(Circular) (Six-base) MAPSORT of: pFN21AB8287.seq Check: 3094 from: 1 to: 5971 pFN21AB8287 5971 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2199 2243 2199 Size: 44 5927 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2903 3314 1937 Size: 966 411 4594 Fragments arranged by size: 4594 966 411 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3043 4034 5418 5686 1632 Size: 1411 991 1384 268 1917 Fragments arranged by size: 1917 1411 1384 991 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 2898 4802 2898 Size: 1904 4067 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 2212 3714 5286 2212 Size: 1502 1572 2897 Fragments arranged by size: 2897 1572 1502 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 3280 3506 3658 5767 1493 Size: 1787 226 152 2109 1697 Fragments arranged by size: 2109 1787 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 2070 3296 1870 Size: 73 127 1226 4545 Fragments arranged by size: 4545 1226 127 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3190 3223 3190 Size: 33 5938 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3292 4747 618 Size: 325 217 627 1505 1455 1842 Fragments arranged by size: 1842 1505 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2735 2989 3290 1391 Size: 1344 254 301 4072 Fragments arranged by size: 4072 1344 301 254 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5000 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BfrBI ATG'CAT Cuts at: 2708 2708 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 4032 5188 1790 Size: 2242 1156 2573 Fragments arranged by size: 2573 2242 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3111 3121 3195 Size: 416 53 83 399 24 1694 10 74 Cuts at: 3195 3702 4660 442 Size: 507 958 1753 Fragments arranged by size: 1753 1694 958 507 416 399 83 74 53 24 10 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2754 2754 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2497 2871 2955 3039 4631 1239 Size: 911 347 374 84 84 1592 2579 Fragments arranged by size: 2579 1592 911 374 347 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2341 2810 4096 4961 5259 2341 Size: 469 1286 865 298 3053 Fragments arranged by size: 3053 1286 865 469 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2126 2444 4634 882 Size: 617 627 318 2190 2219 Fragments arranged by size: 2219 2190 627 617 318 BsaAI yAC'GTr Cuts at: 493 1204 3156 3762 493 Size: 711 1952 606 2702 Fragments arranged by size: 2702 1952 711 606 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2583 3778 4160 4795 Size: 53 83 186 936 1050 1195 382 635 Cuts at: 4795 275 Size: 1451 Fragments arranged by size: 1451 1195 1050 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2428 2898 3147 4404 4802 5076 5223 5634 2428 Size: 470 249 1257 398 274 147 411 2765 Fragments arranged by size: 2765 1257 470 411 398 274 249 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5746 Fragments arranged by size: 5746 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 1186 Size: 904 5067 BseYI C'CCAG_C Cuts at: 5174 5670 5174 Size: 496 5475 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2271 2492 1163 Size: 573 535 221 4642 Fragments arranged by size: 4642 573 535 221 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2266 2735 3155 4032 4117 5188 2266 Size: 469 420 877 85 1071 3049 Fragments arranged by size: 3049 1071 877 469 420 85 BsmI GAATG_Cn' Cuts at: 2705 3125 3482 3575 2705 Size: 420 357 93 5101 Fragments arranged by size: 5101 420 357 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2006 2006 Size: 5971 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2266 2735 2989 3155 3290 4032 4117 Size: 399 476 469 254 166 135 742 85 Cuts at: 4117 5188 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 476 469 399 254 166 135 85 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2199 2243 2805 3225 3326 844 Size: 1355 44 562 420 101 3489 Fragments arranged by size: 3489 1355 562 420 101 44 BsrBI CCG'CTC Cuts at: 1394 1823 2314 3858 1394 Size: 429 491 1544 3507 Fragments arranged by size: 3507 1544 491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2710 4466 4640 65 Size: 1183 1462 1756 174 1396 Fragments arranged by size: 1756 1462 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2898 4615 4802 1700 Size: 110 1088 1717 187 2869 Fragments arranged by size: 2869 1717 1088 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5971 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 2038 1184 Size: 854 5117 BstYI r'GATC_y Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966 Size: 1329 367 385 17 1452 86 12 346 Cuts at: 5966 1972 Size: 1977 Fragments arranged by size: 1977 1452 1329 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4480 Fragments arranged by size: 4480 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 2630 3487 4281 4301 2630 Size: 857 794 20 4300 Fragments arranged by size: 4300 857 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2608 2643 2692 2727 529 Size: 35 2044 35 49 35 3773 Fragments arranged by size: 3773 2044 49 35 35 35 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DraIII CAC_nnn'GTG Cuts at: 3129 3129 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936 Size: 54 1425 50 275 18 1588 889 1629 Cuts at: 5936 8 Size: 43 Fragments arranged by size: 1629 1588 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2219 3901 1353 Size: 866 1682 3423 Fragments arranged by size: 3423 1682 866 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4558 4932 5078 387 Size: 1064 138 435 2534 374 146 1280 Fragments arranged by size: 2534 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2733 2733 Size: 5971 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2497 2871 2955 3039 3043 4034 Size: 393 518 347 374 84 84 4 991 Cuts at: 4034 4631 5418 5686 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 991 787 597 518 393 374 347 268 84 84 4 EcoNI CCTnn'n_nnAGG Cuts at: 2444 2444 Size: 5971 EcoO109I rG'GnC_Cy Cuts at: 2106 2925 2106 Size: 819 5152 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2489 2489 Size: 5971 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 5118 1678 Size: 3440 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2519 2551 4612 4644 4686 Size: 32 135 32 947 32 2061 32 42 Cuts at: 4686 4718 1373 Size: 32 2626 Fragments arranged by size: 2626 2061 947 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5085 5269 1305 Size: 3780 184 2007 Fragments arranged by size: 3780 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4149 Fragments arranged by size: 4149 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2556 2684 2782 3929 4288 4447 518 Size: 648 1390 128 98 1147 359 159 2042 Fragments arranged by size: 2042 1390 1147 648 359 159 128 98 MspA1I CmG'CkG Cuts at: 1606 2089 3091 3387 4064 5212 5457 1606 Size: 483 1002 296 677 1148 245 2120 Fragments arranged by size: 2120 1148 1002 677 483 296 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4480 Fragments arranged by size: 4480 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NsiI A_TGCA'T Cuts at: 2710 2710 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 2243 2712 3048 3329 4874 1132 Size: 97 1014 469 336 281 1545 2229 Fragments arranged by size: 2229 1545 1014 469 336 281 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2253 2285 4040 4072 5553 5585 2253 Size: 32 1755 32 1481 32 2639 Fragments arranged by size: 2639 1755 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2106 2925 2106 Size: 819 5152 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PstI C_TGCA'G Cuts at: 830 3252 3323 830 Size: 2422 71 3478 Fragments arranged by size: 3478 2422 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2089 2089 Size: 5971 SacI G_AGCT'C Cuts at: 2735 2735 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 3248 3319 4454 5135 5326 826 Size: 219 2203 71 1135 681 191 1471 Fragments arranged by size: 2203 1471 1135 681 219 191 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 2072 3298 1872 Size: 200 1226 4545 Fragments arranged by size: 4545 1226 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 2356 2789 4075 4976 5238 820 Size: 197 926 413 433 1286 901 262 1553 Fragments arranged by size: 1553 1286 926 901 433 413 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 2232 2804 2888 3056 3224 1517 Size: 715 572 84 168 168 4264 Fragments arranged by size: 4264 715 572 168 168 84 StyI C'CwwG_G Cuts at: 513 1065 2004 3410 513 Size: 552 939 1406 3074 Fragments arranged by size: 3074 1406 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2909 3140 3998 4015 4168 4353 4798 Size: 1255 1329 231 858 17 153 185 445 Cuts at: 4798 325 Size: 1498 Fragments arranged by size: 1498 1329 1255 858 445 231 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4217 5929 96 Size: 274 80 33 51 1443 2240 1712 138 Fragments arranged by size: 2240 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5931 5963 5931 Size: 32 5939 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 2179 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 2070 3296 1870 Size: 200 1226 4545 Fragments arranged by size: 4545 1226 200 XmnI GAAnn'nnTTC Cuts at: 2804 3224 4100 2804 Size: 420 876 4675 Fragments arranged by size: 4675 876 420 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI