Restriction map for
pFN21AE1130
(Circular) (Six-base) MAPSORT of: pFN21AE1130.seq Check: 985 from: 1 to: 5368
pFN21AE1130 5368 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2115 2115
Size: 5368
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3178 278
Size: 53 83 186 936 1642 2468
Fragments arranged by size:
2468 1642 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2502 2711 1937
Size: 565 209 4594
Fragments arranged by size:
4594 565 209
Acc65I G'GTAC_C
Cuts at: 2689 2689
Size: 5368
AclI AA'CG_TT
Cuts at: 1563 3495 3868 1563
Size: 1932 373 3063
Fragments arranged by size:
3063 1932 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3431 4815 5083 1632
Size: 1799 1384 268 1917
Fragments arranged by size:
1917 1799 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5171
AflIII A'CryG_T
Cuts at: 1203 2217 4267 5244 1203
Size: 1014 2050 977 1327
Fragments arranged by size:
2050 1327 1014 977
AgeI A'CCGG_T
Cuts at: 4199 4199
Size: 5368
AhdI GACnn_n'nnGTC
Cuts at: 4097 4097
Size: 5368
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5368
AlwNI CAG_nnn'CTG
Cuts at: 2566 3111 4683 2566
Size: 545 1572 3251
Fragments arranged by size:
3251 1572 545
ApaLI G'TGCA_C
Cuts at: 3425 4581 3425
Size: 1156 4212
ApoI r'AATT_y
Cuts at: 1493 2363 2677 2903 3055 5164 1493
Size: 870 314 226 152 2109 1697
Fragments arranged by size:
2109 1697 870 314 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3922 160
Size: 872 2890 1606
Fragments arranged by size:
2890 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2551 2693 1870
Size: 73 92 516 142 4545
Fragments arranged by size:
4545 516 142 92 73
BamHI G'GATC_C
Cuts at: 2698 3065 2698
Size: 367 5001
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2689 4144 618
Size: 325 217 627 258 644 1455 1842
Fragments arranged by size:
1842 1455 644 627 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2687 1391
Size: 1296 4072
BbeI G_GCGC'C
Cuts at: 2049 2049
Size: 5368
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2599 928
Size: 971 700 3697
Fragments arranged by size:
3697 971 700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3559 3593 1903
Size: 34 50 34 1538 34 3678
Fragments arranged by size:
3678 1538 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2489 3260 4470 2489
Size: 771 1210 3387
Fragments arranged by size:
3387 1210 771
BclI T'GATC_A
Cuts at: 1548 2355 1548
Size: 807 4561
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3979 136
Size: 107 122 71 631 605 2307 1525
Fragments arranged by size:
2307 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5363 5363
Size: 5368
BlpI GC'TnA_GC
Cuts at: 2785 2785
Size: 5368
Bme1580I G_kGCm'C
Cuts at: 1790 3429 4585 1790
Size: 1639 1156 2573
Fragments arranged by size:
2573 1639 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5368
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2121 3099 4057
Size: 416 53 83 399 24 704 978 958
Cuts at: 4057 442
Size: 1753
Fragments arranged by size:
1753 978 958 704 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5368
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2484 2484
Size: 5368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 4028 1239
Size: 872 1917 2579
Fragments arranged by size:
2579 1917 872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3493 4358 4656 3493
Size: 865 298 4205
Fragments arranged by size:
4205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2629 4031 882
Size: 617 1130 1402 2219
Fragments arranged by size:
2219 1402 1130 617
BsaAI yAC'GTr
Cuts at: 493 1204 3159 493
Size: 711 1955 2702
Fragments arranged by size:
2702 1955 711
BsaBI GATnn'nnATC
Cuts at: 5362 5362
Size: 5368
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2261 3175 3557
Size: 53 83 186 936 513 215 914 382
Cuts at: 3557 4192 275
Size: 635 1451
Fragments arranged by size:
1451 936 914 635 513 382 215 186
83 53
BsaWI w'CCGG_w
Cuts at: 3801 4199 4473 4620 5031 3801
Size: 398 274 147 411 4138
Fragments arranged by size:
4138 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 1178
Size: 30 165 30 693 30 4420
Fragments arranged by size:
