################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AciI AclI AcyI AflII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BciVI BclI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRV FauI FbaI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PaeR7I PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AfaI 733 GTAC GT^AC AhdI 729 GACTGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 729 GACTGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 952 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 152 CCCGAG C^[CT]CG[GA]G BbrPI 1020 CACGTG CAC^GTG BbvCI 143 CCTCAGC CCTCAGC(-5/-2) BbvI 486 GCAGC GCAGC(8/12) BcgI 967 CGAAATTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 120 CCCGG CC^[GC]GG BglII 171 AGATCT A^GATCT BsaAI 1020 CACGTG [CT]AC^GT[GA] BsmFI 616 GGGAC GGGAC(10/14) BsoBI 152 CCCGAG C^[CT]CG[GA]G BstAPI 884 GCAACTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1392 TTCGAA TT^CGAA BstEII 220 GGTAACC G^GT[ACGT]ACC BstPI 220 GGTAACC G^GT[ACGT]ACC BstYI 171 AGATCT [GA]^GATC[CT] Cfr13I 372 GGCCC G^G[ACGT]CC DraIII 241 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 546 CCATGG C^C[GA][CT]GG Eam1105I 729 GACTGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 220 GGTAACC G^GT[ACGT]ACC EcoT14I 546 CCATGG C^C[AT][AT]GG EcoT22I 663 ATGCAT ATGCA^T Fnu4HI 486 GCAGC GC^[ACGT]GC HaeIII 372 GGCC GG^CC HapII 121 CCGG C^CGG HgaI 816 GCGTC GACGC(5/10) HindIII 459 AAGCTT A^AGCTT HpaII 121 CCGG C^CGG HphI 562 TCACC GGTGA(8/7) ItaI 486 GCAGC GC^[ACGT]GC MflI 171 AGATCT [GA]^GATC[CT] MspI 121 CCGG C^CGG NciI 120 CCCGG CC^[GC]GG NcoI 546 CCATGG C^CATGG NsiI 663 ATGCAT ATGCA^T NspV 1392 TTCGAA TT^CGAA PflFI 952 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1320 GAGTC GAGTC(4/5) PmaCI 1020 CACGTG CAC^GTG PmlI 1020 CACGTG CAC^GTG PstI 484 CTGCAG CTGCA^G RsaI 733 GTAC GT^AC Sau96I 372 GGCCC G^G[ACGT]CC SexAI 60 ACCTGGT A^CC[AT]GGT SfuI 1392 TTCGAA TT^CGAA StyI 546 CCATGG C^C[AT][AT]GG TseI 486 GCAGC G^C[AT]GC Tth111I 952 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 171 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 409 CGCG CG^CG 815 CGCG AflIII 1019 ACACGT A^C[GA][CT]GT 1021 ACGTGT BbsI 958 GTCTTC GAAGAC(2/6) 1210 GTCTTC BfaI 202 CTAG C^TAG 1571 CTAG BpuAI 958 GTCTTC GAAGAC(2/6) 1210 GTCTTC BsaI 401 GAGACC GGTCTC(1/5) 64 GGTCTC BsiYI 69 CCCTGGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1671 CCATACTAAGG BslI 69 CCCTGGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1671 CCATACTAAGG BsmI 1389 GCATTC GAATGC(1/-1) 1473 GCATTC BstUI 409 CGCG CG^CG 815 CGCG DpnII 172 GATC ^GATC 775 GATC EcoRI 1163 GAATTC G^AATTC 1247 GAATTC HincII 541 GTCAAC GT[CT]^[GA]AC 1056 GTCAAC HindII 541 GTCAAC GT[CT]^[GA]AC 1056 GTCAAC MaeI 202 CTAG C^TAG 1571 CTAG MaeII 735 ACGT A^CGT 1021 ACGT MboI 172 GATC ^GATC 775 GATC MvnI 409 CGCG CG^CG 815 CGCG NdeII 172 GATC ^GATC 775 GATC Sau3AI 172 GATC ^GATC 775 GATC SfcI 52 CTACAG C^T[GA][CT]AG 484 CTGCAG TaqI 966 TCGA T^CGA 1393 TCGA TthHB8I 966 TCGA T^CGA 1393 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AcsI AluI ApoI BpmI BsaJI BsmAI BsrDI BsrI BssKI BstF5I BstNI CfoI DdeI EarI EcoRII FokI HhaI HinP1I HinfI Ksp632I MaeIII MboII MnlI MseI MslI MvaI MwoI NlaIII NlaIV ScrFI TfiI Tru9I Tsp45I Tsp509I TspRI