(Circular) (Six-base) MAPSORT of: pFN21AB7305.seq Check: 4814 from: 1 to: 7135 pFN21AB7305 7135 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4945 278 Size: 53 83 186 936 3409 2468 Fragments arranged by size: 3409 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4478 1937 Size: 2541 4594 Acc65I G'GTAC_C Cuts at: 4456 4456 Size: 7135 AclI AA'CG_TT Cuts at: 1563 5262 5635 1563 Size: 3699 373 3063 Fragments arranged by size: 3699 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3068 3509 5198 6582 6850 1632 Size: 1436 441 1689 1384 268 1917 Fragments arranged by size: 1917 1689 1436 1384 441 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6938 AflIII A'CryG_T Cuts at: 1203 6034 7011 1203 Size: 4831 977 1327 Fragments arranged by size: 4831 1327 977 AgeI A'CCGG_T Cuts at: 5966 5966 Size: 7135 AhdI GACnn_n'nnGTC Cuts at: 3596 5864 3596 Size: 2268 4867 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7135 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7135 AlwNI CAG_nnn'CTG Cuts at: 4878 6450 4878 Size: 1572 5563 ApaLI G'TGCA_C Cuts at: 2538 5192 6348 2538 Size: 2654 1156 3325 Fragments arranged by size: 3325 2654 1156 ApoI r'AATT_y Cuts at: 1493 4444 4670 4822 6931 1493 Size: 2951 226 152 2109 1697 Fragments arranged by size: 2951 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2813 5689 160 Size: 872 1781 2876 1606 Fragments arranged by size: 2876 1781 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7135 AvaI C'yCGr_G Cuts at: 1870 1943 4402 4460 1870 Size: 73 2459 58 4545 Fragments arranged by size: 4545 2459 73 58 BamHI G'GATC_C Cuts at: 4465 4832 4465 Size: 367 6768 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4128 4456 5911 618 Size: 325 217 627 2341 328 1455 1842 Fragments arranged by size: 2341 1842 1455 627 328 325 217 BanII G_rGCy'C Cuts at: 1391 2090 2919 4125 4454 1391 Size: 699 829 1206 329 4072 Fragments arranged by size: 4072 1206 829 699 329 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4353 928 Size: 971 2454 3710 Fragments arranged by size: 3710 2454 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5326 5360 1903 Size: 34 3389 34 3678 Fragments arranged by size: 3678 3389 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2487 5027 6237 2487 Size: 2540 1210 3385 Fragments arranged by size: 3385 2540 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7135 BfrBI ATG'CAT Cuts at: 2963 2963 Size: 7135 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4137 4146 5746 Size: 107 122 71 631 605 2465 9 1600 Cuts at: 5746 136 Size: 1525 Fragments arranged by size: 2465 1600 1525 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 2315 7130 2315 Size: 4815 2320 BlpI GC'TnA_GC Cuts at: 2084 4011 4552 2084 Size: 1927 541 4667 Fragments arranged by size: 4667 1927 541 Bme1580I G_kGCm'C Cuts at: 1790 2542 5196 6352 1790 Size: 752 2654 1156 2573 Fragments arranged by size: 2654 2573 1156 752 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7135 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3654 4866 5824 Size: 416 53 83 399 24 2237 1212 958 Cuts at: 5824 442 Size: 1753 Fragments arranged by size: 2237 1753 1212 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7135 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2109 2109 Size: 7135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4379 5795 1239 Size: 3140 1416 2579 Fragments arranged by size: 3140 2579 1416 Bpu10I CC'TnA_GC Cuts at: 4124 4124 Size: 7135 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5260 6125 6423 5260 Size: 865 298 5972 Fragments arranged by size: 5972 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4355 5798 882 Size: 617 2856 1443 2219 Fragments arranged by size: 2856 2219 1443 617 BsaAI yAC'GTr Cuts at: 493 1204 4926 493 Size: 711 3722 2702 Fragments arranged by size: 3722 2702 711 BsaBI GATnn'nnATC Cuts at: 7129 7129 Size: 7135 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4942 5324 5959 275 Size: 53 83 186 936 3409 382 635 1451 Fragments arranged by size: 3409 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5568 5966 6240 6387 6798 5568 Size: 398 274 147 411 5905 Fragments arranged by size: 5905 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6910 Fragments arranged by size: 6910 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7135 BseYI C'CCAG_C Cuts at: 6338 6834 6338 Size: 496 6639 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3846 3869 1163 Size: 573 2110 23 4429 Fragments arranged by size: 4429 2110 573 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4585 5346 6374 1540 Size: 275 186 2584 761 1028 2301 Fragments arranged by size: 2584 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2090 2542 2919 4125 5196 5281 6352 2090 Size: 452 377 1206 1071 85 1071 2873 Fragments arranged by size: 2873 1206 1071 1071 452 377 85 BsmI GAATG_Cn' Cuts at: 3929 4646 4739 3929 Size: 717 93 6325 Fragments arranged by size: 6325 717 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2542 2919 4125 4454 5196 5281 Size: 399 300 452 377 1206 329 742 85 Cuts at: 5281 6352 1391 Size: 1071 2174 Fragments arranged by size: 2174 1206 1071 742 452 399 377 329 300 85 BspEI T'CCGG_A Cuts at: 6798 6798 Size: 7135 BspHI T'CATG_A Cuts at: 4117 5024 6754 4117 Size: 907 1730 4498 Fragments arranged by size: 4498 1730 907 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4490 844 Size: 3646 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4396 5022 1394 Size: 429 2573 626 3507 Fragments arranged by size: 3507 2573 626 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2619 3537 4005 5630 5804 65 Size: 1183 1371 918 468 1625 174 1396 Fragments arranged by size: 1625 1396 1371 1183 918 468 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5779 5966 1700 Size: 110 3969 187 2869 Fragments arranged by size: 3969 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2923 96 Size: 2827 4308 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7135 BssSI C'ACGA_G Cuts at: 5195 6207 5195 Size: 1012 6123 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7135 BstBI TT'CG_AA Cuts at: 3808 5939 3808 Size: 2131 5004 BstEII G'GTnAC_C Cuts at: 1184 2008 1184 Size: 824 6311 BstXI CCAn_nnnn'nTGG Cuts at: 2149 4082 2149 Size: 1933 5202 BstYI r'GATC_y Cuts at: 1972 2315 4465 4832 5217 5234 6686 6772 6784 Size: 343 2150 367 385 17 1452 86 12 Cuts at: 6784 7130 1972 Size: 346 1977 Fragments arranged by size: 2150 1977 1452 385 367 346 343 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5644 Fragments arranged by size: 5644 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6396 Fragments arranged by size: 6396 683 56 BtsI GCAGTG_nn' Cuts at: 2542 4323 4651 5445 5465 2542 Size: 1781 328 794 20 4212 Fragments arranged by size: 4212 1781 794 328 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7135 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7100 DraI TTT'AAA Cuts at: 2845 3321 4439 2845 Size: 476 1118 5541 Fragments arranged by size: 5541 1118 476 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6142 809 Size: 507 4826 1802 Fragments arranged by size: 4826 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 4582 5471 Size: 54 1425 50 275 18 309 2443 889 Cuts at: 5471 7100 8 Size: 1629 43 Fragments arranged by size: 2443 1629 1425 889 309 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4582 1537 Size: 275 2770 4090 Fragments arranged by size: 4090 2770 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2353 3270 3476 3805 4405 5065 1353 Size: 1000 917 206 329 600 660 3423 Fragments arranged by size: 3423 1000 917 660 600 329 206 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5722 6096 6242 387 Size: 1064 138 4133 374 146 1280 Fragments arranged by size: 4133 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2088 2917 4123 2088 Size: 829 1206 5100 Fragments arranged by size: 5100 1206 829 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3068 3509 4379 5198 5795 6582 6850 Size: 393 1436 441 870 819 597 787 268 Cuts at: 6850 1239 Size: 1524 Fragments arranged by size: 1524 1436 870 819 787 597 441 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2672 2672 Size: 7135 EcoRI G'AATT_C Cuts at: 4444 4822 4444 Size: 378 6757 EcoRV GAT'ATC Cuts at: 3031 3031 Size: 7135 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2684 3896 4058 4070 2684 Size: 1212 162 12 5749 Fragments arranged by size: 5749 1212 162 12 FspI TGC'GCA Cuts at: 5641 5641 Size: 7135 HaeII r_GCGC'y Cuts at: 1678 3628 6282 1678 Size: 1950 2654 2531 Fragments arranged by size: 2654 2531 1950 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2128 2160 2291 2323 2485 Size: 32 135 32 556 32 131 32 162 Cuts at: 2485 2517 3582 3614 4196 4228 5776 5808 5850 Size: 32 1065 32 582 32 1548 32 42 Cuts at: 5850 5882 1373 Size: 32 2626 Fragments arranged by size: 2626 1548 1065 582 556 162 135 131 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4189 4479 4725 669 Size: 988 281 2251 290 246 3079 Fragments arranged by size: 3079 2251 988 290 281 246 HindIII A'AGCT_T Cuts at: 748 2790 3233 4040 748 Size: 2042 443 807 3843 Fragments arranged by size: 3843 2042 807 443 HpaI GTT'AAC Cuts at: 4725 4725 Size: 7135 KpnI G_GTAC'C Cuts at: 4460 4460 Size: 7135 MfeI C'AATT_G Cuts at: 3009 4734 3009 Size: 1725 5410 MluI A'CGCG_T Cuts at: 7011 7011 Size: 7135 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2737 3387 6249 6433 1305 Size: 1432 650 2862 184 2007 Fragments arranged by size: 2862 2007 1432 650 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 10 Size: 54 1425 343 309 5004 Fragments arranged by size: 5004 1425 343 309 54 MslI CAynn'nnrTG Cuts at: 518 1166 3542 5093 5452 5611 518 Size: 648 2376 1551 359 159 2042 Fragments arranged by size: 2376 2042 1551 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4551 5228 6376 6621 1606 Size: 2945 677 1148 245 2120 Fragments arranged by size: 2945 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5644 Fragments arranged by size: 5644 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7135 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7135 NotI GC'GGCC_GC Cuts at: 4582 4582 Size: 7135 NsiI A_TGCA'T Cuts at: 2965 2965 Size: 7135 NspI r_CATG'y Cuts at: 1132 1229 4493 6038 1132 Size: 97 3264 1545 2229 Fragments arranged by size: 3264 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7135 PciI A'CATG_T Cuts at: 6034 6034 Size: 7135 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7135 PmeI GTTT'AAAC Cuts at: 4439 4439 Size: 7135 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5204 5236 6717 6749 5204 Size: 32 1481 32 5590 Fragments arranged by size: 5590 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7135 PsiI TTA'TAA Cuts at: 4705 4705 Size: 7135 PstI C_TGCA'G Cuts at: 830 4487 830 Size: 3657 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7135 SacI G_AGCT'C Cuts at: 2090 2919 4125 2090 Size: 829 1206 5100 Fragments arranged by size: 5100 1206 829 SalI G'TCGA_C Cuts at: 1936 4477 1936 Size: 2541 4594 SapI GCTCTTCn'nnn_ Cuts at: 2353 3270 3805 2353 Size: 917 535 5683 Fragments arranged by size: 5683 917 535 SbfI CC_TGCA'GG Cuts at: 4487 4487 Size: 7135 ScaI AGT'ACT Cuts at: 5383 7095 5383 Size: 1712 5423 SfcI C'TryA_G Cuts at: 826 1045 3386 3836 4483 5618 6299 6490 826 Size: 219 2341 450 647 1135 681 191 1471 Fragments arranged by size: 2341 1471 1135 681 647 450 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7135 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7135 SmaI CCC'GGG Cuts at: 1872 4462 1872 Size: 2590 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5239 6140 6402 820 Size: 197 926 3296 901 262 1553 Fragments arranged by size: 3296 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4926 493 Size: 4433 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7135 SphI G_CATG'C Cuts at: 1132 4493 1132 Size: 3361 3774 SspI AAT'ATT Cuts at: 5 52 3514 5059 5 Size: 47 3462 1545 2081 Fragments arranged by size: 3462 2081 1545 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7135 StyI C'CwwG_G Cuts at: 513 1065 2004 2668 4574 513 Size: 552 939 664 1906 3074 Fragments arranged by size: 3074 1906 939 664 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2092 3679 5162 5179 5332 5517 5962 Size: 1255 512 1587 1483 17 153 185 445 Cuts at: 5962 325 Size: 1498 Fragments arranged by size: 1587 1498 1483 1255 512 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2923 5381 7093 Size: 274 80 33 51 1443 946 2458 1712 Cuts at: 7093 96 Size: 138 Fragments arranged by size: 2458 1712 1443 946 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7095 7127 7095 Size: 32 7103 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7135 XbaI T'CTAG_A Cuts at: 4471 4471 Size: 7135 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7135 XmaI C'CCGG_G Cuts at: 1870 4460 1870 Size: 2590 4545 XmnI GAAnn'nnTTC Cuts at: 2481 5264 2481 Size: 2783 4352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4943 276 Size: 53 83 186 936 3409 2468 Fragments arranged by size: 3409 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI