Restriction map for
pFN21AB8287
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I Van91I XbaI XhoI
XhoII
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 897 GTCTAC GT^[AC][GT]AC
AcyI 577 GACGCC G[GA]^CG[CT]C
AgeI 893 ACCGGT A^CCGGT
AlwNI 202 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 65 CCCGGG C^[CT]CG[GA]G
BsaAI 1149 CACGTA [CT]AC^GT[GA]
BsaHI 577 GACGCC G[GA]^CG[CT]C
BseRI 70 GAGGAG GAGGAG(10/8)
BsmBI 7 GAGACG CGTCTC(1/5)
BsoBI 65 CCCGGG C^[CT]CG[GA]G
BsrBI 307 GAGCGG CCGCTC(-3/-3)
BsrDI 698 GCAATG GCAATG(2/0)
BsrFI 893 ACCGGT [GA]^CCGG[CT]
BstEII 33 GGTGACC G^GT[ACGT]ACC
BstPI 33 GGTGACC G^GT[ACGT]ACC
CfoI 22 GCGC GCG^C
Cfr10I 893 ACCGGT [GA]^CCGG[CT]
DraIII 1119 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 219 GAAGAG CTCTTC(1/4)
EcoNI 435 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 33 GGTGACC G^GT[ACGT]ACC
EcoT22I 701 ATGCAT ATGCA^T
HhaI 22 GCGC GCG^C
Hin1I 577 GACGCC G[GA]^CG[CT]C
HinP1I 22 GCGC G^CGC
Ksp632I 219 GAAGAG CTCTTC(1/4)
MaeII 1150 ACGT A^CGT
MseI 635 TTAA T^TAA
MwoI 238 GCTACCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 701 ATGCAT ATGCA^T
PinAI 893 ACCGGT A^CCGGT
PstI 1243 CTGCAG CTGCA^G
PvuII 82 CAGCTG CAG^CTG
SacI 726 GAGCTC GAGCT^C
SfcI 1243 CTGCAG C^T[GA][CT]AG
SmaI 65 CCCGGG CCC^GGG
Tru9I 635 TTAA T^TAA
Tsp509I 990 AATT ^AATT
XcmI 167 CCAAACCAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 65 CCCGGG C^CCGGG
XmaI 65 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 795 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1215 GAAGGCCTTC
BanII 726 GAGCTC G[GA]GC[CT]^C
980 GAGCCC
BsaI 127 GAGACC GGTCTC(1/5)
445 GAGACC
BsgI 502 CTGCAC GTGCAG(16/14)
245 GTGCAG
BsmFI 487 GGGAC GGGAC(10/14)
595 GGGAC
BsmI 694 GAATGC GAATGC(1/-1)
1114 GAATGC
Cac8I 309 GCGGGC GC[ACGT]^[ACGT]GC
614 GCAAGC
DdeI 305 CTGAG C^T[ACGT]AG
1065 CTCAG
DraII 100 AGGACCC [GA]G^G[ACGT]CC[CT]
919 AGGACCC
EcoO109I 100 AGGACCC [GA]G^G[ACGT]CC[CT]
919 AGGACCC
FauI 291 CCCGC CCCGC(4/6)
309 GCGGG
HgaI 16 GACGC GACGC(5/10)
577 GACGC
MboII 219 GAAGA GAAGA(8/7)
343 TCTTC
MspA1I 82 CAGCTG C[AC]G^C[GT]G
1084 CAGCGG
PleI 333 GACTC GAGTC(4/5)
556 GACTC
PpuMI 100 AGGACCC [GA]G^G[AT]CC[CT]
919 AGGACCC
SmlI 351 CTCAAG C^T[CT][GA]AG
784 CTTGAG
XmnI 795 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1215 GAAGGCCTTC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cfr13I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MnlI
MslI MspI MvaI NciI NlaIII
NlaIV NspI RsaI Sau96I ScrFI
SfaNI StuI TaqI TfiI TseI
Tsp45I TspRI TthHB8I