(Circular) (Six-base) MAPSORT of: pFN21AE0138.seq Check: 5405 from: 1 to: 5515 pFN21AE0138 5515 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2196 2196 Size: 5515 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2858 1937 Size: 921 4594 Acc65I G'GTAC_C Cuts at: 2836 2836 Size: 5515 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3578 4962 5230 1632 Size: 1946 1384 268 1917 Fragments arranged by size: 1946 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 4414 5391 1203 Size: 3211 977 1327 Fragments arranged by size: 3211 1327 977 AgeI A'CCGG_T Cuts at: 4346 4346 Size: 5515 AhdI GACnn_n'nnGTC Cuts at: 4244 4244 Size: 5515 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5515 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5515 AlwNI CAG_nnn'CTG Cuts at: 2020 2509 2529 3258 4830 2020 Size: 489 20 729 1572 2705 Fragments arranged by size: 2705 1572 729 489 20 ApaI G_GGCC'C Cuts at: 2790 2790 Size: 5515 ApaLI G'TGCA_C Cuts at: 3572 4728 3572 Size: 1156 4359 ApoI r'AATT_y Cuts at: 1493 2824 3050 3202 5311 1493 Size: 1331 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 226 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2162 2781 2840 1870 Size: 73 219 619 59 4545 Fragments arranged by size: 4545 619 219 73 59 BamHI G'GATC_C Cuts at: 2845 3212 2845 Size: 367 5148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2167 2281 2836 4291 618 Size: 325 217 627 380 114 555 1455 1842 Fragments arranged by size: 1842 1455 627 555 380 325 217 114 BanII G_rGCy'C Cuts at: 1391 2790 2834 1391 Size: 1399 44 4072 Fragments arranged by size: 4072 1399 44 BbeI G_GCGC'C Cuts at: 2171 2171 Size: 5515 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2550 928 Size: 971 651 3893 Fragments arranged by size: 3893 971 651 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3706 3740 1903 Size: 34 50 34 1685 34 3678 Fragments arranged by size: 3678 1685 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 4617 3407 Size: 1210 4305 BclI T'GATC_A Cuts at: 1548 1548 Size: 5515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2120 2217 4126 Size: 107 122 71 631 605 448 97 1909 Cuts at: 4126 136 Size: 1525 Fragments arranged by size: 1909 1525 631 605 448 122 107 97 71 BglII A'GATC_T Cuts at: 5510 5510 Size: 5515 BlpI GC'TnA_GC Cuts at: 2932 2932 Size: 5515 Bme1580I G_kGCm'C Cuts at: 1790 2286 2790 3576 4732 1790 Size: 496 504 786 1156 2573 Fragments arranged by size: 2573 1156 786 504 496 BmgBI CAC'GTC Cuts at: 1318 2602 1318 Size: 1284 4231 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2539 3246 4204 Size: 416 53 83 399 24 1122 707 958 Cuts at: 4204 442 Size: 1753 Fragments arranged by size: 1753 1122 958 707 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2278 2297 2506 2654 2738 4175 1239 Size: 1039 19 209 148 84 1437 2579 Fragments arranged by size: 2579 1437 1039 209 148 84 19 Bpu10I CC'TnA_GC Cuts at: 2096 2096 Size: 5515 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2430 3640 4505 4803 2430 Size: 1210 865 298 3142 Fragments arranged by size: 3142 1210 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2120 4178 882 Size: 617 621 2058 2219 Fragments arranged by size: 2219 2058 621 617 BsaAI yAC'GTr Cuts at: 493 1204 3306 493 Size: 711 2102 2702 Fragments arranged by size: 2702 2102 711 BsaBI GATnn'nnATC Cuts at: 5509 5509 Size: 5515 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2168 3322 3704 4339 Size: 53 83 186 936 635 1154 382 635 Cuts at: 4339 275 Size: 1451 Fragments arranged by size: 1451 1154 936 635 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3948 4346 4620 4767 5178 3948 Size: 398 274 147 411 4285 Fragments arranged by size: 4285 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5290 Fragments arranged by size: 5290 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2554 2603 2606 1186 Size: 1368 49 3 4095 Fragments arranged by size: 4095 1368 49 3 BseYI C'CCAG_C Cuts at: 2050 4718 5214 2050 Size: 2668 496 2351 Fragments arranged by size: 2668 2351 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2141 2591 1163 Size: 