(Circular) (Six-base) MAPSORT of: pFN21AB4905.seq Check: 9922 from: 1 to: 5761 pFN21AB4905 5761 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 2517 3082 2517 Size: 565 5196 AclI AA'CG_TT Cuts at: 1563 3060 3888 4261 1563 Size: 1497 828 373 3063 Fragments arranged by size: 3063 1497 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3824 5208 5476 1632 Size: 2192 1384 268 1917 Fragments arranged by size: 2192 1917 1384 268 AfeI AGC'GCT Cuts at: 2153 2153 Size: 5761 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 2859 4660 5637 1203 Size: 1656 1801 977 1327 Fragments arranged by size: 1801 1656 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 2477 1166 Size: 1311 4450 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2495 1184 Size: 1311 4450 AlwNI CAG_nnn'CTG Cuts at: 3504 5076 3504 Size: 1572 4189 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 3070 3296 3448 5557 1493 Size: 1577 226 152 2109 1697 Fragments arranged by size: 2109 1697 1577 226 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 3086 1870 Size: 73 1143 4545 Fragments arranged by size: 4545 1143 73 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2246 2517 2532 3082 4537 Size: 325 217 627 459 271 15 550 1455 Cuts at: 4537 618 Size: 1842 Fragments arranged by size: 1842 1455 627 550 459 325 271 217 15 BanII G_rGCy'C Cuts at: 1391 2778 2933 3006 3080 1391 Size: 1387 155 73 74 4072 Fragments arranged by size: 4072 1387 155 74 73 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4790 BbvCI CC'TCA_GC Cuts at: 2733 2733 Size: 5761 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2141 3653 4863 2141 Size: 1512 1210 3039 Fragments arranged by size: 3039 1512 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2198 2661 2759 Size: 107 122 71 631 605 526 463 98 Cuts at: 2759 2958 4372 136 Size: 199 1414 1525 Fragments arranged by size: 1525 1414 631 605 526 463 199 122 107 98 71 BglII A'GATC_T Cuts at: 5756 5756 Size: 5761 BlpI GC'TnA_GC Cuts at: 2792 3178 2792 Size: 386 5375 Bme1580I G_kGCm'C Cuts at: 1790 2484 2535 2927 3822 4978 1790 Size: 694 51 392 895 1156 2573 Fragments arranged by size: 2573 1156 895 694 392 51 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3492 4450 442 Size: 416 53 83 399 24 2075 958 1753 Fragments arranged by size: 2075 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2071 2071 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2272 2939 3016 3020 4421 1239 Size: 1033 667 77 4 1401 2579 Fragments arranged by size: 2579 1401 1033 667 77 4 Bpu10I CC'TnA_GC Cuts at: 2733 2831 2733 Size: 98 5663 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3026 3886 4751 5049 3026 Size: 860 865 298 3738 Fragments arranged by size: 3738 865 860 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2719 3552 493 Size: 711 1515 833 2702 Fragments arranged by size: 2702 1515 833 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2620 2939 3568 3950 Size: 53 83 186 936 1087 319 629 382 Cuts at: 3950 4585 275 Size: 635 1451 Fragments arranged by size: 1451 1087 936 635 629 382 319 186 83 53 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5536 Fragments arranged by size: 5536 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5761 BseYI C'CCAG_C Cuts at: 2522 4964 5460 2522 Size: 2442 496 2823 Fragments arranged by size: 2823 2442 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5188 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2665 3211 3972 5000 1540 Size: 275 186 664 546 761 1028 2301 Fragments arranged by size: 2301 1028 761 664 546 275 186 BsiHKAI G_wGCw'C Cuts at: 2679 2814 3822 3907 4978 2679 Size: 135 1008 85 1071 3462 Fragments arranged by size: 3462 1071 1008 135 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2484 2535 2679 2778 2814 2927 2933 Size: 399 694 51 144 99 36 113 6 Cuts at: 2933 3006 3080 3822 3907 4978 1391 Size: 73 74 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 694 399 144 113 99 85 74 73 51 36 6 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3116 844 Size: 2272 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2349 2985 2996 3648 1394 Size: 429 526 636 11 652 3507 Fragments arranged by size: 3507 652 636 526 429 11 BsrDI GCAATG_nn' Cuts at: 65 1248 4256 4430 65 Size: 1183 3008 174 1396 Fragments arranged by size: 3008 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2660 2753 2867 4405 4592 1700 Size: 110 850 93 114 1538 187 2869 Fragments arranged by size: 2869 1538 850 187 114 110 93 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 2745 1925 Size: 820 4941 BssSI C'ACGA_G Cuts at: 2777 3821 4833 2777 Size: 1044 1012 3705 Fragments arranged by size: 3705 1044 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5761 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstXI CCAn_nnnn'nTGG Cuts at: 2401 2917 2401 Size: 516 5245 BstYI r'GATC_y Cuts at: 1972 3091 3458 3843 3860 5312 5398 5410 5756 Size: 1119 367 385 17 1452 86 12 346 Cuts at: 5756 1972 Size: 1977 Fragments arranged by size: 1977 1452 1119 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2513 2953 513 Size: 552 1448 440 3321 Fragments arranged by size: 3321 1448 552 440 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2414 2800 487 Size: 683 56 1188 386 3448 Fragments arranged by size: 3448 1188 683 386 56 BtsI GCAGTG_nn' Cuts at: 2160 2211 3024 3277 4071 4091 2160 Size: 51 813 253 794 20 3830 Fragments arranged by size: 3830 813 794 253 51 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DraIII CAC_nnn'GTG Cuts at: 2042 2722 2042 Size: 680 5081 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2199 2331 Size: 54 1425 50 275 18 360 9 132 Cuts at: 2331 2448 2598 2662 2751 2755 3208 4097 5726 Size: 117 150 64 89 4 453 889 1629 Cuts at: 5726 8 Size: 43 Fragments arranged by size: 1629 1425 889 453 360 275 150 132 117 89 64 54 50 43 18 9 4 EagI C'GGCC_G Cuts at: 1537 1812 2662 3208 1537 Size: 275 850 546 4090 Fragments arranged by size: 4090 850 546 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3691 1353 Size: 2338 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2633 4348 4722 4868 387 Size: 1064 138 1044 1715 374 146 1280 Fragments arranged by size: 1715 1280 1064 1044 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2272 2939 3016 3020 3824 4421 5208 Size: 393 640 667 77 4 804 597 787 Cuts at: 5208 5476 1239 Size: 268 1524 Fragments arranged by size: 1524 804 787 667 640 597 393 268 77 4 EcoNI CCTnn'n_nnAGG Cuts at: 2732 2732 Size: 5761 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FseI GG_CCGG'CC Cuts at: 2757 2757 Size: 5761 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 2090 2155 2641 4908 1678 Size: 412 65 486 2267 2531 Fragments arranged by size: 2531 2267 486 412 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4402 4434 4476 4508 1373 Size: 32 135 32 2830 32 42 32 2626 Fragments arranged by size: 2830 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3105 3351 669 Size: 988 281 1167 246 3079 Fragments arranged by size: 3079 1167 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 2521 3086 2521 Size: 565 5196 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 2859 5637 2859 Size: 2778 2983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4875 5059 1305 Size: 3570 184 2007 Fragments arranged by size: 3570 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2450 2600 10 Size: 54 1425 343 618 150 3171 Fragments arranged by size: 3171 1425 618 343 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 2333 2429 2477 3719 4078 4237 518 Size: 648 1167 96 48 1242 359 159 2042 Fragments arranged by size: 2042 1242 1167 648 359 159 96 48 MspA1I CmG'CkG Cuts at: 1606 2515 2955 3177 3854 5002 5247 1606 Size: 909 440 222 677 1148 245 2120 Fragments arranged by size: 2120 1148 909 677 440 245 222 NaeI GCC'GGC Cuts at: 2755 2869 2755 Size: 114 5647 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5209 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NgoMIV G'CCGG_C Cuts at: 2753 2867 2753 Size: 114 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 2308 3119 4664 1132 Size: 97 1079 811 1545 2229 Fragments arranged by size: 2229 1545 1079 811 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5761 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PmlI CAC'GTG Cuts at: 2719 2719 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PstI C_TGCA'G Cuts at: 830 2026 3113 830 Size: 1196 1087 3478 Fragments arranged by size: 3478 1196 1087 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 SacII CC_GC'GG Cuts at: 2516 2956 2516 Size: 440 5321 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SfcI C'TryA_G Cuts at: 826 1045 2022 2627 3109 4244 4925 5116 826 Size: 219 977 605 482 1135 681 191 1471 Fragments arranged by size: 1471 1135 977 681 605 482 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2198 2759 2198 Size: 561 5200 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 2867 1700 Size: 1167 4594 SmaI CCC'GGG Cuts at: 1872 3088 1872 Size: 1216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3041 3865 4766 5028 820 Size: 197 926 1098 824 901 262 1553 Fragments arranged by size: 1553 1098 926 901 824 262 197 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2242 2394 3200 513 Size: 552 1177 152 806 3074 Fragments arranged by size: 3074 1177 806 552 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2419 3788 3805 3958 4143 4588 325 Size: 1255 839 1369 17 153 185 445 1498 Fragments arranged by size: 1498 1369 1255 839 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4007 5719 96 Size: 274 80 33 51 1443 2030 1712 138 Fragments arranged by size: 2030 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2811 2843 5721 5753 2811 Size: 32 2878 32 2819 Fragments arranged by size: 2878 2819 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 2341 3097 2341 Size: 756 5005 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 3086 1870 Size: 1216 4545 XmnI GAAnn'nnTTC Cuts at: 3890 3890 Size: 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfoI SrfI SwaI XcmI