################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI ItaI KasI KpnI KspI MaeII MamI MflI MluI MroI MspA1I MvnI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TseI Tth111I Van91I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AciI 262 GCGG CCGC(-3/-1) AflIII 517 ACATGT A^C[GA][CT]GT AgeI 1376 ACCGGT A^CCGGT Alw44I 277 GTGCAC G^TGCAC ApaLI 277 GTGCAC G^TGCAC Asp700I 414 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 277 GTGCAC G[AT]GC[AT]^C BalI 160 TGGCCA TGG^CCA BanII 85 GAGCCC G[GA]GC[CT]^C BbsI 25 GTCTTC GAAGAC(2/6) BclI 59 TGATCA T^GATCA BglI 1201 GCCTTTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 25 GTCTTC GAAGAC(2/6) BsaJI 30 CCTGGG C^C[ACGT][ACGT]GG BsaWI 1376 ACCGGT [AT]^CCGG[AT] BsiHKAI 277 GTGCAC G[AT]GC[AT]^C BsmI 1210 GCATTC GAATGC(1/-1) BspLU11I 517 ACATGT A^CATGT BsrFI 1376 ACCGGT [GA]^CCGG[CT] BstAPI 270 GCATAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 1376 ACCGGT [GA]^CCGG[CT] DpnII 60 GATC ^GATC DraI 1036 TTTAAA TTT^AAA EaeI 160 TGGCCA [CT]^GGCC[GA] FbaI 59 TGATCA T^GATCA HaeIII 161 GGCC GG^CC HapII 1377 CCGG C^CGG HpaII 1377 CCGG C^CGG HphI 1501 GGTGA GGTGA(8/7) MboI 60 GATC ^GATC MfeI 759 CAATTG C^AATTG MluNI 160 TGGCCA TGG^CCA MscI 160 TGGCCA TGG^CCA MspI 1377 CCGG C^CGG MunI 759 CAATTG C^AATTG NdeI 1495 CATATG CA^TATG NdeII 60 GATC ^GATC PinAI 1376 ACCGGT A^CCGGT Sau3AI 60 GATC ^GATC SexAI 20 ACCTGGT A^CC[AT]GGT SfcI 12 CTACAG C^T[GA][CT]AG SspI 333 AATATT AAT^ATT TaqI 1395 TCGA T^CGA TthHB8I 1395 TCGA T^CGA XbaI 1477 TCTAGA T^CTAGA XmnI 414 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AluI 948 AGCT AG^CT 1368 AGCT BmyI 85 GAGCCC G[AGT]GC[ACT]^C 277 GTGCAC BsmAI 102 GAGAC GTCTC(1/5) 43 GTCTC Bsp1286I 85 GAGCCC G[AGT]GC[ACT]^C 277 GTGCAC BsrDI 848 GCAATG GCAATG(2/0) 1268 GCAATG BssKI 21 CCTGG ^CC[ACGT]GG 30 CCTGG BstF5I 571 CATCC GGATG(2/0) 264 GGATG BstNI 21 CCTGG CC^[AT]GG 30 CCTGG EarI 99 GAAGAG CTCTTC(1/4) 661 GAAGAG EcoRI 902 GAATTC G^AATTC 1322 GAATTC EcoRII 21 CCTGG ^CC[AT]GG 30 CCTGG FokI 571 CATCC GGATG(9/13) 264 GGATG HindIII 947 AAGCTT A^AGCTT 1367 AAGCTT HinfI 181 GATTC G^A[ACGT]TC 582 GAATC Ksp632I 99 GAAGAG CTCTTC(1/4) 661 GAAGAG MaeIII 1184 GTGAC ^GT[ACGT]AC 1445 GTGAC MslI 1091 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1495 CATATGGGTG MvaI 21 CCTGG CC^[AT]GG 30 CCTGG NspI 105 ACATGC [GA]CATG^[CT] 517 ACATGT ScrFI 21 CCTGG CC^[ACGT]GG 30 CCTGG TfiI 181 GATTC G^A[AT]TC 582 GAATC Tsp45I 1184 GTGAC ^GT[GC]AC 1445 GTGAC TspRI 1197 CAGTG CA[GC]TG[ACGT][ACGT]^ 1449 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AcsI ApoI AvaII BfaI BpmI BsiYI BslI BsrI Cfr13I DdeI MaeI MboII MnlI MseI MwoI NlaIII Sau96I Tru9I Tsp509I