################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BssSI Bst1107I BstAPI BstBI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FseI FspI HindIII HinfI HpaI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 496 GTCGAC GT^[AC][GT]AC AcsI 358 GAATTC [GA]^AATT[CT] AflIII 212 ACGTGT A^C[GA][CT]GT AlwI 458 GGATC GGATC(4/5) AlwNI 556 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 336 CCTCAGG CC^T[ACGT]AGG ApoI 358 GAATTC [GA]^AATT[CT] AvaII 246 GGACC G^G[AT]CC BanI 40 GGCGCC G^G[CT][GA]CC BbeI 40 GGCGCC GGCGC^C BbsI 601 GTCTTC GAAGAC(2/6) BciVI 473 GTATCC GTATCC(6/5) BclI 350 TGATCA T^GATCA BpmI 85 CTGGAG CTGGAG(16/14) BpuAI 601 GTCTTC GAAGAC(2/6) BsaI 618 GGTCTC GGTCTC(1/5) BsiEI 498 CGACCG CG[GA][CT]^CG BsiYI 47 CCGGAGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 47 CCGGAGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 197 GGGAC GGGAC(10/14) Bsp1407I 628 TGTACA T^GTACA BspMI 101 ACCTGC ACCTGC(4/8) BsrDI 448 CATTGC GCAATG(2/0) BsrFI 500 ACCGGC [GA]^CCGG[CT] BsrGI 628 TGTACA T^GTACA Bsu36I 336 CCTCAGG CC^T[ACGT]AGG Cfr10I 500 ACCGGC [GA]^CCGG[CT] DdeI 337 CTCAG C^T[ACGT]AG Eco81I 336 CCTCAGG CC^T[ACGT]AGG EcoRI 358 GAATTC G^AATTC EcoT14I 77 CCTTGG C^C[AT][AT]GG FauI 509 CCCGC CCCGC(4/6) FbaI 350 TGATCA T^GATCA HgaI 256 GCGTC GACGC(5/10) HincII 496 GTCGAC GT[CT]^[GA]AC HindII 496 GTCGAC GT[CT]^[GA]AC KasI 40 GGCGCC G^GCGCC MaeII 212 ACGT A^CGT MslI 576 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 142 CAGCTG C[AC]G^C[GT]G NarI 40 GGCGCC GG^CGCC NdeI 380 CATATG CA^TATG NlaIII 177 CATG CATG^ PvuII 142 CAGCTG CAG^CTG SalI 496 GTCGAC G^TCGAC SapI 322 GAAGAGC GCTCTTC(1/4) ScaI 485 AGTACT AGT^ACT SfaNI 569 GCATC GCATC(5/9) SfoI 40 GGCGCC GGC^GCC SspBI 628 TGTACA T^GTACA StyI 77 CCTTGG C^C[AT][AT]GG TaqI 497 TCGA T^CGA TspRI 67 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 497 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 40 GGCGCC G[GA]^CG[CT]C 255 GGCGTC AluI 143 AGCT AG^CT 326 AGCT AvaI 30 CTCGGG C^[CT]CG[GA]G 546 CCCGAG BsaHI 40 GGCGCC G[GA]^CG[CT]C 255 GGCGTC BsoBI 30 CTCGGG C^[CT]CG[GA]G 546 CCCGAG BssHII 151 GCGCGC G^CGCGC 153 GCGCGC BstEII 97 GGTCACC G^GT[ACGT]ACC 330 GGTGACC BstF5I 117 CATCC GGATG(2/0) 570 CATCC BstNI 93 CCTGG CC^[AT]GG 555 CCAGG BstPI 97 GGTCACC G^GT[ACGT]ACC 330 GGTGACC DpnII 351 GATC ^GATC 459 GATC DraII 89 AGGCCCT [GA]G^G[ACGT]CC[CT] 183 GGGGCCT EarI 322 GAAGAG CTCTTC(1/4) 427 GAAGAG EcoO109I 89 AGGCCCT [GA]G^G[ACGT]CC[CT] 183 GGGGCCT EcoO65I 97 GGTCACC G^GT[ACGT]ACC 330 GGTGACC EcoRII 93 CCTGG ^CC[AT]GG 555 CCAGG FokI 117 CATCC GGATG(9/13) 570 CATCC HaeII 40 GGCGCC [GA]GCGC^[CT] 558 GGCGCT Hin1I 40 GGCGCC G[GA]^CG[CT]C 255 GGCGTC HphI 330 GGTGA GGTGA(8/7) 99 TCACC Ksp632I 322 GAAGAG CTCTTC(1/4) 427 GAAGAG MboI 351 GATC ^GATC 459 GATC MvaI 93 CCTGG CC^[AT]GG 555 CCAGG NdeII 351 GATC ^GATC 459 GATC Sau3AI 351 GATC ^GATC 459 GATC Tsp45I 98 GTCAC ^GT[GC]AC 331 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AccII AciI AfaI BbvI BcnI BsaJI BsmAI BsrI BssKI BstUI Cac8I CfoI Cfr13I Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MboII MnlI MseI MspI MvnI MwoI NciI NlaIV PstI RsaI Sau96I ScrFI SfcI Tru9I TseI Tsp509I