Restriction map for
pFN21AB4713
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII Acc65I AccI AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BbvCI BciVI
BlnI BlpI Bpu1102I BsaBI BsaI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI DraI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRV
EcoT22I FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
SalI SbfI ScaI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 1168 TCCGGA T^CCGGA
AflII 2059 CTTAAG C^TTAAG
AspHI 1393 GAGCTC G[AT]GC[AT]^C
AspI 937 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 15 GGATCC G^GATCC
BbrPI 1306 CACGTG CAC^GTG
BcgI 1518 CGAGGAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1067 TGATCA T^GATCA
BfaI 1717 CTAG C^TAG
BfrI 2059 CTTAAG C^TTAAG
BglII 539 AGATCT A^GATCT
BsaWI 1168 TCCGGA [AT]^CCGG[AT]
BseAI 1168 TCCGGA T^CCGGA
BsgI 1942 GTGCAG GTGCAG(16/14)
BsiEI 1123 CGGCCG CG[GA][CT]^CG
BsiHKAI 1393 GAGCTC G[AT]GC[AT]^C
BspEI 1168 TCCGGA T^CCGGA
BspMI 247 GCAGGT ACCTGC(4/8)
CpoI 1170 CGGACCG CG^G[AT]CCG
EagI 1123 CGGCCG C^GGCCG
EclXI 1123 CGGCCG C^GGCCG
Eco52I 1123 CGGCCG C^GGCCG
EcoRI 374 GAATTC G^AATTC
FbaI 1067 TGATCA T^GATCA
FseI 42 GGCCGGCC GGCCGG^CC
KspI 1265 CCGCGG CCGC^GG
MaeI 1717 CTAG C^TAG
MroI 1168 TCCGGA T^CCGGA
PaeR7I 1671 CTCGAG C^TCGAG
PflFI 937 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1306 CACGTG CAC^GTG
PmlI 1306 CACGTG CAC^GTG
PshAI 1503 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1135 CAGCTG CAG^CTG
RsrII 1170 CGGACCG CG^G[AT]CCG
SacI 1393 GAGCTC GAGCT^C
SacII 1265 CCGCGG CCGC^GG
SexAI 2093 ACCTGGT A^CC[AT]GGT
SfiI 1872 GGCCTACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 116 CGCCGGCG C[GA]^CCGG[CT]G
Tth111I 937 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 140 CCAGCCCGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1671 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AcsI 374 GAATTC [GA]^AATT[CT]
2110 AAATTT
AlwNI 725 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2089 CAGGACCTG
ApoI 374 GAATTC [GA]^AATT[CT]
2110 AAATTT
BalI 393 TGGCCA TGG^CCA
1302 TGGCCA
BpmI 1613 CTGGAG CTGGAG(16/14)
1627 CTGGAG
BsaAI 1306 CACGTG [CT]AC^GT[GA]
2044 TACGTG
BsmBI 1616 GAGACG CGTCTC(1/5)
1636 GAGACG
BssSI 1201 CACGAG CACGAG(-5/-1)
473 CTCGTG
MluNI 393 TGGCCA TGG^CCA
1302 TGGCCA
MscI 393 TGGCCA TGG^CCA
1302 TGGCCA
SapI 1833 GCTCTTC GCTCTTC(1/4)
2076 GCTCTTC
StuI 575 AGGCCT AGG^CCT
1973 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI ApaI AvaI AvaII
BanI BanII BbeI BbsI BbvI
BcnI BglI BmyI BpuAI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI