Restriction map for
pFN21AB7670
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI MaeI MamI MfeI MflI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tth111I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 463 GAATTC [GA]^AATT[CT]
AlwI 291 GGATC GGATC(4/5)
ApaI 112 GGGCCC GGGCC^C
ApoI 463 GAATTC [GA]^AATT[CT]
BalI 959 TGGCCA TGG^CCA
BanI 839 GGCACC G^G[CT][GA]CC
BbvCI 1080 CCTCAGC CCTCAGC(-5/-2)
BlpI 525 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 525 GCTCAGC GC^T[ACGT]AGC
BsaI 717 GGTCTC GGTCTC(1/5)
BsgI 342 GTGCAG GTGCAG(16/14)
BsmBI 763 GAGACG CGTCTC(1/5)
BspMI 521 ACCTGC ACCTGC(4/8)
BsrBI 439 GAGCGG CCGCTC(-3/-3)
BsrI 753 ACTGG ACTGG(1/-1)
BssSI 229 CACGAG CACGAG(-5/-1)
CelII 525 GCTCAGC GC^T[ACGT]AGC
EcoRI 463 GAATTC G^AATTC
HaeII 390 GGCGCT [GA]GCGC^[CT]
HincII 211 GTCAAC GT[CT]^[GA]AC
HindII 211 GTCAAC GT[CT]^[GA]AC
KspI 646 CCGCGG CCGC^GG
MaeII 1109 ACGT A^CGT
MaeIII 500 GTGAC ^GT[ACGT]AC
MluNI 959 TGGCCA TGG^CCA
MscI 959 TGGCCA TGG^CCA
MseI 1072 TTAA T^TAA
MslI 81 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 591 GCCGGC GCC^GGC
NcoI 962 CCATGG C^CATGG
NgoMIV 591 GCCGGC G^CCGGC
PflMI 870 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1120 CTGCAG CTGCA^G
SacII 646 CCGCGG CCGC^GG
SbfI 1119 CCTGCAGG CCTGCA^GG
SfcI 1120 CTGCAG C^T[GA][CT]AG
SmaI 109 CCCGGG CCC^GGG
SmlI 472 CTCAAG C^T[CT][GA]AG
Sse8387I 1119 CCTGCAGG CCTGCA^GG
StuI 704 AGGCCT AGG^CCT
Tru9I 1072 TTAA T^TAA
Tsp45I 500 GTGAC ^GT[GC]AC
Tsp509I 464 AATT ^AATT
Van91I 870 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 109 CCCGGG C^CCGGG
XmaI 109 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 303 GTAC GT^AC
405 GTAC
AspHI 94 GAGCAC G[AT]GC[AT]^C
469 GTGCTC
AvaI 19 CCCGAG C^[CT]CG[GA]G
109 CCCGGG
BglI 697 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
974 GCCCCTACGGC
BpmI 28 CTGGAG CTGGAG(16/14)
394 CTGGAG
BsiEI 192 CGACCG CG[GA][CT]^CG
1146 CGACCG
BsiHKAI 94 GAGCAC G[AT]GC[AT]^C
469 GTGCTC
BsmFI 581 GGGAC GGGAC(10/14)
17 GTCCC
BsoBI 19 CCCGAG C^[CT]CG[GA]G
109 CCCGGG
BsrFI 591 GCCGGC [GA]^CCGG[CT]
1148 ACCGGC
BstAPI 96 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
344 GCAGCGGCTGC
BstXI 65 CCAAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
896 CCAGCCGCATGG
Cfr10I 591 GCCGGC [GA]^CCGG[CT]
1148 ACCGGC
DsaI 646 CCGCGG C^C[GA][CT]GG
962 CCATGG
EcoT14I 596 CCAAGG C^C[AT][AT]GG
962 CCATGG
RsaI 303 GTAC GT^AC
405 GTAC
StyI 596 CCAAGG C^C[AT][AT]GG
962 CCATGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII SapI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I