(Circular) (Six-base) MAPSORT of: pFN21AE0133.seq Check: 1017 from: 1 to: 5488 pFN21AE0133 5488 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3298 278 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2831 1937 Size: 894 4594 Acc65I G'GTAC_C Cuts at: 2809 2809 Size: 5488 AclI AA'CG_TT Cuts at: 1563 3615 3988 1563 Size: 2052 373 3063 Fragments arranged by size: 3063 2052 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2279 3551 4935 5203 1632 Size: 647 1272 1384 268 1917 Fragments arranged by size: 1917 1384 1272 647 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5291 AflIII A'CryG_T Cuts at: 1203 2265 2783 4387 5364 1203 Size: 1062 518 1604 977 1327 Fragments arranged by size: 1604 1327 1062 977 518 AgeI A'CCGG_T Cuts at: 4319 4319 Size: 5488 AhdI GACnn_n'nnGTC Cuts at: 4217 4217 Size: 5488 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5488 AlwNI CAG_nnn'CTG Cuts at: 2308 2539 3231 4803 2308 Size: 231 692 1572 2993 Fragments arranged by size: 2993 1572 692 231 ApaLI G'TGCA_C Cuts at: 2033 3545 4701 2033 Size: 1512 1156 2820 Fragments arranged by size: 2820 1512 1156 ApoI r'AATT_y Cuts at: 1493 2797 3023 3175 5284 1493 Size: 1304 226 152 2109 1697 Fragments arranged by size: 2109 1697 1304 226 152 AseI AT'TA_AT Cuts at: 160 1032 4042 160 Size: 872 3010 1606 Fragments arranged by size: 3010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 AvaI C'yCGr_G Cuts at: 1870 1943 2813 1870 Size: 73 870 4545 Fragments arranged by size: 4545 870 73 BamHI G'GATC_C Cuts at: 2818 3185 2818 Size: 367 5121 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2131 2328 2391 2809 4264 Size: 325 217 627 344 197 63 418 1455 Cuts at: 4264 618 Size: 1842 Fragments arranged by size: 1842 1455 627 418 344 325 217 197 63 BanII G_rGCy'C Cuts at: 1391 2807 1391 Size: 1416 4072 BbeI G_GCGC'C Cuts at: 2135 2332 2135 Size: 197 5291 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2786 928 Size: 971 887 3630 Fragments arranged by size: 3630 971 887 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3679 3713 1903 Size: 34 1742 34 3678 Fragments arranged by size: 3678 1742 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3380 4590 3380 Size: 1210 4278 BclI T'GATC_A Cuts at: 1548 1548 Size: 5488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2159 2676 4099 Size: 107 122 71 631 605 487 517 1423 Cuts at: 4099 136 Size: 1525 Fragments arranged by size: 1525 1423 631 605 517 487 122 107 71 BglII A'GATC_T Cuts at: 5483 5483 Size: 5488 BlpI GC'TnA_GC Cuts at: 2905 2905 Size: 5488 Bme1580I G_kGCm'C Cuts at: 1790 2037 3549 4705 1790 Size: 247 1512 1156 2573 Fragments arranged by size: 2573 1512 1156 247 BmgBI CAC'GTC Cuts at: 1318 2266 2704 1318 Size: 948 438 4102 Fragments arranged by size: 4102 948 438 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2513 3219 4177 Size: 416 53 83 399 24 1096 706 958 Cuts at: 4177 442 Size: 1753 Fragments arranged by size: 1753 1096 958 706 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5488 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2464 2464 Size: 5488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2693 4148 1239 Size: 1454 1455 2579 Fragments arranged by size: 2579 1455 1454 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2459 2609 3613 4478 4776 2459 Size: 150 1004 865 298 3171 Fragments arranged by size: 3171 1004 865 298 150 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4151 882 Size: 617 2652 2219 Fragments arranged by size: 2652 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2038 2515 3279 493 Size: 711 834 477 764 2702 Fragments arranged by size: 2702 834 764 711 477 BsaBI GATnn'nnATC Cuts at: 5482 5482 Size: 5488 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2132 2329 2656 3295 Size: 53 83 186 936 599 197 327 639 Cuts at: 3295 3677 4312 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 639 635 599 382 327 197 186 83 53 BsaWI w'CCGG_w Cuts at: 3921 4319 4593 4740 5151 3921 Size: 398 274 147 411 4258 Fragments arranged by size: 4258 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5263 Fragments arranged by size: 5263 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5488 BseYI C'CCAG_C Cuts at: 2203 2242 4691 5187 2203 Size: 39 2449 496 2504 Fragments arranged by size: 2504 2449 496 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 2321 1163 Size: 573 468 117 4330 Fragments arranged by size: 4330 573 468 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2938 3699 4727 1540 Size: 275 186 937 761 1028 2301 Fragments arranged by size: 2301 1028 937 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2037 2121 2512 2601 2709 3549 3634 4705 2037 Size: 84 391 89 108 840 85 1071 2820 Fragments arranged by size: 2820 1071 840 391 108 89 85 84 BsmI GAATG_Cn' Cuts at: 2999 3092 2999 Size: 93 5395 BsmBI CGTCTCn'nnnn_ Cuts at: 2452 2663 2452 Size: 211 5277 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2121 2512 2601 2709 2807 3549 Size: 399 247 84 391 89 108 98 742 Cuts at: 3549 3634 4705 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 391 247 108 98 89 85 84 BspEI T'CCGG_A Cuts at: 5151 5151 Size: 5488 BspHI T'CATG_A Cuts at: 3377 5107 3377 Size: 1730 3758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2843 844 Size: 1999 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3375 1394 Size: 429 1552 3507 Fragments arranged by size: 3507 1552 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3983 4157 65 Size: 1183 2735 174 1396 Fragments arranged by size: 2735 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 2141 4132 4319 1700 Size: 110 238 93 1991 187 2869 Fragments arranged by size: 2869 1991 238 187 110 93 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5488 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5488 BssSI C'ACGA_G Cuts at: 2180 3548 4560 2180 Size: 1368 1012 3108 Fragments arranged by size: 3108 1368 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2308 2507 2539 794 Size: 1514 199 32 3743 Fragments arranged by size: 3743 1514 199 32 BstBI TT'CG_AA Cuts at: 4292 4292 Size: 5488 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5488 BstXI CCAn_nnnn'nTGG Cuts at: 2771 2771 Size: 5488 BstYI r'GATC_y Cuts at: 1972 2818 3185 3570 3587 5039 5125 5137 5483 Size: 846 367 385 17 1452 86 12 346 Cuts at: 5483 1972 Size: 1977 Fragments arranged by size: 1977 1452 846 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2043 2064 2136 2277 2337 2382 2407 Size: 552 978 21 72 141 60 45 25 Cuts at: 2407 513 Size: 3594 Fragments arranged by size: 3594 978 552 141 72 60 45 25 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2249 487 Size: 683 56 1023 3726 Fragments arranged by size: 3726 1023 683 56 BtsI GCAGTG_nn' Cuts at: 2309 2330 2360 3004 3798 3818 2309 Size: 21 30 644 794 20 3979 Fragments arranged by size: 3979 794 644 30 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5453 DraI TTT'AAA Cuts at: 2792 2792 Size: 5488 DraIII CAC_nnn'GTG Cuts at: 2507 2518 2507 Size: 11 5477 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4495 809 Size: 507 3179 1802 Fragments arranged by size: 3179 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2046 2139 Size: 54 1425 50 275 18 210 6 93 Cuts at: 2139 2160 2454 2520 2935 3824 5453 8 Size: 21 294 66 415 889 1629 43 Fragments arranged by size: 1629 1425 889 415 294 275 210 93 66 54 50 43 21 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2935 1537 Size: 275 1123 4090 Fragments arranged by size: 4090 1123 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2666 3418 1353 Size: 1313 752 3423 Fragments arranged by size: 3423 1313 752 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4075 4449 4595 387 Size: 1064 138 2486 374 146 1280 Fragments arranged by size: 2486 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2693 3551 4148 4935 5203 1239 Size: 393 647 414 858 597 787 268 1524 Fragments arranged by size: 1524 858 787 647 597 414 393 268 EcoO109I rG'GnC_Cy Cuts at: 2246 2246 Size: 5488 EcoRI G'AATT_C Cuts at: 2797 3175 2797 Size: 378 5110 FseI GG_CCGG'CC Cuts at: 2145 2145 Size: 5488 FspI TGC'GCA Cuts at: 2543 3994 2543 Size: 1451 4037 HaeII r_GCGC'y Cuts at: 1678 2135 2332 2540 4635 1678 Size: 457 197 208 2095 2531 Fragments arranged by size: 2531 2095 457 208 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4129 4161 4203 4235 1373 Size: 32 135 32 2557 32 42 32 2626 Fragments arranged by size: 2626 2557 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2832 3078 669 Size: 988 281 894 246 3079 Fragments arranged by size: 3079 988 894 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5488 HpaI GTT'AAC Cuts at: 3078 3078 Size: 5488 KasI G'GCGC_C Cuts at: 2131 2328 2131 Size: 197 5291 KpnI G_GTAC'C Cuts at: 2813 2813 Size: 5488 MfeI C'AATT_G Cuts at: 3087 3087 Size: 5488 MluI A'CGCG_T Cuts at: 2783 5364 2783 Size: 2581 2907 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4602 4786 1305 Size: 3297 184 2007 Fragments arranged by size: 3297 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2162 2456 10 Size: 54 1425 343 330 294 3042 Fragments arranged by size: 3042 1425 343 330 294 54 MslI CAynn'nnrTG Cuts at: 518 1166 3446 3805 3964 518 Size: 648 2280 359 159 2042 Fragments arranged by size: 2280 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2339 2409 2525 2904 3581 4729 4974 1606 Size: 733 70 116 379 677 1148 245 2120 Fragments arranged by size: 2120 1148 733 677 379 245 116 70 NaeI GCC'GGC Cuts at: 2143 2143 Size: 5488 NarI GG'CG_CC Cuts at: 2132 2329 2132 Size: 197 5291 NcoI C'CATG_G Cuts at: 513 1065 2277 513 Size: 552 1212 3724 Fragments arranged by size: 3724 1212 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5488 NgoMIV G'CCGG_C Cuts at: 2141 2141 Size: 5488 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5488 NotI GC'GGCC_GC Cuts at: 2935 2935 Size: 5488 NspI r_CATG'y Cuts at: 1132 1229 2846 4391 1132 Size: 97 1617 1545 2229 Fragments arranged by size: 2229 1617 1545 97 PasI CC'CwG_GG Cuts at: 1348 2485 1348 Size: 1137 4351 PciI A'CATG_T Cuts at: 4387 4387 Size: 5488 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5488 PmeI GTTT'AAAC Cuts at: 2792 2792 Size: 5488 PmlI CAC'GTG Cuts at: 2038 2515 2038 Size: 477 5011 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3557 3589 5070 5102 3557 Size: 32 1481 32 3943 Fragments arranged by size: 3943 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2246 2246 Size: 5488 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5488 PsiI TTA'TAA Cuts at: 3058 3058 Size: 5488 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2177 2209 2177 Size: 32 5456 PstI C_TGCA'G Cuts at: 830 2840 830 Size: 2010 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5488 SacII CC_GC'GG Cuts at: 2340 2410 2340 Size: 70 5418 SalI G'TCGA_C Cuts at: 1936 2830 1936 Size: 894 4594 SanDI GG'GwC_CC Cuts at: 2246 2246 Size: 5488 SbfI CC_TGCA'GG Cuts at: 2840 2840 Size: 5488 ScaI AGT'ACT Cuts at: 3736 5448 3736 Size: 1712 3776 SfcI C'TryA_G Cuts at: 826 1045 2836 3971 4652 4843 826 Size: 219 1791 1135 681 191 1471 Fragments arranged by size: 1791 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2133 2330 2133 Size: 197 5291 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5488 SmaI CCC'GGG Cuts at: 1872 2815 1872 Size: 943 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2438 2624 3592 4493 4755 820 Size: 197 926 495 186 968 901 262 1553 Fragments arranged by size: 1553 968 926 901 495 262 197 186 SnaBI TAC'GTA Cuts at: 493 3279 493 Size: 2786 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5488 SphI G_CATG'C Cuts at: 1132 2846 1132 Size: 1714 3774 SspI AAT'ATT Cuts at: 5 52 3412 5 Size: 47 3360 2081 Fragments arranged by size: 3360 2081 47 StuI AGG'CCT Cuts at: 1517 2027 1517 Size: 510 4978 StyI C'CwwG_G Cuts at: 513 1065 2277 2691 2927 513 Size: 552 1212 414 236 3074 Fragments arranged by size: 3074 1212 552 414 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2495 2561 2769 3515 3532 3685 3870 Size: 1255 915 66 208 746 17 153 185 Cuts at: 3870 4315 325 Size: 445 1498 Fragments arranged by size: 1498 1255 915 746 445 208 185 153 66 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3734 5446 96 Size: 274 80 33 51 1443 1757 1712 138 Fragments arranged by size: 1757 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2672 2704 2770 2802 5448 5480 2672 Size: 32 66 32 2646 32 2680 Fragments arranged by size: 2680 2646 66 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5488 XbaI T'CTAG_A Cuts at: 2824 2824 Size: 5488 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2555 2555 Size: 5488 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5488 XmaI C'CCGG_G Cuts at: 1870 2813 1870 Size: 943 4545 XmnI GAAnn'nnTTC Cuts at: 3617 3617 Size: 5488 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3296 276 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PspOMI PspXI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI