(Circular) (Six-base) MAPSORT of: pFN21AB3072.seq Check: 6104 from: 1 to: 6571 pFN21AB3072 6571 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3512 3743 3512 Size: 231 6340 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4381 278 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3914 1937 Size: 1977 4594 Acc65I G'GTAC_C Cuts at: 3892 3892 Size: 6571 AclI AA'CG_TT Cuts at: 1563 4698 5071 1563 Size: 3135 373 3063 Fragments arranged by size: 3135 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2633 2963 4634 6018 6286 1632 Size: 1001 330 1671 1384 268 1917 Fragments arranged by size: 1917 1671 1384 1001 330 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6374 AflIII A'CryG_T Cuts at: 1203 5470 6447 1203 Size: 4267 977 1327 Fragments arranged by size: 4267 1327 977 AgeI A'CCGG_T Cuts at: 3801 5402 3801 Size: 1601 4970 AhdI GACnn_n'nnGTC Cuts at: 3001 3564 5300 3001 Size: 563 1736 4272 Fragments arranged by size: 4272 1736 563 AleI CACnn'nnGTG Cuts at: 1166 2060 1166 Size: 894 5677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2975 3542 1184 Size: 1791 567 4213 Fragments arranged by size: 4213 1791 567 AlwNI CAG_nnn'CTG Cuts at: 2129 2370 2461 2503 2617 2800 2881 3085 3457 Size: 241 91 42 114 183 81 204 372 Cuts at: 3457 3676 4314 5886 2129 Size: 219 638 1572 2814 Fragments arranged by size: 2814 1572 638 372 241 219 204 183 114 91 81 42 ApaLI G'TGCA_C Cuts at: 3194 4628 5784 3194 Size: 1434 1156 3981 Fragments arranged by size: 3981 1434 1156 ApoI r'AATT_y Cuts at: 1493 2206 2323 3880 4106 4258 6367 1493 Size: 713 117 1557 226 152 2109 1697 Fragments arranged by size: 2109 1697 1557 713 226 152 117 AseI AT'TA_AT Cuts at: 160 1032 5125 160 Size: 872 4093 1606 Fragments arranged by size: 4093 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 2160 2909 3013 3251 3645 3896 1870 Size: 73 217 749 104 238 394 251 4545 Fragments arranged by size: 4545 749 394 251 238 217 104 73 BamHI G'GATC_C Cuts at: 3901 4268 3901 Size: 367 6204 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2338 3892 5347 618 Size: 325 217 627 267 284 1554 1455 1842 Fragments arranged by size: 1842 1554 1455 627 325 284 267 217 BanII G_rGCy'C Cuts at: 1391 2021 2123 2219 2454 2871 3626 3890 1391 Size: 630 102 96 235 417 755 264 4072 Fragments arranged by size: 4072 755 630 417 264 235 102 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5600 BbvCI CC'TCA_GC Cuts at: 2142 2142 Size: 6571 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3490 3524 4762 4796 1903 Size: 34 1553 34 1238 34 3678 Fragments arranged by size: 3678 1553 1238 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2796 3477 4463 5673 2796 Size: 681 986 1210 3694 Fragments arranged by size: 3694 1210 986 681 BclI T'GATC_A Cuts at: 1548 1548 Size: 6571 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 2982 5182 Size: 107 122 71 631 605 364 946 2200 Cuts at: 5182 136 Size: 1525 Fragments arranged by size: 2200 1525 946 631 605 364 122 107 71 BglII A'GATC_T Cuts at: 6566 6566 Size: 6571 BlpI GC'TnA_GC Cuts at: 2122 3218 3443 3988 2122 Size: 1096 225 545 4705 Fragments arranged by size: 4705 1096 545 225 Bme1580I G_kGCm'C Cuts at: 1790 2964 3198 3764 4632 5788 1790 Size: 1174 234 566 868 1156 2573 Fragments arranged by size: 2573 1174 1156 868 566 234 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6571 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2813 4302 5260 Size: 416 53 83 399 24 1396 1489 958 Cuts at: 5260 442 Size: 1753 Fragments arranged by size: 1753 1489 1396 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6571 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2305 3039 2305 Size: 734 5837 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2169 2178 2220 2438 2911 3200 3224 3343 Size: 930 9 42 218 473 289 24 119 Cuts at: 3343 3479 5231 