(Circular) (Six-base) MAPSORT of: pFN21AB5886.seq Check: 9266 from: 1 to: 6196 pFN21AB5886 6196 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2505 3076 2505 Size: 571 5625 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4006 278 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2379 3539 1937 Size: 442 1160 4594 Fragments arranged by size: 4594 1160 442 Acc65I G'GTAC_C Cuts at: 3517 3517 Size: 6196 AclI AA'CG_TT Cuts at: 1563 4323 4696 1563 Size: 2760 373 3063 Fragments arranged by size: 3063 2760 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2478 2834 4259 5643 5911 1632 Size: 846 356 1425 1384 268 1917 Fragments arranged by size: 1917 1425 1384 846 356 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5999 AflIII A'CryG_T Cuts at: 1203 5095 6072 1203 Size: 3892 977 1327 Fragments arranged by size: 3892 1327 977 AgeI A'CCGG_T Cuts at: 5027 5027 Size: 6196 AhdI GACnn_n'nnGTC Cuts at: 4925 4925 Size: 6196 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6196 AlwNI CAG_nnn'CTG Cuts at: 2836 2920 3939 5511 2836 Size: 84 1019 1572 3521 Fragments arranged by size: 3521 1572 1019 84 ApaLI G'TGCA_C Cuts at: 4253 5409 4253 Size: 1156 5040 ApoI r'AATT_y Cuts at: 1493 2447 2738 3505 3731 3883 5992 1493 Size: 954 291 767 226 152 2109 1697 Fragments arranged by size: 2109 1697 954 767 291 226 152 AseI AT'TA_AT Cuts at: 160 1032 4750 160 Size: 872 3718 1606 Fragments arranged by size: 3718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 AvaI C'yCGr_G Cuts at: 1870 1943 2054 2355 2625 3521 1870 Size: 73 111 301 270 896 4545 Fragments arranged by size: 4545 896 301 270 111 73 AvrII C'CTAG_G Cuts at: 2779 2779 Size: 6196 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3240 3273 3240 Size: 33 6163 BamHI G'GATC_C Cuts at: 2620 3526 3893 2620 Size: 906 367 4923 Fragments arranged by size: 4923 906 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3517 4972 618 Size: 325 217 627 1730 1455 1842 Fragments arranged by size: 1842 1730 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2122 2181 2376 2472 2540 3048 3075 3136 Size: 731 59 195 96 68 508 27 61 Cuts at: 3136 3321 3515 1391 Size: 185 194 4072 Fragments arranged by size: 4072 731 508 195 194 185 96 68 61 59 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2822 2875 928 Size: 971 923 53 4249 Fragments arranged by size: 4249 971 923 53 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3074 3108 4387 4421 1903 Size: 34 1137 34 1279 34 3678 Fragments arranged by size: 3678 1279 1137 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4088 5298 4088 Size: 1210 4986 BclI T'GATC_A Cuts at: 1548 1548 Size: 6196 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2488 2897 3443 Size: 107 122 71 631 605 816 409 546 Cuts at: 3443 4807 136 Size: 1364 1525 Fragments arranged by size: 1525 1364 816 631 605 546 409 122 107 71 BglII A'GATC_T Cuts at: 6191 6191 Size: 6196 BlpI GC'TnA_GC Cuts at: 2234 2763 3613 2234 Size: 529 850 4817 Fragments arranged by size: 4817 850 529 Bme1580I G_kGCm'C Cuts at: 1790 2073 4257 5413 1790 Size: 283 2184 1156 2573 Fragments arranged by size: 2573 2184 1156 283 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6196 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2245 2427 3046 Size: 416 53 83 399 24 828 182 619 Cuts at: 3046 3927 4885 442 Size: 881 958 1753 Fragments arranged by size: 1753 958 881 828 619 416 399 182 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6196 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2621 2792 2942 3416 3437 4856 1239 Size: 1382 171 150 474 21 1419 2579 Fragments arranged by size: 2579 1419 1382 474 171 150 21 Bpu10I CC'TnA_GC Cuts at: 3164 3164 Size: 6196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2279 2498 2627 3263 4321 5186 5484 2279 Size: 219 129 636 1058 865 298 2991 Fragments arranged by size: 2991 1058 865 636 298 219 129 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2687 4859 882 Size: 617 1188 2172 2219 Fragments arranged by size: 2219 2172 1188 617 BsaAI yAC'GTr Cuts at: 493 1204 2950 3987 493 Size: 711 1746 1037 2702 Fragments arranged by size: 2702 1746 1037 711 BsaBI GATnn'nnATC Cuts at: 6190 6190 Size: 6196 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4003 4385 5020 275 Size: 53 83 186 936 2470 382 635 1451 Fragments arranged by size: 2470 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2418 2724 4629 5027 5301 5448 5859 2418 Size: 306 1905 398 274 147 411 2755 Fragments arranged by size: 2755 1905 411 398 306 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 2621 2651 2990 Size: 30 165 30 678 30 510 30 339 Cuts at: 2990 3020 3212 3242 1178 Size: 30 192 30 4132 Fragments arranged by size: 4132 678 510 339 192 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 2134 3413 1186 Size: 852 96 1279 3969 Fragments arranged by size: 3969 1279 852 96 BseYI C'CCAG_C Cuts at: 2892 5399 5895 2892 Size: 2507 496 3193 Fragments arranged by size: 3193 2507 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2207 2639 2843 2933 2971 1163 Size: 573 471 432 204 90 38 4388 Fragments arranged by size: 4388 573 471 432 204 90 38 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3646 4407 5435 1540 Size: 275 186 1645 761 1028 2301 Fragments arranged by size: 2301 1645 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 2354 2748 2826 2949 3240 3279 3321 4257 Size: 173 394 78 123 291 39 42 936 Cuts at: 4257 4342 5413 2181 Size: 85 1071 2964 Fragments arranged by size: 2964 1071 936 394 291 173 123 85 78 42 39 BsmI GAATG_Cn' Cuts at: 3707 3800 3707 Size: 93 6103 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 2003 Size: 6196 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2122 2181 2354 2376 2472 2540 Size: 399 283 49 59 173 22 96 68 Cuts at: 2540 2748 2826 2949 3048 3075 3136 3240 3279 Size: 208 78 123 99 27 61 104 39 Cuts at: 3279 3321 3515 4257 4342 5413 1391 Size: 42 194 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 283 208 194 173 123 104 99 96 85 78 68 61 59 49 42 39 27 22 BspEI T'CCGG_A Cuts at: 2418 5859 2418 Size: 3441 2755 BspHI T'CATG_A Cuts at: 4085 5815 4085 Size: 1730 4466 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2505 2993 3076 3551 844 Size: 1661 488 83 475 3489 Fragments arranged by size: 3489 1661 488 475 83 BsrBI CCG'CTC Cuts at: 1394 1823 2133 3130 4083 1394 Size: 429 310 997 953 3507 Fragments arranged by size: 3507 997 953 429 310 BsrDI GCAATG_nn' Cuts at: 65 1248 4691 4865 65 Size: 1183 3443 174 1396 Fragments arranged by size: 3443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2209 3414 4840 5027 1700 Size: 110 399 1205 1426 187 2869 Fragments arranged by size: 2869 1426 1205 399 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6196 BssSI C'ACGA_G Cuts at: 2225 4256 5268 2225 Size: 2031 1012 3153 Fragments arranged by size: 3153 2031 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2230 2706 794 Size: 1436 476 4284 Fragments arranged by size: 4284 1436 476 BstBI TT'CG_AA Cuts at: 5000 5000 Size: 6196 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6196 BstYI r'GATC_y Cuts at: 1972 2620 2775 3063 3526 3893 4278 4295 5747 Size: 648 155 288 463 367 385 17 1452 Cuts at: 5747 5833 5845 6191 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 648 463 385 367 346 288 155 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2556 513 Size: 552 939 552 4153 Fragments arranged by size: 4153 939 552 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5457 Fragments arranged by size: 5457 683 56 BtsI GCAGTG_nn' Cuts at: 3712 4506 4526 3712 Size: 794 20 5382 Fragments arranged by size: 5382 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6196 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6161 DraI TTT'AAA Cuts at: 3500 3500 Size: 6196 DraIII CAC_nnn'GTG Cuts at: 3193 3193 Size: 6196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5203 809 Size: 507 3887 1802 Fragments arranged by size: 3887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2286 2489 2559 Size: 54 1425 50 275 18 456 203 70 Cuts at: 2559 2975 3474 3643 4532 6161 8 Size: 416 499 169 889 1629 43 Fragments arranged by size: 1629 1425 889 499 456 416 275 203 169 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3643 1537 Size: 275 1831 4090 Fragments arranged by size: 4090 1831 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2454 3018 4126 1353 Size: 1101 564 1108 3423 Fragments arranged by size: 3423 1108 1101 564 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4783 5157 5303 387 Size: 1064 138 3194 374 146 1280 Fragments arranged by size: 3194 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2179 3319 2179 Size: 1140 5056 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2478 2621 2792 2834 2942 3416 3437 Size: 393 846 143 171 42 108 474 21 Cuts at: 3437 4259 4856 5643 5911 1239 Size: 822 597 787 268 1524 Fragments arranged by size: 1524 846 822 787 597 474 393 268 171 143 108 42 21 EcoNI CCTnn'n_nnAGG Cuts at: 2924 3173 2924 Size: 249 5947 EcoO109I rG'GnC_Cy Cuts at: 3213 3426 3445 3213 Size: 213 19 5964 Fragments arranged by size: 5964 213 19 EcoRI G'AATT_C Cuts at: 2738 3505 3883 2738 Size: 767 378 5051 Fragments arranged by size: 5051 767 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3047 3047 Size: 6196 FspI TGC'GCA Cuts at: 4702 4702 Size: 6196 HaeII r_GCGC'y Cuts at: 1678 5343 1678 Size: 3665 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 4837 4869 4911 Size: 32 135 32 509 32 2724 32 42 Cuts at: 4911 4943 1373 Size: 32 2626 Fragments arranged by size: 2724 2626 509 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3540 3786 669 Size: 988 281 1602 246 3079 Fragments arranged by size: 3079 1602 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6196 HpaI GTT'AAC Cuts at: 3786 3786 Size: 6196 KpnI G_GTAC'C Cuts at: 3521 3521 Size: 6196 MfeI C'AATT_G Cuts at: 3795 3795 Size: 6196 MluI A'CGCG_T Cuts at: 6072 6072 Size: 6196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5310 5494 1305 Size: 4005 184 2007 Fragments arranged by size: 4005 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2491 2561 2977 10 Size: 54 1425 343 659 70 416 3229 Fragments arranged by size: 3229 1425 659 416 343 70 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 2751 3293 4154 4513 4672 518 Size: 648 1230 355 542 861 359 159 2042 Fragments arranged by size: 2042 1230 861 648 542 359 355 159 MspA1I CmG'CkG Cuts at: 1606 2188 2503 2896 3457 3612 4289 5437 5682 Size: 582 315 393 561 155 677 1148 245 Cuts at: 5682 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 582 561 393 315 245 155 NaeI GCC'GGC Cuts at: 2211 3416 2211 Size: 1205 4991 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4705 Fragments arranged by size: 4705 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6196 NgoMIV G'CCGG_C Cuts at: 2209 3414 2209 Size: 1205 4991 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6196 NotI GC'GGCC_GC Cuts at: 3643 3643 Size: 6196 NspI r_CATG'y Cuts at: 1132 1229 3554 5099 1132 Size: 97 2325 1545 2229 Fragments arranged by size: 2325 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3430 1348 Size: 2082 4114 PciI A'CATG_T Cuts at: 5095 5095 Size: 6196 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6196 PfoI T'CCnGG_A Cuts at: 2598 2919 3066 2598 Size: 321 147 5728 Fragments arranged by size: 5728 321 147 PmeI GTTT'AAAC Cuts at: 3500 3500 Size: 6196 PmlI CAC'GTG Cuts at: 2950 2950 Size: 6196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4265 4297 5778 5810 4265 Size: 32 1481 32 4651 Fragments arranged by size: 4651 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3213 3426 3213 Size: 213 5983 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6196 PsiI TTA'TAA Cuts at: 3766 3766 Size: 6196 PstI C_TGCA'G Cuts at: 830 2502 3548 830 Size: 1672 1046 3478 Fragments arranged by size: 3478 1672 1046 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6196 PvuII CAG'CTG Cuts at: 2503 2896 2503 Size: 393 5803 SacI G_AGCT'C Cuts at: 2181 3321 2181 Size: 1140 5056 SalI G'TCGA_C Cuts at: 1936 3538 1936 Size: 1602 4594 SanDI GG'GwC_CC Cuts at: 3213 3426 3213 Size: 213 5983 SbfI CC_TGCA'GG Cuts at: 3548 3548 Size: 6196 ScaI AGT'ACT Cuts at: 4444 6156 4444 Size: 1712 4484 SfcI C'TryA_G Cuts at: 826 1045 2498 3419 3544 4679 5360 5551 826 Size: 219 1453 921 125 1135 681 191 1471 Fragments arranged by size: 1471 1453 1135 921 681 219 191 125 SfiI GGCCn_nnn'nGGCC Cuts at: 3443 3443 Size: 6196 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6196 SmaI CCC'GGG Cuts at: 1872 2056 2357 2627 3523 1872 Size: 184 301 270 896 4545 Fragments arranged by size: 4545 896 301 270 184 SmlI C'TyrA_G Cuts at: 820 1017 1943 2294 2477 2642 3278 4300 5201 Size: 197 926 351 183 165 636 1022 901 Cuts at: 5201 5463 820 Size: 262 1553 Fragments arranged by size: 1553 1022 926 901 636 351 262 197 183 165 SnaBI TAC'GTA Cuts at: 493 3987 493 Size: 3494 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6196 SphI G_CATG'C Cuts at: 1132 3554 1132 Size: 2422 3774 SspI AAT'ATT Cuts at: 5 52 4120 5 Size: 47 4068 2081 Fragments arranged by size: 4068 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6196 StyI C'CwwG_G Cuts at: 513 1065 2004 2779 3231 3438 3635 513 Size: 552 939 775 452 207 197 3074 Fragments arranged by size: 3074 939 775 552 452 207 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3005 4223 4240 4393 4578 5023 325 Size: 1255 1425 1218 17 153 185 445 1498 Fragments arranged by size: 1498 1425 1255 1218 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4442 6154 96 Size: 274 80 33 51 1443 2465 1712 138 Fragments arranged by size: 2465 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6156 6188 6156 Size: 32 6164 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6196 XbaI T'CTAG_A Cuts at: 3532 3532 Size: 6196 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2569 2569 Size: 6196 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6196 XmaI C'CCGG_G Cuts at: 1870 2054 2355 2625 3521 1870 Size: 184 301 270 896 4545 Fragments arranged by size: 4545 896 301 270 184 XmnI GAAnn'nnTTC Cuts at: 2414 3027 4325 2414 Size: 613 1298 4285 Fragments arranged by size: 4285 1298 613 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4004 276 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI