GCGC_C Cuts at: 0 1143 1448 2109 Size: 1143 305 661 Fragments arranged by size: 1143 661 305 KpnI G_GTAC'C Cuts at: 0 519 2109 Size: 519 1590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3 560 723 1240 2109 Size: 3 557 163 517 869 Fragments arranged by size: 869 557 517 163 3 MscI TGG'CCA Cuts at: 0 1945 2109 Size: 1945 164 MslI CAynn'nnrTG Cuts at: 0 1855 2109 Size: 1855 254 MspA1I CmG'CkG Cuts at: 0 306 339 730 1033 1373 1569 1599 2058 Size: 306 33 391 303 340 196 30 459 Cuts at: 2058 2109 Size: 51 Fragments arranged by size: 459 391 340 306 303 196 51 33 30 NaeI GCC'GGC Cuts at: 0 2011 2109 Size: 2011 98 NarI GG'CG_CC Cuts at: 0 1144 1449 2109 Size: 1144 305 660 Fragments arranged by size: 1144 660 305 NcoI C'CATG_G Cuts at: 0 761 2109 Size: 761 1348 NgoMIV G'CCGG_C Cuts at: 0 2009 2109 Size: 2009 100 NspI r_CATG'y Cuts at: 0 15 987 1236 2109 Size: 15 972 249 873 Fragments arranged by size: 972 873 249 15 PasI CC'CwG_GG Cuts at: 0 1159 2109 Size: 1159 950 PciI A'CATG_T Cuts at: 0 11 983 2109 Size: 11 972 1126 Fragments arranged by size: 1126 972 11 PflMI CCAn_nnn'nTGG Cuts at: 0 639 762 2109 Size: 639 123 1347 Fragments arranged by size: 1347 639 123 PpuMI rG'GwC_Cy Cuts at: 0 1222 2109 Size: 1222 887 PspOMI G'GGCC_C Cuts at: 0 674 829 2109 Size: 674 155 1280 Fragments arranged by size: 1280 674 155 PstI C_TGCA'G Cuts at: 0 688 844 2109 Size: 688 156 1265 Fragments arranged by size: 1265 688 156 PvuII CAG'CTG Cuts at: 0 306 339 730 1033 2109 Size: 306 33 391 303 1076 Fragments arranged by size: 1076 391 306 303 33 RsrII CG'GwC_CG Cuts at: 0 214 2109 Size: 214 1895 SacI G_AGCT'C Cuts at: 0 89 2109 Size: 89 2020 SacII CC_GC'GG Cuts at: 0 2059 2109 Size: 2059 50 SanDI GG'GwC_CC Cuts at: 0 1222 2109 Size: 1222 887 ScaI AGT'ACT Cuts at: 0 976 2109 Size: 976 1133 SfcI C'TryA_G Cuts at: 0 684 840 2109 Size: 684 156 1269 Fragments arranged by size: 1269 684 156 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1525 2109 Size: 1525 584 SfoI GGC'GCC Cuts at: 0 1145 1450 2109 Size: 1145 305 659 Fragments arranged by size: 1145 659 305 SmlI C'TyrA_G Cuts at: 0 409 1621 2109 Size: 409 1212 488 Fragments arranged by size: 1212 488 409 StyI C'CwwG_G Cuts at: 0 761 797 1697 2109 Size: 761 36 900 412 Fragments arranged by size: 900 761 412 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 415 2109 Size: 415 1694 TatI w'GTAC_w Cuts at: 0 14 974 2109 Size: 14 960 1135 Fragments arranged by size: 1135 960 14 Tth111I GACn'n_nGTC Cuts at: 0 31 402 2109 Size: 31 371 1707 Fragments arranged by size: 1707 371 31 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 108 810 2109 Size: 108 702 1299 Fragments arranged by size: 1299 702 108 XmnI GAAnn'nnTTC Cuts at: 0 916 2109 Size: 916 1193 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AgeI AleI AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PpuMI PspOMI PstI PvuII RsrII SacI SacII SanDI ScaI SfcI SfiI SfoI SmlI StyI TaqII TatI Tth111I XcmI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AhdI AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BclI BfrBI BglII BmtI Bpu10I BsaBI BsiEI BsiWI BsmI BspHI BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI FspAI HindIII HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI SalI SapI SbfI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XhoI XmaI ZraI