573 405 450 4087 Fragments arranged by size: 4087 573 450 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2235 2965 3726 4754 1540 Size: 275 186 234 730 761 1028 2301 Fragments arranged by size: 2301 1028 761 730 275 234 186 BsiHKAI G_wGCw'C Cuts at: 2564 3576 3661 4732 2564 Size: 1012 85 1071 3347 Fragments arranged by size: 3347 1071 1012 85 BsmI GAATG_Cn' Cuts at: 3026 3119 3026 Size: 93 5422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2286 2564 2790 2834 3576 3661 4732 Size: 399 496 278 226 44 742 85 1071 Cuts at: 4732 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 496 399 278 226 85 44 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 3404 5134 3404 Size: 1730 3785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2196 2814 2870 844 Size: 1352 618 56 3489 Fragments arranged by size: 3489 1352 618 56 BsrBI CCG'CTC Cuts at: 1394 1823 3402 1394 Size: 429 1579 3507 Fragments arranged by size: 3507 1579 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4010 4184 65 Size: 1183 2762 174 1396 Fragments arranged by size: 2762 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2207 2288 2309 2646 4159 4346 1700 Size: 110 397 81 21 337 1513 187 2869 Fragments arranged by size: 2869 1513 397 337 187 110 81 21 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5515 BssHII G'CGCG_C Cuts at: 1925 2060 2062 2760 1925 Size: 135 2 698 4680 Fragments arranged by size: 4680 698 135 2 BssSI C'ACGA_G Cuts at: 2326 3575 4587 2326 Size: 1249 1012 3254 Fragments arranged by size: 3254 1249 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5515 BstBI TT'CG_AA Cuts at: 4319 4319 Size: 5515 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5515 BstXI CCAn_nnnn'nTGG Cuts at: 2149 2149 Size: 5515 BstYI r'GATC_y Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510 Size: 873 367 385 17 1452 86 12 346 Cuts at: 5510 1972 Size: 1977 Fragments arranged by size: 1977 1452 873 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2375 2375 Size: 5515 BtgI C'CryG_G Cuts at: 513 1065 2343 513 Size: 552 1278 3685 Fragments arranged by size: 3685 1278 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4776 Fragments arranged by size: 4776 683 56 BtsI GCAGTG_nn' Cuts at: 3031 3825 3845 3031 Size: 794 20 4701 Fragments arranged by size: 4701 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5480 DraI TTT'AAA Cuts at: 2819 2819 Size: 5515 DraIII CAC_nnn'GTG Cuts at: 2344 2344 Size: 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4522 809 Size: 507 3206 1802 Fragments arranged by size: 3206 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2209 2232 Size: 54 1425 50 275 18 291 88 23 Cuts at: 2232 2625 2962 3851 5480 8 Size: 393 337 889 1629 43 Fragments arranged by size: 1629 1425 889 393 337 291 275 88 54 50 43 23 18 EagI C'GGCC_G Cuts at: 1537 1812 2232 2962 1537 Size: 275 420 730 4090 Fragments arranged by size: 4090 730 420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2228 3445 1353 Size: 875 1217 3423 Fragments arranged by size: 3423 1217 875 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4102 4476 4622 387 Size: 1064 138 2513 374 146 1280 Fragments arranged by size: 2513 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2278 2297 2506 2654 2738 3578 4175 Size: 393 646 19 209 148 84 840 597 Cuts at: 4175 4962 5230 1239 Size: 787 268 1524 Fragments arranged by size: 1524 840 787 646 597 393 268 209 148 84 19 EcoNI CCTnn'n_nnAGG Cuts at: 2099 2099 Size: 5515 EcoO109I rG'GnC_Cy Cuts at: 2045 2091 2218 2682 2786 2045 Size: 46 127 464 104 4774 Fragments arranged by size: 4774 464 127 104 46 EcoRI G'AATT_C Cuts at: 2824 3202 2824 Size: 378 5137 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2701 2701 Size: 5515 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 2057 2156 2171 4662 1678 Size: 379 99 15 2491 2531 Fragments arranged by size: 2531 2491 379 99 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4156 4188 4230 4262 1373 Size: 32 135 32 2584 32 42 32 2626 Fragments arranged by size: 2626 2584 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2859 3105 669 Size: 988 281 921 246 3079 Fragments arranged by size: 3079 988 921 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5515 HpaI GTT'AAC Cuts at: 3105 3105 Size: 5515 KasI G'GCGC_C Cuts at: 2167 2167 Size: 5515 KpnI G_GTAC'C Cuts at: 2840 2840 Size: 5515 MfeI C'AATT_G Cuts at: 3114 3114 Size: 5515 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4629 4813 1305 Size: 3324 184 2007 Fragments arranged by size: 3324 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2627 10 Size: 54 1425 343 795 2898 Fragments arranged by size: 2898 1425 795 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3473 3832 3991 518 Size: 648 2307 359 159 2042 Fragments arranged by size: 2307 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2074 2509 2931 3608 4756 5001 1606 Size: 411 57 435 422 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 435 422 411 245 57 NaeI GCC'GGC Cuts at: 2209 2290 2209 Size: 81 5434 NarI GG'CG_CC Cuts at: 2168 2168 Size: 5515 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4963 NdeI CA'TA_TG Cuts at: 387 387 Size: 5515 NgoMIV G'CCGG_C Cuts at: 2207 2288 2207 Size: 81 5434 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5515 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2275 2873 4418 1132 Size: 97 1046 598 1545 2229 Fragments arranged by size: 2229 1545 1046 598 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5515 PciI A'CATG_T Cuts at: 4414 4414 Size: 5515 PflMI CCAn_nnn'nTGG Cuts at: 1108 2344 2529 1108 Size: 1236 185 4094 Fragments arranged by size: 4094 1236 185 PfoI T'CCnGG_A Cuts at: 2349 2538 2655 2349 Size: 189 117 5209 Fragments arranged by size: 5209 189 117 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 5097 5129 3584 Size: 32 1481 32 3970 Fragments arranged by size: 3970 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2045 2682 2045 Size: 637 4878 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5515 PsiI TTA'TAA Cuts at: 2394 3085 2394 Size: 691 4824 PspOMI G'GGCC_C Cuts at: 2786 2786 Size: 5515 PstI C_TGCA'G Cuts at: 830 2616 2867 830 Size: 1786 251 3478 Fragments arranged by size: 3478 1786 251 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5515 PvuII CAG'CTG Cuts at: 2017 2074 2509 2017 Size: 57 435 5023 Fragments arranged by size: 5023 435 57 SalI G'TCGA_C Cuts at: 1936 2857 1936 Size: 921 4594 SanDI GG'GwC_CC Cuts at: 2045 2045 Size: 5515 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 3763 5475 3763 Size: 1712 3803 SfcI C'TryA_G Cuts at: 826 1045 2612 2863 3998 4679 4870 826 Size: 219 1567 251 1135 681 191 1471 Fragments arranged by size: 1567 1471 1135 681 251 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2217 2217 Size: 5515 SfoI GGC'GCC Cuts at: 2169 2169 Size: 5515 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2783 2842 1872 Size: 911 59 4545 Fragments arranged by size: 4545 911 59 SmlI C'TyrA_G Cuts at: 820 1017 1943 2445 3619 4520 4782 820 Size: 197 926 502 1174 901 262 1553 Fragments arranged by size: 1553 1174 926 901 502 262 197 SnaBI TAC'GTA Cuts at: 493 3306 493 Size: 2813 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2275 2873 1132 Size: 1143 598 3774 Fragments arranged by size: 3774 1143 598 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 2579 1517 Size: 1062 4453 StyI C'CwwG_G Cuts at: 513 1065 2954 513 Size: 552 1889 3074 Fragments arranged by size: 3074 1889 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3542 3559 3712 3897 4342 325 Size: 1255 1962 17 153 185 445 1498 Fragments arranged by size: 1962 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3761 5473 96 Size: 274 80 33 51 1443 1784 1712 138 Fragments arranged by size: 1784 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5475 5507 5475 Size: 32 5483 Tth111I GACn'n_nGTC Cuts at: 1316 2600 1316 Size: 1284 4231 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5515 XmaI C'CCGG_G Cuts at: 1870 2781 2840 1870 Size: 911 59 4545 Fragments arranged by size: 4545 911 59 XmnI GAAnn'nnTTC Cuts at: 3644 3644 Size: 5515 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SrfI SwaI XcmI