4420 693 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5368
BseYI C'CCAG_C
Cuts at: 2058 4571 5067 2058
Size: 2513 496 2359
Fragments arranged by size:
2513 2359 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4795
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2506 2818 3579 4607 1540
Size: 275 186 505 312 761 1028 2301
Fragments arranged by size:
2301 1028 761 505 312 275 186
BsiHKAI G_wGCw'C
Cuts at: 3429 3514 4585 3429
Size: 85 1071 4212
Fragments arranged by size:
4212 1071 85
BsmI GAATG_Cn'
Cuts at: 2879 2972 2879
Size: 93 5275
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2687 3429 3514 4585 1391
Size: 399 897 742 85 1071 2174
Fragments arranged by size:
2174 1071 897 742 399 85
BspEI T'CCGG_A
Cuts at: 5031 5031
Size: 5368
BspHI T'CATG_A
Cuts at: 3257 4987 3257
Size: 1730 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 2656 2723 844
Size: 1271 541 67 3489
Fragments arranged by size:
3489 1271 541 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3255 1394
Size: 429 1432 3507
Fragments arranged by size:
3507 1432 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2452 3863 4037 65
Size: 1183 1204 1411 174 1396
Fragments arranged by size:
1411 1396 1204 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2505 4012 4199 1700
Size: 110 695 1507 187 2869
Fragments arranged by size:
2869 1507 695 187 110
BsrGI T'GTAC_A
Cuts at: 96 2633 96
Size: 2537 2831
BssHII G'CGCG_C
Cuts at: 1925 2156 2158 1925
Size: 231 2 5135
Fragments arranged by size:
5135 231 2
BssSI C'ACGA_G
Cuts at: 3428 4440 3428
Size: 1012 4356
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5368
BstBI TT'CG_AA
Cuts at: 4172 4172
Size: 5368
BstEII G'GTnAC_C
Cuts at: 1184 2102 2335 1184
Size: 918 233 4217
Fragments arranged by size:
4217 918 233
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5368
BstYI r'GATC_y
Cuts at: 1972 2698 3065 3450 3467 4919 5005 5017 5363
Size: 726 367 385 17 1452 86 12 346
Cuts at: 5363 1972
Size: 1977
Fragments arranged by size:
1977 1452 726 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2342 2342
Size: 5368
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4816
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4629
Fragments arranged by size:
4629 683 56
BtsI GCAGTG_nn'
Cuts at: 2078 2884 3678 3698 2078
Size: 806 794 20 3748
Fragments arranged by size:
3748 806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5368
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5333
DraI TTT'AAA
Cuts at: 2672 2672
Size: 5368
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4375 809
Size: 507 3059 1802
Fragments arranged by size:
3059 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2815 3704 5333
Size: 54 1425 50 275 18 985 889 1629
Cuts at: 5333 8
Size: 43
Fragments arranged by size:
1629 1425 985 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2815 1537
Size: 275 1003 4090
Fragments arranged by size:
4090 1003 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2322 2427 3298 1353
Size: 969 105 871 3423
Fragments arranged by size:
3423 969 871 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2104 3955 4329 4475 387
Size: 1064 138 515 1851 374 146 1280
Fragments arranged by size:
1851 1280 1064 515 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 3431 4028 4815 5083 1239
Size: 393 479 1320 597 787 268 1524
Fragments arranged by size:
1524 1320 787 597 479 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2663 2663
Size: 5368
EcoO109I rG'GnC_Cy
Cuts at: 2095 2189 2095
Size: 94 5274
EcoRI G'AATT_C
Cuts at: 2363 2677 3055 2363
Size: 314 378 4676
Fragments arranged by size:
4676 378 314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 5368
FspI TGC'GCA
Cuts at: 3874 3874
Size: 5368
HaeII r_GCGC'y
Cuts at: 1678 2049 2567 4515 1678
Size: 371 518 1948 2531
Fragments arranged by size:
2531 1948 518 371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4009 4041 4083 4115 1373
Size: 32 135 32 2437 32 42 32 2626
Fragments arranged by size:
2626 2437 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2503 2712 2958 669
Size: 988 281 565 209 246 3079
Fragments arranged by size:
3079 988 565 281 246 209
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5368
HpaI GTT'AAC
Cuts at: 2958 2958
Size: 5368
KasI G'GCGC_C
Cuts at: 2045 2045
Size: 5368
KpnI G_GTAC'C
Cuts at: 2693 2693
Size: 5368
MfeI C'AATT_G
Cuts at: 2967 2967
Size: 5368
MluI A'CGCG_T
Cuts at: 5244 5244
Size: 5368
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 2421 4482 4666 1305
Size: 816 300 2061 184 2007
Fragments arranged by size:
2061 2007 816 300 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3546
Fragments arranged by size:
3546 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 3326 3685 3844 518
Size: 648 1419 741 359 159 2042
Fragments arranged by size:
2042 1419 741 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2149 2784 3461 4609 4854 1606
Size: 543 635 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 635 543 245
NarI GG'CG_CC
Cuts at: 2046 2046
Size: 5368
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4816
NdeI CA'TA_TG
Cuts at: 387 2386 387
Size: 1999 3369
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5368
NotI GC'GGCC_GC
Cuts at: 2815 2815
Size: 5368
NspI r_CATG'y
Cuts at: 1132 1229 2726 4271 1132
Size: 97 1497 1545 2229
Fragments arranged by size:
2229 1545 1497 97
PasI CC'CwG_GG
Cuts at: 1348 2098 1348
Size: 750 4618
PciI A'CATG_T
Cuts at: 4267 4267
Size: 5368
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5368
PfoI T'CCnGG_A
Cuts at: 2199 2199
Size: 5368
PmeI GTTT'AAAC
Cuts at: 2672 2672
Size: 5368
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 4950 4982 3437
Size: 32 1481 32 3823
Fragments arranged by size:
3823 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5368
PsiI TTA'TAA
Cuts at: 2938 2938
Size: 5368
PstI C_TGCA'G
Cuts at: 830 2073 2154 2529 2667 2720 830
Size: 1243 81 375 138 53 3478
Fragments arranged by size:
3478 1243 375 138 81 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5368
PvuII CAG'CTG
Cuts at: 2149 2149
Size: 5368
SalI G'TCGA_C
Cuts at: 1936 2501 2710 1936
Size: 565 209 4594
Fragments arranged by size:
4594 565 209
SapI GCTCTTCn'nnn_
Cuts at: 2322 2322
Size: 5368
SbfI CC_TGCA'GG
Cuts at: 2720 2720
Size: 5368
ScaI AGT'ACT
Cuts at: 2492 3616 5328 2492
Size: 1124 1712 2532
Fragments arranged by size:
2532 1712 1124
SfcI C'TryA_G
Cuts at: 826 1045 2069 2150 2525 2663 2716 3851 4532
Size: 219 1024 81 375 138 53 1135 681
Cuts at: 4532 4723 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1024 681 375 219 191 138
81 53
SfoI GGC'GCC
Cuts at: 2047 2047
Size: 5368
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5368
SmaI CCC'GGG
Cuts at: 1872 2695 1872
Size: 823 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3472 4373 4635 820
Size: 197 926 1529 901 262 1553
Fragments arranged by size:
1553 1529 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3159 493
Size: 2666 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5368
SphI G_CATG'C
Cuts at: 1132 2726 1132
Size: 1594 3774
SspI AAT'ATT
Cuts at: 5 52 3292 5
Size: 47 3240 2081
Fragments arranged by size:
3240 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5368
StyI C'CwwG_G
Cuts at: 513 1065 2082 2807 513
Size: 552 1017 725 3074
Fragments arranged by size:
3074 1017 725 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3395 3412 3565 3750 4195 325
Size: 1255 1815 17 153 185 445 1498
Fragments arranged by size:
1815 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2490 2633 3614
Size: 274 80 33 51 1443 513 143 981
Cuts at: 3614 5326 96
Size: 1712 138
Fragments arranged by size:
1712 1443 981 513 274 143 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2096 2128 5328 5360 2096
Size: 32 3200 32 2104
Fragments arranged by size:
3200 2104 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5368
XbaI T'CTAG_A
Cuts at: 2704 2704
Size: 5368
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5368
XmaI C'CCGG_G
Cuts at: 1870 2693 1870
Size: 823 4545
XmnI GAAnn'nnTTC
Cuts at: 3497 3497
Size: 5368
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3176 276
Size: 53 83 186 936 1642 2468
Fragments arranged by size:
2468 1642 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI XcmI