1239 Size: 136 1752 2579 Fragments arranged by size: 2579 1752 930 473 289 218 136 119 42 24 9 Bpu10I CC'TnA_GC Cuts at: 2142 2142 Size: 6571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4696 5561 5859 4696 Size: 865 298 5408 Fragments arranged by size: 5408 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2145 2260 5234 882 Size: 617 646 115 2974 2219 Fragments arranged by size: 2974 2219 646 617 115 BsaAI yAC'GTr Cuts at: 493 1204 3589 4362 493 Size: 711 2385 773 2702 Fragments arranged by size: 2702 2385 773 711 BsaBI GATnn'nnATC Cuts at: 6565 6565 Size: 6571 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3565 4378 4760 5395 Size: 53 83 186 936 2032 813 382 635 Cuts at: 5395 275 Size: 1451 Fragments arranged by size: 2032 1451 936 813 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3801 5004 5402 5676 5823 6234 3801 Size: 1203 398 274 147 411 4138 Fragments arranged by size: 4138 1203 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6346 Fragments arranged by size: 6346 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 2099 3616 3764 1186 Size: 843 70 1517 148 3993 Fragments arranged by size: 3993 1517 843 148 70 BseYI C'CCAG_C Cuts at: 2031 2155 2692 2739 2874 2972 3163 5774 6270 Size: 124 537 47 135 98 191 2611 496 Cuts at: 6270 2031 Size: 2332 Fragments arranged by size: 2611 2332 537 496 191 135 124 98 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2053 2786 3071 3635 1163 Size: 573 317 733 285 564 4099 Fragments arranged by size: 4099 733 573 564 317 285 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4021 4782 5810 1540 Size: 275 186 2020 761 1028 2301 Fragments arranged by size: 2301 2020 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2454 3198 3626 3687 3864 4632 4717 5788 2454 Size: 744 428 61 177 768 85 1071 3237 Fragments arranged by size: 3237 1071 768 744 428 177 85 61 BsmI GAATG_Cn' Cuts at: 4082 4175 4082 Size: 93 6478 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2123 2219 2454 2871 2964 3198 Size: 399 231 102 96 235 417 93 234 Cuts at: 3198 3626 3687 3764 3864 3890 4632 4717 5788 Size: 428 61 77 100 26 742 85 1071 Cuts at: 5788 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 428 417 399 235 234 231 102 100 96 93 85 77 61 26 BspEI T'CCGG_A Cuts at: 6234 6234 Size: 6571 BspHI T'CATG_A Cuts at: 2466 2685 4460 6190 2466 Size: 219 1775 1730 2847 Fragments arranged by size: 2847 1775 1730 219 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2656 3006 3512 3743 3926 844 Size: 1812 350 506 231 183 3489 Fragments arranged by size: 3489 1812 506 350 231 183 BsrBI CCG'CTC Cuts at: 1394 1823 3175 3319 4458 1394 Size: 429 1352 144 1139 3507 Fragments arranged by size: 3507 1352 1139 429 144 BsrDI GCAATG_nn' Cuts at: 65 1248 5066 5240 65 Size: 1183 3818 174 1396 Fragments arranged by size: 3818 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3447 3801 5215 5402 1700 Size: 110 1637 354 1414 187 2869 Fragments arranged by size: 2869 1637 1414 354 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6571 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6571 BssSI C'ACGA_G Cuts at: 2105 4631 5643 2105 Size: 2526 1012 3033 Fragments arranged by size: 3033 2526 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2800 3085 794 Size: 2006 285 4280 Fragments arranged by size: 4280 2006 285 BstBI TT'CG_AA Cuts at: 5375 5375 Size: 6571 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6571 BstYI r'GATC_y Cuts at: 1972 2658 3327 3372 3380 3901 4268 4653 4670 Size: 686 669 45 8 521 367 385 17 Cuts at: 4670 6122 6208 6220 6566 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 686 669 521 385 367 346 86 45 17 12 8 BtgI C'CryG_G Cuts at: 513 1065 2058 2841 2977 3488 513 Size: 552 993 783 136 511 3596 Fragments arranged by size: 3596 993 783 552 511 136 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3858 487 Size: 683 56 2632 3200 Fragments arranged by size: 3200 2632 683 56 BtsI GCAGTG_nn' Cuts at: 2127 4087 4881 4901 2127 Size: 1960 794 20 3797 Fragments arranged by size: 3797 1960 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6571 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6536 DraI TTT'AAA Cuts at: 3875 3875 Size: 6571 DraIII CAC_nnn'GTG Cuts at: 3781 3781 Size: 6571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5578 809 Size: 507 4262 1802 Fragments arranged by size: 4262 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2844 3607 4018 Size: 54 1425 50 275 18 1014 763 411 Cuts at: 4018 4907 6536 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1014 889 763 411 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4018 1537 Size: 275 2206 4090 Fragments arranged by size: 4090 2206 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2822 3828 3833 4501 1353 Size: 1469 1006 5 668 3423 Fragments arranged by size: 3423 1469 1006 668 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5158 5532 5678 387 Size: 1064 138 3569 374 146 1280 Fragments arranged by size: 3569 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2452 3624 2452 Size: 1172 5399 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2169 2178 2220 2438 2633 2911 2963 Size: 393 537 9 42 218 195 278 52 Cuts at: 2963 3200 3224 3343 3479 4634 5231 6018 6286 Size: 237 24 119 136 1155 597 787 268 Cuts at: 6286 1239 Size: 1524 Fragments arranged by size: 1524 1155 787 597 537 393 278 268 237 218 195 136 119 52 42 24 9 EcoNI CCTnn'n_nnAGG Cuts at: 3746 3746 Size: 6571 EcoO109I rG'GnC_Cy Cuts at: 2110 2250 2424 2905 2984 2995 3102 3139 3547 Size: 140 174 481 79 11 107 37 408 Cuts at: 3547 3558 2110 Size: 11 5123 Fragments arranged by size: 5123 481 408 174 140 107 79 37 11 11 EcoRI G'AATT_C Cuts at: 3880 4258 3880 Size: 378 6193 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2329 2329 Size: 6571 FspI TGC'GCA Cuts at: 5077 5077 Size: 6571 HaeII r_GCGC'y Cuts at: 1678 5718 1678 Size: 4040 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2216 2248 2987 3019 3550 Size: 32 135 32 644 32 739 32 531 Cuts at: 3550 3582 3761 3793 3806 3838 5212 5244 5286 Size: 32 179 32 13 32 1374 32 42 Cuts at: 5286 5318 1373 Size: 32 2626 Fragments arranged by size: 2626 1374 739 644 531 179 135 42 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 2671 3730 3915 4161 669 Size: 988 281 733 1059 185 246 3079 Fragments arranged by size: 3079 1059 988 733 281 246 185 HindIII A'AGCT_T Cuts at: 748 748 Size: 6571 HpaI GTT'AAC Cuts at: 2671 4161 2671 Size: 1490 5081 KpnI G_GTAC'C Cuts at: 3896 3896 Size: 6571 MfeI C'AATT_G Cuts at: 4170 4170 Size: 6571 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2259 5685 5869 1305 Size: 954 3426 184 2007 Fragments arranged by size: 3426 2007 954 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2846 3609 10 Size: 54 1425 343 1014 763 2972 Fragments arranged by size: 2972 1425 1014 763 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2060 3719 4529 4888 5047 518 Size: 648 843 51 1659 810 359 159 2042 Fragments arranged by size: 2042 1659 843 810 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2035 2135 2479 2617 2743 2791 2797 2955 Size: 429 100 344 138 126 48 6 158 Cuts at: 2955 3082 3490 3664 3987 4664 5812 6057 1606 Size: 127 408 174 323 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 429 408 344 323 245 174 158 138 127 126 100 48 6 NaeI GCC'GGC Cuts at: 3449 3449 Size: 6571 NcoI C'CATG_G Cuts at: 513 1065 2058 2841 2977 513 Size: 552 993 783 136 4107 Fragments arranged by size: 4107 993 783 552 136 NdeI CA'TA_TG Cuts at: 387 387 Size: 6571 NgoMIV G'CCGG_C Cuts at: 3447 3447 Size: 6571 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6571 NotI GC'GGCC_GC Cuts at: 