(Linear) (Six-base) MAPSORT of: KIEE1127.seq Check: 2910 from: 1 to: 354 KIEE1127.orf With 182 enzymes: SgfI * July 15, 2010 18:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 101 354 Size: 101 253 AvaI C'yCGr_G Cuts at: 0 297 354 Size: 297 57 BamHI G'GATC_C Cuts at: 0 281 354 Size: 281 73 BanI G'GyrC_C Cuts at: 0 324 354 Size: 324 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 157 354 Size: 157 197 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 262 354 Size: 199 63 92 Fragments arranged by size: 199 92 63 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 172 202 354 Size: 172 30 152 Fragments arranged by size: 172 152 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 354 Size: 19 335 BseYI C'CCAG_C Cuts at: 0 198 354 Size: 198 156 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 354 Size: 247 107 BsiEI CG_ry'CG Cuts at: 0 14 354 Size: 14 340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 101 266 354 Size: 101 165 88 Fragments arranged by size: 165 101 88 BstYI r'GATC_y Cuts at: 0 281 354 Size: 281 73 Bsu36I CC'TnA_GG Cuts at: 0 320 354 Size: 320 34 BtgI C'CryG_G Cuts at: 0 31 59 158 354 Size: 31 28 99 196 Fragments arranged by size: 196 99 31 28 EaeI y'GGCC_r Cuts at: 0 11 122 354 Size: 11 111 232 Fragments arranged by size: 232 111 11 EagI C'GGCC_G Cuts at: 0 11 354 Size: 11 343 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 262 354 Size: 199 63 92 Fragments arranged by size: 199 92 63 EcoNI CCTnn'n_nnAGG Cuts at: 0 211 354 Size: 211 143 EcoO109I rG'GnC_Cy Cuts at: 0 203 354 Size: 203 151 MspA1I CmG'CkG Cuts at: 0 177 354 Size: 177 177 NcoI C'CATG_G Cuts at: 0 59 354 Size: 59 295 NotI GC'GGCC_GC Cuts at: 0 11 354 Size: 11 343 PpuMI rG'GwC_Cy Cuts at: 0 203 354 Size: 203 151 PstI C_TGCA'G Cuts at: 0 215 233 354 Size: 215 18 121 Fragments arranged by size: 215 121 18 PvuII CAG'CTG Cuts at: 0 177 354 Size: 177 177 SanDI GG'GwC_CC Cuts at: 0 203 354 Size: 203 151 SfcI C'TryA_G Cuts at: 0 211 229 354 Size: 211 18 125 Fragments arranged by size: 211 125 18 SmaI CCC'GGG Cuts at: 0 299 354 Size: 299 55 StyI C'CwwG_G Cuts at: 0 59 354 Size: 59 295 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 82 114 172 204 354 Size: 82 32 58 32 150 Fragments arranged by size: 150 82 58 32 32 XmaI C'CCGG_G Cuts at: 0 297 354 Size: 297 57 Enzymes that do cut: AarI AvaI BamHI BanI BbsI BpmI BsaXI BseRI BseYI BsgI BsiEI BspMI BstYI Bsu36I BtgI EaeI EagI Eco57MI EcoNI EcoO109I MspA1I NcoI NotI PpuMI PstI PvuII SanDI SfcI SmaI StyI TstI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NdeI NgoMIV NheI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmnI ZraI