################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII Cfr10I CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HindIII HpaI KasI KpnI MaeI MamI MflI MluI MluNI MroI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcsI 826 GAATTC [GA]^AATT[CT] AlwNI 85 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 826 GAATTC [GA]^AATT[CT] BanI 360 GGTGCC G^G[CT][GA]CC BanII 187 GAGCTC G[GA]GC[CT]^C BcnI 20 CCCGG CC^[GC]GG BsmAI 1083 GTCTC GTCTC(1/5) BsmI 1041 GAATGC GAATGC(1/-1) BspDI 922 ATCGAT AT^CGAT BspMI 846 GCAGGT ACCTGC(4/8) BsrBI 547 CCGCTC CCGCTC(-3/-3) ClaI 922 ATCGAT AT^CGAT DraII 647 AGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 328 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 725 CCGCGG C^C[GA][CT]GG EaeI 236 TGGCCG [CT]^GGCC[GA] EcoNI 661 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 647 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 826 GAATTC G^AATTC HincII 850 GTCAAC GT[CT]^[GA]AC HindII 850 GTCAAC GT[CT]^[GA]AC HphI 1098 GGTGA GGTGA(8/7) KspI 725 CCGCGG CCGC^GG MaeII 206 ACGT A^CGT MfeI 674 CAATTG C^AATTG MunI 674 CAATTG C^AATTG NciI 20 CCCGG CC^[GC]GG NruI 125 TCGCGA TCG^CGA PshAI 201 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1021 CTGCAG CTGCA^G PvuII 148 CAGCTG CAG^CTG SacI 187 GAGCTC GAGCT^C SacII 725 CCGCGG CCGC^GG ScaI 631 AGTACT AGT^ACT TaqI 923 TCGA T^CGA TthHB8I 923 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 3 GGCGTC G[GA]^CG[CT]C 535 GACGCC AocI 364 CCTCAGG CC^T[ACGT]AGG 760 CCTGAGG AvaII 392 GGACC G^G[AT]CC 903 GGACC BsaHI 3 GGCGTC G[GA]^CG[CT]C 535 GACGCC BsaWI 174 TCCGGT [AT]^CCGG[AT] 738 ACCGGA BstF5I 885 GGATG GGATG(2/0) 975 GGATG Bsu36I 364 CCTCAGG CC^T[ACGT]AGG 760 CCTGAGG DpnII 802 GATC ^GATC 921 GATC Eco81I 364 CCTCAGG CC^T[ACGT]AGG 760 CCTGAGG EcoT14I 356 CCTTGG C^C[AT][AT]GG 644 CCAAGG FokI 885 GGATG GGATG(9/13) 975 GGATG HaeII 288 AGCGCC [GA]GCGC^[CT] 312 AGCGCC Hin1I 3 GGCGTC G[GA]^CG[CT]C 535 GACGCC MboI 802 GATC ^GATC 921 GATC MslI 354 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 383 CATCTGCGTG MspA1I 148 CAGCTG C[AC]G^C[GT]G 725 CCGCGG NdeII 802 GATC ^GATC 921 GATC NlaIII 694 CATG CATG^ 840 CATG NlaIV 360 GGTGCC GG[ACGT]^[ACGT]CC 648 GGCCCC Sau3AI 802 GATC ^GATC 921 GATC SfaNI 769 GATGC GCATC(5/9) 790 GATGC SfcI 1021 CTGCAG C^T[GA][CT]AG 1069 CTACAG StyI 356 CCTTGG C^C[AT][AT]GG 644 CCAAGG TfiI 115 GAATC G^A[AT]TC 966 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AccII AciI AfaI AluI AspHI BbvI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI EarI EcoRII Fnu4HI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeIII MboII MnlI MseI MspI MvaI MvnI MwoI PleI RsaI Sau96I ScrFI Tru9I TseI Tsp509I