4018 4018 Size: 6571 NspI r_CATG'y Cuts at: 1132 1229 2677 3352 3661 3718 3929 5474 1132 Size: 97 1448 675 309 57 211 1545 2229 Fragments arranged by size: 2229 1545 1448 675 309 211 97 57 PasI CC'CwG_GG Cuts at: 1348 2116 2200 1348 Size: 768 84 5719 Fragments arranged by size: 5719 768 84 PciI A'CATG_T Cuts at: 5470 5470 Size: 6571 PflMI CCAn_nnn'nTGG Cuts at: 1108 2370 2461 1108 Size: 1262 91 5218 Fragments arranged by size: 5218 1262 91 PfoI T'CCnGG_A Cuts at: 3066 3066 Size: 6571 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4640 4672 6153 6185 4640 Size: 32 1481 32 5026 Fragments arranged by size: 5026 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2250 2424 2995 3139 3558 2250 Size: 174 571 144 419 5263 Fragments arranged by size: 5263 571 419 174 144 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6571 PsiI TTA'TAA Cuts at: 4141 4141 Size: 6571 PstI C_TGCA'G Cuts at: 830 2796 2865 3003 3740 3923 830 Size: 1966 69 138 737 183 3478 Fragments arranged by size: 3478 1966 737 183 138 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 PvuII CAG'CTG Cuts at: 2035 2479 2617 2743 2797 2955 3082 2035 Size: 444 138 126 54 158 127 5524 Fragments arranged by size: 5524 444 158 138 127 126 54 SacI G_AGCT'C Cuts at: 2454 3626 2454 Size: 1172 5399 SacII CC_GC'GG Cuts at: 3491 3491 Size: 6571 SalI G'TCGA_C Cuts at: 1936 3913 1936 Size: 1977 4594 SanDI GG'GwC_CC Cuts at: 2250 2424 2250 Size: 174 6397 SapI GCTCTTCn'nnn_ Cuts at: 3833 3833 Size: 6571 SbfI CC_TGCA'GG Cuts at: 2865 3923 2865 Size: 1058 5513 ScaI AGT'ACT Cuts at: 4819 6531 4819 Size: 1712 4859 SfcI C'TryA_G Cuts at: 826 1045 2227 2792 2861 2999 3736 3919 5054 Size: 219 1182 565 69 138 737 183 1135 Cuts at: 5054 5735 5926 826 Size: 681 191 1471 Fragments arranged by size: 1471 1182 1135 737 681 565 219 191 183 138 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6571 SmaI CCC'GGG Cuts at: 1872 2162 3898 1872 Size: 290 1736 4545 Fragments arranged by size: 4545 1736 290 SmlI C'TyrA_G Cuts at: 820 1017 1943 4675 5576 5838 820 Size: 197 926 2732 901 262 1553 Fragments arranged by size: 2732 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4362 493 Size: 3869 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 3661 3929 1132 Size: 2529 268 3774 Fragments arranged by size: 3774 2529 268 SrfI GCCC'GGGC Cuts at: 2162 2162 Size: 6571 SspI AAT'ATT Cuts at: 5 52 4495 5 Size: 47 4443 2081 Fragments arranged by size: 4443 2081 47 StuI AGG'CCT Cuts at: 1517 2833 1517 Size: 1316 5255 StyI C'CwwG_G Cuts at: 513 1065 2058 2841 2901 2977 3034 3105 3168 Size: 552 993 783 60 76 57 71 63 Cuts at: 3168 3309 3376 4010 513 Size: 141 67 634 3074 Fragments arranged by size: 3074 993 783 634 552 141 76 71 67 63 60 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2864 3213 4598 4615 4768 4953 5398 Size: 1255 1284 349 1385 17 153 185 445 Cuts at: 5398 325 Size: 1498 Fragments arranged by size: 1498 1385 1284 1255 445 349 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3480 3540 4817 Size: 274 80 33 51 1443 1503 60 1277 Cuts at: 4817 6529 96 Size: 1712 138 Fragments arranged by size: 1712 1503 1443 1277 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2465 2497 6531 6563 2465 Size: 32 4034 32 2473 Fragments arranged by size: 4034 2473 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3050 1316 Size: 1734 4837 XbaI T'CTAG_A Cuts at: 3907 3907 Size: 6571 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3175 3175 Size: 6571 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6571 XmaI C'CCGG_G Cuts at: 1870 2160 3896 1870 Size: 290 1736 4545 Fragments arranged by size: 4545 1736 290 XmnI GAAnn'nnTTC Cuts at: 3293 4700 3293 Size: 1407 5164 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4379 276 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SexAI SfiI SfoI SwaI