(Circular) (Six-base) MAPSORT of: pFN21AB4713.seq Check: 4663 from: 1 to: 7033 pFN21AB4713 7033 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2243 2243 Size: 7033 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4843 278 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4376 1937 Size: 2439 4594 Acc65I G'GTAC_C Cuts at: 4354 4354 Size: 7033 AclI AA'CG_TT Cuts at: 1563 5160 5533 1563 Size: 3597 373 3063 Fragments arranged by size: 3597 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2369 2724 2829 2994 3687 5096 6480 6748 Size: 737 355 105 165 693 1409 1384 268 Cuts at: 6748 1632 Size: 1917 Fragments arranged by size: 1917 1409 1384 737 693 355 268 165 105 AflII C'TTAA_G Cuts at: 820 1017 4064 820 Size: 197 3047 3789 Fragments arranged by size: 3789 3047 197 AflIII A'CryG_T Cuts at: 1203 5932 6909 1203 Size: 4729 977 1327 Fragments arranged by size: 4729 1327 977 AgeI A'CCGG_T Cuts at: 5864 5864 Size: 7033 AhdI GACnn_n'nnGTC Cuts at: 5762 5762 Size: 7033 AleI CACnn'nnGTG Cuts at: 1166 2246 2534 1166 Size: 1080 288 5665 Fragments arranged by size: 5665 1080 288 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7033 AlwNI CAG_nnn'CTG Cuts at: 2735 4099 4776 6348 2735 Size: 1364 677 1572 3420 Fragments arranged by size: 3420 1572 1364 677 ApaI G_GGCC'C Cuts at: 2170 2204 3713 2170 Size: 34 1509 5490 Fragments arranged by size: 5490 1509 34 ApaLI G'TGCA_C Cuts at: 5090 6246 5090 Size: 1156 5877 ApoI r'AATT_y Cuts at: 1493 2379 4115 4342 4568 4720 6829 1493 Size: 886 1736 227 226 152 2109 1697 Fragments arranged by size: 2109 1736 1697 886 227 226 152 AseI AT'TA_AT Cuts at: 160 1032 5587 160 Size: 872 4555 1606 Fragments arranged by size: 4555 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 AvaI C'yCGr_G Cuts at: 1870 1943 2054 2149 2169 2197 2609 2642 3155 Size: 73 111 95 20 28 412 33 513 Cuts at: 3155 3605 3676 4358 1870 Size: 450 71 682 4545 Fragments arranged by size: 4545 682 513 450 412 111 95 73 71 33 28 20 BamHI G'GATC_C Cuts at: 2020 4363 4730 2020 Size: 2343 367 4323 Fragments arranged by size: 4323 2343 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2073 2125 2159 3731 4354 Size: 325 217 627 286 52 34 1572 623 Cuts at: 4354 5809 618 Size: 1455 1842 Fragments arranged by size: 1842 1572 1455 627 623 325 286 217 52 34 BanII G_rGCy'C Cuts at: 1391 2145 2170 2204 2423 2827 3402 3446 3594 Size: 754 25 34 219 404 575 44 148 Cuts at: 3594 3631 3713 4352 1391 Size: 37 82 639 4072 Fragments arranged by size: 4072 754 639 575 404 219 148 82 44 37 34 25 BbeI G_GCGC'C Cuts at: 2129 2163 3735 2129 Size: 34 1572 5427 Fragments arranged by size: 5427 1572 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2906 2982 3678 928 Size: 971 1007 76 696 4283 Fragments arranged by size: 4283 1007 971 696 76 BbvCI CC'TCA_GC Cuts at: 4329 4329 Size: 7033 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3512 3546 5224 5258 1903 Size: 34 1575 34 1678 34 3678 Fragments arranged by size: 3678 1678 1575 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4925 6135 4925 Size: 1210 5823 BclI T'GATC_A Cuts at: 1548 3072 1548 Size: 1524 5509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2123 3315 3428 Size: 107 122 71 631 605 451 1192 113 Cuts at: 3428 3541 3546 3884 5644 136 Size: 113 5 338 1760 1525 Fragments arranged by size: 1760 1525 1192 631 605 451 338 122 113 113 107 71 5 BglII A'GATC_T Cuts at: 2544 7028 2544 Size: 4484 2549 BlpI GC'TnA_GC Cuts at: 4450 4450 Size: 7033 Bme1580I G_kGCm'C Cuts at: 1790 2170 2204 3713 5094 6250 1790 Size: 380 34 1509 1381 1156 2573 Fragments arranged by size: 2573 1509 1381 1156 380 34 BmgBI CAC'GTC Cuts at: 1318 2404 1318 Size: 1086 5947 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2056 3847 4764 Size: 416 53 83 399 24 639 1791 917 Cuts at: 4764 5722 442 Size: 958 1753 Fragments arranged by size: 1791 1753 958 917 639 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7033 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3478 3478 Size: 7033 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3639 3653 5693 1239 Size: 2400 14 2040 2579 Fragments arranged by size: 2579 2400 2040 14 Bpu10I CC'TnA_GC Cuts at: 2069 4329 2069 Size: 2260 4773 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4013 5158 6023 6321 4013 Size: 1145 865 298 4725 Fragments arranged by size: 4725 1145 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5696 882 Size: 617 4197 2219 Fragments arranged by size: 4197 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3313 4051 4824 493 Size: 711 2109 738 773 2702 Fragments arranged by size: 2702 2109 773 738 711 BsaBI GATnn'nnATC Cuts at: 7027 7027 Size: 7033 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2105 2126 2160 2180 Size: 53 83 186 936 572 21 34 20 Cuts at: 2180 3420 3513 3644 3732 4840 5222 5857 275 Size: 1240 93 131 88 1108 382 635 1451 Fragments arranged by size: 1451 1240 1108 936 635 572 382 186 131 93 88 83 53 34 21 20 BsaWI w'CCGG_w Cuts at: 3173 5466 5864 6138 6285 6696 3173 Size: 2293 398 274 147 411 3510 Fragments arranged by size: 3510 2293 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2770 2800 1178 Size: 30 165 30 1367 30 5411 Fragments arranged by size: 5411 1367 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 2627 2817 3386 3415 3452 3518 3539 Size: 1255 186 190 569 29 37 66 21 Cuts at: 3539 4090 1186 Size: 551 4129 Fragments arranged by size: 4129 1255 569 551 190 186 66 37 29 21 BseYI C'CCAG_C Cuts at: 2135 2144 2431 3191 3479 3594 6236 6732 2135 Size: 9 287 760 288 115 2642 496 2436 Fragments arranged by size: 2642 2436 760 496 288 287 115 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3968 1163 Size: 573 2232 4228 Fragments arranged by size: 4228 2232 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3131 4483 5244 6272 1540 Size: 275 186 1130 1352 761 1028 2301 Fragments arranged by size: 2301 1352 1130 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3402 5094 5179 6250 3402 Size: 1692 85 1071 4185 Fragments arranged by size: 4185 1692 1071 85 BsmI GAATG_Cn' Cuts at: 4544 4637 4544 Size: 93 6940 BsmBI CGTCTCn'nnnn_ Cuts at: 3615 3635 3615 Size: 20 7013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2170 2204 2423 2827 3402 3446 Size: 399 355 25 34 219 404 575 44 Cuts at: 3446 3594 3631 3713 4352 5094 5179 6250 1391 Size: 148 37 82 639 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 639 575 404 399 355 219 148 85 82 44 37 34 25 BspEI T'CCGG_A Cuts at: 3173 6696 3173 Size: 3523 3510 BspHI T'CATG_A Cuts at: 4922 6652 4922 Size: 1730 5303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2243 4388 844 Size: 1399 2145 3489 Fragments arranged by size: 3489 2145 1399 BsrBI CCG'CTC Cuts at: 1394 1823 4920 1394 Size: 429 3097 3507 Fragments arranged by size: 3507 3097 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5528 5702 65 Size: 1183 4280 174 1396 Fragments arranged by size: 4280 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 2122 2192 3130 3540 5677 5864 Size: 110 238 74 70 938 410 2137 187 Cuts at: 5864 1700 Size: 2869 Fragments arranged by size: 2869 2137 938 410 238 187 110 74 70 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7033 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7033 BssSI C'ACGA_G Cuts at: 2478 3206 5093 6105 2478 Size: 728 1887 1012 3406 Fragments arranged by size: 3406 1887 1012 728 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7033 BstBI TT'CG_AA Cuts at: 5837 5837 Size: 7033 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7033 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2269 3199 3487 2248 Size: 21 930 288 5794 Fragments arranged by size: 5794 930 288 21 BstYI r'GATC_y Cuts at: 1972 2020 2544 2990 4363 4730 5115 5132 6584 Size: 48 524 446 1373 367 385 17 1452 Cuts at: 6584 6670 6682 7028 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1373 524 446 385 367 346 86 48 17 12 Bsu36I CC'TnA_GG Cuts at: 2352 2424 3289 3365 2352 Size: 72 865 76 6020 Fragments arranged by size: 6020 865 76 72 BtgI C'CryG_G Cuts at: 513 1065 2004 2226 2268 2529 3125 3264 3270 Size: 552 939 222 42 261 596 139 6 Cuts at: 3270 3656 513 Size: 386 3890 Fragments arranged by size: 3890 939 596 552 386 261 222 139 42 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6294 Fragments arranged by size: 6294 683 56 BtsI GCAGTG_nn' Cuts at: 3743 3951 4549 5343 5363 3743 Size: 208 598 794 20 5413 Fragments arranged by size: 5413 794 598 208 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7033 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6998 DraI TTT'AAA Cuts at: 4337 4337 Size: 7033 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6040 809 Size: 507 4724 1802 Fragments arranged by size: 4724 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2046 2398 Size: 54 1425 50 275 18 177 39 352 Cuts at: 2398 3128 3144 3267 3307 3552 3699 3891 4480 Size: 730 16 123 40 245 147 192 589 Cuts at: 4480 5369 6998 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 730 589 352 275 245 192 177 147 123 54 50 43 40 39 18 16 EagI C'GGCC_G Cuts at: 1537 1812 3128 4480 1537 Size: 275 1316 1352 4090 Fragments arranged by size: 4090 1352 1316 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3845 4088 4163 4963 1353 Size: 2492 243 75 800 3423 Fragments arranged by size: 3423 2492 800 243 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5620 5994 6140 387 Size: 1064 138 4031 374 146 1280 Fragments arranged by size: 4031 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3400 3400 Size: 7033 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 2724 2829 2994 3639 3653 3687 Size: 393 737 355 105 165 645 14 34 Cuts at: 3687 5096 5693 6480 6748 1239 Size: 1409 597 787 268 1524 Fragments arranged by size: 1524 1409 787 737 645 597 393 355 268 165 105 34 14 EcoNI CCTnn'n_nnAGG Cuts at: 3368 4007 4091 3368 Size: 639 84 6310 Fragments arranged by size: 6310 639 84 EcoO109I rG'GnC_Cy Cuts at: 2058 2166 2201 3259 3547 3709 3710 3725 4096 Size: 108 35 1058 288 162 1 15 371 Cuts at: 4096 2058 Size: 4995 Fragments arranged by size: 4995 1058 371 288 162 108 35 15 1 EcoRI G'AATT_C Cuts at: 2379 4342 4720 2379 Size: 1963 378 4692 Fragments arranged by size: 4692 1963 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4000 4000 Size: 7033 FseI GG_CCGG'CC Cuts at: 2052 2052 Size: 7033 FspI TGC'GCA Cuts at: 5539 5539 Size: 7033 HaeII r_GCGC'y Cuts at: 1678 2129 2163 3486 3735 4155 4317 6180 1678 Size: 451 34 1323 249 420 162 1863 2531 Fragments arranged by size: 2531 1863 1323 451 420 249 162 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2461 2493 2789 2821 3236 Size: 32 135 32 889 32 296 32 415 Cuts at: 3236 3268 3410 3442 3566 3598 5674 5706 5748 Size: 32 142 32 124 32 2076 32 42 Cuts at: 5748 5780 1373 Size: 32 2626 Fragments arranged by size: 2626 2076 889 415 296 142 135 124 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4377 4623 669 Size: 988 281 2439 246 3079 Fragments arranged by size: 3079 2439 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7033 HpaI GTT'AAC Cuts at: 4623 4623 Size: 7033 KasI G'GCGC_C Cuts at: 2125 2159 3731 2125 Size: 34 1572 5427 Fragments arranged by size: 5427 1572 34 KpnI G_GTAC'C Cuts at: 4358 4358 Size: 7033 MfeI C'AATT_G Cuts at: 4632 4632 Size: 7033 MluI A'CGCG_T Cuts at: 6909 6909 Size: 7033 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3708 6147 6331 1305 Size: 2403 2439 184 2007 Fragments arranged by size: 2439 2403 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2400 3309 10 Size: 54 1425 343 568 909 3734 Fragments arranged by size: 3734 1425 909 568 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2534 4187 4275 4991 5350 5509 Size: 648 1080 288 1653 88 716 359 159 Cuts at: 5509 518 Size: 2042 Fragments arranged by size: 2042 1653 1080 716 648 359 288 159 88 MspA1I CmG'CkG Cuts at: 1606 2044 3142 3272 4449 5126 6274 6519 1606 Size: 438 1098 130 1177 677 1148 245 2120 Fragments arranged by size: 2120 1177 1148 1098 677 438 245 130 NaeI GCC'GGC Cuts at: 2050 2124 2194 3542 2050 Size: 74 70 1348 5541 Fragments arranged by size: 5541 1348 74 70 NarI GG'CG_CC Cuts at: 2126 2160 3732 2126 Size: 34 1572 5427 Fragments arranged by size: 5427 1572 34 NcoI C'CATG_G Cuts at: 513 1065 2004 2226 2268 3264 513 Size: 552 939 222 42 996 4282 Fragments arranged by size: 4282 996 939 552 222 42 NdeI CA'TA_TG Cuts at: 387 387 Size: 7033 NgoMIV G'CCGG_C Cuts at: 2048 2122 2192 3540 2048 Size: 74 70 1348 5541 Fragments arranged by size: 5541 1348 74 70 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7033 NotI GC'GGCC_GC Cuts at: 4480 4480 Size: 7033 NspI r_CATG'y Cuts at: 1132 1229 4391 5936 1132 Size: 97 3162 1545 2229 Fragments arranged by size: 3162 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3040 3713 1348 Size: 1692 673 4668 Fragments arranged by size: 4668 1692 673 PciI A'CATG_T Cuts at: 5932 5932 Size: 7033 PflMI CCAn_nnn'nTGG Cuts at: 1108 2767 3011 4099 4252 1108 Size: 1659 244 1088 153 3889 Fragments arranged by size: 3889 1659 1088 244 153 PfoI T'CCnGG_A Cuts at: 3498 4092 3498 Size: 594 6439 PmeI GTTT'AAAC Cuts at: 4337 4337 Size: 7033 PmlI CAC'GTG Cuts at: 3313 3313 Size: 7033 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5102 5134 6615 6647 5102 Size: 32 1481 32 5488 Fragments arranged by size: 5488 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2058 3259 3725 4096 2058 Size: 1201 466 371 4995 Fragments arranged by size: 4995 1201 466 371 PshAI GACnn'nnGTC Cuts at: 1613 3512 1613 Size: 1899 5134 PsiI TTA'TAA Cuts at: 4603 4603 Size: 7033 PspOMI G'GGCC_C Cuts at: 2166 2200 3709 2166 Size: 34 1509 5490 Fragments arranged by size: 5490 1509 34 PspXI vC'TCGA_Gb Cuts at: 3676 3676 Size: 7033 PstI C_TGCA'G Cuts at: 830 2524 3034 4044 4385 830 Size: 1694 510 1010 341 3478 Fragments arranged by size: 3478 1694 1010 510 341 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7033 PvuII CAG'CTG Cuts at: 3142 3142 Size: 7033 RsrII CG'GwC_CG Cuts at: 3176 3176 Size: 7033 SacI G_AGCT'C Cuts at: 3402 3402 Size: 7033 SacII CC_GC'GG Cuts at: 3273 3273 Size: 7033 SalI G'TCGA_C Cuts at: 1936 4375 1936 Size: 2439 4594 SanDI GG'GwC_CC Cuts at: 2058 2058 Size: 7033 SapI GCTCTTCn'nnn_ Cuts at: 3845 4088 3845 Size: 243 6790 SbfI CC_TGCA'GG Cuts at: 4385 4385 Size: 7033 ScaI AGT'ACT Cuts at: 5281 6993 5281 Size: 1712 5321 SexAI A'CCwGG_T Cuts at: 4098 4098 Size: 7033 SfcI C'TryA_G Cuts at: 826 1045 2520 3030 4040 4212 4381 5516 6197 Size: 219 1475 510 1010 172 169 1135 681 Cuts at: 6197 6388 826 Size: 191 1471 Fragments arranged by size: 1475 1471 1135 1010 681 510 219 191 172 169 SfiI GGCCn_nnn'nGGCC Cuts at: 3884 3884 Size: 7033 SfoI GGC'GCC Cuts at: 2127 2161 3733 2127 Size: 34 1572 5427 Fragments arranged by size: 5427 1572 34 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 SgrAI Cr'CCGG_yG Cuts at: 1700 2122 1700 Size: 422 6611 SmaI CCC'GGG Cuts at: 1872 2056 2151 2171 4360 1872 Size: 184 95 20 2189 4545 Fragments arranged by size: 4545 2189 184 95 20 SmlI C'TyrA_G Cuts at: 820 1017 1943 3676 3992 4064 5137 6038 6300 Size: 197 926 1733 316 72 1073 901 262 Cuts at: 6300 820 Size: 1553 Fragments arranged by size: 1733 1553 1073 926 901 316 262 197 72 SnaBI TAC'GTA Cuts at: 493 4824 493 Size: 4331 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7033 SphI G_CATG'C Cuts at: 1132 4391 1132 Size: 3259 3774 SspI AAT'ATT Cuts at: 5 52 4957 5 Size: 47 4905 2081 Fragments arranged by size: 4905 2081 47 StuI AGG'CCT Cuts at: 1517 2582 3980 1517 Size: 1065 1398 4570 Fragments arranged by size: 4570 1398 1065 StyI C'CwwG_G Cuts at: 513 1065 2004 2226 2268 3264 4472 513 Size: 552 939 222 42 996 1208 3074 Fragments arranged by size: 3074 1208 996 939 552 222 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5060 5077 5230 5415 5860 325 Size: 1255 3480 17 153 185 445 1498 Fragments arranged by size: 3480 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3354 4026 5279 Size: 274 80 33 51 1443 1377 672 1253 Cuts at: 5279 6991 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1377 1253 672 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2297 2329 2770 2802 6993 7025 2297 Size: 32 441 32 4191 32 2305 Fragments arranged by size: 4191 2305 441 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2945 1316 Size: 1629 5404 XbaI T'CTAG_A Cuts at: 4369 4369 Size: 7033 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2152 2152 Size: 7033 XhoI C'TCGA_G Cuts at: 1943 3676 1943 Size: 1733 5300 XmaI C'CCGG_G Cuts at: 1870 2054 2149 2169 4358 1870 Size: 184 95 20 2189 4545 Fragments arranged by size: 4545 2189 184 95 20 XmnI GAAnn'nnTTC Cuts at: 5162 5162 Size: 7033 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4841 276 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PsrI SrfI SwaI