(Circular) (Six-base) MAPSORT of: pFN21AB3008.seq Check: 6621 from: 1 to: 7171 pFN21AB3008 7171 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4981 278 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2629 4514 1937 Size: 692 1885 4594 Fragments arranged by size: 4594 1885 692 Acc65I G'GTAC_C Cuts at: 4492 4492 Size: 7171 AclI AA'CG_TT Cuts at: 1563 5298 5671 1563 Size: 3735 373 3063 Fragments arranged by size: 3735 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3059 4329 5234 6618 6886 1632 Size: 1427 1270 905 1384 268 1917 Fragments arranged by size: 1917 1427 1384 1270 905 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6974 AflIII A'CryG_T Cuts at: 1203 3968 6070 7047 1203 Size: 2765 2102 977 1327 Fragments arranged by size: 2765 2102 1327 977 AgeI A'CCGG_T Cuts at: 6002 6002 Size: 7171 AhdI GACnn_n'nnGTC Cuts at: 4104 5900 4104 Size: 1796 5375 AleI CACnn'nnGTG Cuts at: 1166 2458 2681 1166 Size: 1292 223 5656 Fragments arranged by size: 5656 1292 223 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2476 4025 1184 Size: 1292 1549 4330 Fragments arranged by size: 4330 1549 1292 AlwNI CAG_nnn'CTG Cuts at: 3380 3782 3899 3935 4914 6486 3380 Size: 402 117 36 979 1572 4065 Fragments arranged by size: 4065 1572 979 402 117 36 ApaLI G'TGCA_C Cuts at: 4260 5228 6384 4260 Size: 968 1156 5047 Fragments arranged by size: 5047 1156 968 ApoI r'AATT_y Cuts at: 1493 2383 2846 2984 4127 4480 4706 4858 6967 Size: 890 463 138 1143 353 226 152 2109 Cuts at: 6967 1493 Size: 1697 Fragments arranged by size: 2109 1697 1143 890 463 353 226 152 138 AseI AT'TA_AT Cuts at: 160 1032 5725 160 Size: 872 4693 1606 Fragments arranged by size: 4693 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 AvaI C'yCGr_G Cuts at: 1870 1943 4496 1870 Size: 73 2553 4545 Fragments arranged by size: 4545 2553 73 BamHI G'GATC_C Cuts at: 3506 4501 4868 3506 Size: 995 367 5809 Fragments arranged by size: 5809 995 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4492 5947 618 Size: 325 217 627 2705 1455 1842 Fragments arranged by size: 2705 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2650 3321 3654 4490 1391 Size: 1259 671 333 836 4072 Fragments arranged by size: 4072 1259 836 671 333 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4202 4405 928 Size: 971 2303 203 3694 Fragments arranged by size: 3694 2303 971 203 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3458 3492 5362 5396 1903 Size: 34 1521 34 1870 34 3678 Fragments arranged by size: 3678 1870 1521 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5063 6273 5063 Size: 1210 5961 BclI T'GATC_A Cuts at: 1548 2247 1548 Size: 699 6472 BfrBI ATG'CAT Cuts at: 2425 2425 Size: 7171 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5782 136 Size: 107 122 71 631 605 4110 1525 Fragments arranged by size: 4110 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2488 3358 7166 2488 Size: 870 3808 2493 Fragments arranged by size: 3808 2493 870 BlpI GC'TnA_GC Cuts at: 4588 4588 Size: 7171 Bme1580I G_kGCm'C Cuts at: 1790 4264 5232 6388 1790 Size: 2474 968 1156 2573 Fragments arranged by size: 2573 2474 1156 968 BmgBI CAC'GTC Cuts at: 1318 3971 4200 1318 Size: 2653 229 4289 Fragments arranged by size: 4289 2653 229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2025 4902 5860 Size: 416 53 83 399 24 608 2877 958 Cuts at: 5860 442 Size: 1753 Fragments arranged by size: 2877 1753 958 608 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7171 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4134 4134 Size: 7171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2399 3759 3912 3970 5831 1239 Size: 1160 1360 153 58 1861 2579 Fragments arranged by size: 2579 1861 1360 1160 153 58 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2295 5296 6161 6459 2295 Size: 3001 865 298 3007 Fragments arranged by size: 3007 3001 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3763 5834 882 Size: 617 2264 2071 2219 Fragments arranged by size: 2264 2219 2071 617 BsaAI yAC'GTr Cuts at: 493 1204 4962 493 Size: 711 3758 2702 Fragments arranged by size: 3758 2702 711 BsaBI GATnn'nnATC Cuts at: 2493 3363 7165 2493 Size: 870 3802 2499 Fragments arranged by size: 3802 2499 870 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2595 4978 5360 5995 Size: 53 83 186 936 1062 2383 382 635 Cuts at: 5995 275 Size: 1451 Fragments arranged by size: 2383 1451 1062 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4039 5604 6002 6276 6423 6834 4039 Size: 1565 398 274 147 411 4376 Fragments arranged by size: 4376 1565 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2931 2961 3880 3910 3968 Size: 30 165 30 1528 30 919 30 58 Cuts at: 3968 3998 1178 Size: 30 4351 Fragments arranged by size: 4351 1528 919 165 58 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3873 1186 Size: 2687 4484 BseYI C'CCAG_C Cuts at: 3312 3955 6374 6870 3312 Size: 643 2419 496 3613 Fragments arranged by size: 3613 2419 643 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3131 1163 Size: 573 1395 5203 Fragments arranged by size: 5203 1395 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4621 5382 6410 1540 Size: 275 186 2620 761 1028 2301 Fragments arranged by size: 2620 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2650 3654 4264 5232 5317 6388 2650 Size: 1004 610 968 85 1071 3433 Fragments arranged by size: 3433 1071 1004 968 610 85 BsmI GAATG_Cn' Cuts at: 2425 3439 4682 4775 2425 Size: 1014 1243 93 4821 Fragments arranged by size: 4821 1243 1014 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2650 3321 3654 4264 4490 5232 5317 Size: 399 860 671 333 610 226 742 85 Cuts at: 5317 6388 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 860 742 671 610 399 333 226 85 BspEI T'CCGG_A Cuts at: 6834 6834 Size: 7171 BspHI T'CATG_A Cuts at: 2761 5060 6790 2761 Size: 2299 1730 3142 Fragments arranged by size: 3142 2299 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4526 844 Size: 3682 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4118 5058 1394 Size: 429 2295 940 3507 Fragments arranged by size: 3507 2295 940 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3313 3707 5666 5840 65 Size: 1183 2065 394 1959 174 1396 Fragments arranged by size: 2065 1959 1396 1183 394 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5815 6002 1700 Size: 110 4005 187 2869 Fragments arranged by size: 4005 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4339 96 Size: 4243 2928 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7171 BssSI C'ACGA_G Cuts at: 5231 6243 5231 Size: 1012 6159 BstAPI GCAn_nnn'nTGC Cuts at: 794 4150 794 Size: 3356 3815 BstBI TT'CG_AA Cuts at: 3715 5975 3715 Size: 2260 4911 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7171 BstYI r'GATC_y Cuts at: 1972 2488 3358 3506 4501 4868 5253 5270 6722 Size: 516 870 148 995 367 385 17 1452 Cuts at: 6722 6808 6820 7166 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 995 870 516 385 367 346 148 86 17 12 BstZ17I GTA'TAC Cuts at: 2630 2630 Size: 7171 Bsu36I CC'TnA_GG Cuts at: 2600 2600 Size: 7171 BtgI C'CryG_G Cuts at: 513 1065 4139 513 Size: 552 3074 3545 Fragments arranged by size: 3545 3074 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6432 Fragments arranged by size: 6432 683 56 BtsI GCAGTG_nn' Cuts at: 2182 2407 4372 4687 5481 5501 2182 Size: 225 1965 315 794 20 3852 Fragments arranged by size: 3852 1965 794 315 225 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7171 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7136 DraI TTT'AAA Cuts at: 2394 4475 2394 Size: 2081 5090 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6178 809 Size: 507 4862 1802 Fragments arranged by size: 4862 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3030 3263 4618 Size: 54 1425 50 275 18 1200 233 1355 Cuts at: 4618 5507 7136 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1355 1200 889 275 233 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4618 1537 Size: 275 2806 4090 Fragments arranged by size: 4090 2806 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2504 2576 5101 1353 Size: 1151 72 2525 3423 Fragments arranged by size: 3423 2525 1151 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5758 6132 6278 387 Size: 1064 138 4169 374 146 1280 Fragments arranged by size: 4169 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2648 3652 2648 Size: 1004 6167 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 3059 3759 3912 3970 4329 5234 Size: 393 767 660 700 153 58 359 905 Cuts at: 5234 5831 6618 6886 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 905 787 767 700 660 597 393 359 268 153 58 EcoO109I rG'GnC_Cy Cuts at: 2131 3125 3952 2131 Size: 994 827 5350 Fragments arranged by size: 5350 994 827 EcoRI G'AATT_C Cuts at: 4480 4858 4480 Size: 378 6793 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3643 3643 Size: 7171 FspI TGC'GCA Cuts at: 5677 5677 Size: 7171 HaeII r_GCGC'y Cuts at: 1678 6318 1678 Size: 4640 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 4090 4122 5812 Size: 32 135 32 776 32 1710 32 1690 Cuts at: 5812 5844 5886 5918 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1710 1690 776 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4515 4761 669 Size: 988 281 2577 246 3079 Fragments arranged by size: 3079 2577 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7171 HpaI GTT'AAC Cuts at: 4761 4761 Size: 7171 KpnI G_GTAC'C Cuts at: 4496 4496 Size: 7171 MfeI C'AATT_G Cuts at: 3174 4770 3174 Size: 1596 5575 MluI A'CGCG_T Cuts at: 7047 7047 Size: 7171 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3911 4088 6285 6469 1305 Size: 2606 177 2197 184 2007 Fragments arranged by size: 2606 2197 2007 184 177 MscI TGG'CCA Cuts at: 10 64 1489 1832 3032 3265 10 Size: 54 1425 343 1200 233 3916 Fragments arranged by size: 3916 1425 1200 343 233 54 MslI CAynn'nnrTG Cuts at: 518 1166 2458 2681 3444 5129 5488 5647 518 Size: 648 1292 223 763 1685 359 159 2042 Fragments arranged by size: 2042 1685 1292 763 648 359 223 159 MspA1I CmG'CkG Cuts at: 1606 3217 3857 4587 5264 6412 6657 1606 Size: 1611 640 730 677 1148 245 2120 Fragments arranged by size: 2120 1611 1148 730 677 640 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6619 NdeI CA'TA_TG Cuts at: 387 3289 387 Size: 2902 4269 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7171 NotI GC'GGCC_GC Cuts at: 4618 4618 Size: 7171 NsiI A_TGCA'T Cuts at: 2427 2427 Size: 7171 NspI r_CATG'y Cuts at: 1132 1229 4529 6074 1132 Size: 97 3300 1545 2229 Fragments arranged by size: 3300 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2666 1348 Size: 1318 5853 PciI A'CATG_T Cuts at: 6070 6070 Size: 7171 PflMI CCAn_nnn'nTGG Cuts at: 1108 2833 3470 3782 1108 Size: 1725 637 312 4497 Fragments arranged by size: 4497 1725 637 312 PfoI T'CCnGG_A Cuts at: 2226 2226 Size: 7171 PmeI GTTT'AAAC Cuts at: 4475 4475 Size: 7171 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5240 5272 6753 6785 5240 Size: 32 1481 32 5626 Fragments arranged by size: 5626 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3125 3952 3125 Size: 827 6344 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7171 PsiI TTA'TAA Cuts at: 4741 4741 Size: 7171 PstI C_TGCA'G Cuts at: 830 3070 4523 830 Size: 2240 1453 3478 Fragments arranged by size: 3478 2240 1453 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7171 PvuII CAG'CTG Cuts at: 3217 3857 3217 Size: 640 6531 SacI G_AGCT'C Cuts at: 2650 3654 2650 Size: 1004 6167 SalI G'TCGA_C Cuts at: 1936 4513 1936 Size: 2577 4594 SbfI CC_TGCA'GG Cuts at: 4523 4523 Size: 7171 ScaI AGT'ACT Cuts at: 3147 5419 7131 3147 Size: 2272 1712 3187 Fragments arranged by size: 3187 2272 1712 SfcI C'TryA_G Cuts at: 826 1045 3066 4324 4519 5654 6335 6526 826 Size: 219 2021 1258 195 1135 681 191 1471 Fragments arranged by size: 2021 1471 1258 1135 681 219 195 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7171 SmaI CCC'GGG Cuts at: 1872 4498 1872 Size: 2626 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2310 5275 6176 6438 820 Size: 197 926 367 2965 901 262 1553 Fragments arranged by size: 2965 1553 926 901 367 262 197 SnaBI TAC'GTA Cuts at: 493 4962 493 Size: 4469 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7171 SphI G_CATG'C Cuts at: 1132 4529 1132 Size: 3397 3774 SspI AAT'ATT Cuts at: 5 52 5095 5 Size: 47 5043 2081 Fragments arranged by size: 5043 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7171 StyI C'CwwG_G Cuts at: 513 1065 2292 2731 2750 3520 4610 513 Size: 552 1227 439 19 770 1090 3074 Fragments arranged by size: 3074 1227 1090 770 552 439 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2656 2693 4062 5198 5215 5368 5553 Size: 1255 1076 37 1369 1136 17 153 185 Cuts at: 5553 5998 325 Size: 445 1498 Fragments arranged by size: 1498 1369 1255 1136 1076 445 185 153 37 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3145 4339 5417 Size: 274 80 33 51 1443 1168 1194 1078 Cuts at: 5417 7129 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1194 1168 1078 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3631 3663 7131 7163 3631 Size: 32 3468 32 3639 Fragments arranged by size: 3639 3468 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2168 1316 Size: 852 6319 XbaI T'CTAG_A Cuts at: 4507 4507 Size: 7171 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7171 XmaI C'CCGG_G Cuts at: 1870 4496 1870 Size: 2626 4545 XmnI GAAnn'nnTTC Cuts at: 4127 5300 4127 Size: 1173 5998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4979 276 Size: 53 83 186 936 3445 2468 Fragments arranged by size: 3445 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI