Restriction map for
pFN21AE1127
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III EcoO65I EcoRI EcoRII
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI MvaI
MvnI NaeI NarI NdeI NgoMIV
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 219 GTAC GT^AC
AocI 319 CCTAAGG CC^T[ACGT]AGG
AvaI 297 CCCGGG C^[CT]CG[GA]G
AvaII 203 GGACC G^G[AT]CC
BamHI 281 GGATCC G^GATCC
BanI 324 GGTGCC G^G[CT][GA]CC
BbsI 150 GAAGAC GAAGAC(2/6)
BfaI 103 CTAG C^TAG
BpuAI 150 GAAGAC GAAGAC(2/6)
BseRI 4 GAGGAG GAGGAG(10/8)
BsgI 262 CTGCAC GTGCAG(16/14)
BsiEI 11 CGGCCG CG[GA][CT]^CG
BsoBI 297 CCCGGG C^[CT]CG[GA]G
BstF5I 119 GGATG GGATG(2/0)
BstYI 281 GGATCC [GA]^GATC[CT]
Bsu36I 319 CCTAAGG CC^T[ACGT]AGG
DdeI 320 CTAAG C^T[ACGT]AG
DpnII 282 GATC ^GATC
DraII 202 GGGACCC [GA]G^G[ACGT]CC[CT]
EagI 11 CGGCCG C^GGCCG
EclXI 11 CGGCCG C^GGCCG
Eco52I 11 CGGCCG C^GGCCG
Eco81I 319 CCTAAGG CC^T[ACGT]AGG
EcoNI 207 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 202 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 59 CCATGG C^C[AT][AT]GG
FokI 119 GGATG GGATG(9/13)
HphI 90 TCACC GGTGA(8/7)
MaeI 103 CTAG C^TAG
MaeII 248 ACGT A^CGT
MboI 282 GATC ^GATC
MflI 281 GGATCC [GA]^GATC[CT]
MspA1I 175 CAGCTG C[AC]G^C[GT]G
NcoI 59 CCATGG C^CATGG
NdeII 282 GATC ^GATC
NlaIII 60 CATG CATG^
NotI 10 GCGGCCGC GC^GGCCGC
PleI 313 GAGTC GAGTC(4/5)
PpuMI 202 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 175 CAGCTG CAG^CTG
RsaI 219 GTAC GT^AC
Sau3AI 282 GATC ^GATC
SmaI 297 CCCGGG CCC^GGG
StyI 59 CCATGG C^C[AT][AT]GG
XhoII 281 GGATCC [GA]^GATC[CT]
XmaCI 297 CCCGGG C^CCGGG
XmaI 297 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 282 GATCC GGATC(4/5)
281 GGATC
BpmI 178 CTGGAG CTGGAG(16/14)
241 CTGGAG
BsmFI 202 GGGAC GGGAC(10/14)
315 GTCCC
BspMI 92 ACCTGC ACCTGC(4/8)
257 ACCTGC
Cac8I 81 GCAGGC GC[ACGT]^[ACGT]GC
135 GCTGGC
Cfr13I 191 GGGCC G^G[ACGT]CC
203 GGACC
EaeI 11 CGGCCG [CT]^GGCC[GA]
122 TGGCCG
HapII 116 CCGG C^CGG
298 CCGG
HpaII 116 CCGG C^CGG
298 CCGG
MspI 116 CCGG C^CGG
298 CCGG
PstI 211 CTGCAG CTGCA^G
229 CTGCAG
Sau96I 191 GGGCC G^G[ACGT]CC
203 GGACC
SfcI 211 CTGCAG C^T[GA][CT]AG
229 CTGCAG
TfiI 51 GATTC G^A[AT]TC
76 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BssKI DsaI Fnu4HI
HaeIII HinfI ItaI MboII MnlI
MwoI NciI NlaIV ScrFI TseI