Restriction map for
pFN21AB0966
(Circular) (Six-base) MAPSORT of: pFN21AB0966.seq Check: 2730 from: 1 to: 5986
pFN21AB0966 5986 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2626 2710 2878 4049 5433 5701 1632
Size: 994 84 168 1171 1384 268 1917
Fragments arranged by size:
1917 1384 1171 994 268 168 84
AflII C'TTAA_G
Cuts at: 820 1017 2320 3235 820
Size: 197 1303 915 3571
Fragments arranged by size:
3571 1303 915 197
AflIII A'CryG_T
Cuts at: 1203 2110 2240 2402 4885 5862 1203
Size: 907 130 162 2483 977 1327
Fragments arranged by size:
2483 1327 977 907 162 130
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 2241 3105 1166
Size: 1075 864 4047
Fragments arranged by size:
4047 1075 864
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 2042 3729 5301 2042
Size: 1687 1572 2727
Fragments arranged by size:
2727 1687 1572
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 2592 3012 3056 3295 3521 3673 5782 1493
Size: 1099 420 44 239 226 152 2109 1697
Fragments arranged by size:
2109 1697 1099 420 239 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 3311 1870
Size: 73 1368 4545
Fragments arranged by size:
4545 1368 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3255 3307 4762 618
Size: 325 217 627 1468 52 1455 1842
Fragments arranged by size:
1842 1468 1455 627 325 217 52
BanII G_rGCy'C
Cuts at: 1391 3305 1391
Size: 1914 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2483 2884 928
Size: 971 584 401 4030
Fragments arranged by size:
4030 971 584 401
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BfrBI ATG'CAT
Cuts at: 2507 2527 2507
Size: 20 5966
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4597 136
Size: 107 122 71 631 605 2925 1525
Fragments arranged by size:
2925 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 3403 3403
Size: 5986
Bme1580I G_kGCm'C
Cuts at: 1790 4047 5203 1790
Size: 2257 1156 2573
Fragments arranged by size:
2573 2257 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3717 4675 442
Size: 416 53 83 399 24 2300 958 1753
Fragments arranged by size:
2300 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2706 2790 2958 3210 4646 1239
Size: 1467 84 168 252 1436 2579
Fragments arranged by size:
2579 1467 1436 252 168 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4111 4976 5274 4111
Size: 865 298 4823
Fragments arranged by size:
4823 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4649 882
Size: 617 3150 2219
Fragments arranged by size:
3150 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2403 3777 493
Size: 711 1199 1374 2702
Fragments arranged by size:
2702 1374 1199 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3793 4175 4810 275
Size: 53 83 186 936 2260 382 635 1451
Fragments arranged by size:
2260 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4419 4817 5091 5238 5649 4419
Size: 398 274 147 411 4756
Fragments arranged by size:
4756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5761
Fragments arranged by size:
5761 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 1186
Size: 1240 4746
BseYI C'CCAG_C
Cuts at: 5189 5685 5189
Size: 496 5490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2344 2557 2641 2893 3145 3229 1163
Size: 573 608 213 84 252 252 84 3920
Fragments arranged by size:
3920 608 573 252 252 213 84 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2492 4047 4132 5203 2492
Size: 1555 85 1071 3275
Fragments arranged by size:
3275 1555 1071 85
BsmI GAATG_Cn'
Cuts at: 2708 3053 3497 3590 2708
Size: 345 444 93 5104
Fragments arranged by size:
5104 444 345 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2492 3305 4047 4132 5203 1391
Size: 399 702 813 742 85 1071 2174
Fragments arranged by size:
2174 1071 813 742 702 399 85
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2120 3341 844
Size: 1276 1221 3489
Fragments arranged by size:
3489 1276 1221
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2797 4481 4655 65
Size: 1183 1549 1684 174 1396
Fragments arranged by size:
1684 1549 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4630 4817 1700
Size: 110 2820 187 2869
Fragments arranged by size:
2869 2820 187 110
BsrGI T'GTAC_A
Cuts at: 96 2833 96
Size: 2737 3249
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3048 794
Size: 2254 3732
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 3316 3683 4068 4085 5537 5623 5635 5981
Size: 1344 367 385 17 1452 86 12 346
Cuts at: 5981 1972
Size: 1977
Fragments arranged by size:
1977 1452 1344 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2221 513
Size: 552 1156 4278
Fragments arranged by size:
4278 1156 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 2109 2545 2881 2965 3217 3270 3502 4296 4316
Size: 436 336 84 252 53 232 794 20
Cuts at: 4316 2109
Size: 3779
Fragments arranged by size:
3779 794 436 336 252 232 84 53
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2779 2814 529
Size: 35 2215 35 3701
Fragments arranged by size:
3701 2215 35 35
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2576 4993 809
Size: 507 1260 2417 1802
Fragments arranged by size:
2417 1802 1260 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2980 3433 4322
Size: 54 1425 50 275 18 1150 453 889
Cuts at: 4322 5951 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1150 889 453 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 3916 1353
Size: 711 1852 3423
Fragments arranged by size:
3423 1852 711
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2626 2706 2710 2790 2878 2958 3210
Size: 393 994 80 4 80 88 80 252
Cuts at: 3210 4049 4646 5433 5701 1239
Size: 839 597 787 268 1524
Fragments arranged by size:
1524 994 839 787 597 393 268 252
88 80 80 80 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2323 2323
Size: 5986
EcoO109I rG'GnC_Cy
Cuts at: 2039 2039
Size: 5986
EcoRI G'AATT_C
Cuts at: 2592 3012 3295 3673 2592
Size: 420 283 378 4905
Fragments arranged by size:
4905 420 378 283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2591 2675 2759 2591
Size: 84 84 5818
Fragments arranged by size:
5818 84 84
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 5133 1678
Size: 3455 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 4627 4659 4701
Size: 32 135 32 485 32 2538 32 42
Cuts at: 4701 4733 1373
Size: 32 2626
Fragments arranged by size:
2626 2538 485 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3330 3576 669
Size: 988 281 1392 246 3079
Fragments arranged by size:
3079 1392 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2553 748
Size: 1805 4181
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 2346 3585 2346
Size: 1239 4747
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5100 5284 1305
Size: 3795 184 2007
Fragments arranged by size:
3795 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2982 10
Size: 54 1425 343 1150 3014
Fragments arranged by size:
3014 1425 1150 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2199 2241 3105 3205 3944 4303 4462
Size: 648 1033 42 864 100 739 359 159
Cuts at: 4462 518
Size: 2042
Fragments arranged by size:
2042 1033 864 739 648 359 159 100
42
MspA1I CmG'CkG
Cuts at: 1606 3402 4079 5227 5472 1606
Size: 1796 677 1148 245 2120
Fragments arranged by size:
2120 1796 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2221 513
Size: 552 1156 4278
Fragments arranged by size:
4278 1156 552
NdeI CA'TA_TG
Cuts at: 387 2165 3202 387
Size: 1778 1037 3171
Fragments arranged by size:
3171 1778 1037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NsiI A_TGCA'T
Cuts at: 2509 2529 2509
Size: 20 5966
NspI r_CATG'y
Cuts at: 1132 1229 2114 2244 2253 3344 4889 1132
Size: 97 885 130 9 1091 1545 2229
Fragments arranged by size:
2229 1545 1091 885 130 97 9
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 2110 2240 4885 2110
Size: 130 2645 3211
Fragments arranged by size:
3211 2645 130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5986
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2012 1613
Size: 399 5587
PsiI TTA'TAA
Cuts at: 2784 2868 3556 2784
Size: 84 688 5214
Fragments arranged by size:
5214 688 84
PstI C_TGCA'G
Cuts at: 830 3277 3338 830
Size: 2447 61 3478
Fragments arranged by size:
3478 2447 61
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 2097 3229 3273 3334 4469 5150 5341
Size: 219 1052 1132 44 61 1135 681 191
Cuts at: 5341 826
Size: 1471
Fragments arranged by size:
1471 1135 1132 1052 681 219 191 61
44
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2320 3235 4090 4991 5253 820
Size: 197 926 377 915 855 901 262 1553
Fragments arranged by size:
1553 926 915 901 855 377 262 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 2807 3910 5
Size: 47 2755 1103 2081
Fragments arranged by size:
2755 2081 1103 47
StuI AGG'CCT
Cuts at: 1517 2342 2695 2863 3115 3227 1517
Size: 825 353 168 252 112 4276
Fragments arranged by size:
4276 825 353 252 168 112
StyI C'CwwG_G
Cuts at: 513 1065 2124 2221 3425 513
Size: 552 1059 97 1204 3074
Fragments arranged by size:
3074 1204 1059 552 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2429 4013 4030 4183 4368 4813 325
Size: 1255 849 1584 17 153 185 445 1498
Fragments arranged by size:
1584 1498 1255 849 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2833 4232 5944
Size: 274 80 33 51 1443 856 1399 1712
Cuts at: 5944 96
Size: 138
Fragments arranged by size:
1712 1443 1399 856 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 2423 2555 2639 2891 4115 2423
Size: 132 84 252 1224 4294
Fragments arranged by size:
4294 1224 252 132 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI