(Circular) (Six-base) MAPSORT of: pFN21AB5816.seq Check: 6191 from: 1 to: 7330 pFN21AB5816 7330 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2104 2364 5140 278 Size: 53 83 186 936 568 260 2776 2468 Fragments arranged by size: 2776 2468 936 568 260 186 83 53 AccI GT'mk_AC Cuts at: 1937 4673 1937 Size: 2736 4594 Acc65I G'GTAC_C Cuts at: 3330 4651 3330 Size: 1321 6009 AclI AA'CG_TT Cuts at: 1563 5457 5830 1563 Size: 3894 373 3063 Fragments arranged by size: 3894 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5393 6777 7045 1632 Size: 3761 1384 268 1917 Fragments arranged by size: 3761 1917 1384 268 AfeI AGC'GCT Cuts at: 2198 2516 3168 2198 Size: 318 652 6360 Fragments arranged by size: 6360 652 318 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7133 AflIII A'CryG_T Cuts at: 1203 6229 7206 1203 Size: 5026 977 1327 Fragments arranged by size: 5026 1327 977 AgeI A'CCGG_T Cuts at: 6161 6161 Size: 7330 AhdI GACnn_n'nnGTC Cuts at: 2669 6059 2669 Size: 3390 3940 AleI CACnn'nnGTG Cuts at: 1166 3075 1166 Size: 1909 5421 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7330 AlwNI CAG_nnn'CTG Cuts at: 3113 4123 4384 5073 6645 3113 Size: 1010 261 689 1572 3798 Fragments arranged by size: 3798 1572 1010 689 261 ApaI G_GGCC'C Cuts at: 2324 2653 3945 2324 Size: 329 1292 5709 Fragments arranged by size: 5709 1292 329 ApaLI G'TGCA_C Cuts at: 5387 6543 5387 Size: 1156 6174 ApoI r'AATT_y Cuts at: 1493 4163 4639 4865 5017 7126 1493 Size: 2670 476 226 152 2109 1697 Fragments arranged by size: 2670 2109 1697 476 226 152 AseI AT'TA_AT Cuts at: 160 1032 5884 160 Size: 872 4852 1606 Fragments arranged by size: 4852 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 AvaI C'yCGr_G Cuts at: 1870 1943 2643 3300 4655 1870 Size: 73 700 657 1355 4545 Fragments arranged by size: 4545 1355 700 657 73 AvrII C'CTAG_G Cuts at: 2887 2887 Size: 7330 BamHI G'GATC_C Cuts at: 4660 5027 4660 Size: 367 6963 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3330 4651 6106 618 Size: 325 217 627 1543 1321 1455 1842 Fragments arranged by size: 1842 1543 1455 1321 627 325 217 BanII G_rGCy'C Cuts at: 1391 2324 2653 2853 3366 3482 3945 4649 1391 Size: 933 329 200 513 116 463 704 4072 Fragments arranged by size: 4072 933 704 513 463 329 200 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2459 2665 2940 3027 3998 928 Size: 971 560 206 275 87 971 4260 Fragments arranged by size: 4260 971 971 560 275 206 87 BbvCI CC'TCA_GC Cuts at: 2340 3141 2340 Size: 801 6529 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5521 5555 1903 Size: 34 3584 34 3678 Fragments arranged by size: 3678 3584 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5222 6432 5222 Size: 1210 6120 BclI T'GATC_A Cuts at: 1548 1548 Size: 7330 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2648 5941 136 Size: 107 122 71 631 605 976 3293 1525 Fragments arranged by size: 3293 1525 976 631 605 122 107 71 BglII A'GATC_T Cuts at: 3432 7325 3432 Size: 3893 3437 BlpI GC'TnA_GC Cuts at: 3476 4272 4747 3476 Size: 796 475 6059 Fragments arranged by size: 6059 796 475 Bme1580I G_kGCm'C Cuts at: 1790 2324 2653 3945 5391 6547 1790 Size: 534 329 1292 1446 1156 2573 Fragments arranged by size: 2573 1446 1292 1156 534 329 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7330 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2263 4133 5061 Size: 416 53 83 399 24 846 1870 928 Cuts at: 5061 6019 442 Size: 958 1753 Fragments arranged by size: 1870 1753 958 928 846 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7330 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2218 2284 3331 4057 2218 Size: 66 1047 726 5491 Fragments arranged by size: 5491 1047 726 66 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2828 2898 3251 4001 5990 1239 Size: 1589 70 353 750 1989 2579 Fragments arranged by size: 2579 1989 1589 750 353 70 Bpu10I CC'TnA_GC Cuts at: 2340 3141 2340 Size: 801 6529 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3155 5455 6320 6618 3155 Size: 2300 865 298 3867 Fragments arranged by size: 3867 2300 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4563 5993 882 Size: 617 3064 1430 2219 Fragments arranged by size: 3064 2219 1430 617 BsaAI yAC'GTr Cuts at: 493 1204 5121 493 Size: 711 3917 2702 Fragments arranged by size: 3917 2702 711 BsaBI GATnn'nnATC Cuts at: 7324 7324 Size: 7330 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2101 2361 3219 3632 Size: 53 83 186 936 568 260 858 413 Cuts at: 3632 5137 5519 6154 275 Size: 1505 382 635 1451 Fragments arranged by size: 1505 1451 936 858 635 568 413 382 260 186 83 53 BsaWI w'CCGG_w Cuts at: 2181 2541 3063 5763 6161 6435 6582 6993 2181 Size: 360 522 2700 398 274 147 411 2518 Fragments arranged by size: 2700 2518 522 411 398 360 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7105 Fragments arranged by size: 7105 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2260 2462 2600 2831 2834 2837 2840 3158 Size: 1074 202 138 231 3 3 3 318 Cuts at: 3158 3161 3170 3391 3731 3734 3737 3741 3773 Size: 3 9 221 340 3 3 4 32 Cuts at: 3773 3827 4016 1186 Size: 54 189 4500 Fragments arranged by size: 4500 1074 340 318 231 221 202 189 138 54 32 9 4 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2306 2404 2852 3366 3396 3534 3730 4279 4305 Size: 98 448 514 30 138 196 549 26 Cuts at: 4305 6533 7029 2306 Size: 2228 496 2607 Fragments arranged by size: 2607 2228 549 514 496 448 196 138 98 30 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6757 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2032 2077 3248 3412 4780 5541 Size: 275 186 31 45 1171 164 1368 761 Cuts at: 5541 6569 1540 Size: 1028 2301 Fragments arranged by size: 2301 1368 1171 1028 761 275 186 164 45 31 BsiHKAI G_wGCw'C Cuts at: 2265 5391 5476 6547 2265 Size: 3126 85 1071 3048 Fragments arranged by size: 3126 3048 1071 85 BsmI GAATG_Cn' Cuts at: 3859 4841 4934 3859 Size: 982 93 6255 Fragments arranged by size: 6255 982 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2492 3464 2492 Size: 972 6358 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2265 2324 2653 2853 3366 3482 3945 Size: 399 475 59 329 200 513 116 463 Cuts at: 3945 4649 5391 5476 6547 1391 Size: 704 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 704 513 475 463 399 329 200 116 85 59 BspEI T'CCGG_A Cuts at: 2541 6993 2541 Size: 4452 2878 BspHI T'CATG_A Cuts at: 5219 6949 5219 Size: 1730 5600 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3269 3453 3855 4685 844 Size: 2425 184 402 830 3489 Fragments arranged by size: 3489 2425 830 402 184 BsrBI CCG'CTC Cuts at: 1394 1823 3132 3802 5217 1394 Size: 429 1309 670 1415 3507 Fragments arranged by size: 3507 1415 1309 670 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2412 5825 5999 65 Size: 1183 1164 3413 174 1396 Fragments arranged by size: 3413 1396 1183 1164 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5974 6161 1700 Size: 110 4164 187 2869 Fragments arranged by size: 4164 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7330 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7330 BssSI C'ACGA_G Cuts at: 3646 5390 6402 3646 Size: 1744 1012 4574 Fragments arranged by size: 4574 1744 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2554 3550 794 Size: 1760 996 4574 Fragments arranged by size: 4574 1760 996 BstBI TT'CG_AA Cuts at: 6134 6134 Size: 7330 BstEII G'GTnAC_C Cuts at: 1184 3400 1184 Size: 2216 5114 BstXI CCAn_nnnn'nTGG Cuts at: 4313 4313 Size: 7330 BstYI r'GATC_y Cuts at: 1972 3432 4660 5027 5412 5429 6881 6967 6979 Size: 1460 1228 367 385 17 1452 86 12 Cuts at: 6979 7325 1972 Size: 346 1977 Fragments arranged by size: 1977 1460 1452 1228 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3756 3768 3756 Size: 12 7318 BtgI C'CryG_G Cuts at: 513 1065 2004 2137 3222 3242 513 Size: 552 939 133 1085 20 4601 Fragments arranged by size: 4601 1085 939 552 133 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3268 487 Size: 683 56 2042 4549 Fragments arranged by size: 4549 2042 683 56 BtsI GCAGTG_nn' Cuts at: 3421 4846 5640 5660 3421 Size: 1425 794 20 5091 Fragments arranged by size: 5091 1425 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7330 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3901 3936 529 Size: 35 3337 35 3923 Fragments arranged by size: 3923 3337 35 35 DraI TTT'AAA Cuts at: 4634 4634 Size: 7330 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6337 809 Size: 507 5021 1802 Fragments arranged by size: 5021 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2140 3133 3245 Size: 54 1425 50 275 18 310 993 112 Cuts at: 3245 3573 4777 5666 7295 8 Size: 328 1204 889 1629 43 Fragments arranged by size: 1629 1425 1204 993 889 328 310 275 112 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3245 4777 1537 Size: 275 1433 1532 4090 Fragments arranged by size: 4090 1532 1433 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2050 3003 3275 4044 5260 1353 Size: 697 953 272 769 1216 3423 Fragments arranged by size: 3423 1216 953 769 697 272 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4430 5917 6291 6437 387 Size: 1064 138 2841 1487 374 146 1280 Fragments arranged by size: 2841 1487 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2828 2898 3251 4001 5393 5990 6777 Size: 393 1196 70 353 750 1392 597 787 Cuts at: 6777 7045 1239 Size: 268 1524 Fragments arranged by size: 1524 1392 1196 787 750 597 393 353 268 70 EcoNI CCTnn'n_nnAGG Cuts at: 2810 3698 3771 2810 Size: 888 73 6369 Fragments arranged by size: 6369 888 73 EcoO109I rG'GnC_Cy Cuts at: 2320 2649 2650 3496 3941 4172 4381 4463 4597 Size: 329 1 846 445 231 209 82 134 Cuts at: 4597 2320 Size: 5053 Fragments arranged by size: 5053 846 445 329 231 209 134 82 1 EcoRI G'AATT_C Cuts at: 4639 5017 4639 Size: 378 6952 FspI TGC'GCA Cuts at: 3278 3554 3851 4187 5836 3278 Size: 276 297 336 1649 4772 Fragments arranged by size: 4772 1649 336 297 276 HaeII r_GCGC'y Cuts at: 1678 2152 2200 2518 3170 3356 3714 3750 4279 Size: 474 48 318 652 186 358 36 529 Cuts at: 4279 6477 1678 Size: 2198 2531 Fragments arranged by size: 2531 2198 652 529 474 358 318 186 48 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2655 2687 3101 3107 3133 Size: 32 135 32 1083 32 414 6 26 Cuts at: 3133 3139 3898 3930 5971 6003 6045 6077 1373 Size: 6 759 32 2041 32 42 32 2626 Fragments arranged by size: 2626 2041 1083 759 414 135 42 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3421 4674 4920 669 Size: 988 281 1483 1253 246 3079 Fragments arranged by size: 3079 1483 1253 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7330 HpaI GTT'AAC Cuts at: 3421 4920 3421 Size: 1499 5831 KpnI G_GTAC'C Cuts at: 3334 4655 3334 Size: 1321 6009 MfeI C'AATT_G Cuts at: 4929 4929 Size: 7330 MluI A'CGCG_T Cuts at: 7206 7206 Size: 7330 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2967 3099 3419 4134 6444 6628 1305 Size: 1662 132 320 715 2310 184 2007 Fragments arranged by size: 2310 2007 1662 715 320 184 132 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5508 Fragments arranged by size: 5508 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2580 2613 3075 5288 5647 5806 518 Size: 648 1414 33 462 2213 359 159 2042 Fragments arranged by size: 2213 2042 1414 648 462 359 159 33 MspA1I CmG'CkG Cuts at: 1606 3224 3244 3250 3741 4746 5423 6571 6816 Size: 1618 20 6 491 1005 677 1148 245 Cuts at: 6816 1606 Size: 2120 Fragments arranged by size: 2120 1618 1148 1005 677 491 245 20 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5839 Fragments arranged by size: 5839 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7330 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7330 NotI GC'GGCC_GC Cuts at: 3245 4777 3245 Size: 1532 5798 NspI r_CATG'y Cuts at: 1132 1229 4211 4688 6233 1132 Size: 97 2982 477 1545 2229 Fragments arranged by size: 2982 2229 1545 477 97 PasI CC'CwG_GG Cuts at: 1348 2346 4378 1348 Size: 998 2032 4300 Fragments arranged by size: 4300 2032 998 PciI A'CATG_T Cuts at: 6229 6229 Size: 7330 PflMI CCAn_nnn'nTGG Cuts at: 1108 2330 3592 4063 1108 Size: 1222 1262 471 4375 Fragments arranged by size: 4375 1262 1222 471 PfoI T'CCnGG_A Cuts at: 2715 2805 3462 2715 Size: 90 657 6583 Fragments arranged by size: 6583 657 90 PmeI GTTT'AAAC Cuts at: 4634 4634 Size: 7330 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5399 5431 6912 6944 5399 Size: 32 1481 32 5785 Fragments arranged by size: 5785 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3496 4172 4597 3496 Size: 676 425 6229 Fragments arranged by size: 6229 676 425 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7330 PsiI TTA'TAA Cuts at: 4900 4900 Size: 7330 PspOMI G'GGCC_C Cuts at: 2320 2649 3941 2320 Size: 329 1292 5709 Fragments arranged by size: 5709 1292 329 PstI C_TGCA'G Cuts at: 830 3450 4450 4682 830 Size: 2620 1000 232 3478 Fragments arranged by size: 3478 2620 1000 232 PvuI CG_AT'CG Cuts at: 2001 2032 2001 Size: 31 7299 SacII CC_GC'GG Cuts at: 3225 3245 3225 Size: 20 7310 SalI G'TCGA_C Cuts at: 1936 4672 1936 Size: 2736 4594 SbfI CC_TGCA'GG Cuts at: 4682 4682 Size: 7330 ScaI AGT'ACT Cuts at: 5578 7290 5578 Size: 1712 5618 SfcI C'TryA_G Cuts at: 826 1045 3446 4446 4678 5813 6494 6685 826 Size: 219 2401 1000 232 1135 681 191 1471 Fragments arranged by size: 2401 1471 1135 1000 681 232 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2648 2648 Size: 7330 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7330 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7330 SmaI CCC'GGG Cuts at: 1872 2645 3302 4657 1872 Size: 773 657 1355 4545 Fragments arranged by size: 4545 1355 773 657 SmlI C'TyrA_G Cuts at: 820 1017 1943 3170 5434 6335 6597 820 Size: 197 926 1227 2264 901 262 1553 Fragments arranged by size: 2264 1553 1227 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5121 493 Size: 4628 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7330 SphI G_CATG'C Cuts at: 1132 4211 4688 1132 Size: 3079 477 3774 Fragments arranged by size: 3774 3079 477 SspI AAT'ATT Cuts at: 5 52 5254 5 Size: 47 5202 2081 Fragments arranged by size: 5202 2081 47 StuI AGG'CCT Cuts at: 1517 2886 1517 Size: 1369 5961 StyI C'CwwG_G Cuts at: 513 1065 2004 2887 3189 3213 3864 4574 4769 Size: 552 939 883 302 24 651 710 195 Cuts at: 4769 513 Size: 3074 Fragments arranged by size: 3074 939 883 710 651 552 302 195 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2420 2586 2625 3388 3548 3967 Size: 1255 417 423 166 39 763 160 419 Cuts at: 3967 5357 5374 5527 5712 6157 325 Size: 1390 17 153 185 445 1498 Fragments arranged by size: 1498 1390 1255 763 445 423 419 417 185 166 160 153 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5576 7288 96 Size: 274 80 33 51 1443 3599 1712 138 Fragments arranged by size: 3599 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3058 3090 4549 4581 7290 7322 3058 Size: 32 1459 32 2709 32 3066 Fragments arranged by size: 3066 2709 1459 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7330 XbaI T'CTAG_A Cuts at: 4666 4666 Size: 7330 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4484 4484 Size: 7330 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7330 XmaI C'CCGG_G Cuts at: 1870 2643 3300 4655 1870 Size: 773 657 1355 4545 Fragments arranged by size: 4545 1355 773 657 XmnI GAAnn'nnTTC Cuts at: 2456 5459 2456 Size: 3003 4327 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2102 2362 5138 276 Size: 53 83 186 936 568 260 2776 2468 Fragments arranged by size: 2776 2468 936 568 260 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfoI SrfI SwaI