(Circular) (Six-base) MAPSORT of: pFN21AA1032.seq Check: 3130 from: 1 to: 9814 pFN21AA1032 9814 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6847 6847 Size: 9814 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7624 278 Size: 53 83 186 936 6088 2468 Fragments arranged by size: 6088 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7157 1937 Size: 5220 4594 Acc65I G'GTAC_C Cuts at: 7135 7135 Size: 9814 AclI AA'CG_TT Cuts at: 1563 7941 8314 1563 Size: 6378 373 3063 Fragments arranged by size: 6378 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2427 3039 5277 5855 5865 5882 6137 6149 Size: 795 612 2238 578 10 17 255 12 Cuts at: 6149 7877 9261 9529 1632 Size: 1728 1384 268 1917 Fragments arranged by size: 2238 1917 1728 1384 795 612 578 268 255 17 12 10 AfeI AGC'GCT Cuts at: 4799 5254 4799 Size: 455 9359 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9617 AflIII A'CryG_T Cuts at: 1203 2067 4344 4752 8713 9690 1203 Size: 864 2277 408 3961 977 1327 Fragments arranged by size: 3961 2277 1327 977 864 408 AgeI A'CCGG_T Cuts at: 8645 8645 Size: 9814 AhdI GACnn_n'nnGTC Cuts at: 3334 4015 5146 8543 3334 Size: 681 1131 3397 4605 Fragments arranged by size: 4605 3397 1131 681 AleI CACnn'nnGTG Cuts at: 1166 2072 2225 4410 4751 6429 1166 Size: 906 153 2185 341 1678 4551 Fragments arranged by size: 4551 2185 1678 906 341 153 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2466 3840 4238 1184 Size: 1282 1374 398 6760 Fragments arranged by size: 6760 1374 1282 398 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2138 2170 5795 5827 2138 Size: 32 3625 32 6125 Fragments arranged by size: 6125 3625 32 32 AlwNI CAG_nnn'CTG Cuts at: 3796 5233 5461 6012 6629 7557 9129 3796 Size: 1437 228 551 617 928 1572 4481 Fragments arranged by size: 4481 1572 1437 928 617 551 228 ApaI G_GGCC'C Cuts at: 2282 5124 6732 2282 Size: 2842 1608 5364 Fragments arranged by size: 5364 2842 1608 ApaLI G'TGCA_C Cuts at: 3652 7871 9027 3652 Size: 4219 1156 4439 Fragments arranged by size: 4439 4219 1156 ApoI r'AATT_y Cuts at: 1493 2060 4180 5194 7123 7349 7501 9610 1493 Size: 567 2120 1014 1929 226 152 2109 1697 Fragments arranged by size: 2120 2109 1929 1697 1014 567 226 152 AseI AT'TA_AT Cuts at: 160 1032 8368 160 Size: 872 7336 1606 Fragments arranged by size: 7336 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9814 AvaI C'yCGr_G Cuts at: 1870 1943 2709 3399 3920 4974 5484 5621 6839 Size: 73 766 690 521 1054 510 137 1218 Cuts at: 6839 7139 1870 Size: 300 4545 Fragments arranged by size: 4545 1218 1054 766 690 521 510 300 137 73 BamHI G'GATC_C Cuts at: 2241 4088 7144 7511 2241 Size: 1847 3056 367 4544 Fragments arranged by size: 4544 3056 1847 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2339 3055 3175 3235 Size: 325 217 627 261 291 716 120 60 Cuts at: 3235 4391 4681 5501 7097 7135 8590 618 Size: 1156 290 820 1596 38 1455 1842 Fragments arranged by size: 1842 1596 1455 1156 820 716 627 325 291 290 261 217 120 60 38 BanII G_rGCy'C Cuts at: 1391 2282 2708 2873 3111 3839 3936 4970 5124 Size: 891 426 165 238 728 97 1034 154 Cuts at: 5124 5904 6470 6732 6790 7133 1391 Size: 780 566 262 58 343 4072 Fragments arranged by size: 4072 1034 891 780 728 566 426 343 262 238 165 154 97 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3096 3626 4130 5193 5572 6113 6455 Size: 971 1197 530 504 1063 379 541 342 Cuts at: 6455 6582 928 Size: 127 4160 Fragments arranged by size: 4160 1197 1063 971 541 530 504 379 342 127 BbvCI CC'TCA_GC Cuts at: 2768 2768 Size: 9814 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2092 2126 4687 4721 8005 8039 1903 Size: 34 155 34 2561 34 3284 34 3678 Fragments arranged by size: 3678 3284 2561 155 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7706 8916 7706 Size: 1210 8604 BclI T'GATC_A Cuts at: 1548 1548 Size: 9814 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2868 3173 3206 Size: 107 122 71 631 605 1196 305 33 Cuts at: 3206 3450 8425 136 Size: 244 4975 1525 Fragments arranged by size: 4975 1525 1196 631 605 305 244 122 107 71 33 BglII A'GATC_T Cuts at: 2331 3924 3945 9809 2331 Size: 1593 21 5864 2336 Fragments arranged by size: 5864 2336 1593 21 BlpI GC'TnA_GC Cuts at: 2810 2831 3105 3246 3264 4328 5903 6737 6821 Size: 21 274 141 18 1064 1575 834 84 Cuts at: 6821 7231 2810 Size: 410 5393 Fragments arranged by size: 5393 1575 1064 834 410 274 141 84 21 18 Bme1580I G_kGCm'C Cuts at: 1790 2053 2234 2282 3656 4676 5124 5506 5652 Size: 263 181 48 1374 1020 448 382 146 Cuts at: 5652 5760 5990 6732 7875 9031 1790 Size: 108 230 742 1143 1156 2573 Fragments arranged by size: 2573 1374 1156 1143 1020 742 448 382 263 230 181 146 108 48 BmgBI CAC'GTC Cuts at: 1318 4017 4345 4501 1318 Size: 2699 328 156 6631 Fragments arranged by size: 6631 2699 328 156 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2434 3164 6639 Size: 416 53 83 399 24 1017 730 3475 Cuts at: 6639 7545 8503 442 Size: 906 958 1753 Fragments arranged by size: 3475 1753 1017 958 906 730 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9814 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2767 3853 2767 Size: 1086 8728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2304 2459 2996 3008 3020 3056 3559 4877 Size: 1065 155 537 12 12 36 503 1318 Cuts at: 4877 4946 5528 5678 5822 6479 8474 1239 Size: 69 582 150 144 657 1995 2579 Fragments arranged by size: 2579 1995 1318 1065 657 582 537 503 155 150 144 69 36 12 12 Bpu10I CC'TnA_GC Cuts at: 2768 5972 2768 Size: 3204 6610 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3869 6238 6314 6428 6659 7049 7939 8804 9102 Size: 2369 76 114 231 390 890 865 298 Cuts at: 9102 3869 Size: 4581 Fragments arranged by size: 4581 2369 890 865 390 298 231 114 76 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2213 3461 8477 882 Size: 617 714 1248 5016 2219 Fragments arranged by size: 5016 2219 1248 714 617 BsaAI yAC'GTr Cuts at: 493 1204 2070 2074 7605 493 Size: 711 866 4 5531 2702 Fragments arranged by size: 5531 2702 866 711 4 BsaBI GATnn'nnATC Cuts at: 4040 9808 4040 Size: 5768 4046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3659 4614 7621 8003 Size: 53 83 186 936 2126 955 3007 382 Cuts at: 8003 8638 275 Size: 635 1451 Fragments arranged by size: 3007 2126 1451 955 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3858 4857 8247 8645 8919 9066 9477 3858 Size: 999 3390 398 274 147 411 4195 Fragments arranged by size: 4195 3390 999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2711 2741 2885 2915 4190 Size: 30 165 30 1308 30 144 30 1275 Cuts at: 4190 4220 4944 4974 5696 5711 5726 5741 5795 Size: 30 724 30 722 15 15 15 54 Cuts at: 5795 5825 7064 7094 1178 Size: 30 1239 30 3898 Fragments arranged by size: 3898 1308 1275 1239 724 722 165 144 54 30 30 30 30 30 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2426 2759 2993 3017 3020 3023 3041 Size: 1102 138 333 234 24 3 3 18 Cuts at: 3041 3044 3053 3056 3059 3062 3065 3296 3608 Size: 3 9 3 3 3 3 231 312 Cuts at: 3608 4190 4445 4648 5318 5489 5681 7103 1186 