(Circular) (Six-base) MAPSORT of: pFN21AA0408.seq Check: 2232 from: 1 to: 7546 pFN21AA0408 7546 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3957 3957 Size: 7546 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5356 278 Size: 53 83 186 936 3820 2468 Fragments arranged by size: 3820 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4889 1937 Size: 2952 4594 Acc65I G'GTAC_C Cuts at: 4867 4867 Size: 7546 AclI AA'CG_TT Cuts at: 1563 5673 6046 1563 Size: 4110 373 3063 Fragments arranged by size: 4110 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2397 3599 3869 4139 5609 6993 7261 1632 Size: 765 1202 270 270 1470 1384 268 1917 Fragments arranged by size: 1917 1470 1384 1202 765 270 270 268 AfeI AGC'GCT Cuts at: 4208 4208 Size: 7546 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7349 AflIII A'CryG_T Cuts at: 1203 6445 7422 1203 Size: 5242 977 1327 Fragments arranged by size: 5242 1327 977 AgeI A'CCGG_T Cuts at: 6377 6377 Size: 7546 AhdI GACnn_n'nnGTC Cuts at: 6275 6275 Size: 7546 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7546 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3536 4178 4673 1184 Size: 2352 642 495 4057 Fragments arranged by size: 4057 2352 642 495 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3657 3689 3657 Size: 32 7514 AlwNI CAG_nnn'CTG Cuts at: 2072 2227 2589 3949 5289 6861 2072 Size: 155 362 1360 1340 1572 2757 Fragments arranged by size: 2757 1572 1360 1340 362 155 ApaI G_GGCC'C Cuts at: 2957 4332 4416 2957 Size: 1375 84 6087 Fragments arranged by size: 6087 1375 84 ApaLI G'TGCA_C Cuts at: 5603 6759 5603 Size: 1156 6390 ApoI r'AATT_y Cuts at: 1493 4855 5081 5233 7342 1493 Size: 3362 226 152 2109 1697 Fragments arranged by size: 3362 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 4127 4127 Size: 7546 AseI AT'TA_AT Cuts at: 160 1032 6100 160 Size: 872 5068 1606 Fragments arranged by size: 5068 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 AvaI C'yCGr_G Cuts at: 1870 1943 2432 2445 2474 4409 4679 4871 1870 Size: 73 489 13 29 1935 270 192 4545 Fragments arranged by size: 4545 1935 489 270 192 73 29 13 BamHI G'GATC_C Cuts at: 3225 4876 5243 3225 Size: 1651 367 5528 Fragments arranged by size: 5528 1651 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2329 4077 4405 4684 4709 Size: 325 217 627 542 1748 328 279 25 Cuts at: 4709 4867 6322 618 Size: 158 1455 1842 Fragments arranged by size: 1842 1748 1455 627 542 328 325 279 217 158 25 BanII G_rGCy'C Cuts at: 1391 2122 2781 2957 3675 3765 4035 4149 4332 Size: 731 659 176 718 90 270 114 183 Cuts at: 4332 4416 4722 4865 1391 Size: 84 306 143 4072 Fragments arranged by size: 4072 731 718 659 306 270 183 176 143 114 90 84 BbeI G_GCGC'C Cuts at: 2333 4409 4713 2333 Size: 2076 304 5166 Fragments arranged by size: 5166 2076 304 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3969 4810 928 Size: 971 2070 841 3664 Fragments arranged by size: 3664 2070 971 841 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3149 3183 3799 3833 4471 4505 5737 Size: 34 1212 34 616 34 638 34 1232 Cuts at: 5737 5771 1903 Size: 34 3678 Fragments arranged by size: 3678 1232 1212 638 616 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5438 6648 5438 Size: 1210 6336 BclI T'GATC_A Cuts at: 1548 1548 Size: 7546 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 2315 6157 Size: 107 122 71 631 605 403 240 3842 Cuts at: 6157 136 Size: 1525 Fragments arranged by size: 3842 1525 631 605 403 240 122 107 71 BglII A'GATC_T Cuts at: 3574 7541 3574 Size: 3967 3579 BlpI GC'TnA_GC Cuts at: 2613 4148 4963 2613 Size: 1535 815 5196 Fragments arranged by size: 5196 1535 815 Bme1580I G_kGCm'C Cuts at: 1790 2466 2957 4332 4416 4689 5607 6763 1790 Size: 676 491 1375 84 273 918 1156 2573 Fragments arranged by size: 2573 1375 1156 918 676 491 273 84 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7546 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2177 5277 6235 Size: 416 53 83 399 24 760 3100 958 Cuts at: 6235 442 Size: 1753 Fragments arranged by size: 3100 1753 958 760 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7546 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2662 4366 2662 Size: 1704 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3335 3659 3848 4022 6206 1239 Size: 2096 324 189 174 2184 2579 Fragments arranged by size: 2579 2184 2096 324 189 174 Bpu10I CC'TnA_GC Cuts at: 3776 3776 Size: 7546 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2780 3086 3311 3749 5671 6536 6834 2780 Size: 306 225 438 1922 865 298 3492 Fragments arranged by size: 3492 1922 865 438 306 298 225 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2900 6209 882 Size: 617 1401 3309 2219 Fragments arranged by size: 3309 2219 1401 617 BsaAI yAC'GTr Cuts at: 493 1204 4543 5337 493 Size: 711 3339 794 2702 Fragments arranged by size: 3339 2702 794 711 BsaBI GATnn'nnATC Cuts at: 7540 7540 Size: 7546 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2321 2330 4260 4406 Size: 53 83 186 936 788 9 1930 146 Cuts at: 4406 4710 5353 5735 6370 275 Size: 304 643 382 635 1451 Fragments arranged by size: 1930 1451 936 788 643 635 382 304 186 146 83 53 9 BsaWI w'CCGG_w Cuts at: 2705 5979 6377 6651 6798 7209 2705 Size: 3274 398 274 147 411 3042 Fragments arranged by size: 3274 3042 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2441 2471 3657 3687 1178 Size: 30 165 30 1038 30 1186 30 5037 Fragments arranged by size: 5037 1186 1038 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2307 2450 2520 2538 2541 2653 3080 3176 Size: 1121 143 70 18 3 112 427 96 Cuts at: 3176 3575 4019 4127 4370 4592 4730 1186 Size: 399 444 108 243 222 138 4002 Fragments arranged by size: 4002 1121 444 427 399 243 222 143 138 112 108 96 70 18 3 BseYI C'CCAG_C Cuts at: 2958 3743 6749 7245 2958 Size: 785 3006 496 3259 Fragments arranged by size: 3259 3006 785 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 1163 Size: 573 333 6640 Fragments arranged by size: 6640 573 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2826 4438 4495 4675 4705 4996 Size: 275 186 825 1612 57 180 30 291 Cuts at: 4996 5757 6785 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1612 1028 825 761 291 275 186 180 57 30 BsiHKAI G_wGCw'C Cuts at: 2122 2781 3675 3765 4035 4149 4517 4608 5607 Size: 659 894 90 270 114 368 91 999 Cuts at: 5607 5692 6763 2122 Size: 85 1071 2905 Fragments arranged by size: 2905 1071 999 894 659 368 270 114 91 90 85 BsiWI C'GTAC_G Cuts at: 2605 2605 Size: 7546 BsmI GAATG_Cn' Cuts at: 5057 5150 5057 Size: 93 7453 BsmBI CGTCTCn'nnnn_ Cuts at: 3962 3962 Size: 7546 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2122 2466 2781 2957 3675 3765 4035 Size: 399 332 344 315 176 718 90 270 Cuts at: 4035 4149 4332 4416 4517 4608 4689 4722 4865 Size: 114 183 84 101 91 81 33 143 Cuts at: 4865 5607 5692 6763 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 718 399 344 332 315 270 183 176 143 114 101 91 90 85 84 81 33 BspEI T'CCGG_A Cuts at: 2705 7209 2705 Size: 4504 3042 BspHI T'CATG_A Cuts at: 3906 5435 7165 3906 Size: 1529 1730 4287 Fragments arranged by size: 4287 1730 1529 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3957 4901 844 Size: 3113 944 3489 Fragments arranged by size: 3489 3113 944 BsrBI CCG'CTC Cuts at: 1394 1823 2345 2491 2806 2985 3904 4461 5433 Size: 429 522 146 315 179 919 557 972 Cuts at: 5433 1394 Size: 3507 Fragments arranged by size: 3507 972 919 557 522 429 315 179 146 BsrDI GCAATG_nn' Cuts at: 65 1248 6041 6215 65 Size: 1183 4793 174 1396 Fragments arranged by size: 4793 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2192 2531 2690 4494 6190 6377 1700 Size: 110 382 339 159 1804 1696 187 2869 Fragments arranged by size: 2869 1804 1696 382 339 187 159 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7546 BssHII G'CGCG_C Cuts at: 1925 4071 4127 4532 1925 Size: 2146 56 405 4939 Fragments arranged by size: 4939 2146 405 56 BssSI C'ACGA_G Cuts at: 3194 3668 4510 4607 5606 6618 3194 Size: 474 842 97 999 1012 4122 Fragments arranged by size: 4122 1012 999 842 474 97 BstAPI GCAn_nnn'nTGC Cuts at: 794 2227 3187 3949 4612 794 Size: 1433 960 762 663 3728 Fragments arranged by size: 3728 1433 960 762 663 BstBI TT'CG_AA Cuts at: 6350 6350 Size: 7546 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7546 BstXI CCAn_nnnn'nTGG Cuts at: 3805 3805 Size: 7546 BstYI r'GATC_y Cuts at: 1972 3225 3574 3686 3700 4876 5243 5628 5645 Size: 1253 349 112 14 1176 367 385 17 Cuts at: 5645 7097 7183 7195 7541 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1253 1176 385 367 349 346 112 86 17 14 12 Bsu36I CC'TnA_GG Cuts at: 3869 3869 Size: 7546 BtgI C'CryG_G Cuts at: 513 1065 2826 4237 4705 513 Size: 552 1761 1411 468 3354 Fragments arranged by size: 3354 1761 1411 552 468 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3891 4486 487 Size: 683 56 2665 595 3547 Fragments arranged by size: 3547 2665 683 595 56 BtsI GCAGTG_nn' Cuts at: 5062 5856 5876 5062 Size: 794 20 6732 Fragments arranged by size: 6732 794 20 ClaI AT'CG_AT Cuts at: 1554 3114 1554 Size: 1560 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7511 DraI TTT'AAA Cuts at: 4810 4850 4810 Size: 40 7506 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6553 809 Size: 507 5237 1802 Fragments arranged by size: 5237 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2169 2400 2823 Size: 54 1425 50 275 18 339 231 423 Cuts at: 2823 2913 3633 3894 3993 4060 4545 4993 5882 Size: 90 720 261 99 67 485 448 889 Cuts at: 5882 7511 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 720 485 448 423 339 275 261 231 99 90 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2823 4993 1537 Size: 275 1011 2170 4090 Fragments arranged by size: 4090 2170 1011 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2671 3499 5476 1353 Size: 1318 828 1977 3423 Fragments arranged by size: 3423 1977 1318 828 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2313 2914 2963 3869 4043 6133 Size: 1064 138 724 601 49 906 174 2090 Cuts at: 6133 6507 6653 387 Size: 374 146 1280 Fragments arranged by size: 2090 1280 1064 906 724 601 374 174 146 138 49 EcoICRI GAG'CTC Cuts at: 2120 2779 3673 3763 4033 4147 2120 Size: 659 894 90 270 114 5519 Fragments arranged by size: 5519 894 659 270 114 90 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2397 3335 3599 3659 3848 3869 4022 Size: 393 765 938 264 60 189 21 153 Cuts at: 4022 4139 5609 6206 6993 7261 1239 Size: 117 1470 597 787 268 1524 Fragments arranged by size: 1524 1470 938 787 765 597 393 268 264 189 153 117 60 21 EcoNI CCTnn'n_nnAGG Cuts at: 3317 4109 3317 Size: 792 6754 EcoO109I rG'GnC_Cy Cuts at: 2643 2953 2954 3322 3737 4102 4328 4413 4555 Size: 310 1 368 415 365 226 85 142 Cuts at: 4555 2643 Size: 5634 Fragments arranged by size: 5634 415 368 365 310 226 142 85 1 EcoRI G'AATT_C Cuts at: 4855 5233 4855 Size: 378 7168 FspI TGC'GCA Cuts at: 3308 4364 6052 3308 Size: 1056 1688 4802 Fragments arranged by size: 4802 1688 1056 HaeII r_GCGC'y Cuts at: 1678 2031 2333 2565 3011 4210 4409 4477 4713 Size: 353 302 232 446 1199 199 68 236 Cuts at: 4713 6693 1678 Size: 1980 2531 Fragments arranged by size: 2531 1980 1199 446 353 302 236 232 199 68 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2148 2180 2441 2473 4205 Size: 32 135 32 576 32 261 32 1732 Cuts at: 4205 4237 4283 4315 4445 4477 6187 6219 6261 Size: 32 46 32 130 32 1710 32 42 Cuts at: 6261 6293 1373 Size: 32 2626 Fragments arranged by size: 2626 1732 1710 576 261 135 130 46 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3176 4890 5136 669 Size: 988 281 1238 1714 246 3079 Fragments arranged by size: 3079 1714 1238 988 281 246 HindIII A'AGCT_T Cuts at: 748 3456 748 Size: 2708 4838 HpaI GTT'AAC Cuts at: 5136 5136 Size: 7546 KasI G'GCGC_C Cuts at: 2329 4405 4709 2329 Size: 2076 304 5166 Fragments arranged by size: 5166 2076 304 KpnI G_GTAC'C Cuts at: 4871 4871 Size: 7546 MfeI C'AATT_G Cuts at: 5145 5145 Size: 7546 MluI A'CGCG_T Cuts at: 7422 7422 Size: 7546 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3180 3912 4221 6660 6844 1305 Size: 1875 732 309 2439 184 2007 Fragments arranged by size: 2439 2007 1875 732 309 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3635 4547 10 Size: 54 1425 343 1803 912 3009 Fragments arranged by size: 3009 1803 1425 912 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4541 4727 5504 5863 6022 518 Size: 648 3375 186 777 359 159 2042 Fragments arranged by size: 3375 2042 777 648 359 186 159 MspA1I CmG'CkG Cuts at: 1606 2063 2227 2631 2733 2816 2828 3589 3955 Size: 457 164 404 102 83 12 761 366 Cuts at: 3955 4152 4168 4239 4374 4707 4962 5639 6787 Size: 197 16 71 135 333 255 677 1148 Cuts at: 6787 7032 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 761 677 457 404 366 333 255 245 197 164 135 102 83 71 16 12 NaeI GCC'GGC Cuts at: 2194 2533 2194 Size: 339 7207 NarI GG'CG_CC Cuts at: 2330 4406 4710 2330 Size: 2076 304 5166 Fragments arranged by size: 5166 2076 304 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6994 NdeI CA'TA_TG Cuts at: 387 387 Size: 7546 NgoMIV G'CCGG_C Cuts at: 2192 2531 2192 Size: 339 7207 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7546 NotI GC'GGCC_GC Cuts at: 2823 4993 2823 Size: 2170 5376 NspI r_CATG'y Cuts at: 1132 1229 2998 4204 4904 6449 1132 Size: 97 1769 1206 700 1545 2229 Fragments arranged by size: 2229 1769 1545 1206 700 97 PasI CC'CwG_GG Cuts at: 1348 2583 1348 Size: 1235 6311 PciI A'CATG_T Cuts at: 6445 6445 Size: 7546 PflMI CCAn_nnn'nTGG Cuts at: 1108 2365 2589 3199 1108 Size: 1257 224 610 5455 Fragments arranged by size: 5455 1257 610 224 PfoI T'CCnGG_A Cuts at: 2347 4599 2347 Size: 2252 5294 PmeI GTTT'AAAC Cuts at: 4850 4850 Size: 7546 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2131 2163 3657 3689 5615 5647 7128 7160 2131 Size: 32 1494 32 1926 32 1481 32 2517 Fragments arranged by size: 2517 1926 1494 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3322 4555 3322 Size: 1233 6313 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7546 PsiI TTA'TAA Cuts at: 5116 5116 Size: 7546 PspOMI G'GGCC_C Cuts at: 2953 4328 4412 2953 Size: 1375 84 6087 Fragments arranged by size: 6087 1375 84 PspXI vC'TCGA_Gb Cuts at: 4679 4679 Size: 7546 PstI C_TGCA'G Cuts at: 830 2068 2232 2772 3183 3234 3594 3954 4110 Size: 1238 164 540 411 51 360 360 156 Cuts at: 4110 4137 4173 4662 4898 830 Size: 27 36 489 236 3478 Fragments arranged by size: 3478 1238 540 489 411 360 360 236 164 156 51 36 27 PvuI CG_AT'CG Cuts at: 2001 4438 2001 Size: 2437 5109 PvuII CAG'CTG Cuts at: 2227 3589 3955 4168 2227 Size: 1362 366 213 5605 Fragments arranged by size: 5605 1362 366 213 RsrII CG'GwC_CG Cuts at: 2426 2426 Size: 7546 SacI G_AGCT'C Cuts at: 2122 2781 3675 3765 4035 4149 2122 Size: 659 894 90 270 114 5519 Fragments arranged by size: 5519 894 659 270 114 90 SacII CC_GC'GG Cuts at: 2829 4240 4708 2829 Size: 1411 468 5667 Fragments arranged by size: 5667 1411 468 SalI G'TCGA_C Cuts at: 1936 4888 1936 Size: 2952 4594 SbfI CC_TGCA'GG Cuts at: 4110 4898 4110 Size: 788 6758 ScaI AGT'ACT Cuts at: 5794 7506 5794 Size: 1712 5834 SfcI C'TryA_G Cuts at: 826 1045 2064 2228 2768 3179 3230 3590 3950 Size: 219 1019 164 540 411 51 360 360 Cuts at: 3950 4106 4133 4169 4658 4894 6029 6710 6901 Size: 156 27 36 489 236 1135 681 191 Cuts at: 6901 826 Size: 1471 Fragments arranged by size: 1471 1135 1019 681 540 489 411 360 360 236 219 191 164 156 51 36 27 SfoI GGC'GCC Cuts at: 2331 4407 4711 2331 Size: 2076 304 5166 Fragments arranged by size: 5166 2076 304 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7546 SmaI CCC'GGG Cuts at: 1872 4411 4873 1872 Size: 2539 462 4545 Fragments arranged by size: 4545 2539 462 SmlI C'TyrA_G Cuts at: 820 1017 1943 2795 3101 3326 3764 4679 5650 Size: 197 926 852 306 225 438 915 971 Cuts at: 5650 6551 6813 820 Size: 901 262 1553 Fragments arranged by size: 1553 971 926 915 901 852 438 306 262 225 197 SnaBI TAC'GTA Cuts at: 493 5337 493 Size: 4844 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7546 SphI G_CATG'C Cuts at: 1132 2998 4904 1132 Size: 1866 1906 3774 Fragments arranged by size: 3774 1906 1866 SrfI GCCC'GGGC Cuts at: 4411 4411 Size: 7546 SspI AAT'ATT Cuts at: 5 52 4806 5470 5 Size: 47 4754 664 2081 Fragments arranged by size: 4754 2081 664 47 StuI AGG'CCT Cuts at: 1517 3846 4643 1517 Size: 2329 797 4420 Fragments arranged by size: 4420 2329 797 StyI C'CwwG_G Cuts at: 513 1065 4985 513 Size: 552 3920 3074 Fragments arranged by size: 3920 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2152 4112 5573 5590 5743 5928 6373 Size: 1255 572 1960 1461 17 153 185 445 Cuts at: 6373 325 Size: 1498 Fragments arranged by size: 1960 1498 1461 1255 572 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3681 4041 4047 Size: 274 80 33 51 1443 1704 360 6 Cuts at: 4047 4840 5792 7504 96 Size: 793 952 1712 138 Fragments arranged by size: 1712 1704 1443 952 793 360 274 138 80 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7506 7538 7506 Size: 32 7514 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7546 XbaI T'CTAG_A Cuts at: 4882 4882 Size: 7546 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2366 2366 Size: 7546 XhoI C'TCGA_G Cuts at: 1943 4679 1943 Size: 2736 4810 XmaI C'CCGG_G Cuts at: 1870 4409 4871 1870 Size: 2539 462 4545 Fragments arranged by size: 4545 2539 462 XmnI GAAnn'nnTTC Cuts at: 4643 5675 4643 Size: 1032 6514 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5354 276 Size: 53 83 186 936 3820 2468 Fragments arranged by size: 3820 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AvrII BaeI BbvCI BfrBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI SanDI SapI SexAI SfiI SwaI