Restriction map for
pFN21AE0133
(Linear) (Six-base) MAPSORT of: KIEE0133.seq Check: 7394 from: 1 to: 783
KIEE0133.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 783
Size: 274 509
AflIII A'CryG_T
Cuts at: 0 260 778 783
Size: 260 518 5
Fragments arranged by size:
518 260 5
AlwNI CAG_nnn'CTG
Cuts at: 0 303 534 783
Size: 303 231 249
Fragments arranged by size:
303 249 231
ApaLI G'TGCA_C
Cuts at: 0 28 783
Size: 28 755
BanI G'GyrC_C
Cuts at: 0 126 323 386 783
Size: 126 197 63 397
Fragments arranged by size:
397 197 126 63
BbeI G_GCGC'C
Cuts at: 0 130 327 783
Size: 130 197 456
Fragments arranged by size:
456 197 130
BbsI GAAGACnn'nnnn_
Cuts at: 0 781 783
Size: 781 2
BglI GCCn_nnn'nGGC
Cuts at: 0 154 671 783
Size: 154 517 112
Fragments arranged by size:
517 154 112
Bme1580I G_kGCm'C
Cuts at: 0 32 783
Size: 32 751
BmgBI CAC'GTC
Cuts at: 0 261 699 783
Size: 261 438 84
Fragments arranged by size:
438 261 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 508 783
Size: 508 275
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 459 783
Size: 459 324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 783
Size: 688 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 604 783
Size: 454 150 179
Fragments arranged by size:
454 179 150
BsaAI yAC'GTr
Cuts at: 0 33 510 783
Size: 33 477 273
Fragments arranged by size:
477 273 33
BsaHI Gr'CG_yC
Cuts at: 0 127 324 651 783
Size: 127 197 327 132
Fragments arranged by size:
327 197 132 127
BseYI C'CCAG_C
Cuts at: 0 198 237 783
Size: 198 39 546
Fragments arranged by size:
546 198 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 316 783
Size: 199 117 467
Fragments arranged by size:
467 199 117
BsiHKAI G_wGCw'C
Cuts at: 0 32 116 507 596 704 783
Size: 32 84 391 89 108 79
Fragments arranged by size:
391 108 89 84 79 32
BsmBI CGTCTCn'nnnn_
Cuts at: 0 447 658 783
Size: 447 211 125
Fragments arranged by size:
447 211 125
Bsp1286I G_dGCh'C
Cuts at: 0 32 116 507 596 704 783
Size: 32 84 391 89 108 79
Fragments arranged by size:
391 108 89 84 79 32
BsrFI r'CCGG_y
Cuts at: 0 43 136 783
Size: 43 93 647
Fragments arranged by size:
647 93 43
BssSI C'ACGA_G
Cuts at: 0 175 783
Size: 175 608
BstAPI GCAn_nnn'nTGC
Cuts at: 0 303 502 534 783
Size: 303 199 32 249
Fragments arranged by size:
303 249 199 32
BstXI CCAn_nnnn'nTGG
Cuts at: 0 766 783
Size: 766 17
BtgI C'CryG_G
Cuts at: 0 38 59 131 272 332 377 402 783
Size: 38 21 72 141 60 45 25 381
Fragments arranged by size:
381 141 72 60 45 38 25 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 244 783
Size: 244 539
BtsI GCAGTG_nn'
Cuts at: 0 304 325 355 783
Size: 304 21 30 428
Fragments arranged by size:
428 304 30 21
DraIII CAC_nnn'GTG
Cuts at: 0 502 513 783
Size: 502 11 270
Fragments arranged by size:
502 270 11
EaeI y'GGCC_r
Cuts at: 0 35 41 134 155 449 515 783
Size: 35 6 93 21 294 66 268
Fragments arranged by size:
294 268 93 66 35 21 6
EarI CTCTTCn'nnn_
Cuts at: 0 661 783
Size: 661 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 688 783
Size: 274 414 95
Fragments arranged by size:
414 274 95
EcoO109I rG'GnC_Cy
Cuts at: 0 241 783
Size: 241 542
FseI GG_CCGG'CC
Cuts at: 0 140 783
Size: 140 643
FspI TGC'GCA
Cuts at: 0 538 783
Size: 538 245
HaeII r_GCGC'y
Cuts at: 0 130 327 535 783
Size: 130 197 208 248
Fragments arranged by size:
248 208 197 130
KasI G'GCGC_C
Cuts at: 0 126 323 783
Size: 126 197 460
Fragments arranged by size:
460 197 126
MluI A'CGCG_T
Cuts at: 0 778 783
Size: 778 5
MscI TGG'CCA
Cuts at: 0 157 451 783
Size: 157 294 332
Fragments arranged by size:
332 294 157
MspA1I CmG'CkG
Cuts at: 0 334 404 520 783
Size: 334 70 116 263
Fragments arranged by size:
334 263 116 70
NaeI GCC'GGC
Cuts at: 0 138 783
Size: 138 645
NarI GG'CG_CC
Cuts at: 0 127 324 783
Size: 127 197 459
Fragments arranged by size:
459 197 127
NcoI C'CATG_G
Cuts at: 0 272 783
Size: 272 511
NgoMIV G'CCGG_C
Cuts at: 0 136 783
Size: 136 647
PasI CC'CwG_GG
Cuts at: 0 480 783
Size: 480 303
PmlI CAC'GTG
Cuts at: 0 33 510 783
Size: 33 477 273
Fragments arranged by size:
477 273 33
PpuMI rG'GwC_Cy
Cuts at: 0 241 783
Size: 241 542
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 172 204 783
Size: 172 32 579
Fragments arranged by size:
579 172 32
SacII CC_GC'GG
Cuts at: 0 335 405 783
Size: 335 70 378
Fragments arranged by size:
378 335 70
SanDI GG'GwC_CC
Cuts at: 0 241 783
Size: 241 542
SfoI GGC'GCC
Cuts at: 0 128 325 783
Size: 128 197 458
Fragments arranged by size:
458 197 128
SmlI C'TyrA_G
Cuts at: 0 433 619 783
Size: 433 186 164
Fragments arranged by size:
433 186 164
StuI AGG'CCT
Cuts at: 0 22 783
Size: 22 761
StyI C'CwwG_G
Cuts at: 0 272 686 783
Size: 272 414 97
Fragments arranged by size:
414 272 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 490 556 764 783
Size: 490 66 208 19
Fragments arranged by size:
490 208 66 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 667 699 783
Size: 667 32 84
Fragments arranged by size:
667 84 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 550 783
Size: 550 233
Enzymes that do cut:
AcuI AflIII AlwNI ApaLI BanI BbeI BbsI BglI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI BsaHI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrFI BssSI BstAPI
BstXI BtgI BtgZI BtsI DraIII EaeI EarI Eco57MI
EcoO109I FseI FspI HaeII KasI MluI MscI MspA1I
NaeI NarI NcoI NgoMIV PasI PmlI PpuMI PsrI
SacII SanDI SfoI SmlI StuI StyI TaqII TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmtI Bpu10I BsaI
BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BstBI BstEII
BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MmeI MslI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmaI XmnI ZraI