Size: 582 255 203 670 171 192 1422 3897 Fragments arranged by size: 3897 1422 1102 670 582 333 312 255 234 231 203 192 171 138 24 18 9 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2132 2136 2513 2547 2952 3081 4870 5123 6310 Size: 4 377 34 405 129 1789 253 1187 Cuts at: 6310 6732 6783 6936 9017 9513 2132 Size: 422 51 153 2081 496 2433 Fragments arranged by size: 2433 2081 1789 1187 496 422 405 377 253 153 129 51 34 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2108 2601 3739 4523 5480 1163 Size: 573 372 493 1138 784 957 5497 Fragments arranged by size: 5497 1138 957 784 573 493 372 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4213 4768 6704 6973 7264 8025 Size: 275 186 2212 555 1936 269 291 761 Cuts at: 8025 9053 1540 Size: 1028 2301 Fragments arranged by size: 2301 2212 1936 1028 761 555 291 275 269 186 BsiHKAI G_wGCw'C Cuts at: 2105 2859 3656 3839 3885 3936 4700 5310 5691 Size: 754 797 183 46 51 764 610 381 Cuts at: 5691 5904 7875 7960 9031 2105 Size: 213 1971 85 1071 2888 Fragments arranged by size: 2888 1971 1071 797 764 754 610 381 213 183 85 51 46 BsiWI C'GTAC_G Cuts at: 2070 2070 Size: 9814 BsmI GAATG_Cn' Cuts at: 5763 7325 7418 5763 Size: 1562 93 8159 Fragments arranged by size: 8159 1562 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2105 2234 2282 2708 2859 2873 Size: 399 263 52 129 48 426 151 14 Cuts at: 2873 3111 3656 3839 3885 3936 4676 4700 4970 Size: 238 545 183 46 51 740 24 270 Cuts at: 4970 5124 5310 5506 5652 5691 5760 5904 5990 Size: 154 186 196 146 39 69 144 86 Cuts at: 5990 6470 6732 6790 7133 7875 7960 9031 1391 Size: 480 262 58 343 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 740 545 480 426 399 343 270 263 262 238 196 186 183 154 151 146 144 129 86 85 69 58 52 51 48 46 39 24 14 BspEI T'CCGG_A Cuts at: 9477 9477 Size: 9814 BspHI T'CATG_A Cuts at: 4692 5769 7703 9433 4692 Size: 1077 1934 1730 5073 Fragments arranged by size: 5073 1934 1730 1077 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3804 6072 6483 6531 6847 7169 844 Size: 2960 2268 411 48 316 322 3489 Fragments arranged by size: 3489 2960 2268 411 322 316 48 BsrBI CCG'CTC Cuts at: 1394 1823 2890 6967 7701 1394 Size: 429 1067 4077 734 3507 Fragments arranged by size: 4077 3507 1067 734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6001 8309 8483 65 Size: 1183 4753 2308 174 1396 Fragments arranged by size: 4753 2308 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8458 8645 1700 Size: 110 6648 187 2869 Fragments arranged by size: 6648 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5058 6541 96 Size: 4962 1483 3369 Fragments arranged by size: 4962 3369 1483 BssHII G'CGCG_C Cuts at: 1925 6884 1925 Size: 4959 4855 BssSI C'ACGA_G Cuts at: 3509 3649 3779 7874 8886 3509 Size: 140 130 4095 1012 4437 Fragments arranged by size: 4437 4095 1012 140 130 BstAPI GCAn_nnn'nTGC Cuts at: 794 2523 2863 3748 3753 3762 4003 6484 794 Size: 1729 340 885 5 9 241 2481 4124 Fragments arranged by size: 4124 2481 1729 885 340 241 9 5 BstBI TT'CG_AA Cuts at: 3672 5814 8618 3672 Size: 2142 2804 4868 Fragments arranged by size: 4868 2804 2142 BstEII G'GTnAC_C Cuts at: 1184 3955 1184 Size: 2771 7043 BstXI CCAn_nnnn'nTGG Cuts at: 2437 3532 4987 6310 6431 2437 Size: 1095 1455 1323 121 5820 Fragments arranged by size: 5820 1455 1323 1095 121 BstYI r'GATC_y Cuts at: 1972 2241 2331 3252 3900 3924 3945 4088 4524 Size: 269 90 921 648 24 21 143 436 Cuts at: 4524 7144 7511 7896 7913 9365 9451 9463 9809 Size: 2620 367 385 17 1452 86 12 346 Cuts at: 9809 1972 Size: 1977 Fragments arranged by size: 2620 1977 1452 921 648 436 385 367 346 269 143 90 86 24 21 17 12 Bsu36I CC'TnA_GG Cuts at: 3258 3258 Size: 9814 BtgI C'CryG_G Cuts at: 513 1065 2775 4047 5298 5568 5820 6159 513 Size: 552 1710 1272 1251 270 252 339 4168 Fragments arranged by size: 4168 1710 1272 1251 552 339 270 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2095 3718 487 Size: 683 56 869 1623 6583 Fragments arranged by size: 6583 1623 869 683 56 BtsI GCAGTG_nn' Cuts at: 2589 2617 6383 7330 8124 8144 2589 Size: 28 3766 947 794 20 4259 Fragments arranged by size: 4259 3766 947 794 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9814 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9779 DraI TTT'AAA Cuts at: 7118 7118 Size: 9814 DraIII CAC_nnn'GTG Cuts at: 2238 3762 4492 2238 Size: 1524 730 7560 Fragments arranged by size: 7560 1524 730 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2309 4103 8821 809 Size: 507 993 1794 4718 1802 Fragments arranged by size: 4718 1802 1794 993 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3198 3522 3684 Size: 54 1425 50 275 18 1368 324 162 Cuts at: 3684 5085 6701 6909 6970 7044 7261 8150 9779 Size: 1401 1616 208 61 74 217 889 1629 Cuts at: 9779 8 Size: 43 Fragments arranged by size: 1629 1616 1425 1401 1368 889 324 275 217 208 162 74 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6701 6970 7261 1537 Size: 275 4889 269 291 4090 Fragments arranged by size: 4889 4090 291 275 269 EarI CTCTTCn'nnn_ Cuts at: 1353 2403 2803 2964 3827 5937 6458 7744 1353 Size: 1050 400 161 863 2110 521 1286 3423 Fragments arranged by size: 3423 2110 1286 1050 863 521 400 161 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 3866 4018 7072 8401 8775 Size: 1064 138 660 1617 152 3054 1329 374 Cuts at: 8775 8921 387 Size: 146 1280 Fragments arranged by size: 3054 1617 1329 1280 1064 660 374 152 146 138 EcoICRI GAG'CTC Cuts at: 3837 3934 5902 3837 Size: 97 1968 7749 Fragments arranged by size: 7749 1968 97 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2304 2427 2459 2996 3008 3020 3039 Size: 393 672 123 32 537 12 12 19 Cuts at: 3039 3056 3559 4877 4946 5277 5528 5678 5822 Size: 17 503 1318 69 331 251 150 144 Cuts at: 5822 5855 5865 5882 6137 6149 6479 7877 8474 Size: 33 10 17 255 12 330 1398 597 Cuts at: 8474 9261 9529 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1398 1318 787 672 597 537 503 393 331 330 268 255 251 150 144 123 69 33 32 19 17 17 12 12 12 10 EcoNI CCTnn'n_nnAGG Cuts at: 3002 5975 3002 Size: 2973 6841 EcoO109I rG'GnC_Cy Cuts at: 2278 2279 2347 2983 3166 3187 3793 4897 5120 Size: 1 68 636 183 21 606 1104 223 Cuts at: 5120 6729 6836 2278 Size: 1609 107 5256 Fragments arranged by size: 5256 1609 1104 636 606 223 183 107 68 21 1 EcoRI G'AATT_C Cuts at: 5194 7123 7501 5194 Size: 1929 378 7507 Fragments arranged by size: 7507 1929 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5030 6469 5030 Size: 1439 8375 FspI TGC'GCA Cuts at: 8320 8320 Size: 9814 HaeII r_GCGC'y Cuts at: 1678 4801 5256 5665 6828 6951 8961 1678 Size: 3123 455 409 1163 123 2010 2531 Fragments arranged by size: 3123 2531 2010 1163 455 409 123 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2152 2184 2885 2917 3320 Size: 32 135 32 580 32 701 32 403 Cuts at: 3320 3352 4001 4033 4238 4270 4889 4921 5132 Size: 32 649 32 205 32 619 32 211 Cuts at: 5132 5164 5254 5286 5465 5497 5567 5599 5696 Size: 32 90 32 179 32 70 32 97 Cuts at: 5696 5711 5728 5743 6464 6496 7064 7096 8455 Size: 15 17 15 721 32 568 32 1359 Cuts at: 8455 8487 8529 8561 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1359 721 701 649 619 580 568 403 211 205 179 135 97 90 70 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 6718 7158 7404 669 Size: 988 281 4780 440 246 3079 Fragments arranged by size: 4780 3079 988 440 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9814 HpaI GTT'AAC Cuts at: 7404 7404 Size: 9814 KpnI G_GTAC'C Cuts at: 7139 7139 Size: 9814 MfeI C'AATT_G Cuts at: 7413 7413 Size: 9814 MluI A'CGCG_T Cuts at: 9690 9690 Size: 9814 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2002 2898 4233 4788 4806 7050 8928 9112 Size: 697 896 1335 555 18 2244 1878 184 Cuts at: 9112 1305 Size: 2007 Fragments arranged by size: 2244 2007 1878 1335 896 697 555 184 18 MscI TGG'CCA Cuts at: 10 64 1489 1832 3200 3524 7046 10 Size: 54 1425 343 1368 324 3522 2778 Fragments arranged by size: 3522 2778 1425 1368 343 324 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2114 2225 2565 3875 3878 4046 Size: 648 906 42 111 340 1310 3 168 Cuts at: 4046 4220 4316 4410 4412 4751 5768 6429 6983 Size: 174 96 94 2 339 1017 661 554 Cuts at: 6983 7772 8131 8290 518 Size: 789 359 159 2042 Fragments arranged by size: 2042 1310 1017 906 789 661 648 554 359 340 339 174 168 159 111 96 94 42 3 2 MspA1I CmG'CkG Cuts at: 1606 2136 2830 3143 3245 3388 3820 4332 4822 Size: 530 694 313 102 143 432 512 490 Cuts at: 4822 5800 6319 6922 7230 7907 9055 9300 1606 Size: 978 519 603 308 677 1148 245 2120 Fragments arranged by size: 2120 1148 978 694 677 603 530 519 512 490 432 313 308 245 143 102 NcoI C'CATG_G Cuts at: 513 1065 5298 5820 6159 513 Size: 552 4233 522 339 4168 Fragments arranged by size: 4233 4168 552 522 339 NdeI CA'TA_TG Cuts at: 387 387 Size: 9814 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9814 NotI GC'GGCC_GC Cuts at: 7261 7261 Size: 9814 NruI TCG'CGA Cuts at: 3737 3737 Size: 9814 NspI r_CATG'y Cuts at: 1132 1229 3478 3751 5552 7172 8717 1132 Size: 97 2249 273 1801 1620 1545 2229 Fragments arranged by size: 2249 2229 1801 1620 1545 273 97 PasI CC'CwG_GG Cuts at: 1348 2701 3149 3446 4060 4676 6559 1348 Size: 1353 448 297 614 616 1883 4603 Fragments arranged by size: 4603 1883 1353 616 614 448 297 PciI A'CATG_T Cuts at: 8713 8713 Size: 9814 PflMI CCAn_nnn'nTGG Cuts at: 1108 5461 6565 6629 1108 Size: 4353 1104 64 4293 Fragments arranged by size: 4353 4293 1104 64 PfoI T'CCnGG_A Cuts at: 2370 2997 3542 3903 3939 4812 4893 6456 6622 Size: 627 545 361 36 873 81 1563 166 Cuts at: 6622 2370 Size: 5562 Fragments arranged by size: 5562 1563 873 627 545 361 166 81 36 PmeI GTTT'AAAC Cuts at: 7118 7118 Size: 9814 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4466 4498 5795 5827 7883 7915 9396 9428 4466 Size: 32 1297 32 2056 32 1481 32 4852 Fragments arranged by size: 4852 2056 1481 1297 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2347 2983 3793 4897 6836 2347 Size: 636 810 1104 1939 5325 Fragments arranged by size: 5325 1939 1104 810 636 PshAI GACnn'nnGTC Cuts at: 1613 6834 1613 Size: 5221 4593 PsiI TTA'TAA Cuts at: 7384 7384 Size: 9814 PspOMI G'GGCC_C Cuts at: 2278 5120 6728 2278 Size: 2842 1608 5364 Fragments arranged by size: 5364 2842 1608 PspXI vC'TCGA_Gb Cuts at: 5621 5621 Size: 9814 PstI C_TGCA'G Cuts at: 830 2868 3244 3393 3819 6017 6858 6921 7166 Size: 2038 376 149 426 2198 841 63 245 Cuts at: 7166 830 Size: 3478 Fragments arranged by size: 3478 2198 2038 841 426 376 245 149 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9814 PvuII CAG'CTG Cuts at: 2136 2830 3143 3245 3388 4822 5800 6319 6922 Size: 694 313 102 143 1434 978 519 603 Cuts at: 6922 2136 Size: 5028 Fragments arranged by size: 5028 1434 978 694 603 519 313 143 102 SacI G_AGCT'C Cuts at: 3839 3936 5904 3839 Size: 97 1968 7749 Fragments arranged by size: 7749 1968 97 SalI G'TCGA_C Cuts at: 1936 7156 1936 Size: 5220 4594 SanDI GG'GwC_CC Cuts at: 6836 6836 Size: 9814 SapI GCTCTTCn'nnn_ Cuts at: 2964 3827 6458 2964 Size: 863 2631 6320 Fragments arranged by size: 6320 2631 863 SbfI CC_TGCA'GG Cuts at: 2868 7166 2868 Size: 4298 5516 ScaI AGT'ACT Cuts at: 8062 9774 8062 Size: 1712 8102 SfcI C'TryA_G Cuts at: 826 1045 2719 2864 3240 3389 3815 6013 6854 Size: 219 1674 145 376 149 426 2198 841 Cuts at: 6854 6917 7162 8297 8978 9169 826 Size: 63 245 1135 681 191 1471 Fragments arranged by size: 2198 1674 1471 1135 841 681 426 376 245 219 191 149 145 63 SfiI GGCCn_nnn'nGGCC Cuts at: 3206 3206 Size: 9814 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9814 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9814 SmaI CCC'GGG Cuts at: 1872 2711 3401 5486 7141 1872 Size: 839 690 2085 1655 4545 Fragments arranged by size: 4545 2085 1655 839 690 SmlI C'TyrA_G Cuts at: 820 1017 1943 3884 5621 6217 6329 6443 6674 Size: 197 926 1941 1737 596 112 114 231 Cuts at: 6674 7064 7918 8819 9081 820 Size: 390 854 901 262 1553 Fragments arranged by size: 1941 1737 1553 926 901 854 596 390 262 231 197 114 112 SnaBI TAC'GTA Cuts at: 493 7605 493 Size: 7112 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9814 SphI G_CATG'C Cuts at: 1132 3478 3751 5552 7172 1132 Size: 2346 273 1801 1620 3774 Fragments arranged by size: 3774 2346 1801 1620 273 SspI AAT'ATT Cuts at: 5 52 2474 2681 6419 7738 5 Size: 47 2422 207 3738 1319 2081 Fragments arranged by size: 3738 2422 2081 1319 207 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9814 StyI C'CwwG_G Cuts at: 513 1065 2343 3201 3273 3345 3567 4536 5298 Size: 552 1278 858 72 72 222 969 762 Cuts at: 5298 5715 5739 5820 6159 6303 7047 7253 513 Size: 417 24 81 339 144 744 206 3074 Fragments arranged by size: 3074 1278 969 858 762 744 552 417 339 222 206 144 81 72 72 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2914 3193 3286 4511 5951 6557 6575 Size: 1255 1334 279 93 1225 1440 606 18 Cuts at: 6575 7841 7858 8011 8196 8641 325 Size: 1266 17 153 185 445 1498 Fragments arranged by size: 1498 1440 1334 1266 1255 1225 606 445 279 185 153 93 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4458 5031 5058 Size: 274 80 33 51 1443 2481 573 27 Cuts at: 5058 6541 6795 8060 9772 96 Size: 1483 254 1265 1712 138 Fragments arranged by size: 2481 1712 1483 1443 1265 573 274 254 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2172 2204 4190 4222 9774 9806 2172 Size: 32 1986 32 5552 32 2180 Fragments arranged by size: 5552 2180 1986 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9814 XbaI T'CTAG_A Cuts at: 7150 7150 Size: 9814 XhoI C'TCGA_G Cuts at: 1943 5621 1943 Size: 3678 6136 XmaI C'CCGG_G Cuts at: 1870 2709 3399 5484 7139 1870 Size: 839 690 2085 1655 4545 Fragments arranged by size: 4545 2085 1655 839 690 XmnI GAAnn'nnTTC Cuts at: 4481 4865 5810 6452 7943 4481 Size: 384 945 642 1491 6352 Fragments arranged by size: 6352 1491 945 642 384 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7622 276 Size: 53 83 186 936 6088 2468 Fragments arranged by size: 6088 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PsrI RsrII SacII SexAI SfoI SrfI SwaI XcmI