(Circular) (Six-base) MAPSORT of: pFN21AB9116.seq Check: 1907 from: 1 to: 7021 pFN21AB9116 7021 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4831 278 Size: 53 83 186 936 3295 2468 Fragments arranged by size: 3295 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4364 1937 Size: 2427 4594 Acc65I G'GTAC_C Cuts at: 4342 4342 Size: 7021 AclI AA'CG_TT Cuts at: 1563 3197 5148 5521 1563 Size: 1634 1951 373 3063 Fragments arranged by size: 3063 1951 1634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4125 4161 5084 6468 6736 1632 Size: 2493 36 923 1384 268 1917 Fragments arranged by size: 2493 1917 1384 923 268 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6824 AflIII A'CryG_T Cuts at: 1203 5920 6897 1203 Size: 4717 977 1327 Fragments arranged by size: 4717 1327 977 AgeI A'CCGG_T Cuts at: 5852 5852 Size: 7021 AhdI GACnn_n'nnGTC Cuts at: 5750 5750 Size: 7021 AleI CACnn'nnGTG Cuts at: 1166 3943 1166 Size: 2777 4244 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7021 AlwNI CAG_nnn'CTG Cuts at: 4207 4764 6336 4207 Size: 557 1572 4892 Fragments arranged by size: 4892 1572 557 ApaLI G'TGCA_C Cuts at: 5078 6234 5078 Size: 1156 5865 ApoI r'AATT_y Cuts at: 1493 2677 2989 3477 4330 4556 4708 6817 1493 Size: 1184 312 488 853 226 152 2109 1697 Fragments arranged by size: 2109 1697 1184 853 488 312 226 152 AseI AT'TA_AT Cuts at: 160 1032 3686 5575 160 Size: 872 2654 1889 1606 Fragments arranged by size: 2654 1889 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7021 AvaI C'yCGr_G Cuts at: 1870 1943 2162 4346 1870 Size: 73 219 2184 4545 Fragments arranged by size: 4545 2184 219 73 AvrII C'CTAG_G Cuts at: 2240 2240 Size: 7021 BamHI G'GATC_C Cuts at: 2132 4351 4718 2132 Size: 2219 367 4435 Fragments arranged by size: 4435 2219 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4342 5797 618 Size: 325 217 627 2555 1455 1842 Fragments arranged by size: 2555 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3178 4340 1391 Size: 1787 1162 4072 Fragments arranged by size: 4072 1787 1162 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2097 928 Size: 971 198 5852 Fragments arranged by size: 5852 971 198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5212 5246 1903 Size: 34 3275 34 3678 Fragments arranged by size: 3678 3275 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3148 3327 3604 4913 6123 3148 Size: 179 277 1309 1210 4046 Fragments arranged by size: 4046 1309 1210 277 179 BclI T'GATC_A Cuts at: 1548 1548 Size: 7021 BfrBI ATG'CAT Cuts at: 3536 3536 Size: 7021 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5632 136 Size: 107 122 71 631 605 3960 1525 Fragments arranged by size: 3960 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2604 3975 7016 2604 Size: 1371 3041 2609 Fragments arranged by size: 3041 2609 1371 BlpI GC'TnA_GC Cuts at: 2538 4438 2538 Size: 1900 5121 Bme1580I G_kGCm'C Cuts at: 1790 5082 6238 1790 Size: 3292 1156 2573 Fragments arranged by size: 3292 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7021 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2623 2900 4752 Size: 416 53 83 399 24 1206 277 1852 Cuts at: 4752 5710 442 Size: 958 1753 Fragments arranged by size: 1852 1753 1206 958 416 399 277 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2468 1056 Size: 1412 5609 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2531 2531 Size: 7021 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2088 3305 3520 4290 5681 1239 Size: 849 1217 215 770 1391 2579 Fragments arranged by size: 2579 1391 1217 849 770 215 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2158 2905 5146 6011 6309 2158 Size: 747 2241 865 298 2870 Fragments arranged by size: 2870 2241 865 747 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5684 882 Size: 617 4185 2219 Fragments arranged by size: 4185 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3466 4812 493 Size: 711 2262 1346 2702 Fragments arranged by size: 2702 2262 1346 711 BsaBI GATnn'nnATC Cuts at: 2609 7015 2609 Size: 4406 2615 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4298 4828 5210 5845 Size: 53 83 186 936 2765 530 382 635 Cuts at: 5845 275 Size: 1451 Fragments arranged by size: 2765 1451 936 635 530 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3549 3940 5454 5852 6126 6273 6684 3549 Size: 391 1514 398 274 147 411 3886 Fragments arranged by size: 3886 1514 411 398 391 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2045 2061 2075 2091 2417 Size: 30 165 30 642 16 14 16 326 Cuts at: 2417 2447 1178 Size: 30 5752 Fragments arranged by size: 5752 642 326 165 30 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 3429 1186 Size: 1255 988 4778 Fragments arranged by size: 4778 1255 988 BseYI C'CCAG_C Cuts at: 2276 3163 6224 6720 2276 Size: 887 3061 496 2577 Fragments arranged by size: 3061 2577 887 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2143 2643 4130 1163 Size: 573 407 500 1487 4054 Fragments arranged by size: 4054 1487 573 500 407 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4471 5232 6260 1540 Size: 275 186 2470 761 1028 2301 Fragments arranged by size: 2470 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5082 5167 6238 5082 Size: 85 1071 5865 Fragments arranged by size: 5865 1071 85 BsmI GAATG_Cn' Cuts at: 2581 3736 4532 4625 2581 Size: 1155 796 93 4977 Fragments arranged by size: 4977 1155 796 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2734 2734 Size: 7021 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3178 4340 5082 5167 6238 1391 Size: 399 1388 1162 742 85 1071 2174 Fragments arranged by size: 2174 1388 1162 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6684 6684 Size: 7021 BspHI T'CATG_A Cuts at: 4910 6640 4910 Size: 1730 5291 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4376 844 Size: 3532 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4908 1394 Size: 429 3085 3507 Fragments arranged by size: 3507 3085 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5516 5690 65 Size: 1183 4268 174 1396 Fragments arranged by size: 4268 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5665 5852 1700 Size: 110 3855 187 2869 Fragments arranged by size: 3855 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3626 96 Size: 3530 3491 BssHII G'CGCG_C Cuts at: 1925 2286 1925 Size: 361 6660 BssSI C'ACGA_G Cuts at: 5081 6093 5081 Size: 1012 6009 BstAPI GCAn_nnn'nTGC Cuts at: 794 2767 794 Size: 1973 5048 BstBI TT'CG_AA Cuts at: 3854 5825 3854 Size: 1971 5050 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7021 BstYI r'GATC_y Cuts at: 1972 2132 2564 2604 3270 3975 4351 4718 5103 Size: 160 432 40 666 705 376 367 385 Cuts at: 5103 5120 6572 6658 6670 7016 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 705 666 432 385 376 367 346 160 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 3169 3169 Size: 7021 BtgI C'CryG_G Cuts at: 513 1065 3051 513 Size: 552 1986 4483 Fragments arranged by size: 4483 1986 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6282 Fragments arranged by size: 6282 683 56 BtsI GCAGTG_nn' Cuts at: 2410 4537 5331 5351 2410 Size: 2127 794 20 4080 Fragments arranged by size: 4080 2127 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7021 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6986 DraI TTT'AAA Cuts at: 3361 3738 4325 3361 Size: 377 587 6057 Fragments arranged by size: 6057 587 377 DraIII CAC_nnn'GTG Cuts at: 3914 3914 Size: 7021 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6028 809 Size: 507 4712 1802 Fragments arranged by size: 4712 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4048 4468 5357 Size: 54 1425 50 275 18 2218 420 889 Cuts at: 5357 6986 8 Size: 1629 43 Fragments arranged by size: 2218 1629 1425 889 420 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4468 1537 Size: 275 2656 4090 Fragments arranged by size: 4090 2656 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2997 3136 4951 1353 Size: 1644 139 1815 3423 Fragments arranged by size: 3423 1815 1644 139 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5608 5982 6128 387 Size: 1064 138 4019 374 146 1280 Fragments arranged by size: 4019 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 3305 3520 4125 4161 4290 5084 Size: 393 456 1217 215 605 36 129 794 Cuts at: 5084 5681 6468 6736 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1217 794 787 605 597 456 393 268 215 129 36 EcoO109I rG'GnC_Cy Cuts at: 3061 3061 Size: 7021 EcoRI G'AATT_C Cuts at: 2677 4330 4708 2677 Size: 1653 378 4990 Fragments arranged by size: 4990 1653 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4059 4059 Size: 7021 FspI TGC'GCA Cuts at: 5527 5527 Size: 7021 HaeII r_GCGC'y Cuts at: 1678 6168 1678 Size: 4490 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2417 2449 3602 3634 3692 Size: 32 135 32 845 32 1153 32 58 Cuts at: 3692 3724 3890 3922 3983 4003 4015 4035 5662 Size: 32 166 32 61 20 12 20 1627 Cuts at: 5662 5694 5736 5768 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1627 1153 845 166 135 61 58 42 32 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3195 4365 4611 669 Size: 988 281 1257 1170 246 3079 Fragments arranged by size: 3079 1257 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7021 HpaI GTT'AAC Cuts at: 3195 4611 3195 Size: 1416 5605 KpnI G_GTAC'C Cuts at: 4346 4346 Size: 7021 MfeI C'AATT_G Cuts at: 4620 4620 Size: 7021 MluI A'CGCG_T Cuts at: 6897 6897 Size: 7021 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2110 2133 2658 2935 3395 3672 6135 6319 Size: 805 23 525 277 460 277 2463 184 Cuts at: 6319 1305 Size: 2007 Fragments arranged by size: 2463 2007 805 525 460 277 277 184 23 MscI TGG'CCA Cuts at: 10 64 1489 1832 4050 10 Size: 54 1425 343 2218 2981 Fragments arranged by size: 2981 2218 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2456 3531 3716 3943 4979 5338 5497 Size: 648 1290 1075 185 227 1036 359 159 Cuts at: 5497 518 Size: 2042 Fragments arranged by size: 2042 1290 1075 1036 648 359 227 185 159 MspA1I CmG'CkG Cuts at: 1606 2494 3091 4207 4437 5114 6262 6507 1606 Size: 888 597 1116 230 677 1148 245 2120 Fragments arranged by size: 2120 1148 1116 888 677 597 245 230 NcoI C'CATG_G Cuts at: 513 1065 3051 513 Size: 552 1986 4483 Fragments arranged by size: 4483 1986 552 NdeI CA'TA_TG Cuts at: 387 2212 387 Size: 1825 5196 NheI G'CTAG_C Cuts at: 1052 2464 1052 Size: 1412 5609 NotI GC'GGCC_GC Cuts at: 4468 4468 Size: 7021 NsiI A_TGCA'T Cuts at: 3538 3538 Size: 7021 NspI r_CATG'y Cuts at: 1132 1229 2875 3536 3540 4379 5924 1132 Size: 97 1646 661 4 839 1545 2229 Fragments arranged by size: 2229 1646 1545 839 661 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7021 PciI A'CATG_T Cuts at: 5920 5920 Size: 7021 PflMI CCAn_nnn'nTGG Cuts at: 1108 4207 1108 Size: 3099 3922 PmeI GTTT'AAAC Cuts at: 4325 4325 Size: 7021 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2370 2402 5090 5122 6603 6635 2370 Size: 32 2688 32 1481 32 2756 Fragments arranged by size: 2756 2688 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7021 PsiI TTA'TAA Cuts at: 4591 4591 Size: 7021 PstI C_TGCA'G Cuts at: 830 2591 4373 830 Size: 1761 1782 3478 Fragments arranged by size: 3478 1782 1761 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7021 PvuII CAG'CTG Cuts at: 2494 4207 2494 Size: 1713 5308 SalI G'TCGA_C Cuts at: 1936 4363 1936 Size: 2427 4594 SbfI CC_TGCA'GG Cuts at: 4373 4373 Size: 7021 ScaI AGT'ACT Cuts at: 3743 5269 6981 3743 Size: 1526 1712 3783 Fragments arranged by size: 3783 1712 1526 SexAI A'CCwGG_T Cuts at: 3787 3787 Size: 7021 SfcI C'TryA_G Cuts at: 826 1045 2587 4369 5504 6185 6376 826 Size: 219 1542 1782 1135 681 191 1471 Fragments arranged by size: 1782 1542 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7021 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7021 SmaI CCC'GGG Cuts at: 1872 4348 1872 Size: 2476 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2137 2884 5125 6026 6288 820 Size: 197 926 194 747 2241 901 262 1553 Fragments arranged by size: 2241 1553 926 901 747 262 197 194 SnaBI TAC'GTA Cuts at: 493 3466 4812 493 Size: 2973 1346 2702 Fragments arranged by size: 2973 2702 1346 SpeI A'CTAG_T Cuts at: 152 152 Size: 7021 SphI G_CATG'C Cuts at: 1132 2875 3536 4379 1132 Size: 1743 661 843 3774 Fragments arranged by size: 3774 1743 843 661 SspI AAT'ATT Cuts at: 5 52 3796 4945 5 Size: 47 3744 1149 2081 Fragments arranged by size: 3744 2081 1149 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7021 StyI C'CwwG_G Cuts at: 513 1065 2224 2240 3051 4460 513 Size: 552 1159 16 811 1409 3074 Fragments arranged by size: 3074 1409 1159 811 552 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3995 5048 5065 5218 5403 5848 325 Size: 1255 2415 1053 17 153 185 445 1498 Fragments arranged by size: 2415 1498 1255 1053 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3626 3741 5267 Size: 274 80 33 51 1443 1649 115 1526 Cuts at: 5267 6979 96 Size: 1712 138 Fragments arranged by size: 1712 1649 1526 1443 274 138 115 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6981 7013 6981 Size: 32 6989 Tth111I GACn'n_nGTC Cuts at: 1316 2629 1316 Size: 1313 5708 XbaI T'CTAG_A Cuts at: 4357 4357 Size: 7021 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7021 XmaI C'CCGG_G Cuts at: 1870 4346 1870 Size: 2476 4545 XmnI GAAnn'nnTTC Cuts at: 5150 5150 Size: 7021 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4829 276 Size: 53 83 186 936 3295 2468 Fragments arranged by size: 3295 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI