(Circular) (Six-base) MAPSORT of: pFN21AE1162.seq Check: 2436 from: 1 to: 6406 pFN21AE1162 6406 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3749 1937 Size: 1812 4594 Acc65I G'GTAC_C Cuts at: 3727 3727 Size: 6406 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2281 2526 2950 3202 3454 3643 4469 5853 Size: 649 245 424 252 252 189 826 1384 Cuts at: 5853 6121 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 826 649 424 268 252 252 245 189 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 3024 3026 5305 6282 1203 Size: 1821 2 2279 977 1327 Fragments arranged by size: 2279 1821 1327 977 2 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 2739 5135 2739 Size: 2396 4010 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6406 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6406 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2055 2087 2055 Size: 32 6374 AlwNI CAG_nnn'CTG Cuts at: 4149 5721 4149 Size: 1572 4834 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 2514 2974 3168 3252 3715 3941 4093 6202 Size: 1021 460 194 84 463 226 152 2109 Cuts at: 6202 1493 Size: 1697 Fragments arranged by size: 2109 1697 1021 463 460 226 194 152 84 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 2157 3731 1870 Size: 73 214 1574 4545 Fragments arranged by size: 4545 1574 214 73 BamHI G'GATC_C Cuts at: 3736 4103 3736 Size: 367 6039 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3727 5182 618 Size: 325 217 627 1940 1455 1842 Fragments arranged by size: 1940 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3725 1391 Size: 2334 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2956 3208 928 Size: 971 1057 252 4126 Fragments arranged by size: 4126 1057 971 252 BbvCI CC'TCA_GC Cuts at: 2149 2149 Size: 6406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2961 2995 4597 4631 1903 Size: 34 50 34 940 34 1602 34 3678 Fragments arranged by size: 3678 1602 940 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4298 5508 4298 Size: 1210 5196 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BfrBI ATG'CAT Cuts at: 2670 2670 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5017 136 Size: 107 122 71 631 605 3345 1525 Fragments arranged by size: 3345 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2176 6401 2176 Size: 4225 2181 BlpI GC'TnA_GC Cuts at: 3823 3823 Size: 6406 Bme1580I G_kGCm'C Cuts at: 1790 4467 5623 1790 Size: 2677 1156 2573 Fragments arranged by size: 2677 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3186 4137 5095 Size: 416 53 83 399 24 1769 951 958 Cuts at: 5095 442 Size: 1753 Fragments arranged by size: 1769 1753 958 951 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2778 2862 2946 3282 3366 3450 3534 Size: 863 676 84 84 336 84 84 84 Cuts at: 3534 5066 1239 Size: 1532 2579 Fragments arranged by size: 2579 1532 863 676 336 84 84 84 84 84 Bpu10I CC'TnA_GC Cuts at: 2149 2149 Size: 6406 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4531 5396 5694 4531 Size: 865 298 5243 Fragments arranged by size: 5243 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2075 2400 5069 882 Size: 617 576 325 2669 2219 Fragments arranged by size: 2669 2219 617 576 325 BsaAI yAC'GTr Cuts at: 493 1204 3027 4197 493 Size: 711 1823 1170 2702 Fragments arranged by size: 2702 1823 1170 711 BsaBI GATnn'nnATC Cuts at: 6400 6400 Size: 6406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4213 4595 5230 275 Size: 53 83 186 936 2680 382 635 1451 Fragments arranged by size: 2680 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4839 5237 5511 5658 6069 4839 Size: 398 274 147 411 5176 Fragments arranged by size: 5176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2055 2085 1178 Size: 30 165 30 652 30 5499 Fragments arranged by size: 5499 652 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6406 BseYI C'CCAG_C Cuts at: 2165 5609 6105 2165 Size: 3444 496 2466 Fragments arranged by size: 3444 2466 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5833 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3856 4617 5645 1540 Size: 275 186 1855 761 1028 2301 Fragments arranged by size: 2301 1855 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4467 4552 5623 4467 Size: 85 1071 5250 Fragments arranged by size: 5250 1071 85 BsmI GAATG_Cn' Cuts at: 3394 3478 3917 4010 3394 Size: 84 439 93 5790 Fragments arranged by size: 5790 439 93 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3725 4467 4552 5623 1391 Size: 399 1935 742 85 1071 2174 Fragments arranged by size: 2174 1935 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 4295 6025 4295 Size: 1730 4676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3761 844 Size: 2917 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4293 1394 Size: 429 2470 3507 Fragments arranged by size: 3507 2470 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2095 3121 3289 4901 5075 65 Size: 1183 847 1026 168 1612 174 1396 Fragments arranged by size: 1612 1396 1183 1026 847 174 168 BsrFI r'CCGG_y Cuts at: 1700 1810 5050 5237 1700 Size: 110 3240 187 2869 Fragments arranged by size: 3240 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6406 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 4466 5478 4466 Size: 1012 5394 BstAPI GCAn_nnn'nTGC Cuts at: 794 2895 794 Size: 2101 4305 BstBI TT'CG_AA Cuts at: 3398 5210 3398 Size: 1812 4594 BstEII G'GTnAC_C Cuts at: 1184 2225 1184 Size: 1041 5365 BstYI r'GATC_y Cuts at: 1972 2176 3736 4103 4488 4505 5957 6043 6055 Size: 204 1560 367 385 17 1452 86 12 Cuts at: 6055 6401 1972 Size: 346 1977 Fragments arranged by size: 1977 1560 1452 385 367 346 204 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2551 513 Size: 552 1486 4368 Fragments arranged by size: 4368 1486 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5667 Fragments arranged by size: 5667 683 56 BtsI GCAGTG_nn' Cuts at: 2107 3922 4716 4736 2107 Size: 1815 794 20 3777 Fragments arranged by size: 3777 1815 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3103 3138 3271 3306 529 Size: 35 2539 35 133 35 3629 Fragments arranged by size: 3629 2539 133 35 35 35 DraI TTT'AAA Cuts at: 3710 3710 Size: 6406 DraIII CAC_nnn'GTG Cuts at: 2251 2251 Size: 6406 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3853 4742 6371 Size: 54 1425 50 275 18 2023 889 1629 Cuts at: 6371 8 Size: 43 Fragments arranged by size: 2023 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3853 1537 Size: 275 2041 4090 Fragments arranged by size: 4090 2041 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2280 2416 3662 4336 1353 Size: 927 136 1246 674 3423 Fragments arranged by size: 3423 1246 927 674 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4993 5367 5513 387 Size: 1064 138 3404 374 146 1280 Fragments arranged by size: 3404 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2281 2526 2778 2862 2946 2950 Size: 393 470 179 245 252 84 84 4 Cuts at: 2950 3202 3282 3366 3450 3454 3534 3643 4469 Size: 252 80 84 84 4 80 109 826 Cuts at: 4469 5066 5853 6121 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 826 787 597 470 393 268 252 252 245 179 109 84 84 84 84 80 80 4 4 EcoRI G'AATT_C Cuts at: 3168 3252 3715 4093 3168 Size: 84 463 378 5481 Fragments arranged by size: 5481 463 378 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2408 2408 Size: 6406 FspI TGC'GCA Cuts at: 4912 4912 Size: 6406 HaeII r_GCGC'y Cuts at: 1678 5553 1678 Size: 3875 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2161 2193 2725 2757 5047 Size: 32 135 32 589 32 532 32 2290 Cuts at: 5047 5079 5121 5153 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2290 589 532 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2548 3063 3750 3996 669 Size: 988 281 610 515 687 246 3079 Fragments arranged by size: 3079 988 687 610 515 281 246 HindIII A'AGCT_T Cuts at: 748 2464 748 Size: 1716 4690 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KpnI G_GTAC'C Cuts at: 3731 3731 Size: 6406 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2029 5520 5704 1305 Size: 724 3491 184 2007 Fragments arranged by size: 3491 2007 724 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4584 Fragments arranged by size: 4584 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2481 2857 3193 4364 4723 4882 518 Size: 648 1315 376 336 1171 359 159 2042 Fragments arranged by size: 2042 1315 1171 648 376 359 336 159 MspA1I CmG'CkG Cuts at: 1606 3822 4499 5647 5892 1606 Size: 2216 677 1148 245 2120 Fragments arranged by size: 2216 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2551 513 Size: 552 1486 4368 Fragments arranged by size: 4368 1486 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6406 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NsiI A_TGCA'T Cuts at: 2672 2672 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 3764 5309 1132 Size: 97 2535 1545 2229 Fragments arranged by size: 2535 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2075 1348 Size: 727 5679 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6406 PfoI T'CCnGG_A Cuts at: 2124 2124 Size: 6406 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PmlI CAC'GTG Cuts at: 3027 3027 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2055 2087 4475 4507 5988 6020 2055 Size: 32 2388 32 1481 32 2441 Fragments arranged by size: 2441 2388 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 3696 3976 3696 Size: 280 6126 PstI C_TGCA'G Cuts at: 830 2493 3758 830 Size: 1663 1265 3478 Fragments arranged by size: 3478 1663 1265 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 4654 6366 4654 Size: 1712 4694 SexAI A'CCwGG_T Cuts at: 2065 2065 Size: 6406 SfcI C'TryA_G Cuts at: 826 1045 2057 2489 3754 4889 5570 5761 826 Size: 219 1012 432 1265 1135 681 191 1471 Fragments arranged by size: 1471 1265 1135 1012 681 432 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 3733 1872 Size: 1861 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4510 5411 5673 820 Size: 197 926 2567 901 262 1553 Fragments arranged by size: 2567 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6406 StyI C'CwwG_G Cuts at: 513 1065 2551 3845 513 Size: 552 1486 1294 3074 Fragments arranged by size: 3074 1486 1294 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4433 4450 4603 4788 5233 325 Size: 1255 2853 17 153 185 445 1498 Fragments arranged by size: 2853 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4652 6364 96 Size: 274 80 33 51 1443 2675 1712 138 Fragments arranged by size: 2675 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6366 6398 6366 Size: 32 6374 Tth111I GACn'n_nGTC Cuts at: 1316 2960 1316 Size: 1644 4762 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 3731 1870 Size: 1861 4545 XmnI GAAnn'nnTTC Cuts at: 4535 4535 Size: 6406 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI